KEGG   ORTHOLOGY: K09832
Entry
K09832                      KO                                     
Symbol
CYP710A
Name
sterol 22-desaturase [EC:1.14.19.41]
Pathway
map00100  Steroid biosynthesis
map01100  Metabolic pathways
map01110  Biosynthesis of secondary metabolites
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09103 Lipid metabolism
   00100 Steroid biosynthesis
    K09832  CYP710A; sterol 22-desaturase
 09180 Brite Hierarchies
  09181 Protein families: metabolism
   00199 Cytochrome P450
    K09832  CYP710A; sterol 22-desaturase
Enzymes [BR:ko01000]
 1. Oxidoreductases
  1.14  Acting on paired donors, with incorporation or reduction of molecular oxygen
   1.14.19  With oxidation of a pair of donors resulting in the reduction of O2 to two molecules of water
    1.14.19.41  sterol 22-desaturase
     K09832  CYP710A; sterol 22-desaturase
Cytochrome P450 [BR:ko00199]
 Cytochrome P450, plant type
  CYP710 family
   K09832  CYP710A; sterol 22-desaturase
Other DBs
RN: R07489 R11097
COG: COG2124
GO: 0000249
Genes
ATH: AT2G28850(CYP710A3) AT2G28860(CYP710A4) AT2G34490(CYP710A2) AT2G34500(CYP710A1)
ALY: 9317081 9317405 9317406
CRB: 17887986 17889424 17889482
CSAT: 104699232 104699233 104700630 104750004 104750006 104753438 104781842 104781843 104786110 104786111 104786814 104789238 104789239 104789240 104792202 104792203
EUS: EUTSA_v10016547mg EUTSA_v10016552mg
BRP: 103865343 103867449
BNA: 106396203 106432759 106432788 106447495
BOE: 106339100 106339917 106340751 106341405
RSZ: 108807244 108833237 108852806
THJ: 104814293
CPAP: 110807637
CIT: 102618952
CIC: CICLE_v10014973mg
PVY: 116141721
MINC: 123192456 123218316
TCC: 18596516
GRA: 105769297 105769298 105772027 105774040
GHI: 107901888 107905877 107905878 107942637 121203644
GAB: 108452825 108452842
DZI: 111280976 111318668
EGR: 104440796
GMX: 100787616 100808148
GSJ: 114377625 114387884
PVU: PHAVU_006G163800g
VRA: 106776160
VAR: 108340983
VUN: 114188144
CCAJ: 109789198
APRC: 113852140
MTR: 11437817
CAM: 101489453
ADU: 107468177
AIP: 107623676
AHF: 112714308 112782610
LANG: 109328906 109329075 109345270
FVE: 105350241
RCN: 112166774
PPER: 18778941
PMUM: 103325114
PAVI: 110749808 110773983
PDUL: 117625864
MDM: 103434989
ZJU: 107413371
MNT: 21404536
CSV: 101221194
CMO: 103484375
BHJ: 120074625
CMAX: 111478743 111496674
CMOS: 111433344 111433531
CPEP: 111782177 111803380
HBR: 110640583 110645097
MESC: 110609904 110621253
POP: 7472572
PEU: 105117943
PALZ: 118050840
JRE: 109021374
QSU: 112009839
QLO: 115980600 115992293
TWL: 119995149
VVI: 100255505
VRI: 117922864
SLY: 100136886(CYP710A11)
SPEN: 107011895
SOT: 102581211
SSTN: 125848721
CANN: 107859112
NTA: 107763057 107771426(SD1)
NSY: 104234654
NTO: 104096813
NAU: 109208410
INI: 109192033
ITR: 116017561
SIND: 105167297 105172499
OEU: 111407278 111412227
EGT: 105951371
SSPL: 121805298 121808148
HAN: 110911670 110937398
ECAD: 122593276
LSV: 111890946
CCAV: 112504656
DCR: 108199974
CSIN: 114313482
BVG: 104883528
SOE: 110778797 110778903
CQI: 110730201 110732522
NNU: 104597733
MING: 122060078 122092751
TSS: 122639679 122650136
PSOM: 113329902 113338741 113345304 113355287
NCOL: 116265070
OSA: 4325562 4325563 4325564 9267882
DOSA: Os01t0210900-01(Os01g0210900) Os01t0211000-00(Os01g0211000) Os01t0211200-00(Os01g0211200) Os01t0211600-01(Os01g0211600)
OBR: 102709230 102711741
BDI: 100827562
ATS: 109746138 109757150 109772439
TDC: 119267541 119275444 119292328 119298617 119304413 119338528 119338529 119343557 119344120
TAES: 123060655 123069249 123077753 123094069 123099277 123157569 123162932
TUA: 125507657 125543216
SBI: 8079522
ZMA: 107305676
SITA: 101764868 101770311
SVS: 117837101 117858509
PVIR: 120676345 120705614
PHAI: 112893543
PDA: 103702545 103703021
EGU: 105040643 105048162
MUS: 103995072 103999677 104000847
DCT: 110110071
PEQ: 110029296
AOF: 109828814
ATR: 18435199
SMO: SELMODRAFT_234389 SELMODRAFT_75218(CYP710A21)
PPP: 112277322 112285903
 » show all
Reference
PMID:16531502 (CYP710A1 CYP710A2)
  Authors
Morikawa T, Mizutani M, Aoki N, Watanabe B, Saga H, Saito S, Oikawa A, Suzuki H, Sakurai N, Shibata D, Wadano A, Sakata K, Ohta D.
  Title
Cytochrome P450 CYP710A encodes the sterol C-22 desaturase in Arabidopsis and tomato.
  Journal
Plant Cell 18:1008-22 (2006)
DOI:10.1105/tpc.105.036012
  Sequence
[ath:AT2G34500 AT2G34490]
LinkDB

KEGG   ENZYME: 1.14.19.41
Entry
EC 1.14.19.41               Enzyme                                 
Name
sterol 22-desaturase;
ERG5 (gene name);
CYP710A (gene name)
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With oxidation of a pair of donors resulting in the reduction of O2 to two molecules of water
Sysname
ergosta-5,7,24(28)-trien-3beta-ol,NADPH:oxygen oxidoreductase (22,23-dehydrogenating)
Reaction(IUBMB)
ergosta-5,7,24(28)-trien-3beta-ol + NADPH + H+ + O2 = ergosta-5,7,22,24(28)-tetraen-3beta-ol + NADP+ + 2 H2O [RN:R07506]
Reaction(KEGG)
R07506;
(other) R07489 R11097
Substrate
ergosta-5,7,24(28)-trien-3beta-ol [CPD:C15780];
NADPH [CPD:C00005];
H+ [CPD:C00080];
O2 [CPD:C00007]
Product
ergosta-5,7,22,24(28)-tetraen-3beta-ol [CPD:C05440];
NADP+ [CPD:C00006];
H2O [CPD:C00001]
Comment
A heme-thiolate protein (P-450). The enzyme, found in yeast and plants, catalyses the introduction of a double bond between the C-22 and C-23 carbons of certain sterols. In yeast the enzyme acts on ergosta-5,7,24(28)-trien-3beta-ol, a step in the biosynthesis of ergosterol. The enzyme from the plant Arabidopsis thaliana acts on sitosterol and 24-epi-campesterol, producing stigmasterol and brassicasterol, respectively.
History
EC 1.14.19.41 created 2015
Pathway
ec00100  Steroid biosynthesis
ec01100  Metabolic pathways
ec01110  Biosynthesis of secondary metabolites
Orthology
K09831  sterol 22-desaturase
K09832  sterol 22-desaturase
Genes
ATH: AT2G28850(CYP710A3) AT2G28860(CYP710A4) AT2G34490(CYP710A2) AT2G34500(CYP710A1)
ALY: 9317081 9317405 9317406
CRB: 17887986 17889424 17889482
CSAT: 104699232 104699233 104700630 104750004 104750006 104753438 104781842 104781843 104786110 104786111 104786814 104789238 104789239 104789240 104792202 104792203
EUS: EUTSA_v10016547mg EUTSA_v10016552mg
BRP: 103865343 103867449
BNA: 106396203 106432759 106432788 106447495
BOE: 106339100 106339917 106340751 106341405
RSZ: 108807244 108833237 108852806
THJ: 104814293
CPAP: 110807637
CIT: 102618952
CIC: CICLE_v10014973mg
PVY: 116141721
MINC: 123192456 123218316
TCC: 18596516
GRA: 105769297 105769298 105772027 105774040
GHI: 107901888 107905877 107905878 107942637 121203644
GAB: 108452825 108452842
DZI: 111280976 111318668
EGR: 104440796
GMX: 100787616 100808148
GSJ: 114377625 114387884
PVU: PHAVU_006G163800g
VRA: 106776160
VAR: 108340983
VUN: 114188144
CCAJ: 109789198
APRC: 113852140
MTR: 11437817
CAM: 101489453
ADU: 107468177
AIP: 107623676
AHF: 112714308 112782610
LANG: 109328906 109329075 109345270
FVE: 105350241
RCN: 112166774
PPER: 18778941
PMUM: 103325114
PAVI: 110749808 110773983
PDUL: 117625864
MDM: 103434989
ZJU: 107413371
MNT: 21404536
CSV: 101221194
CMO: 103484375
BHJ: 120074625
CMAX: 111478743 111496674
CMOS: 111433344 111433531
CPEP: 111782177 111803380
HBR: 110640583 110645097
MESC: 110609904 110621253
POP: 7472572
PEU: 105117943
PALZ: 118050840
JRE: 109021374
QSU: 112004265 112009839 112037010
QLO: 115980600 115992293
TWL: 119995149
VVI: 100255505
VRI: 117922864
SLY: 100136886(CYP710A11)
SPEN: 107011895
SOT: 102581211
SSTN: 125848721
CANN: 107859112
NTA: 107763057 107771426(SD1)
NSY: 104234654
NTO: 104096813
NAU: 109208410
INI: 109192033
ITR: 116017561
SIND: 105167297 105172499
OEU: 111407278 111412227
EGT: 105951371
SSPL: 121805298 121808148
HAN: 110911670 110937398
ECAD: 122593276
LSV: 111890946
CCAV: 112504656
DCR: 108199974
CSIN: 114313482
BVG: 104883528
SOE: 110778797 110778903
CQI: 110730201 110732522
NNU: 104597733
MING: 122060078 122092751
TSS: 122639679 122650136
PSOM: 113329902 113338741 113345304 113355287
NCOL: 116265070
OSA: 4325562 4325563 4325564 9267882
DOSA: Os01t0210900-01(Os01g0210900) Os01t0211000-00(Os01g0211000) Os01t0211200-00(Os01g0211200) Os01t0211600-01(Os01g0211600)
OBR: 102709230 102711741
BDI: 100827562
ATS: 109746138 109757150 109772439
TDC: 119267541 119275444 119292328 119298617 119304413 119338528 119338529 119343557 119344120
TAES: 123060655 123069249 123077753 123094069 123099277 123157569 123162932
TUA: 125507657 125543216
SBI: 8079522
ZMA: 107305676
SITA: 101764868 101770311
SVS: 117837101 117858509
PVIR: 120676345 120705614
PHAI: 112893543
PDA: 103702545 103703021
EGU: 105040643 105048162
MUS: 103995072 103999677 104000847
DCT: 110110071
PEQ: 110029296
AOF: 109828814
ATR: 18435199
SMO: SELMODRAFT_234389 SELMODRAFT_75218(CYP710A21)
PPP: 112277322 112285903
SCE: YMR015C(ERG5)
SEUB: DI49_4102
SPAO: SPAR_M01390
AGO: AGOS_ABR053C
KLA: KLLA0_D11242g
KMX: KLMA_60417(cyp524A1)
LTH: KLTH0D07832g
VPO: Kpol_1056p10
ZRO: ZYRO0E06204g
CGR: CAGL0M07656g
NCS: NCAS_0A12700(NCAS0A12700)
NDI: NDAI_0B01560(NDAI0B01560)
TPF: TPHA_0I02420(TPHA0I02420)
TBL: TBLA_0H02650(TBLA0H02650)
TDL: TDEL_0B06510(TDEL0B06510)
TGB: HG536_0G01250
KAF: KAFR_0A02110(KAFR0A02110)
KNG: KNAG_0M02100(KNAG0M02100)
ZMK: HG535_0G03560
PPA: PAS_chr3_0507
DHA: DEHA2D06182g
PIC: PICST_73043(ERG5)
PGU: PGUG_05085
SPAA: SPAPADRAFT_63544(ERG5)
LEL: LELG_05188
CAL: CAALFM_C702840CA(ERG5)
CTP: CTRG_04983
COT: CORT_0G04140
CDU: CD36_72510
CTEN: CANTEDRAFT_115386
YLI: YALI0A18062g
CLU: CLUG_04246
CLUS: A9F13_04g02805
CAUR: CJI97_001893
SLB: AWJ20_75(ERG5)
PKZ: C5L36_0B12520
BNN: FOA43_004793(ERG5)
BBRX: BRETT_002777(ERG5)
OPA: HPODL_01958
SLUD: SCDLUD_004216
NCR: NCU05278
NTE: NEUTE1DRAFT116692(NEUTE1DRAFT_116692)
SMP: SMAC_01710
PAN: PODANSg6262 PODANSg7995
TTT: THITE_128412
MTM: MYCTH_73679
CTHR: CTHT_0029760
MGR: MGG_06047
PPEI: PpBr36_02869
TMN: UCRPA7_7946
SSCK: SPSK_00476
FGR: FGSG_01959 FGSG_03686
FPU: FPSE_01847 FPSE_09181
FVR: FVEG_07284 FVEG_08786
FOX: FOXG_04166 FOXG_09685
NHE: NECHADRAFT_72313 NECHADRAFT_72652
TRE: TRIREDRAFT_122166 TRIREDRAFT_58689
TRR: M419DRAFT_141218 M419DRAFT_74177
MAW: MAC_08636
MAJ: MAA_01109
CMT: CCM_02962
PLJ: VFPFJ_00660 VFPFJ_08344
VAL: VDBG_00192 VDBG_00755
VDA: VDAG_00367 VDAG_08476
CFJ: CFIO01_05732 CFIO01_13734
CLUP: CLUP02_04776 CLUP02_14903
CHIG: CH63R_04552 CH63R_14243
SAPO: SAPIO_CDS4161 SAPIO_CDS7005
ELA: UCREL1_11694
PFY: PFICI_04403 PFICI_13034
SSL: SS1G_09832
BFU: BCIN_07g01120(Bcerg5)
MBE: MBM_06688
PSCO: LY89DRAFT_257563 LY89DRAFT_584410
GLZ: GLAREA_02023 GLAREA_06590
ANI: AN4042.2
AFM: AFUA_1G03950
ACT: ACLA_008020 ACLA_030640
NFI: NFIA_020740
AOR: AO090003000253 AO090003000954
ANG: ANI_1_350014(An01g02810) ANI_1_446094(An11g03230)
AFV: AFLA_000720 AFLA_001436
ALUC: AKAW2_20450S(ERG5_1) AKAW2_80298S(ERG5_2)
ACHE: ACHE_11570S(ERG5)
APUU: APUU_40308A(ERG5) APUU_60358A
PCS: Pc16g08330 Pc22g07520
PDP: PDIP_21270
TMF: PMAA_054960
TRG: TRUGW13939_00550 TRUGW13939_08662
CIM: CIMG_05009
CPW: CPC735_068810
URE: UREG_06546
PBL: PAAG_11073(PAAG_00230)
PBN: PADG_07898
ABE: ARB_03814
TVE: TRV_05093
AJE: HCAG_06013
BGH: BDBG_03201
PNO: SNOG_06055
PTE: PTT_19871
BZE: COCCADRAFT_88203
BSC: COCSADRAFT_221542
BOR: COCMIDRAFT_93916
AALT: CC77DRAFT_991604
ZTR: MYCGRDRAFT_55438(CYP-70)
PFJ: MYCFIDRAFT_49450
FFU: CLAFUR5_02386
BCOM: BAUCODRAFT_489380
NPA: UCRNP2_2755
TML: GSTUM_00005882001
SPO: SPAC19A8.04(erg5)
CNE: CNF03720
CNB: CNBF1100
CNG: CNAG_06644
CGI: CGB_F1500C
TMS: TREMEDRAFT_40696
TASA: A1Q1_00690
TVS: TRAVEDRAFT_140384
DSQ: DICSQDRAFT_66936
PCO: PHACADRAFT_246788
SHS: STEHIDRAFT_123767
HIR: HETIRDRAFT_141009(cpm111)
PSQ: PUNSTDRAFT_59236
ADL: AURDEDRAFT_109660
FME: FOMMEDRAFT_106824 FOMMEDRAFT_155371
GTR: GLOTRDRAFT_53715
LBC: LACBIDRAFT_187166(ERG5)
CCI: CC1G_01671
ABP: AGABI1DRAFT66440(AGABI1DRAFT_66440)
ABV: AGABI2DRAFT189837(AGABI2DRAFT_189837)
PCUB: JR316_0000711
MRR: Moror_12729
MORE: E1B28_009893
SCM: SCHCO_02609502(SCHCODRAFT_02609502)
CPUT: CONPUDRAFT_94730
SLA: SERLADRAFT_459032
WSE: WALSEDRAFT_69870
WIC: J056_001310
UMA: UMAG_00350
PFP: PFL1_03644
MGL: MGL_0310
MRT: MRET_0994
MSYM: MSY001_2710
 » show all
Reference
1  [PMID:8543054]
  Authors
Kelly SL, Lamb DC, Corran AJ, Baldwin BC, Parks LW, Kelly DE
  Title
Purification and reconstitution of activity of Saccharomyces cerevisiae P450 61,  a sterol delta 22-desaturase.
  Journal
FEBS Lett 377:217-20 (1995)
DOI:10.1016/0014-5793(95)01342-3
Reference
2  [PMID:8635732]
  Authors
Skaggs BA, Alexander JF, Pierson CA, Schweitzer KS, Chun KT, Koegel C, Barbuch R, Bard M
  Title
Cloning and characterization of the Saccharomyces cerevisiae C-22 sterol desaturase gene, encoding a second cytochrome P-450 involved in ergosterol biosynthesis.
  Journal
Gene 169:105-9 (1996)
DOI:10.1016/0378-1119(95)00770-9
  Sequence
[sce:YMR015C]
Reference
3  [PMID:16531502]
  Authors
Morikawa T, Mizutani M, Aoki N, Watanabe B, Saga H, Saito S, Oikawa A, Suzuki H, Sakurai N, Shibata D, Wadano A, Sakata K, Ohta D.
  Title
Cytochrome P450 CYP710A encodes the sterol C-22 desaturase in Arabidopsis and tomato.
  Journal
Plant Cell 18:1008-22 (2006)
DOI:10.1105/tpc.105.036012
  Sequence
[ath:AT2G34500 AT2G34490]
Other DBs
ExplorEnz - The Enzyme Database: 1.14.19.41
IUBMB Enzyme Nomenclature: 1.14.19.41
ExPASy - ENZYME nomenclature database: 1.14.19.41
BRENDA, the Enzyme Database: 1.14.19.41
LinkDB

KEGG   REACTION: R07489
Entry
R07489                      Reaction                               
Name
beta-sitosterol,NADPH:oxygen oxidoreductase (22,23-dehydrogenating)
Definition
beta-Sitosterol + NADPH + H+ + Oxygen <=> Stigmasterol + NADP+ + 2 H2O
Equation
C01753 + C00005 + C00080 + C00007 <=> C05442 + C00006 + 2 C00001
Reaction class
RC00001  C00005_C00006
RC01886  C01753_C05442
Enzyme
1.14.19.41
Pathway
rn00100  Steroid biosynthesis
rn01100  Metabolic pathways
rn01110  Biosynthesis of secondary metabolites
Orthology
K09832  sterol 22-desaturase [EC:1.14.19.41]
Other DBs
RHEA: 46579
LinkDB

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