Cupriavidus cauae: KZ686_14235 Help Entry KZ686_14235       CDS       T08639                                  Symbol leuD Name (GenBank) 3-isopropylmalate dehydratase small subunit   KO K01704   3-isopropylmalate/(R)-2-methylmalate dehydratase small subunit [EC:4.2.1.33 4.2.1.35] Organism ccax  Cupriavidus cauae Pathway ccax00290   Valine, leucine and isoleucine biosynthesis ccax00660   C5-Branched dibasic acid metabolism ccax01100   Metabolic pathways ccax01110   Biosynthesis of secondary metabolites ccax01210   2-Oxocarboxylic acid metabolism ccax01230   Biosynthesis of amino acids Module ccax_M00432   Leucine biosynthesis, 2-oxoisovalerate => 2-oxoisocaproate Brite KEGG Orthology (KO) [BR:ccax00001]  09100 Metabolism   09101 Carbohydrate metabolism    00660 C5-Branched dibasic acid metabolism     KZ686_14235 (leuD)   09105 Amino acid metabolism    00290 Valine, leucine and isoleucine biosynthesis     KZ686_14235 (leuD) Enzymes [BR:ccax01000]  4. Lyases   4.2  Carbon-oxygen lyases    4.2.1  Hydro-lyases     4.2.1.33  3-isopropylmalate dehydratase      KZ686_14235 (leuD)     4.2.1.35  (R)-2-methylmalate dehydratase      KZ686_14235 (leuD) BRITE hierarchy SSDB OrthologParalogGene clusterGFIT Motif Pfam:  Aconitase_C Motif Other DBs NCBI-ProteinID:  UZN48878 LinkDB All DBs Position 1:complement(3201255..3201905) Genome browser AA seq 216 aa AA seqDB search MEKFTVHKGLVAPLDRANVDTDAIIPKQFLKSIKRTGFGPNLFDEWRYLDVGQPGQDNSK RPLNPDFVLNQPRYQGASILLARENFGCGSSREHAPWALTQYGFRAVIAPSFADIFFNNC YKNGLLPVVLTAAQVDQLFNETNAFNGYQLTIDLERQAVITPSGAGFEFDIAPFRKYCML NGFDDIGLTLRHADKIRAFEAERVTKMPWLNNRLVG NT seq 651 nt NT seq  +upstreamnt  +downstreamnt atggaaaagttcactgtacacaagggcctggtggcgccgctcgatcgtgccaacgtcgat accgacgcgatcatcccgaagcaattcctcaagtcgatcaagcgcacgggcttcggcccg aacctgttcgacgaatggcgctatctcgatgtcggccagcccggccaggacaacagcaag cgcccgctgaacccggacttcgtgctgaaccagccgcgctaccagggcgcgtcgatcctg ctggcgcgcgagaacttcggctgcggcagctcccgcgagcatgcgccgtgggcgctgacg cagtacggcttccgcgcggtgatcgcgccgagcttcgccgacatcttcttcaacaactgc tacaagaacggcctgctgccggtggtgctgaccgcggcccaggtcgaccagctgttcaac gagaccaacgccttcaacggctaccagctgaccatcgacctggagcgccaggccgtgatc acgccgtcgggcgcgggcttcgagttcgacatcgcgccgttccgcaagtactgcatgctg aacggcttcgacgatatcggcctgacgctgcgccacgcggacaagatccgcgccttcgag gccgagcgcgtgacgaagatgccgtggctgaacaaccggctggtcggctga

Cupriavidus cauae: KZ686_14240 Help Entry KZ686_14240       CDS       T08639                                  Symbol leuC Name (GenBank) 3-isopropylmalate dehydratase large subunit   KO K01703   3-isopropylmalate/(R)-2-methylmalate dehydratase large subunit [EC:4.2.1.33 4.2.1.35] Organism ccax  Cupriavidus cauae Pathway ccax00290   Valine, leucine and isoleucine biosynthesis ccax00660   C5-Branched dibasic acid metabolism ccax01100   Metabolic pathways ccax01110   Biosynthesis of secondary metabolites ccax01210   2-Oxocarboxylic acid metabolism ccax01230   Biosynthesis of amino acids Module ccax_M00432   Leucine biosynthesis, 2-oxoisovalerate => 2-oxoisocaproate Brite KEGG Orthology (KO) [BR:ccax00001]  09100 Metabolism   09101 Carbohydrate metabolism    00660 C5-Branched dibasic acid metabolism     KZ686_14240 (leuC)   09105 Amino acid metabolism    00290 Valine, leucine and isoleucine biosynthesis     KZ686_14240 (leuC) Enzymes [BR:ccax01000]  4. Lyases   4.2  Carbon-oxygen lyases    4.2.1  Hydro-lyases     4.2.1.33  3-isopropylmalate dehydratase      KZ686_14240 (leuC)     4.2.1.35  (R)-2-methylmalate dehydratase      KZ686_14240 (leuC) BRITE hierarchy SSDB OrthologParalogGene clusterGFIT Motif Pfam:  Aconitase Motif Other DBs NCBI-ProteinID:  UZN48879 LinkDB All DBs Position 1:complement(3201939..3203348) Genome browser AA seq 469 aa AA seqDB search MAKTLYDKLWDDHTVHVEEDGTTLLYIDRHLLHEVTSPQAFEGLKLAERPVWRISANLAV SDHNVPTTDRSHGIADPISRLQVDTLDANCDAYGITQFKMNDHRQGIVHVIGPEQGATLP GMTVVCGDSHTSTHGAFGALAHGIGTSEVEHVLATQTLLGKKAKNMLVKVEGKLPRGCTA KDIVLSIIGKIGTAGGTGYTIEFAGSAIRDLTMEGRMTVCNMAIEAGARAGLIAVDDVTL EYVKGRPYAPQGAEWEQAVAYWRTLHSDPGAHFDRVVELRAEEVRPQVTWGTSPEMVISI EDRIPDPDKEKDPNKRNAIERALQYMGLQPNVPIQDVRIDKVFIGSCTNSRIEDMRAAAW VVQKLGRRIAPNVKLAMVVPGSGLVKEQAEREGLDKVFKAAGFEWREPGCSMCLAMNADR LDPGERCASTSNRNFEGRQGAGGRTHLVSPAMAAAAAIEGHFVDIRQLG NT seq 1410 nt NT seq  +upstreamnt  +downstreamnt atggccaagacgctctacgacaaactctgggacgaccataccgtgcacgtcgaggaagac ggcaccacgctgctctatatcgaccggcacctgctgcacgaggtgaccagcccgcaggcg ttcgaagggctgaagctggccgagcggccggtgtggcgcatcagcgcgaacctggcggtg tccgaccacaacgtgccgaccaccgaccgcagccacggcatcgccgatccgatctccagg ctgcaggtcgacacgctggacgccaactgcgacgcctacggcatcacgcagttcaagatg aacgaccaccgccagggcatcgtgcacgtgatcggaccggagcagggcgcgacgctgccg ggcatgacggtggtctgcggcgactcgcacaccagcacgcacggcgccttcggcgcgctg gcgcacggcatcggcacctccgaggtcgagcacgtgctggccacgcagacgctgctgggc aagaaggcgaagaacatgctggtcaaggtcgagggcaagctgccgcgcggctgcaccgcc aaggacatcgtgctgtccatcatcggcaagatcggcaccgcgggcggtaccggctacacc atcgagttcgccggctcggcgatccgcgacctgaccatggaagggcgcatgacggtctgc aacatggcgatcgaagcgggcgcgcgcgccggcctgatcgccgtcgacgacgtcacgctg gaatacgtcaagggccgtccctacgcgccgcagggcgccgaatgggagcaggcggtcgcc tactggcgcacgctgcattcggacccgggcgcgcatttcgaccgcgtggtcgagctgcgt gccgaggaagtgcgtccgcaggtcacctggggcacctcgcccgaaatggtgatcagcatc gaggaccgcattcccgatccggacaaggaaaaggacccgaacaagcgcaacgcgatcgaa cgcgcgctgcagtacatgggcctgcagccgaacgtgccgatccaggacgtgcgcatcgac aaggtgttcatcggctcgtgcaccaacagccggatcgaggacatgcgcgccgccgcctgg gtggtgcagaagctgggccgccgcatcgccccgaacgtcaagctggcgatggtggtgccg ggctcgggcctggtcaaggaacaggccgagcgcgaagggctggacaaggtcttcaaggcc gccggcttcgaatggcgcgagccgggctgctcgatgtgcctggcgatgaatgccgaccgg ctcgatccgggcgagcgctgcgcctcgacctcgaaccgcaatttcgagggccggcagggc gccggcggccgcacgcatctggtcagcccggcgatggccgcggcggccgcgatcgaaggc cattttgtcgatatccgccagctcggctga

Cupriavidus cauae: KZ686_18675 Help Entry KZ686_18675       CDS       T08639                                  Symbol leuD Name (GenBank) 3-isopropylmalate dehydratase small subunit   KO K01704   3-isopropylmalate/(R)-2-methylmalate dehydratase small subunit [EC:4.2.1.33 4.2.1.35] Organism ccax  Cupriavidus cauae Pathway ccax00290   Valine, leucine and isoleucine biosynthesis ccax00660   C5-Branched dibasic acid metabolism ccax01100   Metabolic pathways ccax01110   Biosynthesis of secondary metabolites ccax01210   2-Oxocarboxylic acid metabolism ccax01230   Biosynthesis of amino acids Module ccax_M00432   Leucine biosynthesis, 2-oxoisovalerate => 2-oxoisocaproate Brite KEGG Orthology (KO) [BR:ccax00001]  09100 Metabolism   09101 Carbohydrate metabolism    00660 C5-Branched dibasic acid metabolism     KZ686_18675 (leuD)   09105 Amino acid metabolism    00290 Valine, leucine and isoleucine biosynthesis     KZ686_18675 (leuD) Enzymes [BR:ccax01000]  4. Lyases   4.2  Carbon-oxygen lyases    4.2.1  Hydro-lyases     4.2.1.33  3-isopropylmalate dehydratase      KZ686_18675 (leuD)     4.2.1.35  (R)-2-methylmalate dehydratase      KZ686_18675 (leuD) BRITE hierarchy SSDB OrthologParalogGene clusterGFIT Motif Pfam:  Aconitase_C Motif Other DBs NCBI-ProteinID:  UZN52117 LinkDB All DBs Position 2:complement(731183..731833) Genome browser AA seq 216 aa AA seqDB search MTETLRIDGKAAAIRIENLDTDQIMPKQFLRGIDKSGLAQGLLYDLRFDAKGEPRADFVL NRPQYAGTSILIGGSNFGCGSSREHAVWGLQQYGIRAVIAPSFGEIFYSNAMNNRLLLVM LSPEQVAALMEIVEASDGGPVGIDIEAMRLHAEGLDAGFTLSARHREMFLQGLDIIGLSL AYRQQIEAFAERRWAAEPWTRDIAAVTRRRLGGSAG NT seq 651 nt NT seq  +upstreamnt  +downstreamnt atgactgaaacgcttcgtatcgacggcaaggccgcggcgatccgcatcgagaacctggac accgaccagatcatgccgaagcagttcctgcgcggcatcgacaaatcggggctggcgcaa gggctgctgtacgacctgcgcttcgacgcgaagggcgagccgcgcgccgacttcgtgctc aatcgcccgcaatacgccggcacgtcgatcctgatcggcggctccaacttcggttgcggc tcgagccgcgagcatgcggtctggggtttgcagcagtacggcatccgcgcggtgatcgcg ccgagcttcggcgagatcttctattcgaacgcgatgaacaacaggctgctgctggtgatg ctgtcgccggagcaggtcgccgcgctgatggagatcgtcgaggccagcgacggtggtccg gtcggcatcgacatcgaagcgatgcggctgcacgccgagggcctcgatgccggctttacg ctgtcggcgcgccatcgcgagatgttcctgcaggggctggacatcatcggcctgtcgctg gcgtatcggcagcaaatcgaggccttcgccgagcggcgctgggccgccgagccttggacg cgcgacatcgccgcggtgacgcgccgacggctcggcggtagcgccgggtaa

Cupriavidus cauae: KZ686_18680 Help Entry KZ686_18680       CDS       T08639                                  Symbol leuC Name (GenBank) 3-isopropylmalate dehydratase large subunit   KO K01703   3-isopropylmalate/(R)-2-methylmalate dehydratase large subunit [EC:4.2.1.33 4.2.1.35] Organism ccax  Cupriavidus cauae Pathway ccax00290   Valine, leucine and isoleucine biosynthesis ccax00660   C5-Branched dibasic acid metabolism ccax01100   Metabolic pathways ccax01110   Biosynthesis of secondary metabolites ccax01210   2-Oxocarboxylic acid metabolism ccax01230   Biosynthesis of amino acids Module ccax_M00432   Leucine biosynthesis, 2-oxoisovalerate => 2-oxoisocaproate Brite KEGG Orthology (KO) [BR:ccax00001]  09100 Metabolism   09101 Carbohydrate metabolism    00660 C5-Branched dibasic acid metabolism     KZ686_18680 (leuC)   09105 Amino acid metabolism    00290 Valine, leucine and isoleucine biosynthesis     KZ686_18680 (leuC) Enzymes [BR:ccax01000]  4. Lyases   4.2  Carbon-oxygen lyases    4.2.1  Hydro-lyases     4.2.1.33  3-isopropylmalate dehydratase      KZ686_18680 (leuC)     4.2.1.35  (R)-2-methylmalate dehydratase      KZ686_18680 (leuC) BRITE hierarchy SSDB OrthologParalogGene clusterGFIT Motif Pfam:  Aconitase AcnX Motif Other DBs NCBI-ProteinID:  UZN52118 LinkDB All DBs Position 2:complement(731826..733244) Genome browser AA seq 472 aa AA seqDB search MIPRTLYRKLVDSHTVARLDDDNVLLFCDLHLMNEYTSPQAFAGLHEQGRPVLMPGQNVA VVSHIIPTHPGAIRVIADPASALQASNLKANCDRHGIALFDTNDRLQGIEHVVAPEHGMI RPGMVVICGDSHTTTYGALGALGFGIGTSEVEHVLATQTLVYRLAKDMRIRVDGTLPAGT TAKDLVLMIIGRIGAQGARGYVVEFCGSAIESLSVEARFTLCNMAVEAGARGALIAPDAT AIEYVLQRAPDIDEELQPQALAAWRALRSDADAAFDVEHRFDASDVAPHVTWGTSPDQVV PVGGRIPDPLEQPEGPARRSTEQALSYTRLEPRAPIAGTPIQHVFIGSCTNARIEDLRAV AQVVRGRRVADGVRAMVVPGSGAVKAQAEAEGIAALLIDAGFEWRQPGCSMCLAMNDDVL EAGVRCASTTNRNFEGRQGRGAITHLMSPAMAAAAAVTGRITDVRALERHHD NT seq 1419 nt NT seq  +upstreamnt  +downstreamnt atgattccccgcacgctatatcgcaagctggtcgattcgcatacggtcgccagactggac gacgacaacgtcctgctgttctgcgatctgcatctgatgaacgagtacaccagcccgcag gccttcgccggcctgcacgagcagggccggccggtgctgatgccggggcagaacgtcgcc gtggtcagccatatcatcccgacgcatcccggcgcgatccgcgtgattgccgaccctgcg tcggccctgcaggcgagcaacctgaaggccaactgcgaccggcacgggattgcgctgttc gataccaacgacaggctgcagggcatcgagcatgtggtcgcgcccgagcacggcatgatc cggcccggcatggtggtgatctgcggcgacagccataccaccacctatggcgcgctgggt gcgctcggcttcggcatcggcacctcggaggtcgagcatgtgctggcgacgcagacgctg gtctaccggctggcaaaggacatgcggattcgcgtcgacggcacgctgccggctggcacc acggccaaggacctggtactgatgatcatcggccggatcggcgcgcagggcgcgcgcggc tatgtggtcgagttctgcggcagcgcgatcgaatcgctcagcgtcgaggcccgcttcacg ctgtgcaatatggcggtcgaggccggcgcgcgcggcgcgctgatcgcgcccgacgccacc gccatcgagtacgtgctgcaacgcgcgccggatatcgatgaggaactgcagccgcaggcg cttgccgcctggcgcgcactgcgcagcgatgccgacgcggccttcgacgtcgaacaccgt tttgatgccagcgacgtggcgccccatgtcacctggggcaccagcccggatcaggtcgtg ccggtgggcggacggatccccgatccgctggaacaaccggagggccccgcacgccgcagc accgagcaggcgctgtcctatacgcgtctggagccccgcgcgccgatcgccggcacgccg atccagcacgtattcatcggctcctgcaccaacgcccgcatcgaggacttgcgtgcggtg gcccaggtggtgcgcggccggcgcgtggcggacggcgtacgcgcgatggtggtgcccggc tcgggcgcggtcaaggcgcaggccgaggccgagggcatcgccgcgctgctgatcgacgcc ggcttcgaatggcggcagccgggctgctcgatgtgcctggcgatgaacgacgacgtgctg gaggccggcgtgcgctgcgcctcgaccaccaaccgcaatttcgaaggccggcagggcagg ggggcgatcacccatctgatgagccctgcgatggcggccgccgccgccgtgactggccgc atcaccgatgtacgcgcactggagcgccaccatgactga

Cupriavidus cauae: KZ686_20295 Help Entry KZ686_20295       CDS       T08639                                  Name (GenBank) 3-isopropylmalate dehydratase   KO K01704   3-isopropylmalate/(R)-2-methylmalate dehydratase small subunit [EC:4.2.1.33 4.2.1.35] Organism ccax  Cupriavidus cauae Pathway ccax00290   Valine, leucine and isoleucine biosynthesis ccax00660   C5-Branched dibasic acid metabolism ccax01100   Metabolic pathways ccax01110   Biosynthesis of secondary metabolites ccax01210   2-Oxocarboxylic acid metabolism ccax01230   Biosynthesis of amino acids Module ccax_M00432   Leucine biosynthesis, 2-oxoisovalerate => 2-oxoisocaproate Brite KEGG Orthology (KO) [BR:ccax00001]  09100 Metabolism   09101 Carbohydrate metabolism    00660 C5-Branched dibasic acid metabolism     KZ686_20295   09105 Amino acid metabolism    00290 Valine, leucine and isoleucine biosynthesis     KZ686_20295 Enzymes [BR:ccax01000]  4. Lyases   4.2  Carbon-oxygen lyases    4.2.1  Hydro-lyases     4.2.1.33  3-isopropylmalate dehydratase      KZ686_20295     4.2.1.35  (R)-2-methylmalate dehydratase      KZ686_20295 BRITE hierarchy SSDB OrthologParalogGene clusterGFIT Motif Pfam:  Aconitase_C Motif Other DBs NCBI-ProteinID:  UZN52531 LinkDB All DBs Position 2:complement(1119036..1119512) Genome browser AA seq 158 aa AA seqDB search MWRFGDNVDTDAMAPATQMKGGIEQIARHCLAGLRPEFPATVRPGDLVIGGRHFGVGSSR EQAPQALRHLGVAAVIAPSFAGLFYRNALNLGLPALVCADTARLVDGEHATLDLENAAVV LPDGSRVPCEPIPAFLLDMLRAGGLIPHLKRRLAQQRA NT seq 477 nt NT seq  +upstreamnt  +downstreamnt atctggcgcttcggcgacaacgtcgataccgatgcgatggcgcccgccacgcagatgaaa ggcggcatcgagcagatcgcgcgccattgcctggccggattgcgtcccgaatttcccgcg accgtacggccgggcgatctggtcatcggcggacggcatttcggcgtgggctcgtcgcgc gagcaggctccccaggccttgcgccatctcggcgtggcggcagtgatcgcgccttcgttc gcgggcctgttctatcgcaatgcgttgaatctcggcctgccggcgctggtctgcgcggat accgccagactggtagacggcgaacacgcgaccctggatctggagaacgcggccgtcgtg ctgcccgacggcagccgcgtgccctgcgaaccgatcccggcgttcctgctcgacatgctg cgcgccggcgggctgatcccgcatctgaagcggcggctggcgcagcagcgcgcttga

Cupriavidus cauae: KZ686_20300 Help Entry KZ686_20300       CDS       T08639                                  Name (GenBank) 3-isopropylmalate dehydratase large subunit   KO K01703   3-isopropylmalate/(R)-2-methylmalate dehydratase large subunit [EC:4.2.1.33 4.2.1.35] Organism ccax  Cupriavidus cauae Pathway ccax00290   Valine, leucine and isoleucine biosynthesis ccax00660   C5-Branched dibasic acid metabolism ccax01100   Metabolic pathways ccax01110   Biosynthesis of secondary metabolites ccax01210   2-Oxocarboxylic acid metabolism ccax01230   Biosynthesis of amino acids Module ccax_M00432   Leucine biosynthesis, 2-oxoisovalerate => 2-oxoisocaproate Brite KEGG Orthology (KO) [BR:ccax00001]  09100 Metabolism   09101 Carbohydrate metabolism    00660 C5-Branched dibasic acid metabolism     KZ686_20300   09105 Amino acid metabolism    00290 Valine, leucine and isoleucine biosynthesis     KZ686_20300 Enzymes [BR:ccax01000]  4. Lyases   4.2  Carbon-oxygen lyases    4.2.1  Hydro-lyases     4.2.1.33  3-isopropylmalate dehydratase      KZ686_20300     4.2.1.35  (R)-2-methylmalate dehydratase      KZ686_20300 BRITE hierarchy SSDB OrthologParalogGene clusterGFIT Motif Pfam:  Aconitase AcnX Motif Other DBs NCBI-ProteinID:  UZN52389 LinkDB All DBs Position 2:complement(1119611..1120915) Genome browser AA seq 434 aa AA seqDB search MSRAGAGTAPAQTLAQKLVARAAGLDRATPGTVVTCKVDLAMSHDSSGPRRVAPLLRELG AKVWDPERFVVVTDHFVPAADAEAEAIVRFTRDWVREQRLPHLIDNEGICHVVLPERGYV LPGQCIVGGDSHSPTGGAFGAYMFGIGATEMAGVLATGEIWLRVPQTIRIQWDGTLADGV CAKDIMLFLCARLGLGGGCYEAIEYAGTAVTALSMQERMTLTNMSAELGAQTGLVAPDAT TLQWLEQAGVPAATLAQIDIEHWRSDADAPLLQTHRFDAAALAPQVAAPHSPANGAPVSD AQGQPIDLAYLGACTGAKLDDLRMAARILRGRKVAPGVSLQLAPASLRDQQQAEREGTLA ALVDAGAQLLPNACNACAGYGPTRFAAGTRAIASTARNFAGRMGDAGSAVWLASPMTVAA SAVTGRITDPRTLL NT seq 1305 nt NT seq  +upstreamnt  +downstreamnt atgagccgtgccggcgccgggaccgccccggcgcaaaccctcgcacagaagctggtcgcc cgcgccgcgggcctggaccgcgcgacgcccggcaccgtggtgacctgcaaggtcgatctg gcgatgtcgcacgactccagcggaccgcgccgcgtcgcgccgctgctgcgcgagctgggc gccaaggtctgggaccccgagcgcttcgtcgtcgtgaccgaccacttcgtgcccgccgcg gatgccgaggccgaggcgatcgtccgcttcacgcgcgactgggtccgcgaacagcgcctg ccgcatctgatcgacaacgaagggatctgccacgtggtgctgcccgagcgcggctacgtg ctgccgggccagtgcatcgtcggcggcgacagccacagtccgaccgggggcgcgttcggc gcctatatgttcggcatcggcgccaccgagatggcgggcgtgctggccaccggcgagatc tggctgcgcgtgccgcagaccatccgcatccaatgggacggcacgctggccgacggcgtc tgcgccaaggacatcatgctgttcctgtgcgcccggctcggactgggcggcggctgctac gaggcgatcgaatatgccggcacggcggtgaccgcgctgtcgatgcaggagcggatgacg ctgaccaatatgagcgccgagctgggcgcgcagacgggcctcgtcgcaccggacgccacc acgctccaatggctcgaacaggccggcgtgcccgccgccacgctggcgcagatcgatatc gagcactggcgcagcgatgccgatgcgccgctgctgcaaacccatcgcttcgacgccgcc gccctggcgccgcaggtcgccgcgccgcactcgccggccaacggcgcgccggtcagcgac gcccagggccagccgatcgacctcgcctatctcggcgcctgcacgggcgccaagctcgac gatctgcgcatggccgcgcgcatcctgcgcgggcgcaaggtcgcgccgggcgtctcgctg cagctggcacccgcctcgctgcgcgaccagcagcaggccgagcgcgaaggcacgctggcg gcactcgtcgatgccggcgcgcagctgctgcccaatgcgtgcaatgcctgcgccggttac gggcccacgcgcttcgccgccggcacgcgggcgatcgcctcgaccgcgcgcaatttcgcg ggccggatgggcgacgccggcagcgcggtgtggctggcctcgccgatgaccgtggccgca tcggcggtcacgggccgcatcaccgatccgcgcacgctgctgtga

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