KEGG   ORTHOLOGY: K10438
Entry
K10438                      KO                                     
Symbol
PHACB, CYP504B1
Name
3-hydroxyphenylacetate 6-hydroxylase [EC:1.14.13.63]
Pathway
map00643  Styrene degradation
map01120  Microbial metabolism in diverse environments
Reaction
R02517  3-hydroxyphenylacetate,NADPH:oxygen oxidoreductase (6-hydroxylating)
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09111 Xenobiotics biodegradation and metabolism
   00643 Styrene degradation
    K10438  PHACB, CYP504B1; 3-hydroxyphenylacetate 6-hydroxylase
 09180 Brite Hierarchies
  09181 Protein families: metabolism
   00199 Cytochrome P450
    K10438  PHACB, CYP504B1; 3-hydroxyphenylacetate 6-hydroxylase
Enzymes [BR:ko01000]
 1. Oxidoreductases
  1.14  Acting on paired donors, with incorporation or reduction of molecular oxygen
   1.14.13  With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
    1.14.13.63  3-hydroxyphenylacetate 6-hydroxylase
     K10438  PHACB, CYP504B1; 3-hydroxyphenylacetate 6-hydroxylase
Cytochrome P450 [BR:ko00199]
 Cytochrome P450, fungi type
  CYP504 family
   K10438  PHACB, CYP504B1; 3-hydroxyphenylacetate 6-hydroxylase
Other DBs
COG: COG2124
GO: 0047094
Genes
SLB: AWJ20_2761 AWJ20_5196(ERG5)
MGR: MGG_03352
PPEI: PpBr36_06495
PGRI: PgNI_00700
FGR: FGSG_06447
FPU: FPSE_10099
FVN: FVRRES_11114
FVR: FVEG_04417
FOX: FOXG_07489 FOXG_10783
NHE: NECHADRAFT_53600 NECHADRAFT_87111
FFC: NCS54_01382600 NCS54_01416300
FKR: NCS57_01363400 NCS57_01398000
FMU: J7337_006456
MAW: 90961934(J3458_002773)
MAJ: MAA_00010 MAA_04437 MAA_06936
MBRN: 26245670(phacB_3) 26246417(phacB_0)
PCHM: VFPPC_09674
CMT: CCM_02612 CCM_07933
AMUS: LMH87_002934 LMH87_004841 LMH87_008787
PLJ: 28886906(PLICBS_002225)
PTKZ: JDV02_000876 JDV02_002798
VAL: VDBG_09163
VDA: VDAG_07313
CFJ: CFIO01_02908 CFIO01_07804 CFIO01_13056
CLUP: CLUP02_09160 CLUP02_18154
CHIG: CH63R_07166 CH63R_10851 CH63R_13633 CH63R_13746
CDET: 87943931(CDEST_07428) 87944340(CDEST_07837) 87947212(CDEST_10712) 87947327(CDEST_10827)
SAPO: SAPIO_CDS1218
ELA: UCREL1_2048 UCREL1_4076 UCREL1_7322
PFY: PFICI_01247 PFICI_01707
PSCO: LY89DRAFT_302569 LY89DRAFT_594882
ANI: ANIA_01397
AFM: AFUA_1G08800
ACT: ACLA_026760
NFI: NFIA_016920
AOR: AO090005001647
ANG: An08g00180
AFV: AFLA_004481
ALUC: AKAW2_30954S
ACHE: ACHE_31006S
APUU: APUU_10469A APUU_50302A APUU_80788S
PCS: N7525_011503
PDP: PDIP_41800
POU: POX_f08321
TRG: TRUGW13939_07309 TRUGW13939_08977
CIM: CIMG_13120(CIMG07204)
CPW: 9695834(D8B26_006870)
URE: UREG_05702
ABE: ARB_02605
PBL: PAAG_11576(PAAG_02928)
PNO: SNOG_13913
PTE: PTT_06974
PTRR: 6347358(PtrM4_035530)
BZE: COCCADRAFT_111657
BSC: COCSADRAFT_214700
BOR: COCMIDRAFT_41589
AALT: CC77DRAFT_1022627
ARAB: EKO05_0001002 EKO05_0009352
PFJ: MYCFIDRAFT_83850
CBET: CB0940_05131 CB0940_07072
NPA: UCRNP2_1523
 » show all
Reference
PMID:17189487
  Authors
Ferrer-Sevillano F, Fernandez-Canon JM.
  Title
Novel phacB-encoded cytochrome P450 monooxygenase from Aspergillus nidulans with 3-hydroxyphenylacetate 6-hydroxylase and 3,4-dihydroxyphenylacetate 6-hydroxylase activities.
  Journal
Eukaryot Cell 6:514-20 (2007)
DOI:10.1128/EC.00226-06
  Sequence
[ani:ANIA_01397]
LinkDB

KEGG   ENZYME: 1.14.13.63
Entry
EC 1.14.13.63               Enzyme                                 
Name
3-hydroxyphenylacetate 6-hydroxylase;
3-hydroxyphenylacetate 6-monooxygenase
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
Sysname
3-hydroxyphenylacetate,NAD(P)H:oxygen oxidoreductase (6-hydroxylating)
Reaction(IUBMB)
3-hydroxyphenylacetate + NAD(P)H + H+ + O2 = 2,5-dihydroxyphenylacetate + NAD(P)+ + H2O [RN:R02515 R02517]
Reaction(KEGG)
R02515 R02517
Substrate
3-hydroxyphenylacetate [CPD:C05593];
NADH [CPD:C00004];
NADPH [CPD:C00005];
H+ [CPD:C00080];
O2 [CPD:C00007]
Product
2,5-dihydroxyphenylacetate [CPD:C00544];
NAD+ [CPD:C00003];
NADP+ [CPD:C00006];
H2O [CPD:C00001]
Comment
3-hydroxyphenylacetate 6-hydroxylase from Flavobacterium sp. is highly specific for 3-hydroxyphenylacetate and uses NADH and NADPH as electron donors with similar efficiency.
History
EC 1.14.13.63 created 1999
Pathway
ec00643  Styrene degradation
ec01120  Microbial metabolism in diverse environments
Orthology
K10438  3-hydroxyphenylacetate 6-hydroxylase
Genes
SLB: AWJ20_2761 AWJ20_5196(ERG5)
MGR: MGG_03352
PPEI: PpBr36_06495
PGRI: PgNI_00700
FGR: FGSG_06447
FPU: FPSE_10099
FVN: FVRRES_11114
FVR: FVEG_04417
FOX: FOXG_07489 FOXG_10783
NHE: NECHADRAFT_53600 NECHADRAFT_87111
FFC: NCS54_01382600 NCS54_01416300
FKR: NCS57_01363400 NCS57_01398000
FMU: J7337_006456
MAW: 90961934(J3458_002773)
MAJ: MAA_00010 MAA_04437 MAA_06936
MBRN: 26245670(phacB_3) 26246417(phacB_0)
PCHM: VFPPC_09674
CMT: CCM_02612 CCM_07933
AMUS: LMH87_002934 LMH87_004841 LMH87_008787
PLJ: 28886906(PLICBS_002225)
PTKZ: JDV02_000876 JDV02_002798
VAL: VDBG_09163
VDA: VDAG_07313
CFJ: CFIO01_02908 CFIO01_07804 CFIO01_13056
CLUP: CLUP02_09160 CLUP02_18154
CHIG: CH63R_07166 CH63R_10851 CH63R_13633 CH63R_13746
CDET: 87943931(CDEST_07428) 87944340(CDEST_07837) 87947212(CDEST_10712) 87947327(CDEST_10827)
SAPO: SAPIO_CDS1218
ELA: UCREL1_2048 UCREL1_4076 UCREL1_7322
PFY: PFICI_01247 PFICI_01707
PSCO: LY89DRAFT_302569 LY89DRAFT_594882
ANI: ANIA_01397
AFM: AFUA_1G08800
ACT: ACLA_026760
NFI: NFIA_016920
AOR: AO090005001647
ANG: An08g00180
AFV: AFLA_004481
ALUC: AKAW2_30954S
ACHE: ACHE_31006S
APUU: APUU_10469A APUU_50302A APUU_80788S
PCS: N7525_011503
PDP: PDIP_41800
POU: POX_f08321
TRG: TRUGW13939_07309 TRUGW13939_08977
CIM: CIMG_13120(CIMG07204)
CPW: 9695834(D8B26_006870)
URE: UREG_05702
ABE: ARB_02605
PBL: PAAG_11576(PAAG_02928)
PNO: SNOG_13913
PTE: PTT_06974
PTRR: 6347358(PtrM4_035530)
BZE: COCCADRAFT_111657
BSC: COCSADRAFT_214700
BOR: COCMIDRAFT_41589
AALT: CC77DRAFT_1022627
ARAB: EKO05_0001002 EKO05_0009352
PFJ: MYCFIDRAFT_83850
CBET: CB0940_05131 CB0940_07072
NPA: UCRNP2_1523
 » show all
Reference
1  [PMID:1935954]
  Authors
Van Berkel WJ, Van Den Tweel WJ.
  Title
Purification and characterisation of 3-hydroxyphenylacetate 6-hydroxylase: a novel FAD-dependent monooxygenase from a Flavobacterium species.
  Journal
Eur J Biochem 201:585-92 (1991)
DOI:10.1111/j.1432-1033.1991.tb16318.x
Other DBs
ExplorEnz - The Enzyme Database: 1.14.13.63
IUBMB Enzyme Nomenclature: 1.14.13.63
ExPASy - ENZYME nomenclature database: 1.14.13.63
BRENDA, the Enzyme Database: 1.14.13.63
CAS: 114705-01-0
LinkDB

KEGG   REACTION: R02517
Entry
R02517                      Reaction                               
Name
3-hydroxyphenylacetate,NADPH:oxygen oxidoreductase (6-hydroxylating)
Definition
3-Hydroxyphenylacetate + Oxygen + NADPH + H+ <=> Homogentisate + NADP+ + H2O
Equation
C05593 + C00007 + C00005 + C00080 <=> C00544 + C00006 + C00001
Comment
NADH (see R02515)
Reaction class
RC00001  C00005_C00006
RC00046  C00544_C05593
Enzyme
1.14.13.63
Pathway
rn00643  Styrene degradation
rn01120  Microbial metabolism in diverse environments
Brite
Enzymatic reactions [BR:br08201]
 1. Oxidoreductase reactions
  1.14  Acting on paired donors, with incorporation or reduction of molecular oxygen
   1.14.13  With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
    1.14.13.63
     R02517  3-Hydroxyphenylacetate + Oxygen + NADPH + H+ <=> Homogentisate + NADP+ + H2O
Orthology
K10438  3-hydroxyphenylacetate 6-hydroxylase [EC:1.14.13.63]
Other DBs
RHEA: 22207
LinkDB

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