KEGG ORTHOLOGY: K04128

ORTHOLOGY: K04128

Entry

K04128                      KO

Symbol

cmcH

Name

hydroxymethyl cephem carbamoyltransferase [EC:2.1.3.7]

Pathway

map00311

Penicillin and cephalosporin biosynthesis

map01100

Metabolic pathways

map01110

Biosynthesis of secondary metabolites

Module

M00673

Cephamycin C biosynthesis, aminoadipate + cycteine + valine => cephamycin C

Reaction

R05230

carbamoyl-phosphate:deacetylcephalosporin C carbamoyltransferase

Brite

KEGG Orthology (KO) [BR:ko00001]
09100 Metabolism
  09110 Biosynthesis of other secondary metabolites
   00311 Penicillin and cephalosporin biosynthesis
    K04128  cmcH; hydroxymethyl cephem carbamoyltransferase
09180 Brite Hierarchies
  09181 Protein families: metabolism
   01008 Polyketide biosynthesis proteins
    K04128  cmcH; hydroxymethyl cephem carbamoyltransferase
Enzymes [BR:ko01000]
2. Transferases
  2.1  Transferring one-carbon groups
   2.1.3  Carboxy- and carbamoyltransferases
    2.1.3.7  3-hydroxymethylcephem carbamoyltransferase
     K04128  cmcH; hydroxymethyl cephem carbamoyltransferase
Polyketide biosynthesis proteins [BR:ko01008]
Nonribosomal peptide tailoring proteins
  Others
   K04128  cmcH; hydroxymethyl cephem carbamoyltransferase

Other DBs

GO:

0047155

Genes

SANL:

KZO11_35585

SCLF:

BB341_07765

SFIY:

F0344_19625

SCT:	SCAT_5682(cmcH)

SCY:	SCATT_56820

THAO:

NI17_008625

AAB:	A4R43_03245

AMYY:

YIM_39160(novN2)

Reference

PMID:7557411

Authors

Coque JJ, Perez-Llarena FJ, Enguita FJ, Fuente JL, Martin JF, Liras P

Title

Characterization of the cmcH genes of Nocardia lactamdurans and Streptomyces clavuligerus encoding a functional 3'-hydroxymethylcephem O-carbamoyltransferase for cephamycin biosynthesis.

Journal

Gene 162:21-7 (1995)
DOI:10.1016/0378-1119(95)00308-s

Reference

PMID:9696752

Authors

Alexander DC, Jensen SE

Title

Investigation of the Streptomyces clavuligerus cephamycin C gene cluster and its regulation by the CcaR protein.

Journal

J Bacteriol 180:4068-79 (1998)
DOI:10.1128/JB.180.16.4068-4079.1998

Sequence

[sclf:BB341_07765]

LinkDB

ENZYME: 2.1.3.7

Entry

EC 2.1.3.7                  Enzyme

Name

3-hydroxymethylcephem carbamoyltransferase

Class

Transferases;
Transferring one-carbon groups;
Carboxy- and carbamoyltransferases

Sysname

carbamoyl-phosphate:3-hydroxymethylceph-3-em-4-carboxylate carbamoyltransferase

Reaction(IUBMB)

carbamoyl phosphate + a 3-hydroxymethylceph-3-em-4-carboxylate = phosphate + a 3-carbamoyloxymethylcephem [RN:R04281]

Reaction(KEGG)

R04281 > R05230

Substrate

carbamoyl phosphate [CPD:C00169];
3-hydroxymethylceph-3-em-4-carboxylate

Product

phosphate [CPD:C00009];
3-carbamoyloxymethylcephem [CPD:C03674]

Comment

Acts on a wide range of 3-hydroxymethylcephems (a subclass of the cephalosporin antibiotics). Activated by ATP.

History

EC 2.1.3.7 created 1983

Pathway

ec00311

Penicillin and cephalosporin biosynthesis

ec01100

Metabolic pathways

ec01110

Biosynthesis of secondary metabolites

Orthology

K04128

hydroxymethyl cephem carbamoyltransferase

Genes

SANL:

KZO11_35585

SCLF:

BB341_07765

SFIY:

F0344_19625

SCT:	SCAT_5682(cmcH)

SCY:	SCATT_56820

THAO:

NI17_008625

AAB:	A4R43_03245

AMYY:

YIM_39160(novN2)

Reference

1 [PMID:6248024]

Authors

Brewer SJ, Taylor PM, Turner MK.

Title

An adenosine triphosphate-dependent carbamoylphosphate--3-hydroxymethylcephem O-carbamoyltransferase from Streptomyces clavuligerus.

Journal

Biochem J 185:555-64 (1980)
DOI:10.1042/bj1850555

Other DBs

ExplorEnz - The Enzyme Database:

2.1.3.7

IUBMB Enzyme Nomenclature:

2.1.3.7

ExPASy - ENZYME nomenclature database:

2.1.3.7

BRENDA, the Enzyme Database:

2.1.3.7

CAS:	74315-96-1

LinkDB

REACTION: R05230

Entry

R05230                      Reaction

Name

carbamoyl-phosphate:deacetylcephalosporin C carbamoyltransferase

Definition

Deacetylcephalosporin C + Carbamoyl phosphate <=> O-Carbamoyl-deacetylcephalosporin C + Orthophosphate

Equation

C03112 + C00169 <=> C06568 + C00009

Reaction class

RC00041

C03112_C06568

Enzyme

2.1.3.7

Pathway

rn00311

Penicillin and cephalosporin biosynthesis

rn01100

Metabolic pathways

rn01110

Biosynthesis of secondary metabolites

Module

M00673

Cephamycin C biosynthesis, aminoadipate + cycteine + valine => cephamycin C

Brite

Enzymatic reactions [BR:br08201]
2. Transferase reactions
  2.1  Transferring one-carbon groups
   2.1.3  Carboxy- and carbamoyltransferases
    2.1.3.7
     R05230  Deacetylcephalosporin C + Carbamoyl phosphate <=> O-Carbamoyl-deacetylcephalosporin C + Orthophosphate
IUBMB reaction hierarchy [BR:br08202]
2. Transferase reactions
  2.1.3.7
   R04281  3-Hydroxymethyl ceph-3-em-4-carboxylate <=> 3-Carbamoyloxymethylcephem
    R05230  Deacetylcephalosporin C <=> O-Carbamoyl-deacetylcephalosporin C

Orthology

K04128

hydroxymethyl cephem carbamoyltransferase [EC:2.1.3.7]

LinkDB

DBGET integrated database retrieval system