KEGG   ORTHOLOGY: K15944
Entry
K15944                      KO                                     
Symbol
snoaF, dnrE, dauE, aknU
Name
nogalaviketone/aklaviketone reductase [EC:1.1.1.- 1.1.1.362]
Pathway
map01057  Biosynthesis of type II polyketide products
map01100  Metabolic pathways
map01110  Biosynthesis of secondary metabolites
Module
M00781  Nogalavinone/aklavinone biosynthesis, deoxynogalonate/deoxyaklanonate => nogalavinone/aklavinone
Reaction
R06677  auramycinone:NADP+ 7-oxidoreductase
R06682  aklavinone:NADP+ 7-oxidoreductase
R09329  nogalavinone:NADP+ 7-oxidoreductase
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09109 Metabolism of terpenoids and polyketides
   01057 Biosynthesis of type II polyketide products
    K15944  snoaF, dnrE, dauE, aknU; nogalaviketone/aklaviketone reductase
 09180 Brite Hierarchies
  09181 Protein families: metabolism
   01008 Polyketide biosynthesis proteins
    K15944  snoaF, dnrE, dauE, aknU; nogalaviketone/aklaviketone reductase
Enzymes [BR:ko01000]
 1. Oxidoreductases
  1.1  Acting on the CH-OH group of donors
   1.1.1  With NAD+ or NADP+ as acceptor
    1.1.1.362  aklaviketone reductase
     K15944  snoaF, dnrE, dauE, aknU; nogalaviketone/aklaviketone reductase
Polyketide biosynthesis proteins [BR:ko01008]
 Polyketide tailoring proteins
  Others
   K15944  snoaF, dnrE, dauE, aknU; nogalaviketone/aklaviketone reductase
Genes
SPRI: SPRI_0205 SPRI_7148
SPHW: NFX46_24955
SKA: CP970_39835
SGAL: CP966_29005
STSU: B7R87_22060
SCOE: CP976_32935
SXN: IAG42_36850
SDD: D9753_08660
SNIG: HEK616_47440
SJN: RI060_18340
SHAU: K9S39_40700
SKG: KJK29_16140
TALX: FOF52_07460
ACTQ: OG417_36645
ACYN: ORV05_34120
MAU: Micau_4000
MIL: ML5_4421
MFEU: H1D33_25720
DMAT: Dmats_05325
AG: AAA65202(dnrE) AAB08021(dauE) AAF01806(snoaF) AAF73457(aknU)
 » show all
Reference
PMID:11683270
  Authors
Torkkell S, Kunnari T, Palmu K, Mantsala P, Hakala J, Ylihonko K
  Title
The entire nogalamycin biosynthetic gene cluster of Streptomyces nogalater: characterization of a 20-kb DNA region and generation of hybrid structures.
  Journal
Mol Genet Genomics 266:276-88 (2001)
DOI:10.1007/s004380100554
  Sequence
[ag:AAF01806]
Reference
PMID:8655529
  Authors
Dickens ML, Ye J, Strohl WR
  Title
Cloning, sequencing, and analysis of aklaviketone reductase from Streptomyces sp. strain C5.
  Journal
J Bacteriol 178:3384-8 (1996)
DOI:10.1128/JB.178.11.3384-3388.1996
  Sequence
[ag:AAB08021]
Reference
PMID:11016846
  Authors
Raty K, Kunnari T, Hakala J, Mantsala P, Ylihonko K
  Title
A gene cluster from Streptomyces galilaeus involved in glycosylation of aclarubicin.
  Journal
Mol Gen Genet 264:164-72 (2000)
DOI:10.1007/s004380000306
  Sequence
[ag:AAF73457]
LinkDB

KEGG   ENZYME: 1.1.1.362
Entry
EC 1.1.1.362                Enzyme                                 
Name
aklaviketone reductase;
dauE (gene name);
aknU (gene name)
Class
Oxidoreductases;
Acting on the CH-OH group of donors;
With NAD+ or NADP+ as acceptor
Sysname
aklavinone:NADP+ oxidoreductase
Reaction(IUBMB)
aklavinone + NADP+ = aklaviketone + NADPH + H+ [RN:R06682]
Reaction(KEGG)
R06682;
(other) R06677
Substrate
aklavinone [CPD:C12424];
NADP+ [CPD:C00006]
Product
aklaviketone [CPD:C12423];
NADPH [CPD:C00005];
H+ [CPD:C00080]
Comment
The enzyme is involved in the synthesis of the aklavinone aglycone, a common precursor for several anthracycline antibiotics including aclacinomycins, daunorubicin and doxorubicin. The enzyme from the Gram-negative bacterium Streptomyces sp. C5 produces daunomycin.
History
EC 1.1.1.362 created 2013
Pathway
ec01057  Biosynthesis of type II polyketide products
ec01100  Metabolic pathways
ec01110  Biosynthesis of secondary metabolites
Orthology
K15944  nogalaviketone/aklaviketone reductase
Genes
SPRI: SPRI_0205 SPRI_7148
SPHW: NFX46_24955
SKA: CP970_39835
SGAL: CP966_29005
STSU: B7R87_22060
SCOE: CP976_32935
SXN: IAG42_36850
SDD: D9753_08660
SNIG: HEK616_47440
SJN: RI060_18340
SHAU: K9S39_40700
SKG: KJK29_16140
TALX: FOF52_07460
ACTQ: OG417_36645
ACYN: ORV05_34120
MAU: Micau_4000
MIL: ML5_4421
MFEU: H1D33_25720
DMAT: Dmats_05325
 » show all
Reference
1  [PMID:8655529]
  Authors
Dickens ML, Ye J, Strohl WR
  Title
Cloning, sequencing, and analysis of aklaviketone reductase from Streptomyces sp. strain C5.
  Journal
J Bacteriol 178:3384-8 (1996)
DOI:10.1128/JB.178.11.3384-3388.1996
  Sequence
[ag:AAB08021]
Other DBs
ExplorEnz - The Enzyme Database: 1.1.1.362
IUBMB Enzyme Nomenclature: 1.1.1.362
ExPASy - ENZYME nomenclature database: 1.1.1.362
BRENDA, the Enzyme Database: 1.1.1.362
LinkDB

KEGG   REACTION: R06682
Entry
R06682                      Reaction                               
Name
aklavinone:NADP+ 7-oxidoreductase
Definition
Aklaviketone + NADPH + H+ <=> Aklavinone + NADP+
Equation
C12423 + C00005 + C00080 <=> C12424 + C00006
Reaction class
RC00001  C00005_C00006
RC00234  C12423_C12424
Enzyme
1.1.1.362
Pathway
rn01057  Biosynthesis of type II polyketide products
rn01100  Metabolic pathways
rn01110  Biosynthesis of secondary metabolites
Module
M00781  Nogalavinone/aklavinone biosynthesis, deoxynogalonate/deoxyaklanonate => nogalavinone/aklavinone
Brite
Enzymatic reactions [BR:br08201]
 1. Oxidoreductase reactions
  1.1  Acting on the CH-OH group of donors
   1.1.1  With NAD+ or NADP+ as acceptor
    1.1.1.362
     R06682  Aklaviketone + NADPH + H+ <=> Aklavinone + NADP+
Orthology
K15944  nogalaviketone/aklaviketone reductase [EC:1.1.1.- 1.1.1.362]
Other DBs
RHEA: 37414
LinkDB

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