KEGG   ORTHOLOGY: K07540
Entry
K07540                      KO                                     
Symbol
E4.1.99.11
Name
benzylsuccinate synthase [EC:4.1.99.11]
Pathway
map00623  Toluene degradation
map01100  Metabolic pathways
map01120  Microbial metabolism in diverse environments
map01220  Degradation of aromatic compounds
Module
M00418  Toluene degradation, anaerobic, toluene => benzoyl-CoA
Reaction
R05598  benzylsuccinate fumarate-lyase
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09111 Xenobiotics biodegradation and metabolism
   00623 Toluene degradation
    K07540  E4.1.99.11; benzylsuccinate synthase
Enzymes [BR:ko01000]
 4. Lyases
  4.1  Carbon-carbon lyases
   4.1.99  Other carbon-carbon lyases
    4.1.99.11  benzylsuccinate synthase
     K07540  E4.1.99.11; benzylsuccinate synthase
Other DBs
GO: 0018805
Genes
EBA: c2A303(bssC) c2A304(bssA) c2A305(bssB)
APET: ToN1_01380(bssC) ToN1_01390(bssA) ToN1_01400(bssB)
AZI: AzCIB_4504 AzCIB_4505 AzCIB_4506
AZD: CDA09_07530 CDA09_07535 CDA09_21890 CDA09_21895
TCL: Tchl_1404 Tchl_1405 Tchl_1406
TAK: Tharo_3069 Tharo_3070 Tharo_3071
GME: Gmet_1538(bssB) Gmet_1539(bssA) Gmet_1540(bssC)
GEO: Geob_2437(bssB-2) Geob_2438(bssA-2) Geob_2439(bssC-2) Geob_2447(bssB-1) Geob_2448(bssA-1) Geob_2449(bssC-1)
DAL: Dalk_2198
DTO: TOL2_C01390(bssB) TOL2_C01400(bssA) TOL2_C01410(bssC)
DOV: DSCO28_00220 DSCO28_15650 DSCO28_65240(pflD_2)
DWD: DSCW_52810
DALK: DSCA_19210
DMM: dnm_089690(hbsB) dnm_089710(hbsC)
DGI: Desgi_0659 Desgi_0660
AG: CAA05051(bssC) CAA05052(bssA) CAA05053(bssB)
 » show all
Reference
PMID:9632263
  Authors
Leuthner B, Leutwein C, Schulz H, Horth P, Haehnel W, Schiltz E, Schagger H, Heider J
  Title
Biochemical and genetic characterization of benzylsuccinate synthase from Thauera aromatica: a new glycyl radical enzyme catalysing the first step in anaerobic toluene metabolism.
  Journal
Mol Microbiol 28:615-28 (1998)
DOI:10.1046/j.1365-2958.1998.00826.x
  Sequence
[ag:CAA05052 CAA05053 CAA05051]
LinkDB

KEGG   ENZYME: 4.1.99.11
Entry
EC 4.1.99.11                Enzyme                                 
Name
benzylsuccinate synthase;
benzylsuccinate fumarate-lyase
Class
Lyases;
Carbon-carbon lyases;
Other carbon-carbon lyases
Sysname
benzylsuccinate fumarate-lyase (toluene-forming)
Reaction(IUBMB)
benzylsuccinate = toluene + fumarate [RN:R05598]
Reaction(KEGG)
R05598
Substrate
benzylsuccinate [CPD:C09816]
Product
toluene [CPD:C01455];
fumarate [CPD:C00122]
Comment
A glycyl radical enzyme that is inhibited by benzyl alcohol, benzaldehyde, phenylhydrazine and is inactivated by oxygen.
History
EC 4.1.99.11 created 2000
Pathway
ec00623  Toluene degradation
ec01100  Metabolic pathways
ec01120  Microbial metabolism in diverse environments
Orthology
K07540  benzylsuccinate synthase
Genes
EBA: c2A303(bssC) c2A304(bssA) c2A305(bssB)
APET: ToN1_01380(bssC) ToN1_01390(bssA) ToN1_01400(bssB)
AZI: AzCIB_4504 AzCIB_4505 AzCIB_4506
AZD: CDA09_07530 CDA09_07535 CDA09_21890 CDA09_21895
TCL: Tchl_1404 Tchl_1405 Tchl_1406
TAK: Tharo_3069 Tharo_3070 Tharo_3071
GME: Gmet_1538(bssB) Gmet_1539(bssA) Gmet_1540(bssC)
GEO: Geob_2437(bssB-2) Geob_2438(bssA-2) Geob_2439(bssC-2) Geob_2447(bssB-1) Geob_2448(bssA-1) Geob_2449(bssC-1)
DAL: Dalk_2198
DTO: TOL2_C01390(bssB) TOL2_C01400(bssA) TOL2_C01410(bssC)
DOV: DSCO28_00220 DSCO28_15650 DSCO28_65240(pflD_2)
DWD: DSCW_52810
DALK: DSCA_19210
DMM: dnm_089690(hbsB) dnm_089710(hbsC)
DGI: Desgi_0659 Desgi_0660
 » show all
Reference
1  [PMID:9765580]
  Authors
Beller HR, Spormann AM.
  Title
Analysis of the novel benzylsuccinate synthase reaction for anaerobic toluene activation based on structural studies of the product.
  Journal
J Bacteriol 180:5454-7 (1998)
DOI:10.1128/JB.180.20.5454-5457.1998
Reference
2  [PMID:9632263]
  Authors
Leuthner B, Leutwein C, Schulz H, Horth P, Haehnel W, Schiltz E, Schagger H, Heider J
  Title
Biochemical and genetic characterization of benzylsuccinate synthase from Thauera aromatica: a new glycyl radical enzyme catalysing the first step in anaerobic toluene metabolism.
  Journal
Mol Microbiol 28:615-28 (1998)
DOI:10.1046/j.1365-2958.1998.00826.x
  Sequence
[ag:CAA05052 CAA05053 CAA05051]
Other DBs
ExplorEnz - The Enzyme Database: 4.1.99.11
IUBMB Enzyme Nomenclature: 4.1.99.11
ExPASy - ENZYME nomenclature database: 4.1.99.11
UM-BBD (Biocatalysis/Biodegradation Database): 4.1.99.11
BRENDA, the Enzyme Database: 4.1.99.11
CAS: 209264-18-6
LinkDB

KEGG   REACTION: R05598
Entry
R05598                      Reaction                               
Name
benzylsuccinate fumarate-lyase
Definition
Toluene + Fumarate <=> Benzylsuccinate
Equation
C01455 + C00122 <=> C09816
Comment
BssA,BssB,BssC
Reaction class
RC01433  C00122_C09816
RC01434  C01455_C09816
Enzyme
4.1.99.11
Pathway
rn00623  Toluene degradation
rn01100  Metabolic pathways
rn01120  Microbial metabolism in diverse environments
rn01220  Degradation of aromatic compounds
Module
M00418  Toluene degradation, anaerobic, toluene => benzoyl-CoA
Brite
Enzymatic reactions [BR:br08201]
 4. Lyase reactions
  4.1  Carbon-carbon lyases
   4.1.99  Other carbon-carbon lyases
    4.1.99.11
     R05598  Toluene + Fumarate <=> Benzylsuccinate
Orthology
K07540  benzylsuccinate synthase [EC:4.1.99.11]
Other DBs
RHEA: 10419
LinkDB

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