KEGG ENZYME: 1.14.15.18

ENZYME: 1.14.15.18

Entry

EC 1.14.15.18               Enzyme

Name

calcidiol 1-monooxygenase;
25-hydroxycholecalciferol 1-hydroxylase;
25-hydroxycholecalciferol 1-monooxygenase;
1-hydroxylase-25-hydroxyvitamin D3;
25-hydroxy D3-1alpha-hydroxylase;
25-hydroxycholecalciferol 1alpha-hydroxylase;
25-hydroxyvitamin D3 1alpha-hydroxylase

Class

Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With reduced iron-sulfur protein as one donor, and incorporation of one atom of oxygen into the other donor

Sysname

calcidiol,adrenodoxin:oxygen oxidoreductase (1-hydroxylating)

Reaction(IUBMB)

(1) calcidiol + 2 reduced adrenodoxin + 2 H+ + O2 = calcitriol + 2 oxidized adrenodoxin + H2O [RN:R03610];
(2) secalciferol + 2 reduced adrenodoxin + 2 H+ + O2 = calcitetrol + 2 oxidized adrenodoxin + H2O [RN:R11324]

Reaction(KEGG)

R03610 R11324

Substrate

calcidiol [CPD:C01561];
reduced adrenodoxin [CPD:C00662];
H+ [CPD:C00080];
O2 [CPD:C00007];
secalciferol [CPD:C07712]

Product

calcitriol [CPD:C01673];
oxidized adrenodoxin [CPD:C00667];
H2O [CPD:C00001];
calcitetrol [CPD:C18231]

Comment

A P-450 (heme-thiolate) enzyme found in mammals.

History

EC 1.14.15.18 created 1976 as EC 1.14.13.13, transferred 2016 to EC 1.14.15.18

Pathway

ec00100

Steroid biosynthesis

ec01100

Metabolic pathways

Orthology

K07438

calcidiol 1-monooxygenase (25-hydroxyvitamin D3 1alpha-hydroxylase)

Genes

HSA:	1594(CYP27B1)

PTR:	452027(CYP27B1)

PPS:	100974264(CYP27B1)

GGO:

101146696

PON:

100443915

PPYG:

129009550

NLE:

100594622

HMH:

116480846

SSYN:

129465890

MCC:	715747(CYP27B1)

MCF:	102117707(CYP27B1)

MTHB:

126930378

MNI:

105474079

CSAB:

103238591(CYP27B1)

CATY:

105587234

PANU:

101009903

TGE:

112634833

MLEU:

105545594

RRO:

104682342

RBB:

108524407

TFN:

117089857

PTEH:

111541786

CANG:

105500481

CJC:	100388588(CYP27B1)

SBQ:

101042462

CIMI:

108309743(CYP27B1)

ANAN:

105729111(CYP27B1)

CSYR:

103264442

MMUR:

105877998

LCAT:

123639587

PCOQ:

105816771

OGA:

100961635

MMU:	13115(Cyp27b1)

MCAL:

110303958

MPAH:

110327080

RNO:	114700(Cyp27b1)

MCOC:

116075946

ANU:

117696705

MUN:

110561784

CGE:

100760414

MAUA:

101840476

PROB:

127215682

PLEU:

114704779

MORG:

121464015

MFOT:

126514764

AAMP:

119803686

NGI:

103742896

HGL:

101700851

CPOC:

100717678

CCAN:

109686086

DORD:

105990140

DSP:

122124179

PLOP:

125362044

NCAR:

124983979

MMMA:

107140112

OCU:

100341053

OPI:

101530921

TUP:

102492875

GVR:	103588480 103609177

CFA:	481133(CYP27B1)

CLUD:

112677953

VVP:

112922328

VLG:

121491053

NPO:

129511339

AML:

100481484

UMR:

103656705

UAH:

113256358

UAR:

123787194

ELK:

111160181

LLV:

125106498

MPUF:

101674682

MNP:

132019859

MLK:

131838536

NVS:

122892188

ORO:

101363421

EJU:

114215050

ZCA:

113922553

MLX:

118008655

NSU:

110577862

LWW:

102746290

FCA:	100750221(CYP27B1)

PYU:

121040851

PCOO:

112863857

PBG:

122473840

PVIV:

125169980

LRUF:

124501071

PTG:

102971063

PPAD:

109270394

PUC:

125919261

AJU:

106975877

HHV:

120248015

BTA:	539630(CYP27B1)

BOM:

102278257

BIU:

109559305

BBUB:

102414554

BBIS:

104988136

CHX:

102170758

OAS:

101116039

BTAX:

128048747

ODA:

120857512

CCAD:

122427212

MBEZ:

129537200

SSC:	397055(CYP27B1)

CFR:

102522811

CBAI:

105062143

CDK:

105100328

VPC:

102532825

BACU:

103001013

BMUS:

118901854

LVE:

103084276

OOR:

101284262

DLE:

111173817

PCAD:

102984886

PSIU:

116760774

NASI:

112394343

ECB:	100034100(CYP27B1)

EPZ:

103553643

EAI:

106833020

MYB:

102262846

MYD:

102757775

MMYO:

118649575

MLF:

102431071

MDT:

132227832

PKL:

118730267

EFUS:

103292480

MNA:

107546167

DRO:

112319168

SHON:

118975885

AJM:

119065237

PDIC:

114513469

PHAS:

123812846

MMF:

118623548

PPAM:

129076031

HAI:

109390756

RFQ:

117028656

PALE:

102894468

PGIG:

120604970

PVP:

105307396

RAY:

107501490

MJV:

108387803

TOD:

119248104

SARA:

101536895

SETR:

126022227

LAV:

100657886

TMU:

101355020

ETF:

101646226

DNM:

101414278

MDO:

100031497

GAS:

123250198

SHR:

100913970

AFZ:

127538226

PCW:

110210991

TVP:

118852217

OAA:

114814717

LSR:

110473893

SCAN:

103824978

PMOA:

120499536

OTC:

121334301

PRUF:

121356351

FAB:

101816678

OMA:

130265843

PHI:

102103763

PMAJ:

107199279

CBRC:

103611929

ZLE:

135459043

ACHL:

103807649

SVG:

106862648

HRT:

120748275

SATI:

136372739(CYP27B1)

SHAB:

115618603

TALA:

116963700 122153819

ACHC:

115350948

HLE:

104832391

AGEN:

126037398

DPUB:

104304660

NNI:

104021543

CPEA:

104393340

AROW:

112972148

NPD:

112952263

ASN:

102386966

AMJ:

102574984

CPOO:

109324487

PSS:

102443605

CMY:

102946801

CCAY:

125627714

DCC:

119845937

CPIC:

101954100

TST:

117888845

CABI:

116828443

MRV:

120391239

ACS:

100559713

ASAO:

132766522

PVT:

110074451

SUND:

121920838

PBI:

103060795

PMUR:

107291644

CTIG:

120297684

TSR:

106552999

PGUT:

117662682

APRI:

131191545

PTEX:

113445553

NSS:

113418481

VKO:

123029856

PMUA:

114593032

PRAF:

128405812

ZVI:

118078986

HCG:

128343754

GJA:

107119675

STOW:

125427926

EMC:

129346642

XLA:	108708601(cyp27b1.L) 108709871(cyp27b1.S)

XTR:	448754(cyp27b1)

NPR:

108791588

RTEM:

120929439

BBUF:

120996179

BGAR:

122931986

MUO:

115465067

GSH:

117357711

DRE:	793309(cyp27b1)

SRX:

107747831

SANH:

107684010

SGH:

107561793

CCAR:

109089182

CAUA:

113068164

CGIB:

127968595

PTET:

122353667

LROH:

127173226

OMC:

131549861

PPRM:

120467743

RKG:

130077768

MAMB:

125256888 125261911

CIDE:

127522570

CERY:

137021300(cyp27b1)

MASI:

127428324

TROS:

130551291

TDW:

130410704

MANU:

129427074

IPU:

108254794

IFU:

128625019

SMEO:

124402328

TFD:

113639045

TVC:

132838018

TRN:

134301741

AMEX:

103035224

CMAO:

118799357

EEE:

113572450

CHAR:

105907733

TRU:

101062641

TFS:

130524606

TNG:	GSTEN00033821G001

LCO:

109142364

NCC:

104955571

TBEN:

117489730

CGOB:

115008521

PGEO:

117446167

GACU:

117534149

EMAC:

134882379

ELY:

117257290

EFO:

125895893

PLEP:

121956007

SLUC:

116066246

ECRA:

117942881

ESP:	116679836 116687227

PFLV:

114554512

GAT:

120835315

PPUG:

119194159

AFB:

129105820

CLUM:

117731316

PSWI:

130208381

MSAM:

119886596

SCHU:

122864359

CUD:

121528681

ALAT:

119021281

MZE:

101476393

ONL:

100706100

OAU:

116326616

OLA:

101172325

OML:

112145670

CSAI:

133448712

XMA:

102222483

XCO:

114134887

XHE:

116709734

PRET:

103465325

PFOR:

103129584

PLAI:

106935833

PMEI:

106929457

GAF:

122834207

PPRL:

129358301

CVG:

107082096

CTUL:

119795482

GMU:

124856373

NFU:

107385653

KMR:

108235620

ALIM:

106518084

NWH:

119412594

AOCE:

111587639

MCEP:

125007137

CSEM:

103386049

POV:

109628943

SSEN:

122767755

HHIP:

117761420

HSP:

118104984

PPLT:

128449127

SMAU:

118310005

LCF:

108898968

SDU:

111221905

SLAL:

111667688

XGL:

120806983

HCQ:

109522134

SSCV:

125976793

SBIA:

133507929

PEE:

133408676

PTAO:

133484068

BPEC:

110171326

MALB:

109956137

BSPL:

114856290

SJO:

128356147

SASA:

106565446 106583283

STRU:

115150468 115207954

OTW:	112254699 112262073

OMY:	110494198 110528221

OGO:	124003988 124019410

ONE:	115143688 115146075

OKI:	109882615 109902824

OKE:	118389508 118400368

SALP:

111965588 111970194

SNH:	120062013 120064579

CCLU:

121575162 121577839

ELS:

105017033

SFM:	108918367 108939170

PKI:

111854850

AANG:

118210837

LOC:

102685924

PSPA:

121308962

ARUT:

117400985 117966943

PSEX:

120524845

LCM:

102346327

CMK:

103191415

RTP:

109925523

CPLA:

122543275

HOC:

132805718

LERI:

129714633

PMRN:

116941144

LRJ:

133344635

ARUN:

117289714

PPOT:

106099942

CQD:

128705324

PVUL:

126815583 126817612 126819097

BGT:

106053581

GAE:

121389521

CRG:

105346613

CANU:

128189445

MMER:

123550682

RPHI:

132726491

DPOL:

127856113

MAEA:

128243703

OSN:

115209661

LJP:	135465801 135466673

ATEN:

116299173 116299174

» show all

Reference

1 [PMID:4404596]

Authors

Gray RW, Omdahl JL, Ghazarian JG, DeLuca HF.

Title

25-Hydroxycholecalciferol-1-hydroxylase. Subcellular location and properties.

Journal

J Biol Chem 247:7528-32 (1972)

Reference

2 [PMID:10092858]

Authors

Sakaki T, Sawada N, Takeyama K, Kato S, Inouye K

Title

Enzymatic properties of mouse 25-hydroxyvitamin D3 1 alpha-hydroxylase expressed in Escherichia coli.

Journal

Eur J Biochem 259:731-8 (1999)
DOI:10.1046/j.1432-1327.1999.00096.x

Reference

3 [PMID:10518789]

Authors

Sawada N, Sakaki T, Kitanaka S, Takeyama K, Kato S, Inouye K

Title

Enzymatic properties of human 25-hydroxyvitamin D3 1alpha-hydroxylase coexpression with adrenodoxin and NADPH-adrenodoxin reductase in Escherichia coli.

Journal

Eur J Biochem 265:950-6 (1999)
DOI:10.1046/j.1432-1327.1999.00794.x

Other DBs

ExplorEnz - The Enzyme Database:

1.14.15.18

IUBMB Enzyme Nomenclature:

1.14.15.18

ExPASy - ENZYME nomenclature database:

1.14.15.18

BRENDA, the Enzyme Database:

1.14.15.18

CAS:

9081-36-1

LinkDB

DBGET integrated database retrieval system