KEGG ORTHOLOGY: K09832

ORTHOLOGY: K09832

Entry

K09832 KO

Symbol

CYP710A

Name

sterol 22-desaturase [EC:1.14.19.41]

Pathway

map00100

Steroid biosynthesis

map01100

Metabolic pathways

map01110

Biosynthesis of secondary metabolites

Brite

KEGG Orthology (KO) [BR:ko00001]
09100 Metabolism
  09103 Lipid metabolism
   00100 Steroid biosynthesis
    K09832  CYP710A; sterol 22-desaturase
09180 Brite Hierarchies
  09181 Protein families: metabolism
   00199 Cytochrome P450
    K09832  CYP710A; sterol 22-desaturase
Enzymes [BR:ko01000]
1. Oxidoreductases
  1.14  Acting on paired donors, with incorporation or reduction of molecular oxygen
   1.14.19  With oxidation of a pair of donors resulting in the reduction of O2 to two molecules of water
    1.14.19.41  sterol 22-desaturase
     K09832  CYP710A; sterol 22-desaturase
Cytochrome P450 [BR:ko00199]
Cytochrome P450, plant type
  CYP710 family
   K09832  CYP710A; sterol 22-desaturase

Other DBs

RN:	R07489 R11097

COG:

COG2124

GO:

0000249

Genes

ATH:	AT2G28850(CYP710A3) AT2G28860(CYP710A4) AT2G34490(CYP710A2) AT2G34500(CYP710A1)

ALY:	9317081 9317405 9317406

CRB:	17887986 17889424 17889482

CSAT:

104699232 104699233 104700630 104750004 104750006 104753438 104781842 104781843 104786110 104786111 104786814 104789238 104789239 104789240 104792202 104792203

EUS:	EUTSA_v10016547mg EUTSA_v10016552mg

BRP:	103865343 103867449

BNA:	106396203 106432759 106432788 106447495

BOE:	106339100 106339917 106340751 106341405

RSZ:	108807244 108833237 108852806

THJ:

104814293

CPAP:

110807637

CIT:

102618952

CIC:	CICLE_v10014973mg

PVY:

116141721

TCC:

18596516

GRA:	105769297 105769298 105772027 105774040

GHI:	107901888 107905877 107905878 107942637 121203644

GAB:	108452825 108452842

DZI:	111280976 111318668

EGR:

104440796

GMX:	100787616 100808148

GSJ:	114377625 114387884

PVU:	PHAVU_006G163800g

VRA:

VAR:

VUN:

CCAJ:

APRC:

MTR:	MTR_2g019640

CAM:

101489453

ADU:

107468177

AIP:

107623676

AHF:	112714308 112782610

LANG:

109328906 109329075 109345270

FVE:

RCN:

PPER:

PMUM:

PAVI:

PDUL:

MDM:

ZJU:

MNT:

CSV:

CMO:

BHJ:

CMAX:

CMOS:

CPEP:

HBR:	110640583 110645097

MESC:

POP:

PEU:

PALZ:

JRE:

QSU:

QLO:	115980600 115992293

TWL:

119995149

VVI:

100255505

VRI:

117922864

SLY:	100136886(SD1)

SPEN:

107011895

SOT:

102581211

CANN:

107859112

NTA:	107763057 107771426(SD1)

NSY:

NTO:

NAU:

INI:

ITR:

SIND:

OEU:	111407278 111412227

EGT:

105951371

SSPL:

121805298 121808148

HAN:	110911670 110937398

ECAD:

LSV:

CCAV:

DCR:

CSIN:

BVG:

SOE:	110778797 110778903

CQI:	110730201 110732522

NNU:

104597733

MING:

122060078 122092751

PSOM:

113329902 113338741 113345304 113355287

NCOL:

116265070

OSA:	4325562 4325563 4325564 9267882

DOSA:

Os01t0210900-01(Os01g0210900) Os01t0211000-00(Os01g0211000) Os01t0211200-00(Os01g0211200) Os01t0211600-01(Os01g0211600)

OBR:	102709230 102711741

BDI:

100827562

ATS:	109746138 109757150 109772439

TDC:	119267541 119275444 119292328 119298617 119304413 119338528 119338529 119343557 119344120

SBI:

ZMA:

SITA:

PVIR:

PHAI:

PDA:	103702545 103703021

EGU:	105040643 105048162

MUS:	103995072 103999677 104000847

DCT:

PEQ:

AOF:

ATR:

SMO:	SELMODRAFT_234389 SELMODRAFT_75218(CYP710A21)

PPP:	112277322 112285903

» show all

Reference

PMID:16531502 (CYP710A1 CYP710A2)

Authors

Morikawa T, Mizutani M, Aoki N, Watanabe B, Saga H, Saito S, Oikawa A, Suzuki H, Sakurai N, Shibata D, Wadano A, Sakata K, Ohta D.

Title

Cytochrome P450 CYP710A encodes the sterol C-22 desaturase in Arabidopsis and tomato.

Journal

Plant Cell 18:1008-22 (2006)
DOI:10.1105/tpc.105.036012

Sequence

[ath:AT2G34500 AT2G34490]

LinkDB

ENZYME: 1.14.19.41

Entry

EC 1.14.19.41 Enzyme

Name

sterol 22-desaturase;
ERG5 (gene name);
CYP710A (gene name)

Class

Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With oxidation of a pair of donors resulting in the reduction of O2 to two molecules of water

Sysname

ergosta-5,7,24(28)-trien-3beta-ol,NADPH:oxygen oxidoreductase (22,23-dehydrogenating)

Reaction(IUBMB)

ergosta-5,7,24(28)-trien-3beta-ol + NADPH + H+ + O2 = ergosta-5,7,22,24(28)-tetraen-3beta-ol + NADP+ + 2 H2O [RN:R07506]

Reaction(KEGG)

R07506;
(other) R07489 R11097

Substrate

ergosta-5,7,24(28)-trien-3beta-ol [CPD:C15778];
NADPH [CPD:C00005];
H+ [CPD:C00080];
O2 [CPD:C00007]

Product

ergosta-5,7,22,24(28)-tetraen-3beta-ol [CPD:C05440];
NADP+ [CPD:C00006];
H2O [CPD:C00001]

Comment

A heme-thiolate protein (P-450). The enzyme, found in yeast and plants, catalyses the introduction of a double bond between the C-22 and C-23 carbons of certain sterols. In yeast the enzyme acts on ergosta-5,7,24(28)-trien-3beta-ol, a step in the biosynthesis of ergosterol. The enzyme from the plant Arabidopsis thaliana acts on sitosterol and 24-epi-campesterol, producing stigmasterol and brassicasterol, respectively.

History

EC 1.14.19.41 created 2015

Pathway

ec00100

Steroid biosynthesis

ec01100

Metabolic pathways

ec01110

Biosynthesis of secondary metabolites

Orthology

K09831

sterol 22-desaturase

K09832

sterol 22-desaturase

Genes

ATH:	AT2G28850(CYP710A3) AT2G28860(CYP710A4) AT2G34490(CYP710A2) AT2G34500(CYP710A1)

ALY:	9317081 9317405 9317406

CRB:	17887986 17889424 17889482

CSAT:

104699232 104699233 104700630 104750004 104750006 104753438 104781842 104781843 104786110 104786111 104786814 104789238 104789239 104789240 104792202 104792203

EUS:	EUTSA_v10016547mg EUTSA_v10016552mg

BRP:	103865343 103867449

BNA:	106396203 106432759 106432788 106447495

BOE:	106339100 106339917 106340751 106341405

RSZ:	108807244 108833237 108852806

THJ:

104814293

CPAP:

110807637

CIT:

102618952

CIC:	CICLE_v10014973mg

PVY:

116141721

TCC:

18596516

GRA:	105769297 105769298 105772027 105774040

GHI:	107901888 107905877 107905878 107942637 121203644

GAB:	108452825 108452842

DZI:	111280976 111318668

EGR:

104440796

GMX:	100787616 100808148

GSJ:	114377625 114387884

PVU:	PHAVU_006G163800g

VRA:

VAR:

VUN:

CCAJ:

APRC:

MTR:	MTR_2g019640

CAM:

101489453

ADU:

107468177

AIP:

107623676

AHF:	112714308 112782610

LANG:

109328906 109329075 109345270

FVE:

RCN:

PPER:

PMUM:

PAVI:

PDUL:

MDM:

ZJU:

MNT:

CSV:

CMO:

BHJ:

CMAX:

CMOS:

CPEP:

HBR:	110640583 110645097

MESC:

POP:

PEU:

PALZ:

JRE:

QSU:	112004265 112009839 112037010

QLO:	115980600 115992293

TWL:

119995149

VVI:

100255505

VRI:

117922864

SLY:	100136886(SD1)

SPEN:

107011895

SOT:

102581211

CANN:

107859112

NTA:	107763057 107771426(SD1)

NSY:

NTO:

NAU:

INI:

ITR:

SIND:

OEU:	111407278 111412227

EGT:

105951371

SSPL:

121805298 121808148

HAN:	110911670 110937398

ECAD:

LSV:

CCAV:

DCR:

CSIN:

BVG:

SOE:	110778797 110778903

CQI:	110730201 110732522

NNU:

104597733

MING:

122060078 122092751

PSOM:

113329902 113338741 113345304 113355287

NCOL:

116265070

OSA:	4325562 4325563 4325564 9267882

DOSA:

Os01t0210900-01(Os01g0210900) Os01t0211000-00(Os01g0211000) Os01t0211200-00(Os01g0211200) Os01t0211600-01(Os01g0211600)

OBR:	102709230 102711741

BDI:

100827562

ATS:	109746138 109757150 109772439

TDC:	119267541 119275444 119292328 119298617 119304413 119338528 119338529 119343557 119344120

SBI:

ZMA:

SITA:

PVIR:

PHAI:

PDA:	103702545 103703021

EGU:	105040643 105048162

MUS:	103995072 103999677 104000847

DCT:

PEQ:

AOF:

ATR:

SMO:	SELMODRAFT_234389 SELMODRAFT_75218(CYP710A21)

PPP:	112277322 112285903

SCE:	YMR015C(ERG5)

AGO:	AGOS_ABR053C

KLA:	KLLA0_D11242g

KMX:	KLMA_60417(cyp524A1)

LTH:	KLTH0D07832g

VPO:	Kpol_1056p10

ZRO:	ZYRO0E06204g

CGR:	CAGL0M07656g

NCS:	NCAS_0A12700(NCAS0A12700)

NDI:	NDAI_0B01560(NDAI0B01560)

TPF:	TPHA_0I02420(TPHA0I02420)

TBL:	TBLA_0H02650(TBLA0H02650)

TDL:	TDEL_0B06510(TDEL0B06510)

TGB:	HG536_0G01250

KAF:	KAFR_0A02110(KAFR0A02110)

ZMK:	HG535_0G03560

PPA:	PAS_chr3_0507

DHA:	DEHA2D06182g

PIC:	PICST_73043(ERG5)

PGU:	PGUG_05085

SPAA:

SPAPADRAFT_63544(ERG5)

LEL:	LELG_05188

CAL:	CAALFM_C702840CA(ERG5)

CTP:	CTRG_04983

COT:	CORT_0G04140

CDU:	CD36_72510

CTEN:

CANTEDRAFT_115386

YLI:	YALI0A18062g

CLU:	CLUG_04246

CLUS:

A9F13_04g02805

CAUR:

CJI97_001893

SLB:	AWJ20_75(ERG5)

PKZ:	C5L36_0B12520

BNN:	FOA43_004793(ERG5)

BBRX:

BRETT_002777(ERG5)

NCR:

NCU05278

NTE:	NEUTE1DRAFT116692(NEUTE1DRAFT_116692)

SMP:	SMAC_01710

PAN:	PODANSg6262 PODANSg7995

TTT:	THITE_128412

MTM:	MYCTH_73679

CTHR:

CTHT_0029760

MGR:

MGG_06047

TMN:	UCRPA7_7946

SSCK:

SPSK_00476

FGR:	FGSG_01959 FGSG_03686

FPU:	FPSE_01847 FPSE_09181

FVR:	FVEG_07284 FVEG_08786

FOX:	FOXG_04166 FOXG_09685

NHE:	NECHADRAFT_72313 NECHADRAFT_72652

TRE:	TRIREDRAFT_122166 TRIREDRAFT_58689

TRR:	M419DRAFT_141218 M419DRAFT_74177

MAW:

MAC_08636

MAJ:

MAA_01109

CMT:

CCM_02962

PLJ:	VFPFJ_00660 VFPFJ_08344

VAL:	VDBG_00192 VDBG_00755

VDA:	VDAG_00367 VDAG_08476

CFJ:	CFIO01_05732 CFIO01_13734

SAPO:

SAPIO_CDS4161 SAPIO_CDS7005

ELA:	UCREL1_11694

PFY:	PFICI_04403 PFICI_13034

SSL:	SS1G_09832

BFU:	BCIN_07g01120(Bcerg5)

MBE:

MBM_06688

PSCO:

LY89DRAFT_257563 LY89DRAFT_584410

GLZ:	GLAREA_02023 GLAREA_06590

ANI:

AN4042.2

AFM:	AFUA_1G03950

ACT:	ACLA_008020 ACLA_030640

NFI:	NFIA_020740

AOR:	AO090003000253 AO090003000954

ANG:	ANI_1_350014(An01g02810) ANI_1_446094(An11g03230)

AFV:	AFLA_000720 AFLA_001436

PCS:	Pc16g08330 Pc22g07520

PDP:	PDIP_21270

TMF:	PMAA_054960

TRG:	TRUGW13939_00550 TRUGW13939_08662

CIM:	CIMG_05009

CPW:	CPC735_068810

PBL:	PAAG_11073(PAAG_00230)

PBN:	PADG_07898

URE:	UREG_06546

ABE:

ARB_03814

TVE:

TRV_05093

AJE:	HCAG_06013

BGH:	BDBG_03201

PNO:	SNOG_06055

PTE:

PTT_19871

BZE:	COCCADRAFT_88203

BSC:	COCSADRAFT_221542

BOR:	COCMIDRAFT_93916

AALT:

CC77DRAFT_991604

ZTR:	MYCGRDRAFT_55438(CYP-70)

PFJ:	MYCFIDRAFT_49450

BCOM:

BAUCODRAFT_489380

NPA:	UCRNP2_2755

TML:	GSTUM_00005882001

SPO:	SPAC19A8.04(erg5)

CNE:

CNF03720

CNB:

CNBF1100

CGI:	CGB_F1500C

TMS:	TREMEDRAFT_40696

TASA:

A1Q1_00690

TVS:	TRAVEDRAFT_140384

DSQ:	DICSQDRAFT_66936

PCO:	PHACADRAFT_246788

SHS:	STEHIDRAFT_123767

HIR:	HETIRDRAFT_141009(cpm111)

PSQ:	PUNSTDRAFT_59236

ADL:	AURDEDRAFT_109660

FME:	FOMMEDRAFT_106824 FOMMEDRAFT_155371

GTR:	GLOTRDRAFT_53715

LBC:	LACBIDRAFT_187166(ERG5)

MRR:	Moror_12729

CCI:	CC1G_01671

SCM:	SCHCODRAFT_13845

ABP:	AGABI1DRAFT66440(AGABI1DRAFT_66440)

ABV:	AGABI2DRAFT189837(AGABI2DRAFT_189837)

CPUT:

CONPUDRAFT_94730

SLA:	SERLADRAFT_459032

WSE:	WALSEDRAFT_69870

WIC:	J056_001310

UMA:	UMAG_00350

PFP:	PFL1_03644

MGL:

MRT:

MSYM:

Reference

1 [PMID:8543054]

Authors

Kelly SL, Lamb DC, Corran AJ, Baldwin BC, Parks LW, Kelly DE

Title

Purification and reconstitution of activity of Saccharomyces cerevisiae P450 61, a sterol delta 22-desaturase.

Journal

FEBS Lett 377:217-20 (1995)
DOI:10.1016/0014-5793(95)01342-3

Reference

2 [PMID:8635732]

Authors

Skaggs BA, Alexander JF, Pierson CA, Schweitzer KS, Chun KT, Koegel C, Barbuch R, Bard M

Title

Cloning and characterization of the Saccharomyces cerevisiae C-22 sterol desaturase gene, encoding a second cytochrome P-450 involved in ergosterol biosynthesis.

Journal

Gene 169:105-9 (1996)
DOI:10.1016/0378-1119(95)00770-9

Sequence

[sce:YMR015C]

Reference

3 [PMID:16531502]

Authors

Morikawa T, Mizutani M, Aoki N, Watanabe B, Saga H, Saito S, Oikawa A, Suzuki H, Sakurai N, Shibata D, Wadano A, Sakata K, Ohta D.

Title

Cytochrome P450 CYP710A encodes the sterol C-22 desaturase in Arabidopsis and tomato.

Journal

Plant Cell 18:1008-22 (2006)
DOI:10.1105/tpc.105.036012