KEGG   ORTHOLOGY: K13229
Entry
K13229                      KO                                     

Name
BX6
Definition
2,4-dihydroxy-1,4-benzoxazin-3-one-glucoside dioxygenase [EC:1.14.11.59]
Pathway
ko00402  Benzoxazinoid biosynthesis
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09110 Biosynthesis of other secondary metabolites
   00402 Benzoxazinoid biosynthesis
    K13229  BX6; 2,4-dihydroxy-1,4-benzoxazin-3-one-glucoside dioxygenase
Enzymes [BR:ko01000]
 1. Oxidoreductases
  1.14  Acting on paired donors, with incorporation or reduction of molecular oxygen
   1.14.11  With 2-oxoglutarate as one donor, and incorporation of one atom of oxygen into each donor
    1.14.11.59  2,4-dihydroxy-1,4-benzoxazin-3-one-glucoside dioxygenase
     K13229  BX6; 2,4-dihydroxy-1,4-benzoxazin-3-one-glucoside dioxygenase
Other DBs
RN: R08972
Genes
ATS: 109766943
SBI: 8070754
ZMA: 541977(bx6)
SITA: 101778204 101781203
Reference
  Authors
Jonczyk R, Schmidt H, Osterrieder A, Fiesselmann A, Schullehner K, Haslbeck M, Sicker D, Hofmann D, Yalpani N, Simmons C, Frey M, Gierl A
  Title
Elucidation of the final reactions of DIMBOA-glucoside biosynthesis in maize: characterization of Bx6 and Bx7.
  Journal
Plant Physiol 146:1053-63 (2008)
DOI:10.1104/pp.107.111237
  Sequence
[zma:541977]
LinkDB

KEGG   ENZYME: 1.14.11.59
Entry
EC 1.14.11.59               Enzyme                                 

Name
2,4-dihydroxy-1,4-benzoxazin-3-one-glucoside dioxygenase;
BX6 (gene name);
DIBOA-Glc dioxygenase
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With 2-oxoglutarate as one donor, and incorporation of one atom of oxygen into each donor
Sysname
(2R)-4-hydroxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl beta-D-glucopyranoside:oxygen oxidoreductase (7-hydroxylating)
Reaction(IUBMB)
(2R)-4-hydroxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl beta-D-glucopyranoside + 2-oxoglutarate + O2 = (2R)-4,7-dihydroxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl beta-D-glucopyranoside + succinate + CO2 + H2O [RN:R08972]
Reaction(KEGG)
R08972
Substrate
(2R)-4-hydroxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl beta-D-glucopyranoside [CPD:C15772];
2-oxoglutarate [CPD:C00026];
O2 [CPD:C00007]
Product
(2R)-4,7-dihydroxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl beta-D-glucopyranoside [CPD:C18062];
succinate [CPD:C00042];
CO2 [CPD:C00011];
H2O [CPD:C00001]
Comment
The enzyme is involved in the biosynthesis of protective and allelophatic benzoxazinoids in some plants, most commonly from the family of Poaceae (grasses).
History
EC 1.14.11.59 created 2012 as EC 1.14.20.2, transferred 2018 to EC 1.14.11.59
Pathway
ec00402  Benzoxazinoid biosynthesis
Orthology
K13229  2,4-dihydroxy-1,4-benzoxazin-3-one-glucoside dioxygenase
Genes
ATS: 109766943
SBI: 8070754
ZMA: 541977(bx6)
SITA: 101778204 101781203
Reference
1  [PMID:18192444]
  Authors
Jonczyk R, Schmidt H, Osterrieder A, Fiesselmann A, Schullehner K, Haslbeck M, Sicker D, Hofmann D, Yalpani N, Simmons C, Frey M, Gierl A
  Title
Elucidation of the final reactions of DIMBOA-glucoside biosynthesis in maize: characterization of Bx6 and Bx7.
  Journal
Plant Physiol 146:1053-63 (2008)
DOI:10.1104/pp.107.111237
  Sequence
[zma:541977]
Other DBs
ExplorEnz - The Enzyme Database: 1.14.11.59
IUBMB Enzyme Nomenclature: 1.14.11.59
ExPASy - ENZYME nomenclature database: 1.14.11.59
BRENDA, the Enzyme Database: 1.14.11.59
LinkDB

KEGG   REACTION: R08972
Entry
R08972                      Reaction                               

Name
(2R)-4-hydroxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl beta-D-glucopyranoside:oxygen oxidoreductase (7-hydroxylating)
Definition
DIBOA-glucoside + 2-Oxoglutarate + Oxygen <=> TRIBOA-glucoside + Succinate + CO2
Equation
Comment
2-oxoglutarate-dependent dioxygenase BX6
Reaction class
RC00490  C15772_C18062
Enzyme
Pathway
rn00402  Benzoxazinoid biosynthesis
Orthology
K13229  2,4-dihydroxy-1,4-benzoxazin-3-one-glucoside dioxygenase [EC:1.14.11.59]
Other DBs
RHEA: 32118
LinkDB

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