deacetoxycephalosporin-C synthase;
DAOCS;
penicillin N expandase;
DAOC synthase
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With 2-oxoglutarate as one donor, and the other dehydrogenated
Cantwell C, Beckmann R, Whiteman P, Queener SW, Abraham EP.
Title
Isolation of deacetoxycephalosporin C from fermentation broths of Penicillium chrysogenum transformants: construction of a new fungal biosynthetic pathway.
Enzymes for epimerization of isopenicillin N, ring expansion of penicillin N, and 3'-hydroxylation of deacetoxycephalosporin C. Function, evolution, refolding, and enzyme engineering.
Purification and properties of deacetoxycephalosporin C synthase from recombinant Escherichia coli and its comparison with the native enzyme purified from Streptomyces clavuligerus.
Cephamycin C biosynthesis, aminoadipate + cycteine + valine => cephamycin C
Brite
Enzymatic reactions [BR:br08201]
1. Oxidoreductase reactions
1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
1.14.20 With 2-oxoglutarate as one donor, and the other dehydrogenated
1.14.20.1
R05301 Penicillin N + 2-Oxoglutarate + Oxygen <=> Deacetoxycephalosporin C + Succinate + CO2 + H2O