Entry Name O-Antigen repeat unit biosynthesis
Description O-antigen is a repetitive glycan polymer synthesized as the most variable part in the lipopolysaccharide (LPS) biosynthesis (
map00541 ). Structural diversity of O-antigen repeat units results from genetic diversity as summarized here for completely sequenced Escherichia coli genomes, many of which are for pathogenic strains.
Class Metabolism; Glycan biosynthesis and metabolismBRITE hierarchy
Pathway map ko00542 O-Antigen repeat unit biosynthesis
Orthology K02851 wecA, tagO, rfe; UDP-GlcNAc:undecaprenyl-phosphate/decaprenyl-phosphate GlcNAc-1-phosphate transferase [EC:2.7.8.33 2.7.8.35 ]
K12993 wbdA, mtfA; O-antigen biosynthesis alpha-1,2-mannosyltransferase [EC:2.4.1.371 2.4.1.-]
K18788 wbbD; UDP-Gal:alpha-D-GlcNAc-diphosphoundecaprenol beta-1,3-galactosyltransferase [EC:2.4.1.303 ]
K20883 wbgN; O55-antigen biosynthesis alpha-1,2-colitosyltransferase [EC:2.4.1.341 ]
K21365 wbnH, wbiN; O86/O127-antigen biosynthesis alpha-1,3-N-acetylgalactosaminyltransferase [EC:2.4.1.306 ]
K21366 wbnJ, wbiP; O86/O127-antigen biosynthesis beta-1,3-galactosyltransferase [EC:2.4.1.122 2.4.1.-]
K21367 wbnK, wbiQ; O86/O127-antigen biosynthesis alpha-1,2-fucosyltransferase [EC:2.4.1.308 2.4.1.-]
K21368 wbnI; O86-antigen biosynthesis alpha-1,3-galactosyltransferase [EC:2.4.1.309 ]
K21586 wbgM; O55-antigen biosynthesis alpha-1,3-galactosyltransferase
K23072 wbbI; O16-antigen biosynthesis beta-1,6-galactofuranosyltransferase
K23073 wbbK; O16-antigen biosynthesis glucosyltransferase
K23101 wekV; O18-antigen biosynthesis glucosyltransferase
K23102 wekW; O18-antigen biosynthesis alpha-1,3-galactosyltransferase
K23103 wekU; O18-antigen biosynthesis glycosyltransferase
K23104 wbdH; O111-antigen biosynthesis galactosyltransferase
K23105 wbdL; O111-antigen biosynthesis colitosyltransferase
K23130 wbdN; O157-antigen biosynthesis beta-1,3-glucosyltransferase
K23131 wbdO; O157-antigen biosynthesis glycosyltransferase
K23132 wbdP; O157-antigen biosynthesis glycosyltransferase
K23189 wbuB; O26/O145-antigen biosynthesis N-acetyl-L-fucosamine transferase
K23190 wbuA; O26-antigen biosynthesis rhamnosyltransferase
K23191 wbuW; O145-antigen biosynthesis Neu5Ac transferase
K23197 wbbA; O7-antigen biosynthesis N-acetylviosaminyltransferase
K23198 wbbB; O7-antigen biosynthesis rhamnosyltransferase
K23199 wbbC; O7-antigen biosynthesis mannosyltransferase
K23201 wbaC; O17/O44/073/077/0106-antigen biosynthesis mannosyltransferase
K23202 wbaD; O17/O44/073/077/0106-antigen biosynthesis mannosyltransferase
K23229 wbgP; O55-antigen biosynthesis glycosyltransferase
K23230 wbgO; O55-antigen biosynthesis beta-1,3-galactosyltransferase
K23249 wbwA; O104-antigen biosynthesis alpha-2,3-NeuNAc transferase
K23250 wbwB; O104-antigen biosynthesis alpha-1,4-galactosyltransferase
K23251 wbwC; O104-antigen biosynthesis beta-1,3-galactosyltransferase
K24649 wbdM; O111-antigen biosynthesis glucosyltransferase
K24658 wbdA; O-antigen biosynthesis alpha-1,2-mannosyltransferase
K24906 wekT; O18-antigen biosynthesis rhamnosyltransferase
Reference Authors Riley JG, Menggad M, Montoya-Peleaz PJ, Szarek WA, Marolda CL, Valvano MA, Schutzbach JS, Brockhausen I
Title The wbbD gene of E. coli strain VW187 (O7:K1) encodes a UDP-Gal: GlcNAc{alpha}-pyrophosphate-R {beta}1,3-galactosyltransferase involved in the biosynthesis of O7-specific lipopolysaccharide.
Journal Reference Authors Marolda CL, Feldman MF, Valvano MA
Title Genetic organization of the O7-specific lipopolysaccharide biosynthesis cluster of Escherichia coli VW187 (O7:K1).
Journal Reference Authors Greenfield LK, Richards MR, Li J, Wakarchuk WW, Lowary TL, Whitfield C
Title Biosynthesis of the polymannose lipopolysaccharide O-antigens from Escherichia coli serotypes O8 and O9a requires a unique combination of single- and multiple-active site mannosyltransferases.
Journal Reference Authors Wang W, Perepelov AV, Feng L, Shevelev SD, Wang Q, Senchenkova SN, Han W, Li Y, Shashkov AS, Knirel YA, Reeves PR, Wang L
Title A group of Escherichia coli and Salmonella enterica O antigens sharing a common backbone structure.
Journal Reference Authors Stevenson G, Neal B, Liu D, Hobbs M, Packer NH, Batley M, Redmond JW, Lindquist L, Reeves P
Title Structure of the O antigen of Escherichia coli K-12 and the sequence of its rfb gene cluster.
Journal Reference Authors Reeves PR, Hobbs M, Valvano MA, Skurnik M, Whitfield C, Coplin D, Kido N, Klena J, Maskell D, Raetz CR, Rick PD.
Title Bacterial polysaccharide synthesis and gene nomenclature.
Journal Reference Authors Jann B, Shashkov AS, Gupta DS, Jann K.
Title The O18 antigens (lipopolysaccharides) of Escherichia coli. Structural characterization of the O18A, O18A1, O18B and O18B1-specific polysaccharides.
Journal Reference Authors Jansson PE, Kenne L, Widmalm G.
Title Structure of the O-antigen polysaccharide from Escherichia coli O18ac: a revision using computer-assisted structural analysis with the program CASPER.
Journal Reference Authors Li D, Liu B, Chen M, Guo D, Guo X, Liu F, Feng L, Wang L
Title A multiplex PCR method to detect 14 Escherichia coli serogroups associated with urinary tract infections.
Journal Reference Authors Manca MC, Weintraub A, Widmalm G.
Title Structural studies of the Escherichia coli O26 O-antigen polysaccharide.
Journal Reference Authors D'Souza JM, Wang L, Reeves P
Title Sequence of the Escherichia coli O26 O antigen gene cluster and identification of O26 specific genes.
Journal Reference Authors Wang L, Huskic S, Cisterne A, Rothemund D, Reeves PR
Title The O-antigen gene cluster of Escherichia coli O55:H7 and identification of a new UDP-GlcNAc C4 epimerase gene.
Journal Reference Authors Liu XW, Xia C, Li L, Guan WY, Pettit N, Zhang HC, Chen M, Wang PG
Title Characterization and synthetic application of a novel beta1,3-galactosyltransferase from Escherichia coli O55:H7.
Journal Reference Authors Fitzgerald C, Collins M, van Duyne S, Mikoleit M, Brown T, Fields P
Title Multiplex, bead-based suspension array for molecular determination of common Salmonella serogroups.
Journal Reference Authors Liu B, Knirel YA, Feng L, Perepelov AV, Senchenkova SN, Reeves PR, Wang L
Title Structural diversity in Salmonella O antigens and its genetic basis.
Journal Reference Authors Gamian A, Romanowska E, Ulrich J, Defaye J.
Title The structure of the sialic acid-containing Escherichia coli O104 O-specific polysaccharide and its linkage to the core region in lipopolysaccharide.
Journal Reference Authors Wang L, Briggs CE, Rothemund D, Fratamico P, Luchansky JB, Reeves PR
Title Sequence of the E. coli O104 antigen gene cluster and identification of O104 specific genes.
Journal Reference Authors Wang S, Czuchry D, Liu B, Vinnikova AN, Gao Y, Vlahakis JZ, Szarek WA, Wang L, Feng L, Brockhausen I
Title Characterization of two UDP-Gal:GalNAc-diphosphate-lipid beta1,3-galactosyltransferases WbwC from Escherichia coli serotypes O104 and O5.
Journal Reference Authors Czuchry D, Desormeaux P, Stuart M, Jarvis DL, Matta KL, Szarek WA, Brockhausen I
Title Identification and Biochemical Characterization of the Novel alpha2,3-Sialyltransferase WbwA from Pathogenic Escherichia coli Serotype O104.
Journal Reference Authors Czuchry D, Szarek WA, Brockhausen I
Title Identification and biochemical characterization of WbwB, a novel UDP-Gal: Neu5Ac-R alpha1,4-galactosyltransferase from the intestinal pathogen Escherichia coli serotype O104.
Journal Reference Authors Stevenson G, Dieckelmann M, Reeves PR
Title Determination of glycosyltransferase specificities for the Escherichia coli O111 O antigen by a generic approach.
Journal Reference Authors Wang L, Curd H, Qu W, Reeves PR
Title Sequencing of Escherichia coli O111 O-antigen gene cluster and identification of O111-specific genes.
Journal Reference Authors Feng L, Senchenkova SN, Tao J, Shashkov AS, Liu B, Shevelev SD, Reeves PR, Xu J, Knirel YA, Wang L
Title Structural and genetic characterization of enterohemorrhagic Escherichia coli O145 O antigen and development of an O145 serogroup-specific PCR assay.
Journal Reference Authors Gao Y, Liu B, Strum S, Schutzbach JS, Druzhinina TN, Utkina NS, Torgov VI, Danilov LL, Veselovsky VV, Vlahakis JZ, Szarek WA, Wang L, Brockhausen I
Title Biochemical characterization of WbdN, a beta1,3-glucosyltransferase involved in O-antigen synthesis in enterohemorrhagic Escherichia coli O157.
Journal Reference Authors Wang L, Reeves PR
Title Organization of Escherichia coli O157 O antigen gene cluster and identification of its specific genes.
Journal Reference Authors Hong Y, Liu MA, Reeves PR
Title Progress in Our Understanding of Wzx Flippase for Translocation of Bacterial Membrane Lipid-Linked Oligosaccharide.
Journal Reference Authors Liu B, Furevi A, Perepelov AV, Guo X, Cao H, Wang Q, Reeves PR, Knirel YA, Wang L, Widmalm G.
Title Structure and genetics of Escherichia coli O antigens.
Journal
