KEGG ENZYME: 1.5.1.52

ENZYME: 1.5.1.52

Entry

EC 1.5.1.52                 Enzyme

Name

staphylopine dehydrogenase;
cntM (gene name);
staphylopine synthase

Class

Oxidoreductases;
Acting on the CH-NH group of donors;
With NAD+ or NADP+ as acceptor

Sysname

staphylopine:NADP+ oxidoreductase [(2S)-2-amino-4-{[(1R)-1-carboxy-2-(1H-imidazol-4-yl)ethyl]amino}butanoate]-forming

Reaction(IUBMB)

staphylopine + NADP+ + H2O = (2S)-2-amino-4-{[(1R)-1-carboxy-2-(1H-imidazol-4-yl)ethyl]amino}butanoate + pyruvate + NADPH + H+ [RN:R12237]

Reaction(KEGG)

R12237

Substrate

staphylopine [CPD:C22024];
NADP+ [CPD:C00006];
H2O [CPD:C00001]

Product

(2S)-2-amino-4-{[(1R)-1-carboxy-2-(1H-imidazol-4-yl)ethyl]amino}butanoate [CPD:C22025];
pyruvate [CPD:C00022];
NADPH [CPD:C00005];
H+ [CPD:C00080]

Comment

The enzyme, characterized from the bacterium Staphylococcus aureus, catalyses the last reaction in the biosynthesis of the metallophore staphylopine, which is involved in the acquisition of nickel, copper, and cobalt.

History

EC 1.5.1.52 created 2018

Pathway

ec00470

D-Amino acid metabolism

ec01100

Metabolic pathways

Orthology

K23122

staphylopine/pseudopaline/yersinopine synthase

Genes

YPE:

YPO1347

YPK:

y2835

YPH:

YPC_2840

YPA:

YPA_0633

YPN:

YPN_2637

YPM:

YP_1249

YPP:	YPDSF_2352

YPG:	YpAngola_A1577

YPZ:

YPZ3_1229

YPT:	A1122_18850

YPD:

YPD4_1192

YPX:

YPD8_0953

YPW:

CH59_495

YPJ:

CH55_1201

YPV:

BZ15_2207

YPL:

CH46_3782

YPS:

YPTB1373

YPO:

BZ17_1146

YPI:	YpsIP31758_2631

YPY:

YPK_2716

YPB:

YPTS_1472

YPQ:

DJ40_855

YPU:

BZ21_649

YPR:

BZ20_606

YPC:

BZ23_983

YPF:

BZ19_781

YSI:	BF17_15915

SMW:	SMWW4_v1c21330

SERF:

L085_18090

SERS:

SERRSCBI_09990

SSUR:

ATE40_007150

SURI:

J0X03_13350

SNEM:

NLX84_10750

SNEV:

OI978_22605

SERB:

QEP77_11955

SERH:

GF111_01775

ANS:	ArsFIN_50680

VNI:	VIBNI_B0489

VAQ:	FIV01_17875

PAE:

PA4835

PAEV:

N297_5004

PAEI:

N296_5004

PAU:	PA14_63920

PAG:	PLES_52201

PAF:	PAM18_4946

PNC:	NCGM2_0694

PAEB:

NCGM1900_5573

PDK:	PADK2_25660

PSG:	G655_25505

PRP:	M062_25520

PAEP:

PA1S_26280

PAER:

PA1R_gp2756

PAEM:

U769_26535

PAEL:

T223_26730

PAES:

SCV20265_5493

PAEU:

BN889_05384

PAEG:

AI22_07720

PAEC:

M802_5002

PAEO:

M801_4869

SECH:

B18_11690

PAP:	PSPA7_5554

PPAA:

B7D75_25160

SDO:	SD1155_03775

BSON:

S101395_03011

BHA:

BH2073

LSP:

Bsph_1831

LFU:	HR49_11880

LYB:	C3943_14845

LYC:	FH508_0007345

LYO:	NV349_06630

MEKU:

HUW50_21730

MDG:	K8L98_19845

MSEE:

WCV65_15735

SLMS:

MM221_10315

FPN:	ABE65_005810

FAR:	ABE41_005935

AND:	GRQ40_16000

SA2256

SAV:

SAV2468

SAW:

SAHV_2452

SAH:	SaurJH1_2544

SAJ:	SaurJH9_2495

SAM:

MW2392

SAS:

SAS2359

SAR:

SAR2555

SAC:

SACOL2477

SAX:	USA300HOU_2459

SAA:	SAUSA300_2412

SAO:	SAOUHSC_02768

SAE:

NWMN_2365

SAD:

SAAV_2534

SUU:	M013TW_2428(cntM)

SUV:	SAVC_11210

SUE:	SAOV_2518c

SUJ:	SAA6159_02365(cntM)

SUK:	SAA6008_02509

SUC:	ECTR2_2325

SUT:	SAT0131_02667

SUQ:	HMPREF0772_10721

SUZ:

MS7_2481

SUD:	ST398NM01_2518

SUX:	SAEMRSA15_23660

SUW:	SATW20_25960

SUG:

SAPIG2518

SUF:	SARLGA251_22480

SAUA:

SAAG_00294

SAUE:

RSAU_002306

SAUN:

SAKOR_02452

SAUS:

SA40_2216

SAUU:

SA957_2300

SAUG:

SA268_2373

SAUZ:

SAZ172_2571

SAUT:

SAI1T1_2018380

SAUJ:

SAI2T2_1018390

SAUK:

SAI3T3_1018380

SAUQ:

SAI4T8_1018390

SAUV:

SAI7S6_1018380

SAUW:

SAI5S5_1018320

SAUX:

SAI6T6_1018320

SAUY:

SAI8T7_1018360

SAUF:

X998_2452

SAB:

SAB2351c

SUY:	SA2981_2405

SAUB:

C248_2518

SAUM:

BN843_25080

SAUC:

CA347_2547

SAUR:

SABB_01209

SAUI:

AZ30_12960

SAUD:

CH52_06645

SAMS:

NI36_12270

SUH:	SAMSHR1132_22930

SER:

SERP2373

SEP:

SE_0203

SEPP:

SEB_00131

SEPS:

DP17_1261

SSD:	SPSINT_0078

SDT:

SPSE_2399

SDP:	NCTC12225_02594

SWA:	A284_00420

SPAS:

STP1_1304

SXY:	BE24_11755

SXL:	SXYLSMQ121_0069

SXO:	SXYL_00070

SHU:	SHYC_11645

SSCH:

LH95_11150

SSCZ:

RN70_11900

SAGQ:

EP23_10520

SLZ:	B5P37_06435

SFQ:	C7J90_03775

SCAR:

DWB96_00450(cntM)

SCHR:

DWB92_11720

SSH:	NCTC13712_02420

SLLO:

ISP08_00450(cntM)

SRAI:

LN051_10975

SPSD:

JMB28_00745(cntM)

SEDA:

MNY58_00465

SROT:

ML435_11875(cntM)

SFF:	FOB90_08240(cntM)

SSTE:

SAMEA4384403_1090

BBE:	BBR47_28220

BFM:	BP422_09285

BAGR:

BA6348_01045

BPAB:

PSE45_01835

BREH:

HP435_01970

GYM:	GYMC10_2837

PMS:	KNP414_01895

PMQ:	PM3016_2180

PMW:

B2K_11205

PAEA:

R70723_16830

PNP:	IJ22_25010

PVO:	PVOR_17329

PLW:	D5F53_15600

PCHI:

PC41400_19300 PC41400_19390

PPRT:

ET464_05565 ET464_05570

PBAC:

HUB98_11420

PTRI:

KDC22_17095

PALO:

E6C60_2222

PUI:	PUW25_11740

PAMY:

P9222_12200

PNK:	AASFL403_06950

PPAB:

KET34_28285

PEZZ:

HW560_11455

PANA:

BBH88_13385

CFM:	BJL90_00600

CARG:

RSJ17_16755

HHW:	NCTC503_01796

GPD:	GII33_20950

ARTQ:

CGQ24_13610

AAI:	AARI_10900

GAR:	AOZ07_05775

GLU:	F0M17_05435

GNC:	QQS42_05450

PAEY:

KUF55_05520

PSDS:

K1T35_28800

APRE:

CNX65_19680

AMI:

Amir_3918

UME:	RM788_04610

PLK:	CIK06_26265

FNT:	HMPREF0405_01276

FNE:	FSDG_01139

FVA:	FV113G1_15600

FUL:	C4N20_14590

FMO:	C4N19_08465

» show all

Reference

1 [PMID:27230378]

Authors

Ghssein G, Brutesco C, Ouerdane L, Fojcik C, Izaute A, Wang S, Hajjar C, Lobinski R, Lemaire D, Richaud P, Voulhoux R, Espaillat A, Cava F, Pignol D, Borezee-Durant E, Arnoux P

Title

Biosynthesis of a broad-spectrum nicotianamine-like metallophore in Staphylococcus aureus.

Journal

Science 352:1105-9 (2016)
DOI:10.1126/science.aaf1018

Sequence

[sav:SAV2468]

Reference

2 [PMID:29618515]

Authors

McFarlane JS, Davis CL, Lamb AL

Title

Staphylopine, pseudopaline, and yersinopine dehydrogenases: A structural and kinetic analysis of a new functional class of opine dehydrogenase.