KEGG ORTHOLOGY: K09832

ORTHOLOGY: K09832

Entry

K09832                      KO

Symbol

CYP710A

Name

sterol 22-desaturase [EC:1.14.19.41]

Pathway

map00100

Steroid biosynthesis

map01100

Metabolic pathways

map01110

Biosynthesis of secondary metabolites

Reaction

R07489

beta-sitosterol,NADPH:oxygen oxidoreductase (22,23-dehydrogenating)

R11097

24-epi-campesterol,NADPH:oxygen oxidoreductase (22,23-dehydrogenating)

Brite

KEGG Orthology (KO) [BR:ko00001]
09100 Metabolism
  09103 Lipid metabolism
   00100 Steroid biosynthesis
    K09832  CYP710A; sterol 22-desaturase
09180 Brite Hierarchies
  09181 Protein families: metabolism
   00199 Cytochrome P450
    K09832  CYP710A; sterol 22-desaturase
Enzymes [BR:ko01000]
1. Oxidoreductases
  1.14  Acting on paired donors, with incorporation or reduction of molecular oxygen
   1.14.19  With oxidation of a pair of donors resulting in the reduction of O2 to two molecules of water
    1.14.19.41  sterol 22-desaturase
     K09832  CYP710A; sterol 22-desaturase
Cytochrome P450 [BR:ko00199]
Cytochrome P450, plant type
  CYP710 family
   K09832  CYP710A; sterol 22-desaturase

Other DBs

COG:

COG2124

GO:

0000249

Genes

ATH:	AT2G28850(CYP710A3) AT2G28860(CYP710A4) AT2G34490(CYP710A2) AT2G34500(CYP710A1)

ALY:	9317081 9317405 9317406

CRB:	17887986 17889424 17889482

CSAT:

104699232 104699233 104700630 104750004 104750006 104753438 104781842 104781843 104786110 104786111 104786814 104789238 104789239 104789240 104792202 104792203

EUS:	EUTSA_v10016547mg EUTSA_v10016552mg

BRP:	103865343 103867449

BNA:	106432759 106447495 125585615 125608302 125609361

BOE:	106339100 106339917 106340751 106341405

RSZ:	108807244 108852806

THJ:

104814293

CPAP:

110807637

CIT:

102618952

CIC:	CICLE_v10014973mg

PVY:

116141721

MINC:

123192456 123218316

TCC:

18596516

GRA:	105769297 105769298 105772027 105774040

GHI:	107901888 107905877 107905878 107942637 121203644

GAB:	108452825 108452842

HSYR:

120131393 120190641 120194923

DZI:	111280976 111318668

EGR:

104440796

RARG:

115731219 125316705

GMX:	100787616 100808148

GSJ:	114377625 114387884

PVU:

VRA:

VAR:

VUN:

VUM:

CCAJ:

APRC:

MTR:

TPRA:

CAM:

PSAT:

VVO:

LJA:

ADU:

AIP:

AHF:	112714308 112782610

LANG:

109328906 109329075 109345270

PCIN:

129286005

QSA:	O6P43_012958

FVE:

RCN:

PPER:

PMUM:

PAVI:

PDUL:

MDM:

MSYL:

PCOX:

ZJU:

MNT:

CSV:

CMO:

BHJ:

CMAX:

CMOS:

CPEP:

MEAN:

126653830 126680212 126683299

HBR:	110640583 110645097

MESC:

POP:

PEU:

PALZ:

PNZ:

JRE:

CILL:

CAVE:

AGLU:

QSU:

QLO:	115980600 115992293

TWL:

119995149

VVI:

100255505

VRI:

117922864

SLY:	100136886(CYP710A11)

SPEN:

SOT:

SSTN:

SDUL:

CANN:

LBB:

NTA:	107763057 107771426(SD1)

NSY:

NTO:

NAU:

INI:

ITR:

SIND:

OEU:	111407278 111412227

EGT:

SSPL:

SMIL:

SHIS:

APAN:

HPUM:

PHUA:

140965279 140979025 140984158

PEBU:

140815321 140836721 140838696

HAN:	110911670 110937398

ECAD:

LSV:

CCAV:

DCR:

CSIN:

RVL:	131312423 131312424 131312426

AEW:

130755298

DLT:

127793255

BVG:

104883528

SOE:	110778797 110778903

CQI:	110730201 110732522

ATRI:

MOF:

NNU:

MING:

TSS:	122639679 122650136

PSOM:

113329902 113338741 113345304 113355287

OSA:	4325562 4325563 4325564 9267882

DOSA:

Os01g0210900 Os01g0211000 Os01g0211200 Os01g0211600

OBR:	102709230 102711741

OGL:	127759857 127760109 127760169 127760847

BDI:

100827562

ATS:	109757150 109772439

TDC:	119267541 119275444 119292328 119298617 119304413 119338528 119338529 119343557 119344120

TAES:

123060655 123069249 123077753 123094069 123099277 123157569 123162932

TUA:	125507657 125543216

LPER:

127294839 127321331 127323133

LRD:	124647277 124658988 124663956 124663963 124663967 124664979 124667667 124668114 124695192 124695193

SBI:

8079522

ZMA:

107305676

MFLO:

136451400 136455075 136457455

SITA:

101764868 101770311

SVS:	117837101 117858509

PVIR:

120676345 120705614

PHAI:

112893543

PAUA:

133891535 133893054 133919677

TANG:

140769333 140778633

PDA:	103702545 103703021

EGU:	105040643 105048162

MUS:	103995072 135624854

ZOF:	121989158 121998106 122052204 122056687

DCT:

PEQ:

AOF:

MSIN:

ACAK:

NCOL:

ATR:

CJF:

SMO:	SELMODRAFT_234389 SELMODRAFT_75218(CYP710A21)

PPP:	112277322 112285903

» show all

Reference

PMID:16531502 (CYP710A1 CYP710A2)

Authors

Morikawa T, Mizutani M, Aoki N, Watanabe B, Saga H, Saito S, Oikawa A, Suzuki H, Sakurai N, Shibata D, Wadano A, Sakata K, Ohta D.

Title

Cytochrome P450 CYP710A encodes the sterol C-22 desaturase in Arabidopsis and tomato.

Journal

Plant Cell 18:1008-22 (2006)
DOI:10.1105/tpc.105.036012

Sequence

[ath:AT2G34500 AT2G34490]

LinkDB

ENZYME: 1.14.19.41

Entry

EC 1.14.19.41               Enzyme

Name

sterol 22-desaturase;
ERG5 (gene name);
CYP710A (gene name)

Class

Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With oxidation of a pair of donors resulting in the reduction of O2 to two molecules of water

Sysname

ergosta-5,7,24(28)-trien-3beta-ol,NADPH:oxygen oxidoreductase (22,23-dehydrogenating)

Reaction(IUBMB)

ergosta-5,7,24(28)-trien-3beta-ol + NADPH + H+ + O2 = ergosta-5,7,22,24(28)-tetraen-3beta-ol + NADP+ + 2 H2O [RN:R07506]

Reaction(KEGG)

R07506;
(other) R07489 R11097

Substrate

ergosta-5,7,24(28)-trien-3beta-ol [CPD:C15780];
NADPH [CPD:C00005];
H+ [CPD:C00080];
O2 [CPD:C00007]

Product

ergosta-5,7,22,24(28)-tetraen-3beta-ol [CPD:C05440];
NADP+ [CPD:C00006];
H2O [CPD:C00001]

Comment

A heme-thiolate protein (P-450). The enzyme, found in yeast and plants, catalyses the introduction of a double bond between the C-22 and C-23 carbons of certain sterols. In yeast the enzyme acts on ergosta-5,7,24(28)-trien-3beta-ol, a step in the biosynthesis of ergosterol. The enzyme from the plant Arabidopsis thaliana acts on sitosterol and 24-epi-campesterol, producing stigmasterol and brassicasterol, respectively.

History

EC 1.14.19.41 created 2015

Pathway

ec00100

Steroid biosynthesis

ec01100

Metabolic pathways

ec01110

Biosynthesis of secondary metabolites

Orthology

K09831

sterol 22-desaturase

K09832

sterol 22-desaturase

Genes

ATH:	AT2G28850(CYP710A3) AT2G28860(CYP710A4) AT2G34490(CYP710A2) AT2G34500(CYP710A1)

ALY:	9317081 9317405 9317406

CRB:	17887986 17889424 17889482

CSAT:

104699232 104699233 104700630 104750004 104750006 104753438 104781842 104781843 104786110 104786111 104786814 104789238 104789239 104789240 104792202 104792203

EUS:	EUTSA_v10016547mg EUTSA_v10016552mg

BRP:	103865343 103867449

BNA:	106432759 106447495 125585615 125608302 125609361

BOE:	106339100 106339917 106340751 106341405

RSZ:	108807244 108852806

THJ:

104814293

CPAP:

110807637

CIT:

102618952

CIC:	CICLE_v10014973mg

PVY:

116141721

MINC:

123192456 123218316

TCC:

18596516

GRA:	105769297 105769298 105772027 105774040

GHI:	107901888 107905877 107905878 107942637 121203644

GAB:	108452825 108452842

HSYR:

120131393 120190641 120194923

DZI:	111280976 111318668

EGR:

104440796

RARG:

115731219 125316705

GMX:	100787616 100808148

GSJ:	114377625 114387884

PVU:

137832360

VRA:

106776160

VAR:

108340983

VUN:

114188144

VUM:

124834997

CCAJ:

109789198

APRC:

113852140

MTR:

11437817

TPRA:

123898150 123905820

CAM:

101489453

PSAT:

127079474

VVO:

131595535

LJA:

130724467

ADU:

107468177

AIP:

107623676

AHF:	112714308 112782610

LANG:

109328906 109329075 109345270

PCIN:

129286005

QSA:	O6P43_012958

FVE:

105350241

RCN:

112166774

PPER:

18778941

PMUM:

103325114

PAVI:

110749808 110773983

PDUL:

117625864

MDM:

103434989

MSYL:

126621236

PCOX:

137721515

ZJU:

107413371

MNT:

21404536

CSV:

101221194

CMO:

103484375

BHJ:

120074625

CMAX:

111478743 111496674

CMOS:

111433344 111433531

CPEP:

111782177 111803380

MEAN:

126653830 126680212 126683299

HBR:	110640583 110645097

MESC:

110609904 110621253

POP:

7472572

PEU:

105117943

PALZ:

118050840

PNZ:

133699862

JRE:

109021374

CILL:

122275110

CAVE:

132171142

AGLU:

133867636 133869533

QSU:

112009839

QLO:	115980600 115992293

TWL:

119995149

VVI:

100255505

VRI:

117922864

SLY:	100136886(CYP710A11)

SPEN:

107011895

SOT:

102581211

SSTN:

125848721

SDUL:

129874504

CANN:

107859112

LBB:

132623336

NTA:	107763057 107771426(SD1)

NSY:

104234654

NTO:

104096813

NAU:

109208410

INI:

109192033

ITR:

116017561

SIND:

105167297 105172499

OEU:	111407278 111412227

EGT:

105951371

SSPL:

121805298 121808148

SMIL:

131013605

SHIS:

125194367

APAN:

127254813

HPUM:

140863114 140891401

PHUA:

140965279 140979025 140984158

PEBU:

140815321 140836721 140838696

HAN:	110911670 110937398

ECAD:

122593276

LSV:

111890946

CCAV:

112504656

DCR:

108199974

CSIN:

114313482

RVL:	131312423 131312424 131312426

AEW:

130755298

DLT:

127793255

BVG:

104883528

SOE:	110778797 110778903

CQI:	110730201 110732522

ATRI:

130814489 130823996

MOF:

131144302

NNU:

104597733

MING:

122060078 122092751

TSS:	122639679 122650136

PSOM:

113329902 113338741 113345304 113355287

OSA:	4325562 4325563 4325564 9267882

DOSA:

Os01g0210900 Os01g0211000 Os01g0211200 Os01g0211600

OBR:	102709230 102711741

OGL:	127759857 127760109 127760169 127760847

BDI:

100827562

ATS:	109757150 109772439

TDC:	119267541 119275444 119292328 119298617 119304413 119338528 119338529 119343557 119344120

TAES:

123060655 123069249 123077753 123094069 123099277 123157569 123162932

TUA:	125507657 125543216

LPER:

127294839 127321331 127323133

LRD:	124647277 124658988 124663956 124663963 124663967 124664979 124667667 124668114 124695192 124695193

SBI:

8079522

ZMA:

107305676

MFLO:

136451400 136455075 136457455

SITA:

101764868 101770311

SVS:	117837101 117858509

PVIR:

120676345 120705614

PHAI:

112893543

PAUA:

133891535 133893054 133919677

TANG:

140769333 140778633

PDA:	103702545 103703021

EGU:	105040643 105048162

MUS:	103995072 135624854

ZOF:	121989158 121998106 122052204 122056687

DCT:

110110071

PEQ:

110029296

AOF:

109828814

MSIN:

131245570

ACAK:

137934224

NCOL:

116265070

ATR:

18435199

CJF:

131044975

SMO:	SELMODRAFT_234389 SELMODRAFT_75218(CYP710A21)

PPP:	112277322 112285903

SCE:	YMR015C(ERG5)

SEUB:

DI49_4102

SPAO:

SPAR_M01390

AGO:	AGOS_ABR053C

KLA:	KLLA0_D11242g

KMX:	KLMA_60417(cyp524A1)

LTH:	KLTH0D07832g

VPO:	Kpol_1056p10

ZRO:	ZYRO0E06204g

CGR:	2891731(ERG5)

NCS:	NCAS_0A12700(NCAS0A12700)

NDI:	NDAI_0B01560(NDAI0B01560)

TPF:	TPHA_0I02420(TPHA0I02420)

TBL:	TBLA_0H02650(TBLA0H02650)

TGB:	HG536_0G01250

TDL:	TDEL_0B06510(TDEL0B06510)

KAF:	KAFR_0A02110(KAFR0A02110)

KNG:	KNAG_0M02100(KNAG0M02100)

ZMK:	HG535_0G03560

SLUD:

SCDLUD_004216

DHA:	DEHA2D06182g

PIC:	PICST_73043(ERG5)

PGU:	PGUG_05085

SPAA:

SPAPADRAFT_63544(ERG5)

LEL:	PVL30_005321(ERG5)

LBG:	92211304(LODBEIA_P61080)

CAL:	CAALFM_C702840CA(ERG5)

CTP:	CTRG_04983

COT:	CORT_0G04140

CDU:	CD36_72510

CTEN:

18247844(ERG5)

CLU:	CLUG_04246

CLUS:

A9F13_04g02805

CAUR:

CJI96_0004850 CJI96_0004910

ASAU:

88173733(PUMCH_002669)

PPA:	PAS_chr3_0507

PKZ:	C5L36_0B12520

BNN:	FOA43_004793(ERG5)

BBRX:

BRETT_002777(ERG5)

OPA:	HPODL_01958

YLI:	2906079(YALI2_A00419g)

SLB:	AWJ20_75(ERG5)

NCR:

NCU05278

NTE:	NEUTE1DRAFT116692(NEUTE1DRAFT_116692)

SMP:	10806154(SMAC4_01710)

PAN:	PODANSg6262 PODANSg7995

PBEL:

QC761_202070 QC761_304420(ERG5)

PPSD:

QC762_202070 QC762_304420(ERG5)

PPSP:

QC763_202070 QC763_304420(ERG5)

PPSA:

QC764_202070 QC764_304420(ERG5)

TTT:	THITE_128412

MTM:	MYCTH_73679

CTHR:

CTHT_0029760

RTHE:

98125952(VTJ83DRAFT_4781)

MGR:

MGG_06047

PPEI:

PpBr36_02869

PGRI:

PgNI_01835

TMN:	UCRPA7_7946

SSCK:

SPSK_00476

FGR:	FGSG_01959 FGSG_03686

FPU:	FPSE_01847 FPSE_09181

FPOA:

FPOAC1_002075 FPOAC1_006089

FVN:	FVRRES_02319 FVRRES_06686

FVR:	FVEG_07284 FVEG_08786

FOX:	FOXG_04166 FOXG_09685

NHE:	NECHADRAFT_72313 NECHADRAFT_72652

FFC:	NCS54_01269600 NCS54_01305200

FKR:	NCS57_01239900 NCS57_01291900

FMU:	J7337_010705 J7337_012541

TRE:	TRIREDRAFT_122166 TRIREDRAFT_58689

TRR:	M419DRAFT_141218 M419DRAFT_74177

TATV:

25781109(TrAtP1_001090) 25784525(TrAtP1_005351)

TASP:

36607958(TrAFT101_001364) 36618431(TrAFT101_011828)

MAW:	19252947(ERG5_1)

MAJ:

MAA_01109

MBRN:

26239210(ERG5)

PCHM:

VFPPC_03546

CMT:

CCM_02962

AMUS:

LMH87_000586 LMH87_006973

PLJ:	28882794(PLICBS_001203) 28890467(PLICBS_009795)

PTKZ:

JDV02_004750 JDV02_007982(ERG5)

VAL:	VDBG_00192 VDBG_00755

VDA:	VDAG_00367 VDAG_08476

CFJ:	CFIO01_05732 CFIO01_13734

CLUP:

CLUP02_04776 CLUP02_14903

CHIG:

CH63R_04552 CH63R_14243

CDET:

87941614(CDEST_05111) 87949434(CDEST_12934)

SAPO:

SAPIO_CDS4161 SAPIO_CDS7005

ELA:	UCREL1_11694

PFY:	PFICI_04403 PFICI_13034

SSL:	SS1G_09832

BFU:	BCIN_07g01120(Bcerg5)

MBE:

MBM_06688

PSCO:

LY89DRAFT_257563 LY89DRAFT_584410

GLZ:	GLAREA_02023 GLAREA_06590

ANI:	ANIA_04042

AFM:	AFUA_1G03950

ACT:	ACLA_008020 ACLA_030640

NFI:	NFIA_020740

AOR:	AO090003000253 AO090003000954

ANG:	An01g02810 An11g03230

AFV:	AFLA_000720 AFLA_001436

ALUC:

AKAW2_20450S(ERG5_1) AKAW2_80298S(ERG5_2)

ACHE:

ACHE_11570S(ERG5)

APUU:

APUU_40308A(ERG5) APUU_60358A

PCS:	N7525_005484 N7525_010907

PDP:	PDIP_21270

POU:	POX_e06874

TMF:	EYB26_008188

TRG:	TRUGW13939_00550 TRUGW13939_08662

CIM:	CIMG_05009

CPW:	9696838(ERG5)

URE:	UREG_06546

ABE:

ARB_03814

TVE:

TRV_05093

PBL:	PAAG_11073(PAAG_00230)

PBN:	PADG_07898

AJE:	HCAG_06013

BGH:	BDBG_03201

PNO:	SNOG_06055

PTE:

PTT_19871

PTRR:

6343165(PtrM4_119060)

BZE:	COCCADRAFT_88203

BSC:	COCSADRAFT_221542

BOR:	COCMIDRAFT_93916

AALT:

CC77DRAFT_991604

ADAC:

96086125(ACET3X_005803)

ARAB:

EKO05_0000970

ZTR:	MYCGRDRAFT_55438(CYP-70)

PFJ:	MYCFIDRAFT_49450

FFU:	CLAFUR5_02386

CBET:

CB0940_03149

BCOM:

BAUCODRAFT_489380

NPA:	UCRNP2_2755

TML:	GSTUM_00005882001

SPO:	2542469(erg5)

SOM:	SOMG_03716(erg5)

CNE:

CNF03720

CNB:

CNBF1100

CNG:	CNAG_06644

CGI:	CGB_F1500C

CDEU:

CNBG_1921

CDEC:

89990868(IAS62_004096)

CTEG:

91990496(I308_103640)

CBAS:

91984092(I312_104047)

CDEP:

91090557(L203_106349)

KMG:	30160844(I203_105212)

KNE:	92184306(IAR55_007048)

KDJ:	28972325(I303_106731)

KBI:	30208979(I302_101207)

KPIN:

30173683(I206_107142)

KSH:	87958078(IL334_005948)

KER:	91103540(V865_004739)

TMS:	TREMEDRAFT_40696

TASA:

A1Q1_00690

CCAC:

CcaHIS019_0405230(ERG5)

TVS:	TRAVEDRAFT_140384

DSQ:	DICSQDRAFT_66936

PCO:	PHACADRAFT_246788

SHS:	STEHIDRAFT_123767

HIR:	HETIRDRAFT_141009(cpm111)

PSQ:	PUNSTDRAFT_59236

ADL:	AURDEDRAFT_109660

FME:	FOMMEDRAFT_106824 FOMMEDRAFT_155371

GTR:	GLOTRDRAFT_53715

RSX:	RhiXN_01858

LBC:	LACBIDRAFT_187166(ERG5)

CCI:	CC1G_01671

ABP:	AGABI1DRAFT66440(AGABI1DRAFT_66440)

ABV:	AGABI2DRAFT189837(AGABI2DRAFT_189837)

PCUB:

JR316_0000711

MRR:	Moror_12729

E1B28_009893

SCM:	SCHCO_02609502(SCHCODRAFT_02609502)

CPUT:

CONPUDRAFT_94730

SLA:	SERLADRAFT_459032

WSE:	WALSEDRAFT_69870

WIC:	J056_001310

UMA:	UMAG_00350

PFP:	PFL1_03644

SGRA:

EX895_000577

MGL:

MGL_0310

MRT:

MRET_0994

MSYM:

MSY001_2710

» show all

Reference

1 [PMID:8543054]

Authors

Kelly SL, Lamb DC, Corran AJ, Baldwin BC, Parks LW, Kelly DE

Title

Purification and reconstitution of activity of Saccharomyces cerevisiae P450 61, a sterol delta 22-desaturase.

Journal

FEBS Lett 377:217-20 (1995)
DOI:10.1016/0014-5793(95)01342-3

Reference

2 [PMID:8635732]

Authors

Skaggs BA, Alexander JF, Pierson CA, Schweitzer KS, Chun KT, Koegel C, Barbuch R, Bard M

Title

Cloning and characterization of the Saccharomyces cerevisiae C-22 sterol desaturase gene, encoding a second cytochrome P-450 involved in ergosterol biosynthesis.

Journal

Gene 169:105-9 (1996)
DOI:10.1016/0378-1119(95)00770-9

Sequence

[sce:YMR015C]

Reference

3 [PMID:16531502]

Authors

Morikawa T, Mizutani M, Aoki N, Watanabe B, Saga H, Saito S, Oikawa A, Suzuki H, Sakurai N, Shibata D, Wadano A, Sakata K, Ohta D.

Title

Cytochrome P450 CYP710A encodes the sterol C-22 desaturase in Arabidopsis and tomato.

Journal

Plant Cell 18:1008-22 (2006)
DOI:10.1105/tpc.105.036012

Sequence

[ath:AT2G34500 AT2G34490]

Other DBs

ExplorEnz - The Enzyme Database:

1.14.19.41

IUBMB Enzyme Nomenclature:

1.14.19.41

ExPASy - ENZYME nomenclature database:

1.14.19.41

BRENDA, the Enzyme Database:

1.14.19.41

LinkDB

REACTION: R07489

Entry

R07489                      Reaction

Name

beta-sitosterol,NADPH:oxygen oxidoreductase (22,23-dehydrogenating)

Definition

beta-Sitosterol + NADPH + H+ + Oxygen <=> Stigmasterol + NADP+ + 2 H2O

Equation

C01753 + C00005 + C00080 + C00007 <=> C05442 + C00006 + 2 C00001

Reaction class

RC00001

C00005_C00006

RC01886

C01753_C05442

Enzyme

1.14.19.41

Pathway

rn00100

Steroid biosynthesis

rn01100

Metabolic pathways

rn01110

Biosynthesis of secondary metabolites

Brite

Enzymatic reactions [BR:br08201]
1. Oxidoreductase reactions
  1.14  Acting on paired donors, with incorporation or reduction of molecular oxygen
   1.14.19  With oxidation of a pair of donors resulting in the reduction of O2 to two molecules of water
    1.14.19.41
     R07489  beta-Sitosterol + NADPH + H+ + Oxygen <=> Stigmasterol + NADP+ + 2 H2O

Orthology

K09832

sterol 22-desaturase [EC:1.14.19.41]

Other DBs

RHEA:

46579

LinkDB

DBGET integrated database retrieval system