KEGG   ORTHOLOGY: K13229
Entry
K13229                      KO                                     
Symbol
BX6
Name
2,4-dihydroxy-1,4-benzoxazin-3-one-glucoside dioxygenase [EC:1.14.11.59]
Pathway
map00402  Benzoxazinoid biosynthesis
map00999  Biosynthesis of various plant secondary metabolites
map01100  Metabolic pathways
map01110  Biosynthesis of secondary metabolites
Module
M00952  Benzoxazinoid biosynthesis, indoleglycerol phosphate => DIMBOA-glucoside
Reaction
R08972  (2R)-4-hydroxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl beta-D-glucopyranoside:oxygen oxidoreductase (7-hydroxylating)
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09110 Biosynthesis of other secondary metabolites
   00402 Benzoxazinoid biosynthesis
    K13229  BX6; 2,4-dihydroxy-1,4-benzoxazin-3-one-glucoside dioxygenase
   00999 Biosynthesis of various plant secondary metabolites
    K13229  BX6; 2,4-dihydroxy-1,4-benzoxazin-3-one-glucoside dioxygenase
Enzymes [BR:ko01000]
 1. Oxidoreductases
  1.14  Acting on paired donors, with incorporation or reduction of molecular oxygen
   1.14.11  With 2-oxoglutarate as one donor, and incorporation of one atom of oxygen into each donor
    1.14.11.59  2,4-dihydroxy-1,4-benzoxazin-3-one-glucoside dioxygenase
     K13229  BX6; 2,4-dihydroxy-1,4-benzoxazin-3-one-glucoside dioxygenase
Genes
ATS: 109766943
TDC: 119345335 119361973
SBI: 8070754
ZMA: 541977
SITA: 101778204 101781203
PVIR: 120669653
Reference
PMID:18192444
  Authors
Jonczyk R, Schmidt H, Osterrieder A, Fiesselmann A, Schullehner K, Haslbeck M, Sicker D, Hofmann D, Yalpani N, Simmons C, Frey M, Gierl A
  Title
Elucidation of the final reactions of DIMBOA-glucoside biosynthesis in maize: characterization of Bx6 and Bx7.
  Journal
Plant Physiol 146:1053-63 (2008)
DOI:10.1104/pp.107.111237
  Sequence
[zma:541977]
LinkDB

KEGG   ENZYME: 1.14.11.59
Entry
EC 1.14.11.59               Enzyme                                 
Name
2,4-dihydroxy-1,4-benzoxazin-3-one-glucoside dioxygenase;
BX6 (gene name);
DIBOA-Glc dioxygenase
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With 2-oxoglutarate as one donor, and incorporation of one atom of oxygen into each donor
Sysname
(2R)-4-hydroxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl beta-D-glucopyranoside:oxygen oxidoreductase (7-hydroxylating)
Reaction(IUBMB)
(2R)-4-hydroxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl beta-D-glucopyranoside + 2-oxoglutarate + O2 = (2R)-4,7-dihydroxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl beta-D-glucopyranoside + succinate + CO2 + H2O [RN:R08972]
Reaction(KEGG)
R08972
Substrate
(2R)-4-hydroxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl beta-D-glucopyranoside [CPD:C15772];
2-oxoglutarate [CPD:C00026];
O2 [CPD:C00007]
Product
(2R)-4,7-dihydroxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl beta-D-glucopyranoside [CPD:C18062];
succinate [CPD:C00042];
CO2 [CPD:C00011];
H2O [CPD:C00001]
Comment
The enzyme is involved in the biosynthesis of protective and allelophatic benzoxazinoids in some plants, most commonly from the family of Poaceae (grasses).
History
EC 1.14.11.59 created 2012 as EC 1.14.20.2, transferred 2018 to EC 1.14.11.59
Pathway
ec00402  Benzoxazinoid biosynthesis
ec00999  Biosynthesis of various plant secondary metabolites
ec01100  Metabolic pathways
ec01110  Biosynthesis of secondary metabolites
Orthology
K13229  2,4-dihydroxy-1,4-benzoxazin-3-one-glucoside dioxygenase
Genes
ATS: 109766943
TDC: 119345335 119361973
SBI: 8070754
ZMA: 541977
SITA: 101778204 101781203
PVIR: 120669653
Reference
1  [PMID:18192444]
  Authors
Jonczyk R, Schmidt H, Osterrieder A, Fiesselmann A, Schullehner K, Haslbeck M, Sicker D, Hofmann D, Yalpani N, Simmons C, Frey M, Gierl A
  Title
Elucidation of the final reactions of DIMBOA-glucoside biosynthesis in maize: characterization of Bx6 and Bx7.
  Journal
Plant Physiol 146:1053-63 (2008)
DOI:10.1104/pp.107.111237
  Sequence
[zma:541977]
Other DBs
ExplorEnz - The Enzyme Database: 1.14.11.59
IUBMB Enzyme Nomenclature: 1.14.11.59
ExPASy - ENZYME nomenclature database: 1.14.11.59
BRENDA, the Enzyme Database: 1.14.11.59
LinkDB

KEGG   REACTION: R08972
Entry
R08972                      Reaction                               
Name
(2R)-4-hydroxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl beta-D-glucopyranoside:oxygen oxidoreductase (7-hydroxylating)
Definition
DIBOA-glucoside + 2-Oxoglutarate + Oxygen <=> TRIBOA-glucoside + Succinate + CO2
Equation
C15772 + C00026 + C00007 <=> C18062 + C00042 + C00011
Comment
2-oxoglutarate-dependent dioxygenase BX6
Reaction class
RC00490  C15772_C18062
Enzyme
1.14.11.59
Pathway
rn00402  Benzoxazinoid biosynthesis
rn00999  Biosynthesis of various plant secondary metabolites
rn01100  Metabolic pathways
rn01110  Biosynthesis of secondary metabolites
Module
M00952  Benzoxazinoid biosynthesis, indoleglycerol phosphate => DIMBOA-glucoside
Brite
Enzymatic reactions [BR:br08201]
 1. Oxidoreductase reactions
  1.14  Acting on paired donors, with incorporation or reduction of molecular oxygen
   1.14.11  With 2-oxoglutarate as one donor, and incorporation of one atom of oxygen into each donor
    1.14.11.59
     R08972  DIBOA-glucoside + 2-Oxoglutarate + Oxygen <=> TRIBOA-glucoside + Succinate + CO2
Orthology
K13229  2,4-dihydroxy-1,4-benzoxazin-3-one-glucoside dioxygenase [EC:1.14.11.59]
Other DBs
RHEA: 32118
LinkDB

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