KEGG   ORTHOLOGY: K20090
Entry
K20090                      KO                                     
Symbol
vioC
Name
violacein synthase [EC:1.14.13.224]
Pathway
map00404  Staurosporine biosynthesis
map01100  Metabolic pathways
map01110  Biosynthesis of secondary metabolites
map02024  Quorum sensing
Module
M00808  Violacein biosynthesis, tryptophan => violacein
Reaction
R11132  protodeoxyviolaceinate,NADPH:O2 oxidoreductase
R11135  protoviolaceinate,NADPH:O2 oxidoreductase
R11373  protoviolaceinate,NADH:O2 oxidoreductase
R11374  protodeoxyviolaceinate,NADH:O2 oxidoreductase
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09110 Biosynthesis of other secondary metabolites
   00404 Staurosporine biosynthesis
    K20090  vioC; violacein synthase
 09140 Cellular Processes
  09145 Cellular community - prokaryotes
   02024 Quorum sensing
    K20090  vioC; violacein synthase
Enzymes [BR:ko01000]
 1. Oxidoreductases
  1.14  Acting on paired donors, with incorporation or reduction of molecular oxygen
   1.14.13  With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
    1.14.13.224  violacein synthase
     K20090  vioC; violacein synthase
Other DBs
COG: COG0654
Genes
CVI: CV_3272(vioC)
CVC: BKX93_07105
CHRI: DK842_01230
CHRB: DK843_06510
CHRM: FYK34_10585
IFL: C1H71_10080
JAG: GJA_1082(vioC)
JAZ: YQ44_10560
JAL: BZG29_16955
JLV: G3257_17685(vioC)
JAH: JAB4_031360(vioC)
JRI: P9875_17640
MNR: ACZ75_19210
MASS: CR152_21625
MANC: IV454_26315
MSD: MYSTI_01836
MLAN: JY572_17185
RIV: Riv7116_3968
 » show all
Reference
PMID:17176066
  Authors
Balibar CJ, Walsh CT
  Title
In vitro biosynthesis of violacein from L-tryptophan by the enzymes VioA-E from Chromobacterium violaceum.
  Journal
Biochemistry 45:15444-57 (2006)
DOI:10.1021/bi061998z
  Sequence
[cvi:CV_3272]
LinkDB

KEGG   ENZYME: 1.14.13.224
Entry
EC 1.14.13.224              Enzyme                                 
Name
violacein synthase;
proviolaceinate monooxygenase;
vioC (gene name)
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
Sysname
protoviolaceinate,NAD(P)H:O2 oxidoreductase
Reaction(IUBMB)
(1) protoviolaceinate + NAD(P)H + O2 = violaceinate + NAD(P)+ + H2O [RN:R11135 R11373];
(2) protodeoxyviolaceinate + NAD(P)H + O2 = deoxyviolaceinate + NAD(P)+ + H2O [RN:R11132 R11374]
Reaction(KEGG)
R11132 R11135 R11373 R11374
Substrate
protoviolaceinate [CPD:C21134];
NADH [CPD:C00004];
NADPH [CPD:C00005];
O2 [CPD:C00007];
protodeoxyviolaceinate [CPD:C21131]
Product
violaceinate [CPD:C21135];
NAD+ [CPD:C00003];
NADP+ [CPD:C00006];
H2O [CPD:C00001];
deoxyviolaceinate [CPD:C21132]
Comment
The enzyme, characterized from the bacterium Chromobacterium violaceum, participates in the biosynthesis of the violet pigment violacein. The products, violaceinate and deoxyviolaceinate, undergo non-enzymic autooxidation into violacein and deoxyviolacein, respectively.
History
EC 1.14.13.224 created 2016
Pathway
ec00404  Staurosporine biosynthesis
ec01100  Metabolic pathways
ec01110  Biosynthesis of secondary metabolites
Orthology
K20090  violacein synthase
Genes
CVI: CV_3272(vioC)
CVC: BKX93_07105
CHRI: DK842_01230
CHRB: DK843_06510
CHRM: FYK34_10585
IFL: C1H71_10080
JAG: GJA_1082(vioC)
JAZ: YQ44_10560
JAL: BZG29_16955
JLV: G3257_17685(vioC)
JAH: JAB4_031360(vioC)
JRI: P9875_17640
MNR: ACZ75_19210
MASS: CR152_21625
MANC: IV454_26315
MSD: MYSTI_01836
MLAN: JY572_17185
RIV: Riv7116_3968
 » show all
Reference
1  [PMID:17176066]
  Authors
Balibar CJ, Walsh CT
  Title
In vitro biosynthesis of violacein from L-tryptophan by the enzymes VioA-E from Chromobacterium violaceum.
  Journal
Biochemistry 45:15444-57 (2006)
DOI:10.1021/bi061998z
Reference
2  [PMID:17925955]
  Authors
Shinoda K, Hasegawa T, Sato H, Shinozaki M, Kuramoto H, Takamiya Y, Sato T, Nikaidou N, Watanabe T, Hoshino T.
  Title
Biosynthesis of violacein: a genuine intermediate, protoviolaceinic acid, produced by VioABDE, and insight into VioC function.
  Journal
10.1039/b705358d
  Sequence
[cvi:CV_3272]
Other DBs
ExplorEnz - The Enzyme Database: 1.14.13.224
IUBMB Enzyme Nomenclature: 1.14.13.224
ExPASy - ENZYME nomenclature database: 1.14.13.224
BRENDA, the Enzyme Database: 1.14.13.224
LinkDB

KEGG   REACTION: R11135
Entry
R11135                      Reaction                               
Name
protoviolaceinate,NADPH:O2 oxidoreductase
Definition
Protoviolaceinic acid + Oxygen + NADPH + H+ <=> Violaceinic acid + NADP+ + H2O
Equation
C21134 + C00007 + C00005 + C00080 <=> C21135 + C00006 + C00001
Reaction class
RC03365  C21134_C21135
Enzyme
1.14.13.224
Pathway
rn00404  Staurosporine biosynthesis
rn01100  Metabolic pathways
rn01110  Biosynthesis of secondary metabolites
Module
M00808  Violacein biosynthesis, tryptophan => violacein
Brite
Enzymatic reactions [BR:br08201]
 1. Oxidoreductase reactions
  1.14  Acting on paired donors, with incorporation or reduction of molecular oxygen
   1.14.13  With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
    1.14.13.224
     R11135  Protoviolaceinic acid + Oxygen + NADPH + H+ <=> Violaceinic acid + NADP+ + H2O
Orthology
K20090  violacein synthase [EC:1.14.13.224]
Other DBs
RHEA: 49123
LinkDB

KEGG   REACTION: R11373
Entry
R11373                      Reaction                               
Name
protoviolaceinate,NADH:O2 oxidoreductase
Definition
Protoviolaceinic acid + Oxygen + NADH + H+ <=> Violaceinic acid + NAD+ + H2O
Equation
C21134 + C00007 + C00004 + C00080 <=> C21135 + C00003 + C00001
Reaction class
RC03365  C21134_C21135
Enzyme
1.14.13.224
Pathway
rn00404  Staurosporine biosynthesis
rn01100  Metabolic pathways
rn01110  Biosynthesis of secondary metabolites
Brite
Enzymatic reactions [BR:br08201]
 1. Oxidoreductase reactions
  1.14  Acting on paired donors, with incorporation or reduction of molecular oxygen
   1.14.13  With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
    1.14.13.224
     R11373  Protoviolaceinic acid + Oxygen + NADH + H+ <=> Violaceinic acid + NAD+ + H2O
Orthology
K20090  violacein synthase [EC:1.14.13.224]
Other DBs
RHEA: 49655
LinkDB

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