KEGG ORTHOLOGY: K19669

ORTHOLOGY: K19669

Entry

K19669                      KO

Symbol

pphA

Name

phosphonopyruvate hydrolase [EC:3.11.1.3]

Pathway

map00440

Phosphonate and phosphinate metabolism

map01100

Metabolic pathways

Reaction

R08200

Brite

KEGG Orthology (KO) [BR:ko00001]
09100 Metabolism
  09106 Metabolism of other amino acids
   00440 Phosphonate and phosphinate metabolism
    K19669  pphA; phosphonopyruvate hydrolase
Enzymes [BR:ko01000]
3. Hydrolases
  3.11  Acting on carbon-phosphorus bonds
   3.11.1  Acting on carbon-phosphorus bonds (only sub-subclass identified to date)
    3.11.1.3  phosphonopyruvate hydrolase
     K19669  pphA; phosphonopyruvate hydrolase

Other DBs

GO:

0033978

Genes

SLIG:

GTU79_10345

HALF:

QEN58_13060

HAMS:

NF683_11160

BGV:	CAL12_07180

VPD:	VAPA_2c11770(palA)

ETB:	N7L95_23000

MOH:	IHQ72_34655

CHEA:

PVE73_13740

BAUT:

QA635_32865

BXN:	I3J27_33380

BRAZ:

LRP30_31510

RAP:	RHOA_5712(pphA)

AG:	AAO24736(pphA)

» show all

Reference

PMID:12697754

Authors

Kulakova AN, Wisdom GB, Kulakov LA, Quinn JP

Title

The purification and characterization of phosphonopyruvate hydrolase, a novel carbon-phosphorus bond cleavage enzyme from Variovorax sp Pal2.

Journal

J Biol Chem 278:23426-31 (2003)
DOI:10.1074/jbc.M301871200

Sequence

[ag:AAO24736]

LinkDB

ENZYME: 3.11.1.3

Entry

EC 3.11.1.3                 Enzyme

Name

phosphonopyruvate hydrolase;
PPH

Class

Hydrolases;
Acting on carbon-phosphorus bonds;
Acting on carbon-phosphorus bonds (only sub-subclass identified to date)

Reaction(IUBMB)

3-phosphonopyruvate + H2O = pyruvate + phosphate [RN:R08200]

Reaction(KEGG)

R08200

Substrate

3-phosphonopyruvate [CPD:C02798];
H2O [CPD:C00001]

Product

pyruvate [CPD:C00022];
phosphate [CPD:C00009]

Comment

Highly specific for phosphonopyruvate as substrate [2]. The reaction is not inhibited by phosphate but is inhibited by the phosphonates phosphonoformic acid, hydroxymethylphosphonic acid and 3-phosphonopropanoic acid [2]. The enzyme is activated by the divalent cations Co2+, Mg2+ and Mn2+. This enzyme is a member of the phosphoenolpyruvate mutase/isocitrate lyase superfamily [3].

History

EC 3.11.1.3 created 2007

Pathway

ec00440

Phosphonate and phosphinate metabolism

ec01100

Metabolic pathways

Orthology

K19669

phosphonopyruvate hydrolase

Genes

SLIG:

GTU79_10345

HALF:

QEN58_13060

HAMS:

NF683_11160

BGV:	CAL12_07180

VPD:	VAPA_2c11770(palA)

ETB:	N7L95_23000

MOH:	IHQ72_34655

CHEA:

PVE73_13740

BAUT:

QA635_32865

BXN:	I3J27_33380

BRAZ:

LRP30_31510

RAP:	RHOA_5712(pphA)

» show all

Reference

1 [PMID:10648102]

Authors

Ternan NG, Hamilton JT, Quinn JP.

Title

Initial in vitro characterisation of phosphonopyruvate hydrolase, a novel phosphate starvation-independent, carbon-phosphorus bond cleavage enzyme in Burkholderia cepacia Pal6.

Journal

Arch Microbiol 173:35-41 (2000)
DOI:10.1007/s002030050005

Reference

2 [PMID:12697754]

Authors

Kulakova AN, Wisdom GB, Kulakov LA, Quinn JP

Title

The purification and characterization of phosphonopyruvate hydrolase, a novel carbon-phosphorus bond cleavage enzyme from Variovorax sp Pal2.

Journal

J Biol Chem 278:23426-31 (2003)
DOI:10.1074/jbc.M301871200

Sequence

[ag:AAO24736]

Reference

3 [PMID:16981709]

Authors

Chen CC, Han Y, Niu W, Kulakova AN, Howard A, Quinn JP, Dunaway-Mariano D, Herzberg O

Title

Structure and kinetics of phosphonopyruvate hydrolase from Variovorax sp. Pal2: new insight into the divergence of catalysis within the PEP mutase/isocitrate lyase superfamily.

Journal

Biochemistry 45:11491-504 (2006)
DOI:10.1021/bi061208l

Sequence

[ag:AAO24736]

Other DBs

ExplorEnz - The Enzyme Database:

3.11.1.3

IUBMB Enzyme Nomenclature:

3.11.1.3

ExPASy - ENZYME nomenclature database:

3.11.1.3

BRENDA, the Enzyme Database:

3.11.1.3

LinkDB

REACTION: R08200

Entry

R08200                      Reaction

Definition

3-Phosphonopyruvate + H2O <=> Pyruvate + Orthophosphate

Equation

C02798 + C00001 <=> C00022 + C00009

Reaction class

RC02147

C00022_C02798

Enzyme

3.11.1.3

Pathway

rn00440

Phosphonate and phosphinate metabolism

rn01100

Metabolic pathways

Brite

Enzymatic reactions [BR:br08201]
3. Hydrolase reactions
  3.11  Acting on carbon-phosphorus bonds
   3.11.1  Acting on carbon-phosphorus bonds (only sub-subclass identified to date)
    3.11.1.3
     R08200  3-Phosphonopyruvate + H2O <=> Pyruvate + Orthophosphate

Orthology

K19669

phosphonopyruvate hydrolase [EC:3.11.1.3]

Other DBs

RHEA:

16676

LinkDB

DBGET integrated database retrieval system