KEGG   ORTHOLOGY: K20199
Entry
K20199                      KO                                     
Symbol
pchA
Name
4-hydroxybenzaldehyde dehydrogenase (NADP+) [EC:1.2.1.96]
Pathway
map00623  Toluene degradation
map01120  Microbial metabolism in diverse environments
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09111 Xenobiotics biodegradation and metabolism
   00623 Toluene degradation
    K20199  pchA; 4-hydroxybenzaldehyde dehydrogenase (NADP+)
Enzymes [BR:ko01000]
 1. Oxidoreductases
  1.2  Acting on the aldehyde or oxo group of donors
   1.2.1  With NAD+ or NADP+ as acceptor
    1.2.1.96  4-hydroxybenzaldehyde dehydrogenase (NADP+)
     K20199  pchA; 4-hydroxybenzaldehyde dehydrogenase (NADP+)
Other DBs
RN: R01294 R11195
COG: COG1012
Genes
RTH: LRK53_00385
PFUW: KF707C_49540
PCQ: PcP3B5_13480(yfmT_1)
PPUT: L483_22005
PDR: H681_14070
PALK: PSAKL28_20220 PSAKL28_31420
PSOS: POS17_5138(pcuC)
PSET: THL1_648
PSIL: PMA3_18555
PUM: HGP31_12070
OCE: GU3_14325
EBA: ebA3161(pchA)
CCOE: CETAM_10235(yfmT)
AG: AGO01127(pchA)
 » show all
Reference
  Authors
Chen YF, Chao H, Zhou NY
  Title
The catabolism of 2,4-xylenol and p-cresol share the enzymes for the oxidation of para-methyl group in Pseudomonas putida NCIMB 9866.
  Journal
Appl Microbiol Biotechnol 98:1349-56 (2014)
DOI:10.1007/s00253-013-5001-z
  Sequence
LinkDB

KEGG   ENZYME: 1.2.1.96
Entry
EC 1.2.1.96                 Enzyme                                 
Name
4-hydroxybenzaldehyde dehydrogenase (NADP+);
p-hydroxybenzaldehyde dehydrogenase (ambiguous);
pchA (gene name)
Class
Oxidoreductases;
Acting on the aldehyde or oxo group of donors;
With NAD+ or NADP+ as acceptor
Sysname
4-hydroxybenzaldehyde:NADP+ oxidoreductase
Reaction(IUBMB)
4-hydroxybenzaldehyde + NADP+ + H2O = 4-hydroxybenzoate + NADPH + 2 H+ [RN:R01294]
Reaction(KEGG)
R01294;
(other) R11195
Substrate
4-hydroxybenzaldehyde [CPD:C00633];
NADP+ [CPD:C00006];
H2O [CPD:C00001]
Product
4-hydroxybenzoate [CPD:C00156];
NADPH [CPD:C00005];
H+ [CPD:C00080]
Comment
Involved in the aerobic pathway for degradation of toluene, 4-methylphenol, and 2,4-xylenol by several Pseudomonas strains. The enzyme is also active with 4-hydroxy-3-methylbenzaldehyde. cf. EC 1.2.1.64, 4-hydroxybenzaldehyde dehydrogenase (NAD+).
History
EC 1.2.1.96 created 2015
Pathway
ec00623  Toluene degradation
ec01120  Microbial metabolism in diverse environments
Orthology
K20199  4-hydroxybenzaldehyde dehydrogenase (NADP+)
Genes
RTHLRK53_00385
PFUWKF707C_49540
PCQPcP3B5_13480(yfmT_1)
PPUTL483_22005
PDRH681_14070
PALKPSAKL28_20220 PSAKL28_31420
PSOSPOS17_5138(pcuC)
PSETTHL1_648
PSILPMA3_18555
PUMHGP31_12070
PLALFXN65_13830
PASGKSS96_11230
HSXHNO51_19030
CAMHLCW13_08055
OCEGU3_14325
EBAebA3161(pchA)
TMDKUV46_09890
EACEKKI46_02990
AACOK1I37_11200
MSENK3U95_02165
CCOECETAM_10235(yfmT)
AJGKKR91_02090
GPRJQN66_03880
THAONI17_020775
HTSHMJ29_04580
SABUMBM09_12560
 » show all
Reference
1  [PMID:2019564]
  Authors
Whited GM, Gibson DT.
  Title
Separation and partial characterization of the enzymes of the toluene-4-monooxygenase catabolic pathway in Pseudomonas mendocina KR1.
  Journal
J Bacteriol 173:3017-20 (1991)
DOI:10.1128/JB.173.9.3017-3020.1991
Reference
2  [PMID:23736872]
  Authors
Chen YF, Chao H, Zhou NY
  Title
The catabolism of 2,4-xylenol and p-cresol share the enzymes for the oxidation of para-methyl group in Pseudomonas putida NCIMB 9866.
  Journal
Appl Microbiol Biotechnol 98:1349-56 (2014)
DOI:10.1007/s00253-013-5001-z
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 1.2.1.96
IUBMB Enzyme Nomenclature: 1.2.1.96
ExPASy - ENZYME nomenclature database: 1.2.1.96
BRENDA, the Enzyme Database: 1.2.1.96
CAS: 61229-72-9
LinkDB

KEGG   REACTION: R11195
Entry
R11195                      Reaction                               
Name
4-hydroxy-3-methylbenzaldehyde:NADP+ oxidoreductase
Definition
4-Hydroxy-3-methylbenzaldehyde + NADP+ + H2O <=> 4-Hydroxy-3-methylbenzoate + NADPH + H+
Equation
Reaction class
RC00075  C21166_C21167
Enzyme
Pathway
rn00623  Toluene degradation
rn01120  Microbial metabolism in diverse environments
Orthology
K20199  4-hydroxybenzaldehyde dehydrogenase (NADP+) [EC:1.2.1.96]
LinkDB

DBGET integrated database retrieval system