KEGG   ENZYME: 2.3.1.235
Entry
EC 2.3.1.235                Enzyme                                 
Name
tetracenomycin F2 synthase;
TCM PKS
Class
Transferases;
Acyltransferases;
Transferring groups other than aminoacyl groups
Sysname
malonyl-CoA:acetate malonyltransferase (tetracenomycin-F2-forming)
Reaction(IUBMB)
10 malonyl-CoA = tetracenomycin F2 + 10 CoA + 10 CO2 + 2 H2O [RN:R10960]
Reaction(KEGG)
R10960;
(other) R06643 R09269
Substrate
malonyl-CoA [CPD:C00083]
Product
tetracenomycin F2 [CPD:C12366];
CoA [CPD:C00010];
CO2 [CPD:C00011];
H2O [CPD:C00001]
Comment
A multi-domain polyketide synthase involved in the synthesis of tetracenomycin in the bacterium Streptomyces glaucescens. It involves a ketosynthase complex (TcmKL), an acyl carrier protein (TcmM), a malonyl CoA:ACP acyltransferase (MAT), and a cyclase (TcmN). A malonyl-CoA molecule is initially bound to the acyl carrier protein and decarboxylated to form an acetyl starter unit. Additional two-carbon units are added from nine more malonyl-CoA molecules.
History
EC 2.3.1.235 created 2014
Pathway
ec01056  Biosynthesis of type II polyketide backbone
ec01100  Metabolic pathways
ec01110  Biosynthesis of secondary metabolites
Orthology
K05551  minimal PKS ketosynthase (KS/KS alpha)
K05552  minimal PKS chain-length factor (CLF/KS beta)
K15886  multifunctional cyclase / dehydratase / O-methyltransferase
Genes
MBDMEBOL_003040 MEBOL_003041
MSAOMYCSP_12695 MYCSP_12700
MSALDSM43276_02542 DSM43276_02543
NBRO3I_007445 O3I_007450 O3I_015110 O3I_015115
NNONONO_c10800 NONO_c10810
NTPCRH09_08670 CRH09_08675
NOZDMB37_03465 DMB37_03470
NAHF5544_10730 F5544_10735 F5544_17020 F5544_17025 F5544_31060 F5544_31065
NIEKV110_14170 KV110_14175
NTCKHQ06_30030 KHQ06_30035
NYALTV02_09610 LTV02_09615
NGPLTT66_26670 LTT66_26675
SCOSCO5087(actIORF1) SCO5088(actIORF2)
SMASAVERM_2375(pks9-3) SAVERM_2376(pks9-4)
SSXSACTE_5202 SACTE_5203 SACTE_6346 SACTE_6347
SHYSHJG_1188 SHJG_1189
SHOSHJGH_1023 SHJGH_1024
SFASfla_5317 Sfla_5318
SBHSBI_06844 SBI_06845
SVESVEN_5977 SVEN_5981
SDVBN159_2470 BN159_2471 BN159_2514(tcmL) BN159_2515(tcmK)
SALSSLNWT_5121 SLNWT_5122
STRPF750_1305(whiE-KS) F750_1306(whiE-CLF)
SFISFUL_4002 SFUL_4003
SCIB446_13320 B446_13325
SALUDC74_6590 DC74_6591
SALLSAZ_34270 SAZ_34275
SLVSLIV_12945 SLIV_12950
SGUSGLAU_26355(tcmK) SGLAU_26360(tcmL) SGLAU_26370(tcmN) SGLAU_28975 SGLAU_28980
SVTSVTN_18775 SVTN_18780 SVTN_28595 SVTN_28600
STREGZL_p00214 GZL_p00215
SCWTU94_31825 TU94_31830
SLDT261_0018 T261_0019 T261_6830 T261_6831
SXISXIM_35600 SXIM_35610
STRMM444_36340
STRCAA958_14835 AA958_14840 AA958_30255 AA958_30260
SAMBSAM23877_0195(alpB) SAM23877_0196(alpA) SAM23877_0207(alpQ) SAM23877_0208(alpR) SAM23877_7465(alpR) SAM23877_7466(alpQ) SAM23877_7477(alpA) SAM23877_7478(alpB)
SPRISPRI_0266 SPRI_0267 SPRI_0278 SPRI_0280 SPRI_7073 SPRI_7075 SPRI_7086 SPRI_7087
SCZABE83_16240 ABE83_16245
SCXAS200_01090 AS200_01095
SRWTUE45_01296 TUE45_01297 TUE45_01569 TUE45_01570
STRFASR50_01170 ASR50_01175 ASR50_34265 ASR50_34270
SPAVSpa2297_32720 Spa2297_32725
SGSAVL59_46040 AVL59_46045
SGMGCM10017557_73750(fabF) GCM10017557_73760
SLSSLINC_6894 SLINC_6895
STRDNI25_18955 NI25_18960
SVUB1H20_09640 B1H20_09645
SPUNBFF78_19350 BFF78_19355
SLAUSLA_3073 SLA_3074
SALJSMD11_0675(actI1) SMD11_0676(actI2) SMD11_1144(actI2) SMD11_6044(actI1) SMD11_6045(actI2)
SLXSLAV_40240 SLAV_40245 SLAV_40510 SLAV_40515
SNZDC008_13175 DC008_13180
SGEDWG14_07359 DWG14_07360
SLKSLUN_33540 SLUN_33545
SDXC4B68_09595 C4B68_09600
SGDELQ87_00815 ELQ87_00820 ELQ87_08170 ELQ87_08175
SCYAEJ357_29965 EJ357_29970 EJ357_34775 EJ357_34780
SNQCP978_29745 CP978_29750
STIRDDW44_27775 DDW44_27780
SKACP970_02735 CP970_02740 CP970_39675 CP970_39680
SGZC0216_31850 C0216_31855
SNKCP967_10255 CP967_10260
SHAWCEB94_23570 CEB94_23575
SRKFGW37_04255 FGW37_04260 FGW37_16265 FGW37_16270
SFICEIZ62_03845 EIZ62_03850
SGALCP966_29070 CP966_29075
SSPODDQ41_07350 DDQ41_07355
SVRCP971_23915 CP971_23920
SFYGFH48_20450 GFH48_20455
SAQUEJC51_31355 EJC51_31360
SGFHEP81_07942 HEP81_07943
SCAVCVT27_18210 CVT27_18215
SRIMCP984_02375 CP984_02380
SCINCP977_28050 CP977_28055
SPACB1H29_00520 B1H29_00525 B1H29_36535 B1H29_36540
STSUB7R87_22035 B7R87_22040
SCHACP983_03645 CP983_03650 CP983_27885 CP983_27890
SGXH4W23_11745 H4W23_11750
SCOECP976_32910 CP976_32915
SBATG4Z16_23870 G4Z16_23875
SSPBCP982_19000 CP982_19005
SNFJYK04_04012 JYK04_04013
SPLACP981_11515 CP981_11520 CP981_34525 CP981_34530
STUIGCM10017668_38110(fabF) GCM10017668_38120
SCHFIPT68_15425 IPT68_15430
SHUNDWB77_05172 DWB77_05173
SHARHUT13_24890 HUT13_24895
SCYGS1361_29855 S1361_29860
SFEUIM697_37550 IM697_37555
SLIAHA039_05715 HA039_05720
SDWK7C20_32760 K7C20_32765
SCALI6J39_09855 I6J39_09860
SAUHSU9_027635 SU9_027640
SMOBJ7W19_30835 J7W19_30840
SPEUCGZ69_24310 CGZ69_24315
SROIIAG44_01160 IAG44_09610 IAG44_09615 IAG44_37955 IAG44_37960 IAG44_41430 IAG44_41435
SXNIAG42_35940 IAG42_35945 IAG42_36715 IAG42_36720
SHKJ2N69_28355 J2N69_28360
SANUK7396_04305 K7396_04310
SDDD9753_08525 D9753_08530
SACTDMT42_11840 DMT42_11845
SMAOCAG99_12130 CAG99_12135
SGOBtest1122_15630 test1122_15635
SINEKI385_25170 KI385_25175
SLFJEQ17_32810 JEQ17_32815
SDECL3078_20575 L3078_20580
KABB7C62_31265 B7C62_31270
KAUB6264_18490 B6264_18495
KITCFP65_4148 CFP65_4149
YIALO772_34315 LO772_34320
LMOIVV02_05815 VV02_05820
TFUTfu_1218 Tfu_1219
TALXFOF52_07540 FOF52_07545
TCUTcur_2772 Tcur_2773
AGRAAGRA3207_002370 AGRA3207_002371
SROSros_4150 Sros_4151
NOABKM31_22830 BKM31_22870
NCXNocox_12685 Nocox_12790
FRAFrancci3_4100 Francci3_4101 Francci3_4148
FRIFraEuI1c_1317 FraEuI1c_1318
SVISvir_33690 Svir_33700
AMDAMED_5434(fabF) AMED_5435(fabF)
AMNRAM_27700 RAM_27705
AMMAMES_5369(fabF) AMES_5370(fabF)
AMZB737_5369(fabF) B737_5370(fabF)
AORISD37_09325 SD37_09330 SD37_34200 SD37_34300
APRTMUY14_22560 MUY14_22565
PSEQAD006_30015 AD006_30020
PECQAD017_31230 AD017_31235
PHHAFB00_31125 AFB00_31130
SESPBN6_54860 BN6_54870
SSYIEKG83_16315 EKG83_16320 EKG83_32215 EKG83_32220
KALKALB_7830 KALB_7831
KPHYAOZ06_32110 AOZ06_32115
LEDBBK82_02450 BBK82_02455
AHMTL08_10465 TL08_10470
ACTIUA75_11090 UA75_11095
ACADUA74_11000 UA74_11005
AHGAHOG_10345 AHOG_10350
ACTAC1701_02395 C1701_02400
ALOCRK55091 CRK55092
PMADBAY61_07805 BAY61_07810
ACTUActkin_02486 Actkin_02487
STPStrop_2223 Strop_2224
MAUMicau_4018 Micau_4019
MILML5_4402 ML5_4403
MICHFJK98_03840 FJK98_03845
MSAGGCM10017556_44980 GCM10017556_44990(fabF)
VMAVAB18032_18170 VAB18032_18175
AMSAMIS_50420 AMIS_50430 AMIS_50640 AMIS_50650
ACTNL083_3108 L083_3109
AIHAiant_86560(fabF_2) Aiant_86570(fabF_3)
ACTRAsp14428_76450(fabF_2) Asp14428_76460(fabF_3)
PLKCIK06_11005 CIK06_11010
VERHUT12_05335 HUT12_05340
CATICS0771_73180(fabF_1) CS0771_73190
DVCDvina_17880 Dvina_17885
NHYJQS43_06230 JQS43_06235
CCAZCOUCH_16055 COUCH_16060
CAICaci_3546 Caci_3547 Caci_6485 Caci_6486
 » show all
Reference
1  [PMID:9609708]
  Authors
Bao W, Wendt-Pienkowski E, Hutchinson CR
  Title
Reconstitution of the iterative type II polyketide synthase for tetracenomycin F2 biosynthesis.
  Journal
Biochemistry 37:8132-8 (1998)
DOI:10.1021/bi980466i
Other DBs
ExplorEnz - The Enzyme Database: 2.3.1.235
IUBMB Enzyme Nomenclature: 2.3.1.235
ExPASy - ENZYME nomenclature database: 2.3.1.235
BRENDA, the Enzyme Database: 2.3.1.235
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