KEGG   ORTHOLOGY: K21104
Entry
K21104            Tight     KO                                     
Symbol
E3.1.1.101
Name
poly(ethylene terephthalate) hydrolase [EC:3.1.1.101]
Pathway
map00624  Polycyclic aromatic hydrocarbon degradation
map01100  Metabolic pathways
map01120  Microbial metabolism in diverse environments
Reaction
R11540  poly(ethylene terephthalate) hydrolase
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09111 Xenobiotics biodegradation and metabolism
   00624 Polycyclic aromatic hydrocarbon degradation
    K21104  E3.1.1.101; poly(ethylene terephthalate) hydrolase
Enzymes [BR:ko01000]
 3. Hydrolases
  3.1  Acting on ester bonds
   3.1.1  Carboxylic-ester hydrolases
    3.1.1.101  poly(ethylene terephthalate) hydrolase
     K21104  E3.1.1.101; poly(ethylene terephthalate) hydrolase
Genes
AG: GAP38373
Reference
PMID:26965627
  Authors
Yoshida S, Hiraga K, Takehana T, Taniguchi I, Yamaji H, Maeda Y, Toyohara K, Miyamoto K, Kimura Y, Oda K
  Title
A bacterium that degrades and assimilates poly(ethylene terephthalate).
  Journal
Science 351:1196-9 (2016)
DOI:10.1126/science.aad6359
  Sequence
[ag:GAP38373]
LinkDB

KEGG   ENZYME: 3.1.1.101
Entry
EC 3.1.1.101                Enzyme                                 
Name
poly(ethylene terephthalate) hydrolase;
PETase;
PET hydrolase
Class
Hydrolases;
Acting on ester bonds;
Carboxylic-ester hydrolases
Sysname
poly(ethylene terephthalate) hydrolase
Reaction(IUBMB)
(ethylene terephthalate)n + H2O = (ethylene terephthalate)n-1 + 4-[(2-hydroxyethoxy)carbonyl]benzoate [RN:R11540]
Reaction(KEGG)
R11540
Substrate
(ethylene terephthalate)n [CPD:C21451];
H2O [CPD:C00001]
Product
(ethylene terephthalate)n-1;
4-[(2-hydroxyethoxy)carbonyl]benzoate [CPD:C21450]
Comment
The enzyme, isolated from the bacterium Ideonella sakaiensis, also produces small amounts of terephthalate (cf. EC 3.1.1.102, mono(ethylene terephthalate) hydrolase). The reaction takes place on PET-film placed in solution.
History
EC 3.1.1.101 created 2016
Pathway
ec00624  Polycyclic aromatic hydrocarbon degradation
ec01100  Metabolic pathways
ec01120  Microbial metabolism in diverse environments
Orthology
K21104  poly(ethylene terephthalate) hydrolase
Reference
1  [PMID:26965627]
  Authors
Yoshida S, Hiraga K, Takehana T, Taniguchi I, Yamaji H, Maeda Y, Toyohara K, Miyamoto K, Kimura Y, Oda K
  Title
A bacterium that degrades and assimilates poly(ethylene terephthalate).
  Journal
Science 351:1196-9 (2016)
DOI:10.1126/science.aad6359
  Sequence
[ag:GAP38373]
Other DBs
ExplorEnz - The Enzyme Database: 3.1.1.101
IUBMB Enzyme Nomenclature: 3.1.1.101
ExPASy - ENZYME nomenclature database: 3.1.1.101
BRENDA, the Enzyme Database: 3.1.1.101
LinkDB

KEGG   REACTION: R11540
Entry
R11540                      Reaction                               
Name
poly(ethylene terephthalate) hydrolase
Definition
Poly(ethylene terephthalate)(n) + H2O <=> Poly(ethylene terephthalate)(n-1) + 4-(2-Hydroxyethoxycarbonyl)benzoate
Equation
C21451(n) + C00001 <=> C21451(n-1) + C21450
Enzyme
3.1.1.101
Pathway
rn00624  Polycyclic aromatic hydrocarbon degradation
rn01100  Metabolic pathways
rn01120  Microbial metabolism in diverse environments
Brite
Enzymatic reactions [BR:br08201]
 3. Hydrolase reactions
  3.1  Acting on ester bonds
   3.1.1  Carboxylic-ester hydrolases
    3.1.1.101
     R11540  Poly(ethylene terephthalate)(n) + H2O <=> Poly(ethylene terephthalate)(n-1) + 4-(2-Hydroxyethoxycarbonyl)benzoate
Orthology
K21104  poly(ethylene terephthalate) hydrolase [EC:3.1.1.101]
Other DBs
RHEA: 49531
LinkDB

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