KEGG ENZYME: 1.14.13.33

ENZYME: 1.14.13.33

Entry

EC 1.14.13.33               Enzyme

Name

4-hydroxybenzoate 3-monooxygenase [NAD(P)H];
4-hydroxybenzoate 3-monooxygenase (reduced nicotinamide adenine dinucleotide (phosphate));
4-hydroxybenzoate-3-hydroxylase;
4-hydroxybenzoate 3-hydroxylase

Class

Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor

Sysname

4-hydroxybenzoate,NAD(P)H:oxygen oxidoreductase (3-hydroxylating)

Reaction(IUBMB)

4-hydroxybenzoate + NAD(P)H + H+ + O2 = 3,4-dihydroxybenzoate + NAD(P)+ + H2O [RN:R01296 R01298]

Reaction(KEGG)

R01296 R01298

Substrate

4-hydroxybenzoate [CPD:C00156];
NADH [CPD:C00004];
NADPH [CPD:C00005];
H+ [CPD:C00080];
O2 [CPD:C00007]

Product

3,4-dihydroxybenzoate [CPD:C00230];
NAD+ [CPD:C00003];
NADP+ [CPD:C00006];
H2O [CPD:C00001]

Comment

A flavoprotein (FAD). The enzyme from Corynebacterium cyclohexanicum is highly specific for 4-hydroxybenzoate, but uses NADH and NADPH at approximately equal rates (cf. EC 1.14.13.2 4-hydroxybenzoate 3-monooxygenase). It is less specific for NADPH than EC 1.14.13.2.

History

EC 1.14.13.33 created 1989, modified 1999

Pathway

ec00362

Benzoate degradation

ec01100

Metabolic pathways

ec01120

Microbial metabolism in diverse environments

Reference

1 [PMID:3971979]

Authors

Fujii T, Kaneda T.

Title

Purification and properties of NADH/NADPH-dependent p-hydroxybenzoate hydroxylase from Corynebacterium cyclohexanicum.

Journal

Eur J Biochem 147:97-104 (1985)
DOI:10.1111/j.1432-1033.1985.tb08724.x

Reference

2 [PMID:8706756]

Authors

Seibold B, Matthes M, Eppink MH, Lingens F, Van Berkel WJ, Muller R

Title

4-Hydroxybenzoate hydroxylase from Pseudomonas sp. CBS3. Purification, characterization, gene cloning, sequence analysis and assignment of structural features determining the coenzyme specificity.

Journal

Eur J Biochem 239:469-78 (1996)
DOI:10.1111/j.1432-1033.1996.0469u.x

Other DBs

ExplorEnz - The Enzyme Database:

1.14.13.33

IUBMB Enzyme Nomenclature:

1.14.13.33

ExPASy - ENZYME nomenclature database:

1.14.13.33

BRENDA, the Enzyme Database:

1.14.13.33

CAS:	95471-33-3

LinkDB

DBGET integrated database retrieval system