KEGG   ORTHOLOGY: K21207
Entry
K21207            Tight     KO                                     
Symbol
CYP719A23_24
Name
(-)-pluviatolide synthase [EC:1.14.19.72]
Pathway
map00999  Biosynthesis of various plant secondary metabolites
map01100  Metabolic pathways
map01110  Biosynthesis of secondary metabolites
Module
M00902  Podophyllotoxin biosynthesis, coniferyl alcohol => podophyllotoxin
Reaction
R11222  (-)-matairesinol,[reduced NADPH---hemoprotein reductase]:oxygen oxidoreductase (methylenedioxy-bridge-forming)
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09110 Biosynthesis of other secondary metabolites
   00999 Biosynthesis of various plant secondary metabolites
    K21207  CYP719A23_24; (-)-pluviatolide synthase
 09180 Brite Hierarchies
  09181 Protein families: metabolism
   00199 Cytochrome P450
    K21207  CYP719A23_24; (-)-pluviatolide synthase
Enzymes [BR:ko01000]
 1. Oxidoreductases
  1.14  Acting on paired donors, with incorporation or reduction of molecular oxygen
   1.14.19  With oxidation of a pair of donors resulting in the reduction of O2 to two molecules of water
    1.14.19.72  (-)-pluviatolide synthase
     K21207  CYP719A23_24; (-)-pluviatolide synthase
Cytochrome P450 [BR:ko00199]
 Cytochrome P450, plant type
  CYP719 family
   K21207  CYP719A23_24; (-)-pluviatolide synthase
Genes
AG: AGC29953(CYP719A23) AGC29954(CYP719A24)
Reference
PMID:23161544 (CYP719A23 CYP719A24)
  Authors
Marques JV, Kim KW, Lee C, Costa MA, May GD, Crow JA, Davin LB, Lewis NG
  Title
Next generation sequencing in predicting gene function in podophyllotoxin biosynthesis.
  Journal
J Biol Chem 288:466-79 (2013)
DOI:10.1074/jbc.M112.400689
  Sequence
[ag:AGC29953 AGC29954]
LinkDB

KEGG   ENZYME: 1.14.19.72
Entry
EC 1.14.19.72               Enzyme                                 
Name
(-)-pluviatolide synthase;
CYP719A23 (gene name)
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With oxidation of a pair of donors resulting in the reduction of O2 to two molecules of water
Sysname
(-)-matairesinol,[reduced NADPH---hemoprotein reductase]:oxygen oxidoreductase (methylenedioxy-bridge-forming)
Reaction(IUBMB)
(-)-matairesinol + [reduced NADPH---hemoprotein reductase] + O2 = (-)-pluviatolide + [oxidized NADPH---hemoprotein reductase] + 2 H2O [RN:R11222]
Reaction(KEGG)
R11222
Substrate
(-)-matairesinol [CPD:C10682];
[reduced NADPH---hemoprotein reductase] [CPD:C03024];
O2 [CPD:C00007]
Product
(-)-pluviatolide [CPD:C21191];
[oxidized NADPH---hemoprotein reductase] [CPD:C03161];
H2O [CPD:C00001]
Comment
A cytochrome P-450 (heme-thiolate) protein. The enzyme from the plants Sinopodophyllum hexandrum and Podophyllum peltatum catalyses the formation of a methylenedioxy-bridge. It is involved in the biosynthesis of podophyllotoxin, a non-alkaloid toxin lignan whose derivatives are important anticancer drugs.
History
EC 1.14.19.72 created 2016 as EC 1.14.21.11, transferred 2018 to EC 1.14.19.72
Pathway
ec00999  Biosynthesis of various plant secondary metabolites
ec01100  Metabolic pathways
ec01110  Biosynthesis of secondary metabolites
Orthology
K21207  (-)-pluviatolide synthase
Reference
1  [PMID:23161544]
  Authors
Marques JV, Kim KW, Lee C, Costa MA, May GD, Crow JA, Davin LB, Lewis NG
  Title
Next generation sequencing in predicting gene function in podophyllotoxin biosynthesis.
  Journal
J Biol Chem 288:466-79 (2013)
DOI:10.1074/jbc.M112.400689
  Sequence
[ag:AGC29953 AGC29954]
Other DBs
ExplorEnz - The Enzyme Database: 1.14.19.72
IUBMB Enzyme Nomenclature: 1.14.19.72
ExPASy - ENZYME nomenclature database: 1.14.19.72
BRENDA, the Enzyme Database: 1.14.19.72
LinkDB

KEGG   REACTION: R11222
Entry
R11222                      Reaction                               
Name
(-)-matairesinol,[reduced NADPH---hemoprotein reductase]:oxygen oxidoreductase (methylenedioxy-bridge-forming)
Definition
Matairesinol + [Reduced NADPH---hemoprotein reductase] + Oxygen <=> Pluviatolide + [Oxidized NADPH---hemoprotein reductase] + 2 H2O
Equation
C10682 + C03024 + C00007 <=> C21191 + C03161 + 2 C00001
Reaction class
RC00925  C10682_C21191
Enzyme
1.14.19.72
Pathway
rn00999  Biosynthesis of various plant secondary metabolites
rn01100  Metabolic pathways
rn01110  Biosynthesis of secondary metabolites
Module
M00902  Podophyllotoxin biosynthesis, coniferyl alcohol => podophyllotoxin
Brite
Enzymatic reactions [BR:br08201]
 1. Oxidoreductase reactions
  1.14  Acting on paired donors, with incorporation or reduction of molecular oxygen
   1.14.19  With oxidation of a pair of donors resulting in the reduction of O2 to two molecules of water
    1.14.19.72
     R11222  Matairesinol + [Reduced NADPH---hemoprotein reductase] + Oxygen <=> Pluviatolide + [Oxidized NADPH---hemoprotein reductase] + 2 H2O
Orthology
K21207  (-)-pluviatolide synthase [EC:1.14.19.72]
Other DBs
RHEA: 49087
LinkDB

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