KEGG   ENZYME: 1.4.1.12
Entry
EC 1.4.1.12                 Enzyme                                 

Name
2,4-diaminopentanoate dehydrogenase;
2,4-diaminopentanoic acid C4 dehydrogenase
Class
Oxidoreductases;
Acting on the CH-NH2 group of donors;
With NAD+ or NADP+ as acceptor
Sysname
(2R,4S)-2,4-diaminopentanoate:NAD(P)+ oxidoreductase (deaminating)
Reaction(IUBMB)
(2R,4S)-2,4-diaminopentanoate + H2O + NAD(P)+ = (2R)-2-amino-4-oxopentanoate + NH3 + NAD(P)H + H+ [RN:R04200 R04201]
Reaction(KEGG)
Substrate
(2R,4S)-2,4-diaminopentanoate [CPD:C03943];
H2O [CPD:C00001];
NAD+ [CPD:C00003];
NADP+ [CPD:C00006]
Product
(2R)-2-amino-4-oxopentanoate [CPD:C03341];
NH3 [CPD:C00014];
NADH [CPD:C00004];
NADPH [CPD:C00005];
H+ [CPD:C00080]
Comment
Also acts, more slowly, on 2,5-diaminohexanoate forming 2-amino-5-oxohexanoate, which then cyclizes non-enzymically to 1-pyrroline-2-methyl-5-carboxylate. It has equal activity with NAD+ and NADP+ [cf. EC 1.4.1.26, 2,4-diaminopentanoate dehydrogenase (NAD+)].
History
EC 1.4.1.12 created 1976, modified 2017
Pathway
ec00310  Lysine degradation
ec00330  Arginine and proline metabolism
ec00470  D-Amino acid metabolism
ec01100  Metabolic pathways
Orthology
K21672  2,4-diaminopentanoate dehydrogenase
Genes
CEH: CEW89_15600
EAV: EH197_05050
VCP: H9L18_06400
VHY: G7082_14535
CSQ: CSCA_0127
CACE: CACET_c17480(dapB2)
CEU: A7L45_16560
CFM: BJL90_14515
CARG: RSJ17_08675
CDRK: B9W14_17905
AMT: Amet_2367
AOE: Clos_1699
CDF: CD630_04420(ord)
PDC: CDIF630_00570(ord)
PDF: CD630DERM_04420(ord)
CST: CLOST_1294(ord)
TTE: TTE1234
THX: Thet_1426
TOC: Toce_1489
KME: H0A61_00511(ord)
PFT: JBW_01651
MPHU: MPHO_01310
PAC: PPA2276
PAW: PAZ_c23740(dapB2)
PACC: PAC1_11610
PACH: PAGK_2185
CACN: RN83_00010
SBR: SY1_03600
TME: Tmel_0905
TAF: THA_1160
THER: Y592_05345
FNO: Fnod_1646
OCY: OSSY52_03900(ord)
PMO: Pmob_1166
MARN: LN42_08170
DTN: DTL3_0759
KOL: Kole_0356
ASAC: ATHSA_0997
 » show all
Reference
1  [PMID:4684685]
  Authors
Somack R, Costilow RN
  Title
2,4-diaminopentanoic acid C 4 dehydrogenase. Purification and properties of the protein.
  Journal
J Biol Chem 248:385-8 (1973)
  Sequence
Reference
2
  Authors
Stadtman, T.C.
  Title
Lysine metabolism by clostridia. XIIB 2,4-Diaminohexanoate dehydrogenase (2,4-diaminopentanoate dehydrogenase).
  Journal
Adv Enzymol Relat Areas Mol Biol 38:441-445 (1973)
Reference
3  [PMID:4394942]
  Authors
Tsuda Y, Friedmann HC.
  Title
Ornithine metabolism by Clostridium sticklandii. Oxidation of ornithine to 2-amino-4-ketopentanoic acid via 2,4-diaminopentanoic acid; participation of B12 coenzyme, pyridoxal phosphate, and pyridine nucleotide.
  Journal
J Biol Chem 245:5914-26 (1970)
Other DBs
ExplorEnz - The Enzyme Database: 1.4.1.12
IUBMB Enzyme Nomenclature: 1.4.1.12
ExPASy - ENZYME nomenclature database: 1.4.1.12
BRENDA, the Enzyme Database: 1.4.1.12
CAS: 39346-26-4
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