Oxidoreductases;
Acting on the CH-NH2 group of donors;
With NAD+ or NADP+ as acceptor
BRITE hierarchy

L-glutamate:NAD+ oxidoreductase (deaminating)

L-glutamate + H2O + NAD+ = 2-oxoglutarate + NH3 + NADH + H+ (overall reaction) [RN:R00243];
(1a) L-glutamate + NAD+ = 2-iminoglutarate + NADH + H+ [RN:R13714];
(1b) 2-iminoglutarate + H2O = 2-oxoglutarate + NH3 [RN:R13715]

R00243 R13714 R13715;
(other) R00145 R00146
Reaction

L-glutamate [CPD:C00025];
H2O [CPD:C00001];
NAD+ [CPD:C00003];
2-iminoglutarate [CPD:C23180]

The enzyme is generally involved in the degradation of glutamate into 2-oxoglutarate and ammonia and plays an important role in the interconversion of nitrogen and carbon metabolism. The reaction starts by oxidation of glutamate to 2-iminoglutarate by NAD+, followed by hydrolysis of the latter to 2-oxoglutarate and ammonia. A related NADP-dependent enzyme (EC 1.4.1.4, glutamate dehydrogenase (NADP+)) catalyses the reaction in the opposite direction and plays a role in ammonia anabolism. Some enzymes show dual co-substrate specificity (EC 1.4.1.3, glutamate dehydrogenase [NAD(P)+]).

EC 1.4.1.2 created 1961