KEGG   ORTHOLOGY: K15766
Entry
K15766            Tight     KO                                     
Symbol
absAa
Name
2-aminobenzenesulfonate 2,3-dioxygenase subunit alpha [EC:1.14.12.14]
Pathway
map00362  Benzoate degradation
map00623  Toluene degradation
map01100  Metabolic pathways
map01120  Microbial metabolism in diverse environments
Reaction
R05156  2-aminobenzenesulfonate,NADH:oxygen oxidoreductase (2,3-hydroxylating, ammonia-forming)
R05294  toluene-4-sulfonate,NADH:oxygen oxidoreductase (3,4-hydroxylating, sulfite-forming)
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09111 Xenobiotics biodegradation and metabolism
   00362 Benzoate degradation
    K15766  absAa; 2-aminobenzenesulfonate 2,3-dioxygenase subunit alpha
   00623 Toluene degradation
    K15766  absAa; 2-aminobenzenesulfonate 2,3-dioxygenase subunit alpha
Enzymes [BR:ko01000]
 1. Oxidoreductases
  1.14  Acting on paired donors, with incorporation or reduction of molecular oxygen
   1.14.12  With NADH or NADPH as one donor, and incorporation of two atoms of oxygen into the other donor
    1.14.12.14  2-aminobenzenesulfonate 2,3-dioxygenase
     K15766  absAa; 2-aminobenzenesulfonate 2,3-dioxygenase subunit alpha
Other DBs
GO: 0018627
Genes
AG: AAF14227(absAa)
Reference
PMID:10589735
  Authors
Mampel J, Ruff J, Junker F, Cook AM.
  Title
The oxygenase component of the 2-aminobenzenesulfonate dioxygenase system from Alcaligenes sp. strain O-1.
  Journal
Microbiology 145 ( Pt 11):3255-64 (1999)
DOI:10.1099/00221287-145-11-3255
  Sequence
[ag:AAF14227]
Reference
PMID:8075807
  Authors
Junker F, Leisinger T, Cook AM
  Title
3-Sulphocatechol 2,3-dioxygenase and other dioxygenases (EC 1.13.11.2 and EC 1.14.12.-) in the degradative pathways of 2-aminobenzenesulphonic, benzenesulphonic and 4-toluenesulphonic acids in Alcaligenes sp. strain O-1.
  Journal
Microbiology 140 ( Pt 7):1713-22 (1994)
DOI:10.1099/13500872-140-7-1713
  Sequence
[ag:AAF14227]
LinkDB

KEGG   ORTHOLOGY: K15767
Entry
K15767            Tight     KO                                     
Symbol
absAb
Name
2-aminobenzenesulfonate 2,3-dioxygenase subunit beta [EC:1.14.12.14]
Pathway
map00362  Benzoate degradation
map00623  Toluene degradation
map01100  Metabolic pathways
map01120  Microbial metabolism in diverse environments
Reaction
R05156  2-aminobenzenesulfonate,NADH:oxygen oxidoreductase (2,3-hydroxylating, ammonia-forming)
R05294  toluene-4-sulfonate,NADH:oxygen oxidoreductase (3,4-hydroxylating, sulfite-forming)
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09111 Xenobiotics biodegradation and metabolism
   00362 Benzoate degradation
    K15767  absAb; 2-aminobenzenesulfonate 2,3-dioxygenase subunit beta
   00623 Toluene degradation
    K15767  absAb; 2-aminobenzenesulfonate 2,3-dioxygenase subunit beta
Enzymes [BR:ko01000]
 1. Oxidoreductases
  1.14  Acting on paired donors, with incorporation or reduction of molecular oxygen
   1.14.12  With NADH or NADPH as one donor, and incorporation of two atoms of oxygen into the other donor
    1.14.12.14  2-aminobenzenesulfonate 2,3-dioxygenase
     K15767  absAb; 2-aminobenzenesulfonate 2,3-dioxygenase subunit beta
Other DBs
GO: 0018627
Genes
AG: AAF14228(absAb)
Reference
PMID:10589735
  Authors
Mampel J, Ruff J, Junker F, Cook AM.
  Title
The oxygenase component of the 2-aminobenzenesulfonate dioxygenase system from Alcaligenes sp. strain O-1.
  Journal
Microbiology 145 ( Pt 11):3255-64 (1999)
DOI:10.1099/00221287-145-11-3255
  Sequence
[ag:AAF14228]
Reference
PMID:8075807
  Authors
Junker F, Leisinger T, Cook AM
  Title
3-Sulphocatechol 2,3-dioxygenase and other dioxygenases (EC 1.13.11.2 and EC 1.14.12.-) in the degradative pathways of 2-aminobenzenesulphonic, benzenesulphonic and 4-toluenesulphonic acids in Alcaligenes sp. strain O-1.
  Journal
Microbiology 140 ( Pt 7):1713-22 (1994)
DOI:10.1099/13500872-140-7-1713
  Sequence
[ag:AAF14228]
LinkDB

KEGG   ENZYME: 1.14.12.14
Entry
EC 1.14.12.14               Enzyme                                 
Name
2-aminobenzenesulfonate 2,3-dioxygenase;
2-aminosulfobenzene 2,3-dioxygenase
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of two atoms of oxygen into the other donor
Sysname
2-aminobenzenesulfonate,NADH:oxygen oxidoreductase (2,3-hydroxylating, ammonia-forming)
Reaction(IUBMB)
2-aminobenzenesulfonate + NADH + H+ + O2 = 2,3-dihydroxybenzenesulfonate + NH3 + NAD+ [RN:R05156]
Reaction(KEGG)
R05156;
(other) R05294
Substrate
2-aminobenzenesulfonate [CPD:C06333];
NADH [CPD:C00004];
H+ [CPD:C00080];
O2 [CPD:C00007]
Product
2,3-dihydroxybenzenesulfonate [CPD:C06336];
NH3 [CPD:C00014];
NAD+ [CPD:C00003]
History
EC 1.14.12.14 created 1999
Pathway
ec00362  Benzoate degradation
ec00623  Toluene degradation
ec01100  Metabolic pathways
ec01120  Microbial metabolism in diverse environments
Orthology
K15766  2-aminobenzenesulfonate 2,3-dioxygenase subunit alpha
K15767  2-aminobenzenesulfonate 2,3-dioxygenase subunit beta
Reference
1  [PMID:8002948]
  Authors
Junker F, Field JA, Bangerter F, Ramsteiner K, Kohler HP, Joannou CL, Mason JR, Leisinger T, Cook AM.
  Title
Oxygenation and spontaneous deamination of 2-aminobenzenesulphonic acid in Alcaligenes sp. strain O-1 with subsequent meta ring cleavage and spontaneous desulphonation to 2-hydroxymuconic acid.
  Journal
Biochem J 300 ( Pt 2):429-36 (1994)
DOI:10.1042/bj3000429
Reference
2  [PMID:8075807]
  Authors
Junker F, Leisinger T, Cook AM
  Title
3-Sulphocatechol 2,3-dioxygenase and other dioxygenases (EC 1.13.11.2 and EC 1.14.12.-) in the degradative pathways of 2-aminobenzenesulphonic, benzenesulphonic and 4-toluenesulphonic acids in Alcaligenes sp. strain O-1.
  Journal
Microbiology 140 ( Pt 7):1713-22 (1994)
DOI:10.1099/13500872-140-7-1713
  Sequence
[ag:AAF14227 AAF14228]
Other DBs
ExplorEnz - The Enzyme Database: 1.14.12.14
IUBMB Enzyme Nomenclature: 1.14.12.14
ExPASy - ENZYME nomenclature database: 1.14.12.14
UM-BBD (Biocatalysis/Biodegradation Database): 1.14.12.14
BRENDA, the Enzyme Database: 1.14.12.14
CAS: 156621-16-8
LinkDB

KEGG   REACTION: R05294
Entry
R05294                      Reaction                               
Name
toluene-4-sulfonate,NADH:oxygen oxidoreductase (3,4-hydroxylating, sulfite-forming)
Definition
Toluene-4-sulfonate + Oxygen + NADH + H+ <=> 4-Methylcatechol + Sulfite + NAD+
Equation
C06677 + C00007 + C00004 + C00080 <=> C06730 + C00094 + C00003
Reaction class
RC00001  C00003_C00004
RC00570  C06677_C06730
Enzyme
1.14.12.14
Pathway
rn00623  Toluene degradation
rn01100  Metabolic pathways
rn01120  Microbial metabolism in diverse environments
Brite
Enzymatic reactions [BR:br08201]
 1. Oxidoreductase reactions
  1.14  Acting on paired donors, with incorporation or reduction of molecular oxygen
   1.14.12  With NADH or NADPH as one donor, and incorporation of two atoms of oxygen into the other donor
    1.14.12.14
     R05294  Toluene-4-sulfonate + Oxygen + NADH + H+ <=> 4-Methylcatechol + Sulfite + NAD+
Orthology
K15766  2-aminobenzenesulfonate 2,3-dioxygenase subunit alpha [EC:1.14.12.14]
K15767  2-aminobenzenesulfonate 2,3-dioxygenase subunit beta [EC:1.14.12.14]
LinkDB

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