KEGG   ENZYME: 1.14.13.172
Entry
EC 1.14.13.172              Enzyme                                 
Name
salicylate 5-hydroxylase;
nagG (gene name);
nagH (gene name)
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
Sysname
salicylate,NADH:oxygen oxidoreductase (5-hydroxylating)
Reaction(IUBMB)
salicylate + NADH + H+ + O2 = 2,5-dihydroxybenzoate + NAD+ + H2O [RN:R07709]
Reaction(KEGG)
R07709;
(other) R07710
Substrate
salicylate [CPD:C00805];
NADH [CPD:C00004];
H+ [CPD:C00080];
O2 [CPD:C00007]
Product
2,5-dihydroxybenzoate [CPD:C00628];
NAD+ [CPD:C00003];
H2O [CPD:C00001]
Comment
This enzyme, which was characterized from the bacterium Ralstonia sp. U2, comprises a multicomponent system, containing a reductase that is an iron-sulfur flavoprotein (FAD; EC 1.18.1.7, ferredoxin---NAD(P)+ reductase), an iron-sulfur oxygenase, and ferredoxin.
History
EC 1.14.13.172 created 2013
Pathway
ec00626  Naphthalene degradation
ec01100  Metabolic pathways
ec01120  Microbial metabolism in diverse environments
Orthology
K18242  salicylate 5-hydroxylase large subunit
K18243  salicylate 5-hydroxylase small subunit
Genes
EBTEBL_c34270 EBL_c34280
KASKATP_22590 KATP_22600
PLUplu1000 plu1001
PLUMA4R40_04995 A4R40_05000
PTTVY86_00150 VY86_00155
ALTambt_18005 ambt_18010
TTUTERTU_1474 TERTU_1475
CYQQ91_0876 Q91_2234
CZACYCME_2552
CYYCPC19_01630
MMWMmwyl1_2669 Mmwyl1_2670
MMEMarme_2404 Marme_2405
MPCMar181_2357 Mar181_2358
MPRIMP3633_2253 MP3633_2254
FCEJN531_005580 JN531_005585
GBIPG2T_03615
RSORSc1089(nagH) RSc1090(nagG)
RSLRPSI07_2301 RPSI07_2302
RSNRSPO_c02274 RSPO_c02275(nagH)
RSMCMR15_20208 CMR15_20209
RSEF504_1060(ohbA) F504_1061(ohbB)
RSYRSUY_15110 RSUY_15120
RPJN234_28705 N234_28710
RMNTK49_07930 TK49_07935
RPUCDC45_05590 CDC45_05595
RSGJK151_19135 JK151_19140
REHH16_A1632(nagH) H16_A1633(nagG)
CNCCNE_1c16400(nagH) CNE_1c16410(nagG)
CUHBJN34_29220 BJN34_29225
REUReut_B4719 Reut_B4720
RMERmet_3712(nagH)
CBWRR42_s2751 RR42_s2752
CCUPBKK81_31195 BKK81_31200
CUPBKK80_25150 BKK80_25155
CUUBKK79_30860 BKK79_30865
COXE0W60_10140 E0W60_10145
BMKDM80_1440 DM80_1441
BCEPAPZ15_32865 APZ15_32870
BPYRABD05_24780 ABD05_24785
BUBBW23_5815 BW23_5816
BGLbglu_2g20110 bglu_2g20120
BGUKS03_3913 KS03_3914
BUGBC1001_0576 BC1001_0577
BUQAC233_10900 AC233_10905
BURKDM992_22160 DM992_22165
BPXBUPH_04466 BUPH_04467
BUZAYM40_33040 AYM40_33045 AYM40_33150 AYM40_33155
BFNOI25_3046 OI25_3047
PARBCJU94_38670 CJU94_38675
PTERC2L65_39070 C2L65_39075
PMEGFNZ07_23540 FNZ07_23545 FNZ07_29070 FNZ07_29075
PPKU875_04295 U875_04300
PPNODA70_21370 DA70_21375
PPNMLV28_08945 LV28_08950
PRBX636_05145 X636_05150
PPULRO07_03175 RO07_03180
PSPUNA29_24085 NA29_24090
PAPISG18_03365 SG18_03370
PVEUC34_19330 UC34_19335
POXMB84_24085 MB84_24090
PFGAB870_06325 AB870_06330
PNRAT302_02760 AT302_02765
PANDDRB87_17855 DRB87_17860
PFIBPI93_008710 PI93_008715
BPTBpet2804 Bpet2805
AXYAXYL_00938 AXYL_00939 AXYL_06578 AXYL_06581(bphA)
AXONH44784_011911 NH44784_011921
AXNAX27061_0965 AX27061_0966
AXXERS451415_00956(andAd_1) ERS451415_00957(andAc_1)
ADTAPT56_05010 APT56_05015
AISBUW96_21015 BUW96_21020
ASWCVS48_01680 CVS48_01685
ACHRC2U31_09370
ACHBDVB37_04180 DVB37_04185
ADYHLG70_17185 HLG70_17190
PUDG9Q38_01795 G9Q38_01800 G9Q38_05245 G9Q38_05250
PIGEGT29_23975 EGT29_23980
PARKLSG25_18560 LSG25_18565
RHFEUB48_13715 EUB48_13720
POLBpro_0983 Bpro_0984
PNAPnap_2462 Pnap_2487 Pnap_2488
POSDT070_14060 DT070_14065
POOF7R28_16540 F7R28_16545
AAVAave_0165 Aave_0166
AJSAjs_3090
AAAAcav_0196 Acav_0197
ACKC380_09995 C380_10000
VAPVapar_5348 Vapar_5349
VPEVarpa_2933 Varpa_2934
VPDVAPA_1c07030 VAPA_1c07040 VAPA_1c20400 VAPA_1c20410
VBOCKY39_17840 CKY39_17845
VAMC4F17_05690 C4F17_05695
RTARta_25980 Rta_25990
RTEAHK414_18880 HK414_18885
LIML103DPR2_01957(nagH) L103DPR2_01958(nagG_2)
LIHL63ED372_02714(nagH) L63ED372_02715(nagG_2)
HYRBSY239_1027 BSY239_1029
HYBQ5W_19150 Q5W_19155
HYLLPB072_20440 LPB072_20445
HPSEHPF_02890(nagG) HPF_02895(nagH)
HYNF9K07_14335 F9K07_14340
HTNKI616_03135 KI616_03140
JAJEKL02_16425 EKL02_16430
MASWAM586_27630 AM586_27635 AM586_27695 AM586_27700 AM586_27790 AM586_27795
LCHLcho_0796 Lcho_0797
AZOazo2515(nagH) azo2516(nagG)
AOAdqs_2672 dqs_2673
AZRCJ010_15880 CJ010_15885
CFHC1707_18700 C1707_18705
BPONIFE19_06475 IFE19_06480
NARSaro_3834
NPPPP1Y_AT15862
SWISwit_0895 Swit_3056 Swit_3057
SPHDHY78_10290 HY78_10295 HY78_22215
SBARH5V43_22430
BLASBSY18_3955 BSY18_3956
PALGHFP57_01960 HFP57_01965
AZLAZL_a09200 AZL_a09210
ALIAZOLI_p20648 AZOLI_p20649
TMOTMO_a0553 TMO_a0554(bphA)
FERFNB15_08990 FNB15_08995
NFRERS450000_00310(andAd)
AGRAAGRA3207_001975
ACTIUA75_12800
ACADUA74_12720
AHGAHOG_11995(nagG)
HRSHER32_17540
CGLENCTC11432_01739
 » show all
Reference
1  [PMID:9573207]
  Authors
Fuenmayor SL, Wild M, Boyes AL, Williams PA
  Title
A gene cluster encoding steps in conversion of naphthalene to gentisate in Pseudomonas sp. strain U2.
  Journal
J Bacteriol 180:2522-30 (1998)
DOI:10.1128/JB.180.9.2522-2530.1998
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 1.14.13.172
IUBMB Enzyme Nomenclature: 1.14.13.172
ExPASy - ENZYME nomenclature database: 1.14.13.172
BRENDA, the Enzyme Database: 1.14.13.172
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