Entry
Name
tetrahydroberberine oxidase;
(S)-THB oxidase
Class
Oxidoreductases;
Acting on the CH-CH group of donors;
With oxygen as acceptor
BRITE hierarchy
Sysname
(S)-tetrahydroberberine:oxygen oxidoreductase
Reaction(IUBMB)
(S)-tetrahydroberberine + 2 O2 = berberine + 2 H2O2 [RN:
R02880 ]
Reaction(KEGG)
Substrate
Product
Comment
The enzyme from Berberis sp. is a flavoprotein; that from Coptis japonica is not. (R)-Tetrahydroberberines are not oxidized.
History
EC 1.3.3.8 created 1990 (EC 1.5.3.8 created 1989, incorporated 1992)
Pathway
ec00950 Isoquinoline alkaloid biosynthesis
ec01110 Biosynthesis of secondary metabolites
Orthology
K22089 tetrahydroprotoberberine oxidase
Genes
PSOM : 113274966 113303200
Taxonomy
Reference
1
Authors
Amann M, Nagakura N, Zenk MH.
Title
(S)-Tetrahydroprotoberberine oxidase the final enzyme in protoberberine biosynthesis.
Journal
Tetrahedron Lett 25:953-954 (1984)
Reference
2
Authors
Okada N, Shinmyo A, Okada H, Yamada Y.
Title
Purification and characterization of (S)-tetrahydroberberine oxidase from cultured Coptis japonica cells.
Journal
Phytochemistry 27:979-982 (1988)
Other DBs
ExplorEnz - The Enzyme Database: 1.3.3.8
ExPASy - ENZYME nomenclature database: 1.3.3.8
BRENDA, the Enzyme Database: 1.3.3.8
LinkDB
All DBs