Entry
Name
3-phenylpropanoate dioxygenase;
HcaA1A2CD;
Hca dioxygenase;
3-phenylpropionate dioxygenase
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of two atoms of oxygen into the other donor
BRITE hierarchy
Sysname
3-phenylpropanoate,NADH:oxygen oxidoreductase (2,3-hydroxylating)
Reaction(IUBMB)
(1) 3-phenylpropanoate + NADH + H+ + O2 = 3-(cis-5,6-dihydroxycyclohexa-1,3-dien-1-yl)propanoate + NAD+ [RN:
R06782 ];
(2) (2E)-3-phenylprop-2-enoate + NADH + H+ + O2 = (2E)-3-(2,3-dihydroxyphenyl)prop-2-enoate + NAD+
Reaction(KEGG)
Substrate
Product
3-(cis-5,6-dihydroxycyclohexa-1,3-dien-1-yl)propanoate [CPD:
C11588 ];
NAD+ [CPD:
C00003 ];
(2E)-3-(2,3-dihydroxyphenyl)prop-2-enoate [CPD:
C12623 ]
Comment
This enzyme catalyses a step in the pathway of phenylpropanoid compounds degradation. It catalyses the insertion of both atoms of molecular oxygen into positions 2 and 3 of the phenyl ring of 3-phenylpropanoate or (2E)-3-phenylprop-2-enoate.
History
EC 1.14.12.19 created 2005, modified 2011
Pathway
ec01120 Microbial metabolism in diverse environments
Orthology
K05708 3-phenylpropionate/trans-cinnamate dioxygenase subunit alpha
K05709 3-phenylpropionate/trans-cinnamate dioxygenase subunit beta
Genes
ECO : b2538(hcaE) b2539(hcaF)
ECJ : JW2522(hcaE) JW2523(hcaF)
ECD : ECDH10B_2705(hcaE) ECDH10B_2706(hcaF)
EBW : BWG_2302(hcaE) BWG_2303(hcaF)
ECOK : ECMDS42_2082(hcaE) ECMDS42_2083(hcaF)
ECOC : C3026_14060 C3026_14065
ECE : Z3809(hcaA1) Z3810(hcaA2)
ECS : ECs_3404(hcaE) ECs_3405(hcaF)
ETW : ECSP_3482(hcaE) ECSP_3483(hcaF)
ELX : CDCO157_3171 CDCO157_3172
EOI : ECO111_3262(hcaE) ECO111_3263(hcaF)
EOJ : ECO26_3585(hcaE) ECO26_3586(hcaF)
ECOO : ECRM13514_3370(hcaA1) ECRM13514_3371(hcaA2)
ECOH : ECRM13516_3226(hcaA1) ECRM13516_3227(hcaA2)
ECK : EC55989_2824(hcaE) EC55989_2825(hcaF)
EOK : G2583_3068(hcaE) G2583_3069(hcaF)
ELR : ECO55CA74_15185 ECO55CA74_15190
ECW : EcE24377A_2824(hcaF)
EUN : UMNK88_3191 UMNK88_3192
ECOA : APECO78_16420 APECO78_16425
ECX : EcHS_A2690(hcaE) EcHS_A2691(hcaF)
ECM : EcSMS35_2691(hcaE) EcSMS35_2692(hcaF)
ECR : ECIAI1_2590(hcaE) ECIAI1_2591(hcaF)
EUM : ECUMN_2858(hcaE) ECUMN_2859(hcaF)
ECT : ECIAI39_2739(hcaE) ECIAI39_2740(hcaF)
EOC : CE10_2970(hcaE) CE10_2971(hcaF)
EBR : ECB_02430(hcaE) ECB_02431(hcaF)
ECOB : C3029_16780 C3029_16785
EBL : ECD_02430(hcaE) ECD_02431(hcaF)
EBE : B21_02394(hcaE) B21_02395(hcaF)
ELO : EC042_2742(hcaE) EC042_2743(hcaF)
ECL : EcolC_1138 EcolC_1139
EKO : EKO11_1194 EKO11_1195
EKF : KO11_10125(hcaF) KO11_10130(hcaE)
EDH : EcDH1_1129 EcDH1_1130
EDJ : ECDH1ME8569_2465(hcaE) ECDH1ME8569_2466(hcaF)
ELW : ECW_m2764(hcaE) ECW_m2765(hcaF)
ELL : WFL_13535(hcaE) WFL_13540(hcaF)
ELP : P12B_c2638(hcaA) P12B_c2639(hcaF)
ECOL : LY180_13020 LY180_13025
ESZ : FEM44_02680 FEM44_02685
SFL : SF2585(hcaA1) SF2586(hcaA2)
SFX : S2757(hcaA1) S2758(hcaA2)
SFV : SFV_2586(hcaA1) SFV_2587(hcaA2)
SFE : SFxv_2841(hcaE) SFxv_2842(hcaF)
SFT : NCTC1_02841(hcaA1) NCTC1_02842(hcaA2)
SSN : SSON_2620(hcaA1) SSON_2621(hcaA2)
SBO : SBO_2562(hcaA1) SBO_2563(hcaA2)
SHQ : A0259_16415 A0259_16420
KPV : KPNIH29_19720 KPNIH29_19725
REE : electrica_01232(hcaF) electrica_01233(hcaE)
CFD : CFNIH1_24125 CFNIH1_24130
CWE : CO701_05135 CO701_05140
CYO : CD187_06590 CD187_06595
CPOT : FOB25_17735 FOB25_17740
CFQ : C2U38_18460 C2U38_18465
CIF : AL515_17210 AL515_17215
CIE : AN232_10850 AN232_10855
CPAR : CUC49_13135 CUC49_13140
CITZ : E4Z61_23090 E4Z61_23095
CARS : E1B03_19710 E1B03_19715
CIX : M4I31_06400 M4I31_06405
CIB : HF677_006320 HF677_006325
CITR : GUC46_06305 GUC46_06310
KAS : KATP_11300(hcaF) KATP_11310(hcaE)
KLU : K7B04_23360 K7B04_23365
KCY : RIN60_06205 RIN60_06210
PSGC : G163CM_01200(hcaF) G163CM_01210(hcaE)
SFW : WN53_15140 WN53_15145
SFG : AV650_10680 AV650_10685
PLU : plu2204(hcaE) plu2205(hcaF)
PLUM : A4R40_11050 A4R40_11055
PAY : PAU_02356(hcaF) PAU_02357(hcaE)
PTT : VY86_20035 VY86_20040
PAKH : B0X70_11365 B0X70_11370
PLUI : CE143_11280 CE143_11285
LRI : NCTC12151_03202(hcaF) NCTC12151_03204(hcaE_2)
VNA : PN96_06440 PN96_06445
VEJ : VEJY3_06605 VEJY3_06610
VFU : vfu_A01965 vfu_A01966
PKM : PZ739_18205 PZ739_18210
ALC : OTEC02_08840 OTEC02_08845
AEI : AOY20_01945 AOY20_01950
ASJ : AsACE_CH00358(hcaE) AsACE_CH00359(hcaF)
ADV : DJ533_16925 DJ533_16930
ARJ : DOM24_01605 DOM24_01610
AWU : BEN71_02735 BEN71_02740
ACUM : C9E88_006035 C9E88_006040
ALW : FOB21_11740 FOB21_11745
ATN : FM020_13950 FM020_13955
ACHI : CDG60_02990 CDG60_02995
ALJ : G8D99_13375 G8D99_13380
AWG : GFH30_01735 GFH30_01740
ASHA : G8E00_02275 G8E00_02280
ATG : J4G44_04415 J4G44_04420
AVB : RYU24_27080(hcaF) RYU24_27090(hcaE)
ABOU : ACBO_27680(hcaF) ACBO_27690(hcaE)
ACIU : A3K93_01290 A3K93_01295
APOT : MZO21_10815 MZO21_10820
ATZ : M5E07_14160 M5E07_14165
ACIE : KIP84_08210 KIP84_08215
ACIG : BS636_03570 BS636_03575
AQL : BXU06_16230 BXU06_16235
BDF : WI26_30250 WI26_30255
BANN : JFN94_18365 JFN94_26755 JFN94_26760
PACP : FAZ97_23015 FAZ97_23020
PPAI : E1956_24355 E1956_24360
PHUN : U0042_13205(hcaF) U0042_13210(hcaE)
BOJ : CBF45_10090 CBF45_10095
AALN : Q3V95_01615 Q3V95_01620
AFA : UZ73_04210 UZ73_04215
AAQU : D3M96_14775 D3M96_14780
AAMM : FE795_12870 FE795_12875
ALCN : MID00_13035 MID00_13040
KGY : EHF36_10490 EHF36_10495
DIH : G7047_02060 G7047_02065
AMYL : QBD29_07695 QBD29_07700
NHUM : PQ457_20575 PQ457_20580
MYX : QEG98_21635 QEG98_21640
MSA : Mycsm_01912 Mycsm_01988
MMAT : MMAGJ_45330(hcaE_2)
CAZ : CARG_03210 CARG_03270
CLIZ : G7Y31_11590 G7Y31_11615
CPSL : KBP54_00405 KBP54_01750
CHIN : J5O04_05660 J5O04_05700
CCAW : CCANI_11855(hcaE1) CCANI_11895(hcaE2)
CFEU : CFELI_14015(hcaE1) CFELI_14055(hcaE2)
CORN : BJP05_09105 BJP05_10355
NFR : ERS450000_00303(hcaE_2)
RHOU : KZJ41_10510 KZJ41_10515
RHOW : A4U64_15230 A4U64_15235
ARHD : VSH64_21265 VSH64_21270 VSH64_25915
» show all
Taxonomy
Reference
Authors
Diaz E, Ferrandez A, Garcia JL.
Title
Characterization of the hca cluster encoding the dioxygenolytic pathway for initial catabolism of 3-phenylpropionic acid in Escherichia coli K-12.
Journal
Sequence
Reference
Authors
Burlingame R, Chapman PJ.
Title
Catabolism of phenylpropionic acid and its 3-hydroxy derivative by Escherichia coli.
Journal
Other DBs
UM-BBD (Biocatalysis/Biodegradation Database): 1.14.12.19
LinkDB
All DBs