KEGG   ENZYME: 1.14.19.21
Entry
EC 1.14.19.21               Enzyme                                 
Name
cholesterol 7-desaturase;
nvd (gene name);
daf-36 (gene name)
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With oxidation of a pair of donors resulting in the reduction of O2 to two molecules of water
Sysname
cholesterol,NAD(P)H:oxygen oxidoreductase (7,8 dehydrogenating)
Reaction(IUBMB)
cholesterol + O2 + NAD(P)H + H+ = cholesta-5,7-dien-3beta-ol + NAD(P)+ + 2 H2O [RN:R11007 R11008]
Reaction(KEGG)
R11007 R11008
Substrate
cholesterol [CPD:C00187];
O2 [CPD:C00007];
NADH [CPD:C00004];
NADPH [CPD:C00005];
H+ [CPD:C00080]
Product
cholesta-5,7-dien-3beta-ol [CPD:C01164];
NAD+ [CPD:C00003];
NADP+ [CPD:C00006];
H2O [CPD:C00001]
Comment
The enzyme, characterized from several organisms including the worm Caenorhabditis elegans, the fly Drosophila melanogaster, and the ciliate Tetrahymena thermophila, is a Rieske oxygenase. In insects it participates in the the biosythesis of ecdysteroid hormones. The electrons are transferred from NAD(P)H via an electron transfer chain likely to include ferredoxin reductase and ferredoxin. The enzyme differs from regular desaturases, such as EC 1.14.19.20, 7-sterol 5(6)-desaturase, which are cytochrome b5-dependent and contain the three His-boxes that are typical to most desaturases.
History
EC 1.14.19.21 created 2015
Pathway
ec00981  Insect hormone biosynthesis
Orthology
K14938  cholesterol 7-desaturase
Genes
GGA427775
PCOC116234422
MGP104913710
CJO107321655
LMUT125702752
NMEL110407053
APLA101805447
ACYG125180915
CATA118257067
AFUL116497035
ETL114064164
FPG101919122
FCH102047190
CLV102098254
EGZ104124000
NNI104022360
PLET104627819
EHS104505667
PCAO104048586
ACUN113486626
TALA104361340
PADL103918965
AFOR103902418
ACHC115335319
HALD104310400
HLE104830020
AGEN126052516
GCL127023818
CCRI104154540
CSTI104549732
CMAC104480871
BREG104629094
FGA104078860
GSTE104264726
OHA104335383
NNT104402505
SHAB115617191
PGUU104465442
AVIT104267132
CVF104291118
RTD128917689
CUCA104059142
TEO104381409
AAM106487012
AROW112963078
NPD112955952
TGT104573347
DNE112991357
SCAM104139811
ASN102371146
AMJ102565749
CPOO109314108
GGN109292718
PSS102455749
CMY102936991
CCAY125623666
DCC119844323
CPIC101940467
TST117867185
CABI116820424
MRV120386933
ACS100558783
ASAO132763612
PVT110087450
SUND121936429
PBI103066999
PMUR107302524
CTIG120308615
TSR106548819
PGUT117676660
APRI131185942
PTEX113444025
NSS113424726
VKO123028208
PMUA114590059 114592319 114592320
PRAF128409391
ZVI118080215
HCG128339121
GJA107120404
STOW125442159
EMC129342293
XLA108698103(XB5992703.L) 779348(XB5992703.S)
XTR100216141
NPR108795927
RTEM120913993
BBUF120977408 120977409
BGAR122919655 122946149 122946150 122946762
MUO115472163
GSH117367721
DRE436885(zgc:92275)
SRX107739715 107754619
SANH107662064 107665231
SGH107584623
CCAR109045525
CAUA113063929
CGIB127941321 127985608(zgc:92275)
PTET122326366(zgc:92275)
LROH127153055(zgc:92275)
OMC131528618(zgc:92275)
PPRM120490655(zgc:92275)
RKG130090783(zgc:92275)
MAMB125252778(zgc:92275)
CIDE127503337 127525455
MASI127428623(zgc:92275) 127431275
TROS130570563(zgc:92275)
TDW130407467(zgc:92275)
MANU129424406(zgc:92275)
IPU108279335(zgc:92275)
IFU128622753(zgc:92275)
PHYP113535713
SMEO124397555(zgc:92275)
TFD113646467(zgc:92275)
TVC132860435(zgc:92275)
TRN134330418(zgc:92275)
AMEX103022783(zgc:92275)
CMAO118820347(zgc:92275)
EEE113573769
CHAR105902521(zgc:92275)
TRU101069484
TFS130517529(zgc:92275)
TNGGSTEN00005122G001
LCO104936331
NCC104958128
TBEN117478759(zgc:92275)
CGOB115016994
PGEO117456530(zgc:92275)
GACU117539975(zgc:92275)
EMAC134868721(zgc:92275)
ELY117269523(zgc:92275)
EFO125906002(zgc:92275)
PLEP121960166(zgc:92275)
SLUC116034845(zgc:92275)
ECRA117959585(zgc:92275)
ESP116704543
PFLV114571346
GAT120831891(zgc:92275)
PPUG119226249(zgc:92275)
AFB129102857(zgc:92275)
CLUM117740739(zgc:92275)
PSWI130205659(zgc:92275)
MSAM119914737(zgc:92275)
SCHU122865580(zgc:92275)
CUD121524811(zgc:92275)
ALAT119030527(zgc:92275)
MZE101475379
ONL100702604
OAU116319597(zgc:92275)
OLA101170113 105358384
OML112162855(zgc:92275)
CSAI133454753(zgc:92275)
XMA102227123
XCO114150182
XHE116724557
PRET103473999
PFOR103154387
PLAI106937655
PMEI106907543
GAF122819185(zgc:92275)
PPRL129366705(zgc:92275)
CVG107102253
CTUL119772015(zgc:92275)
GMU124873052(zgc:92275)
NFU107375133
KMR108241378(zgc:92275)
ALIM106513553 106533739
NWH119424478(zgc:92275)
AOCE111571560
MCEP124996303(zgc:92275)
CSEM103389306
POV109640312
SSEN122758614(zgc:92275)
HHIP117771622(zgc:92275)
HSP118125834(zgc:92275)
PPLT128425254(zgc:92275)
SMAU118284944(zgc:92275)
LCF108894422(zgc:92275)
SDU111238492
SLAL111650147
XGL120806669(zgc:92275)
SSCV125979739(zgc:92275)
SBIA133491127(zgc:92275)
PEE133414054(zgc:92275)
PTAO133490188(zgc:92275)
BPEC110172811
BSPL114866897(zgc:92275)
SJO128380674(zgc:92275)
SASA106565467
STRU115159509
OTW112219356(zgc:92275)
OMY110523638(zgc:92275)
OGO124048900(zgc:92275)
ONE115123474
OKI109868855 109873612
OKE118387577(zgc:92275)
SALP111961562
SNH120025543(zgc:92275)
CCLU121582460
ELS105024189
SFM108922591
PKI111853969
AANG118206903(zgc:92275)
LOC102691906
PSPA121306732(zgc:92275)
ARUT117396731(zgc:92275) 117974032
PSEX120542954(zgc:92275)
LCM102356630
CMK103183010(zgc:92275)
CPLA122564641(zgc:92275)
HOC132826018(zgc:92275)
LERI129712167(zgc:92275)
PMRN116957437
LRJ133361177
BFO118414807 118420780
BBEL109466201 109482902
CIN100176071 100177386
SCLV120327085 120337189 120337310 120337807 120343680
SPU592788
LPIC129277485 129277488
APLC110977513 110977515 110977953 110977954 110980559 110985648 110986001
AJC117100009
SKO100374996
DMEDmel_CG40050(nvd)
DER26527012
DYADyak_GE28326
DSR110187751
DPO26533291
DPE113565812
DMN108153908
DWI6648040
DGR116806032
DMODmoj_GI25582
DAZ108621623
DNV108649185
DHE111598510
CCAT101462794
BDR109579336
AOQ129236376
TDA119677549
SCAC106084232
LSQ119600883
GFS119635129
ECOE129942652
CLON129605773
HIS119656808
AGA1270530
ACOZ120957273
AARA120906649
AMER121596536
ASTE118502971
AFUN125771269
AMOU128306845
AALI118459676
AAG5574523
AALB109405340 115255620
ASUA134217994
CQUCpipJ_CPIJ006320
BCOO119069940 119075266
AME107965828
ACER108001271
ALAB122720233
ADR102679678
AFLR100864931
BIM100745011
BBIF117204682
BVK117232584
BVAN117156288
BTER100649007
BAFF126922147
BPYO122567532
BPAS132913080
FVI122532044
CCAL108631726
OBB114876484
OLG117603160
MGEN117224230
NMEA116426513
CGIG122399135
SOC105204704
MPHA105836516
AEC105154519
ACEP105621110 105621113
PBAR105430807
VEM105563098
HST105187219
DQU106741859
CFO105251516
FEX115236188
LHU105674672
PGC109856152
OBO105277313
PCF106783952
PFUC122524986
VPS122627660
VCRB124422779
VVE124947659
NVI100119735 100119998
CSOL105367830
TPRE106651441
LHT122498793
LBD127279370
MDL103572543 103573967
CGLO123262462
FAS105262869
DAM107037320
AGIF122855124
CINS118073524
VCAN122406404
CCIN107270451
DSM124413684
NPT124222514
NFB124184555
NLO107225306
NVG124307222
AROA105688348
BMOR733067(Nvd)
BMAN114251510
MSEX115451343
DPLKGM_215135
BANY112048798
MJU123879537
NIQ126779861
VCD124542283
MCIX123666034
PMAC106708229 106708230
PPOT106105902
PXU106126060
PRAP110997305
PBX123715475
PNAP125061310
ZCE119838466 119838565
CCRC123704539 123704683 123704684
LSIN126973934
AAGE121736562
HAW110382619
HZE124644511
TNL113509023
SLIU111360496
OFU114360096 114362450
ATRA106139478
GMW113521084
PXY105380845
PGW126379673
LGLY125239468
CFEL113367651 113367652
CCRN123296378
API100162250
DNX107165578
AGS114126496
RMD113551662
ACOO126847735
DVT126895184
BTAB109029997 109030017 109030061 109030063 109030501 109030523 109032522 109036892 109043093
DCI103506558 103506559
CLEC106673050
HHAL106685329
NLU111056285
HVI124356117
MQU128987015
PHUPhum_PHUM458690
FOC113204432
TPAL117641049
ZNE110826966
CSEC111867050
SGRE126365945
BROR134535909
IEL124155461
FCD110842436 110846580 110846657 110848904 110854554 110854966 110857831 110861595
DPXDAPPUDRAFT_18823 DAPPUDRAFT_308492 DAPPUDRAFT_311567
DMK116926277 116932277
DPZ124315332 124335932
PVM113823163
PJA122253458
PCHN125026412
PMOO119580273
HAME121872029
PCLA123757872
CQD128698025
PTRU123515039
ESN126982508
MNZ135206105
HAZT108673947
EAF111708930 111716479
LSM121127772
TCF131888659
PPOI119111722
ISC8034515
DSV119437085 119437095 119437107 119437150 119451449 119451477 119451504
RSAN119379265 119386907 119398458 119398507
RMP119161394 119161395 119162163 119162376
TUT107371675
DPTE113795498
DFR124489826
CSCU111614253 111614257 111614720 111615414 111617134 111621076 111621087 111623385 111629464 111629490 111630269 111630271 111633589 111633591 111633595 111636474 111637897 111638991 111639843 111640992 111641705 111641850
PTEP107441755
ABRU129957125
UDV129221062 129224184
SDM118201532
LPOL106459473
CELCELE_C12D8.5(daf-36)
CBRCBG_09553(Cbr-daf-36)
CRQGCK72_017584 GCK72_025847
NAINECAME_15984
BMYBM_BM11695(Bma-daf-36)
LOALOAG_18474
HROHELRODRAFT_107375 HELRODRAFT_87564
LGILOTGIDRAFT_130105
PVUL126814563
PCAN112575618
BGT106064009
GAE121382624 121384285
HRF124138371 124138372
HRJ124267628 124267629
CRG105340983 105340985
CVN111123313 111123314 111126872 111127059
CANU128155544 128156847
OED125681243 125681336
MYI110464414
PMAX117321955
MCAF127728652
MMER123557356 123557370
RPHI132754004 132754005 132754007
DPOL127837758 127856245
MAEA128223078
OBI106883186
OSN115223821
LJP135480985
LAK106164789 106177872
LLON135501368
NVE5515211
EPA110251413
ATEN116304131
ADF107344862
AMIL114947222
PDAM113681662
SPIS111326686
DGT114530380
XEN124439673
AQU100634894
NCOL116268054
SREPTSG_07043
ACANACA1_048400 ACA1_164170
PTMGSPATT00025959001 GSPATT00032008001 GSPATT00035183001 GSPATT00035190001 GSPATT00036085001
SMINv1.2.004636.t1(symbB.v1.2.004636.t1)
AAFAURANDRAFT_31508
NGRNAEGRDRAFT_78012 NAEGRDRAFT_78121
FAUFraau_1241
HALCEY643_18085
SOKD0B54_08475
BMJBMULJ_05577
BMUBmul_5949
BMKDM80_6398
VBOCKY39_30055
SRCM271_41250
SMALSMALA_1177
SAUOBV401_09655
SGVB1H19_02775
TCUTcur_2516
PSEAWY02_22000
PSEHXF36_05215
AYMYM304_00970
TERTery_0576
 » show all
Reference
1  [PMID:21632547]
  Authors
Yoshiyama-Yanagawa T, Enya S, Shimada-Niwa Y, Yaguchi S, Haramoto Y, Matsuya T, Shiomi K, Sasakura Y, Takahashi S, Asashima M, Kataoka H, Niwa R
  Title
The conserved Rieske oxygenase DAF-36/Neverland is a novel cholesterol-metabolizing enzyme.
  Journal
J Biol Chem 286:25756-62 (2011)
DOI:10.1074/jbc.M111.244384
  Sequence
Reference
2  [PMID:21749634]
  Authors
Wollam J, Magomedova L, Magner DB, Shen Y, Rottiers V, Motola DL, Mangelsdorf DJ, Cummins CL, Antebi A
  Title
The Rieske oxygenase DAF-36 functions as a cholesterol 7-desaturase in steroidogenic pathways governing longevity.
  Journal
Aging Cell 10:879-84 (2011)
DOI:10.1111/j.1474-9726.2011.00733.x
  Sequence
Reference
3  [PMID:23603937]
  Authors
Najle SR, Nusblat AD, Nudel CB, Uttaro AD
  Title
The Sterol-C7 desaturase from the ciliate Tetrahymena thermophila is a Rieske Oxygenase, which is highly conserved in animals.
  Journal
Mol Biol Evol 30:1630-43 (2013)
DOI:10.1093/molbev/mst076
Reference
4  [PMID:24244885]
  Authors
Barry SM, Challis GL
  Title
Mechanism and Catalytic Diversity of Rieske Non-Heme Iron-Dependent Oxygenases.
  Journal
ACS Catal 3:2362–2370 (2013)
DOI:10.1021/cs400087p
Other DBs
ExplorEnz - The Enzyme Database: 1.14.19.21
IUBMB Enzyme Nomenclature: 1.14.19.21
ExPASy - ENZYME nomenclature database: 1.14.19.21
BRENDA, the Enzyme Database: 1.14.19.21
LinkDB

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