KEGG   REACTION: R12377
Entry
R12377                      Reaction                               
Name
24-methylidenecycloartanol,ferrocytochrome-b5:oxygen oxidoreductase (C-4alpha-methyl-hydroxylating)
Definition
24-Methylidenecycloartanol + 6 Ferrocytochrome b5 + 3 Oxygen + 6 H+ <=> 3beta-Hydroxy-4beta,14alpha-dimethyl-9beta,19-cyclo-5alpha-ergost-24(24(1))-en-4alpha-carboxylate + 6 Ferricytochrome b5 + 4 H2O
Equation
C08830 + 6 C00999 + 3 C00007 + 6 C00080 <=> C22116 + 6 C00996 + 4 C00001
Comment
three-step reaction (see R12368+R12369+R12370)
Reaction class
RC01952  C08830_C22116
Enzyme
1.14.18.10
Pathway
rn00100  Steroid biosynthesis
rn01100  Metabolic pathways
rn01110  Biosynthesis of secondary metabolites
Module
M00917  Phytosterol biosynthesis, squalene 2,3-epoxide => campesterol/sitosterol
Brite
Enzymatic reactions [BR:br08201]
 1. Oxidoreductase reactions
  1.14  Acting on paired donors, with incorporation or reduction of molecular oxygen
   1.14.18  With another compound as one donor, and incorporation of one atom of oxygen into the other donor
    1.14.18.10
     R12377  24-Methylidenecycloartanol + 6 Ferrocytochrome b5 + 3 Oxygen + 6 H+ <=> 3beta-Hydroxy-4beta,14alpha-dimethyl-9beta,19-cyclo-5alpha-ergost-24(24(1))-en-4alpha-carboxylate + 6 Ferricytochrome b5 + 4 H2O
Orthology
K14423  plant 4,4-dimethylsterol C-4alpha-methyl-monooxygenase [EC:1.14.18.10]
LinkDB

KEGG   REACTION: R12368
Entry
R12368                      Reaction                               
Name
24-methylidenecycloartanol,ferrocytochrome-b5:oxygen oxidoreductase
Definition
24-Methylidenecycloartanol + 2 Ferrocytochrome b5 + Oxygen + 2 H+ <=> 4alpha-(Hydroxymethyl)-4beta,14alpha-dimethyl-9beta,19-cyclo-5alpha-ergost-24(24(1))-en-3beta-ol + 2 Ferricytochrome b5 + H2O
Equation
C08830 + 2 C00999 + C00007 + 2 C00080 <=> C22114 + 2 C00996 + C00001
Comment
the first step of three-step reaction (see R12377, R12368+R12369+R12370)
Reaction class
RC00257  C08830_C22114
Enzyme
1.14.18.10
Brite
Enzymatic reactions [BR:br08201]
 1. Oxidoreductase reactions
  1.14  Acting on paired donors, with incorporation or reduction of molecular oxygen
   1.14.18  With another compound as one donor, and incorporation of one atom of oxygen into the other donor
    1.14.18.10
     R12368  24-Methylidenecycloartanol + 2 Ferrocytochrome b5 + Oxygen + 2 H+ <=> 4alpha-(Hydroxymethyl)-4beta,14alpha-dimethyl-9beta,19-cyclo-5alpha-ergost-24(24(1))-en-3beta-ol + 2 Ferricytochrome b5 + H2O
LinkDB

KEGG   REACTION: R12369
Entry
R12369                      Reaction                               
Name
4alpha-(hydroxymethyl)-4beta,14alpha-dimethyl-9beta,19-cyclo-5alpha-ergost-24(24(1))-en-3beta-ol,ferrocytochrome-b5:oxygen oxidoreductase
Definition
4alpha-(Hydroxymethyl)-4beta,14alpha-dimethyl-9beta,19-cyclo-5alpha-ergost-24(24(1))-en-3beta-ol + 2 Ferrocytochrome b5 + Oxygen + 2 H+ <=> 4alpha-Formyl-4beta,14alpha-dimethyl-9beta,19-cyclo-5alpha-ergost-24(24(1))-en-3beta-ol + 2 Ferricytochrome b5 + 2 H2O
Equation
C22114 + 2 C00999 + C00007 + 2 C00080 <=> C22115 + 2 C00996 + 2 C00001
Comment
the second step of three-step reaction (see R12377, R12368+R12369+R12370)
Reaction class
RC00893  C22114_C22115
Enzyme
1.14.18.10
Brite
Enzymatic reactions [BR:br08201]
 1. Oxidoreductase reactions
  1.14  Acting on paired donors, with incorporation or reduction of molecular oxygen
   1.14.18  With another compound as one donor, and incorporation of one atom of oxygen into the other donor
    1.14.18.10
     R12369  4alpha-(Hydroxymethyl)-4beta,14alpha-dimethyl-9beta,19-cyclo-5alpha-ergost-24(24(1))-en-3beta-ol + 2 Ferrocytochrome b5 + Oxygen + 2 H+ <=> 4alpha-Formyl-4beta,14alpha-dimethyl-9beta,19-cyclo-5alpha-ergost-24(24(1))-en-3beta-ol + 2 Ferricytochrome b5 + 2 H2O
LinkDB

KEGG   REACTION: R12370
Entry
R12370                      Reaction                               
Name
4alpha-formyl-4beta,14alpha-dimethyl-9beta,19-cyclo-5alpha-ergost-24(24(1))-en-3beta-ol,ferrocytochrome-b5:oxygen oxidoreductase
Definition
4alpha-Formyl-4beta,14alpha-dimethyl-9beta,19-cyclo-5alpha-ergost-24(24(1))-en-3beta-ol + 2 Ferrocytochrome b5 + Oxygen + 2 H+ <=> 3beta-Hydroxy-4beta,14alpha-dimethyl-9beta,19-cyclo-5alpha-ergost-24(24(1))-en-4alpha-carboxylate + 2 Ferricytochrome b5 + H2O
Equation
C22115 + 2 C00999 + C00007 + 2 C00080 <=> C22116 + 2 C00996 + C00001
Comment
the last step of three-step reaction (see R12377, R12368+R12369+R12370)
Reaction class
RC01563  C22115_C22116
Enzyme
1.14.18.10
Brite
Enzymatic reactions [BR:br08201]
 1. Oxidoreductase reactions
  1.14  Acting on paired donors, with incorporation or reduction of molecular oxygen
   1.14.18  With another compound as one donor, and incorporation of one atom of oxygen into the other donor
    1.14.18.10
     R12370  4alpha-Formyl-4beta,14alpha-dimethyl-9beta,19-cyclo-5alpha-ergost-24(24(1))-en-3beta-ol + 2 Ferrocytochrome b5 + Oxygen + 2 H+ <=> 3beta-Hydroxy-4beta,14alpha-dimethyl-9beta,19-cyclo-5alpha-ergost-24(24(1))-en-4alpha-carboxylate + 2 Ferricytochrome b5 + H2O
LinkDB

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