KEGG   ORTHOLOGY: K21205
Entry
K21205            Tight     KO                                     
Symbol
CYP71CU1
Name
bursehernin 5'-monooxygenase [EC:1.14.14.131]
Pathway
map00999  Biosynthesis of various plant secondary metabolites
map01100  Metabolic pathways
map01110  Biosynthesis of secondary metabolites
Module
M00902  Podophyllotoxin biosynthesis, coniferyl alcohol => podophyllotoxin
Reaction
R11211  (-)-bursehernin,[reduced NADPH---hemoprotein reductase]:oxygen oxidoreductase (5'-hydroxylating)
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09110 Biosynthesis of other secondary metabolites
   00999 Biosynthesis of various plant secondary metabolites
    K21205  CYP71CU1; bursehernin 5'-monooxygenase
 09180 Brite Hierarchies
  09181 Protein families: metabolism
   00199 Cytochrome P450
    K21205  CYP71CU1; bursehernin 5'-monooxygenase
Enzymes [BR:ko01000]
 1. Oxidoreductases
  1.14  Acting on paired donors, with incorporation or reduction of molecular oxygen
   1.14.14  With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
    1.14.14.131  bursehernin 5'-monooxygenase
     K21205  CYP71CU1; bursehernin 5'-monooxygenase
Cytochrome P450 [BR:ko00199]
 Cytochrome P450, plant type
  CYP71 family
   K21205  CYP71CU1; bursehernin 5'-monooxygenase
Genes
AG: ALG05134(CYP71CU1)
Reference
PMID:26359402
  Authors
Lau W, Sattely ES
  Title
Six enzymes from mayapple that complete the biosynthetic pathway to the etoposide aglycone.
  Journal
Science 349:1224-8 (2015)
DOI:10.1126/science.aac7202
  Sequence
[ag:ALG05134]
LinkDB

KEGG   ENZYME: 1.14.14.131
Entry
EC 1.14.14.131              Enzyme                                 
Name
bursehernin 5'-monooxygenase;
CYP71CU1 (gene name);
bursehernin 5'-hydroxylase
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
Sysname
(-)-bursehernin,[reduced NADPH---hemoprotein reductase]:oxygen oxidoreductase (5'-hydroxylating)
Reaction(IUBMB)
(-)-bursehernin + [reduced NADPH---hemoprotein reductase] + O2 = (-)-5'-demethylyatein + [oxidized NADPH---hemoprotein reductase] + H2O [RN:R11211]
Reaction(KEGG)
R11211
Substrate
(-)-bursehernin [CPD:C21183];
[reduced NADPH---hemoprotein reductase] [CPD:C03024];
O2 [CPD:C00007]
Product
(-)-5'-demethylyatein [CPD:C21184];
[oxidized NADPH---hemoprotein reductase] [CPD:C03161];
H2O [CPD:C00001]
Comment
A cytochrome P-450 (heme-thiolate) protein characterized from the plant Sinopodophyllum hexandrum. The enzyme is involved in the biosynthetic pathway of podophyllotoxin, a non-alkaloid toxin lignan whose derivatives are important anticancer drugs.
History
EC 1.14.14.131 created 2016 as EC 1.14.13.213, transferred 2018 to EC 1.14.14.131
Pathway
ec00999  Biosynthesis of various plant secondary metabolites
ec01100  Metabolic pathways
ec01110  Biosynthesis of secondary metabolites
Orthology
K21205  bursehernin 5'-monooxygenase
Reference
1  [PMID:26359402]
  Authors
Lau W, Sattely ES
  Title
Six enzymes from mayapple that complete the biosynthetic pathway to the etoposide aglycone.
  Journal
Science 349:1224-8 (2015)
DOI:10.1126/science.aac7202
  Sequence
[ag:ALG05134]
Other DBs
ExplorEnz - The Enzyme Database: 1.14.14.131
IUBMB Enzyme Nomenclature: 1.14.14.131
ExPASy - ENZYME nomenclature database: 1.14.14.131
BRENDA, the Enzyme Database: 1.14.14.131
LinkDB

KEGG   REACTION: R11211
Entry
R11211                      Reaction                               
Name
(-)-bursehernin,[reduced NADPH---hemoprotein reductase]:oxygen oxidoreductase (5'-hydroxylating)
Definition
Bursehernin + [Reduced NADPH---hemoprotein reductase] + Oxygen <=> (-)-5'-Demethylyatein + [Oxidized NADPH---hemoprotein reductase] + H2O
Equation
C21183 + C03024 + C00007 <=> C21184 + C03161 + C00001
Reaction class
RC00046  C21183_C21184
Enzyme
1.14.14.131
Pathway
rn00999  Biosynthesis of various plant secondary metabolites
rn01100  Metabolic pathways
rn01110  Biosynthesis of secondary metabolites
Module
M00902  Podophyllotoxin biosynthesis, coniferyl alcohol => podophyllotoxin
Brite
Enzymatic reactions [BR:br08201]
 1. Oxidoreductase reactions
  1.14  Acting on paired donors, with incorporation or reduction of molecular oxygen
   1.14.14  With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
    1.14.14.131
     R11211  Bursehernin + [Reduced NADPH---hemoprotein reductase] + Oxygen <=> (-)-5'-Demethylyatein + [Oxidized NADPH---hemoprotein reductase] + H2O
Orthology
K21205  bursehernin 5'-monooxygenase [EC:1.14.14.131]
Other DBs
RHEA: 49031
LinkDB

DBGET integrated database retrieval system