KEGG ENZYME: 2.4.2.60

ENZYME: 2.4.2.60

Entry

EC 2.4.2.60                 Enzyme

Name

cysteine-dependent adenosine diphosphate thiazole synthase;
THI4 (gene name) (ambiguous);
THI1 (gene name);
ADP-thiazole synthase

Class

Transferases;
Glycosyltransferases;
Pentosyltransferases

Sysname

NAD+:glycine ADP-D-ribosyltransferase (dehydroalanine-producing)

Reaction(IUBMB)

NAD+ + glycine + [ADP-thiazole synthase]-L-cysteine = nicotinamide + ADP-5-ethyl-4-methylthiazole-2-carboxylate + [ADP-thiazole synthase]-dehydroalanine + 3 H2O [RN:R10685]

Reaction(KEGG)

R10685

Substrate

NAD+ [CPD:C00003];
glycine [CPD:C00037];
[ADP-thiazole synthase]-L-cysteine

Product

nicotinamide [CPD:C00153];
ADP-5-ethyl-4-methylthiazole-2-carboxylate [CPD:C20784];
[ADP-thiazole synthase]-dehydroalanine;
H2O [CPD:C00001]

Comment

This iron dependent enzyme, found in fungi, plants, and some archaea, is involved in the thiamine phosphate biosynthesis pathway. It is a single turn-over enzyme since the cysteine residue is not regenerated in vivo [3]. The homologous enzyme in archaea (EC 2.4.2.59, sulfide-dependent adenosine diphosphate thiazole synthase) uses sulfide as sulfur donor.

History

EC 2.4.2.60 created 2018

Pathway

ec00730

Thiamine metabolism

ec01100

Metabolic pathways

Orthology

K03146

cysteine-dependent adenosine diphosphate thiazole synthase

Genes

ATH:	AT5G54770(THI1)

ALY:

9300430

CRB:

17876896

CSAT:

104726000 104731059 104763460

EUS:	EUTSA_v10013978mg

BRP:	103844930 103851941 103857026

BNA:	106346007 106347311 106366413 106371241 106402237 106420919 125601255

BOE:	106318832 106327201 106335239

RSZ:	108820570 108836615 108837050 108844151

THJ:

104817652

CPAP:

110810965

CIT:	102626594 102629135

CIC:	CICLE_v10008765mg CICLE_v10015727mg CICLE_v10024231mg

PVY:

116123427

MINC:

123202328 123225908

TCC:	18593644 18597097

GRA:	105769824 105773933 105780320

GHI:	107901923 107917921 107928645 107958248 107961959 121217764

GAB:	108453783 108482152 108486471

DZI:	111280819 111301627 111318798

EGR:

104450213

GMX:	100775614 100788953 100794288 547461(SC-04) 547462(SC-03)

GSJ:	114372051 114380540 114403613 114404912 114417056

PVU:	PHAVU_007G052900g PHAVU_011G139100g

VRA:	106754377 106755182

VAR:	108325373 108339541

VUN:	114168645 114192234

CCAJ:

109795024 109808173

APRC:

113872364

MTR:	11446281 25485243

TPRA:

123882686

CAM:	101491901 101507074 101511321 101512227

PSAT:

127097128

LJA:	Lj3g3v1010900.1(Lj3g3v1010900.1) Lj5g3v2060670.1(Lj5g3v2060670.1)

ADU:	107477997 107488645

AIP:	107630145 107643278

AHF:	112737808 112749911 112789941 112801227

LANG:

109331169 109332649 109348532 109351883

PCIN:

129286736 129298471 129298852 129315109

FVE:	101298688 101299651

RCN:	112168087 112201505 112201899

PPER:

18780719

PMUM:

103342510

PDUL:

117623858

MDM:	103429668 103448581

MSYL:

126589528 126615015

PXB:	103948770 103967326

ZJU:

107420469

MNT:

21395127

CSAV:

115717421

CSV:

101211553

CMO:

103501605

BHJ:

120091480

CMAX:

111483293 111487533

CMOS:

111438464 111448035

CPEP:

111783834 111785935 111788519

RCU:

8280736

JCU:

105632894

HBR:

110647144

MESC:

110601845 110611614

POP:	7470933 7479083 7485534

PEU:	105108187 105130169 105134848

PALZ:

118035853 118047445 118059587

JRE:	108983682 108993518 109022013

CILL:

122277821 122282819 122289841

QSU:	111989411 112016278

QLO:	115956034 115956545 115972735 115988494

TWL:	119988758 119988861

VVI:	100260728(THI1-1) 100265774(THI1-2)

VRI:	117909200 117923467

SLY:

101257192

SPEN:

107025837

SOT:

102598996

SSTN:

125856795

CANN:

107856341

NTA:	107797059(ThiI) 107798781

NSY:

104224143

NTO:

104087540

NAU:

109232136

INI:	109167290 109187908

ITR:	116005701 116028414

SIND:

105167970 105175375

OEU:	111383503 111389379

EGT:	105948772 105962248

SSPL:

121740783 121742722 121743409 121757713 121775363 121800880

SMIL:

130985758

APAN:

127247867 127249630

HAN:	110890327 110912864

ECAD:

122591292 122611091

LSV:	111877285 111914386

CCAV:

112507403 112514948

DCR:	108199579 108204812 108211831

CSIN:

114275070 114297269

RVL:	131322345 131330436

BVG:	104887771 104887775

SOE:

110795721

CQI:	110721030 110723750

ATRI:

130818300

MOF:

131145521

NNU:

104604883

MING:

122078253 122091022

TSS:

122640918

PSOM:

113279575 113284440 113287867 113288375

OSA:

4343443

DOSA:

Os07t0529600-01(Os07g0529600)

OBR:

102709384

OGL:

127779938

BDI:

100841709

ATS:

109771029

TDC:	119281869 119330895 119338169

TAES:

123066078 123148177 123160697 123169109

TUA:

125525971

LPER:

127313554 127333271

LRD:	124669313 124679092 124689497 124695681

SBI:	8063504 8078187

ZMA:	542714 542715

SITA:

101752840 101774400

SVS:	117842602 117853273

PVIR:

120671321 120704075

PHAI:

112889419

PDA:	103716822 103721429 120107672

EGU:	105046898 105051965

MUS:	103975582 103991030

ZOF:	121967276 122021558 122025718 122052496

DCT:

110108175

PEQ:

110031149

AOF:	109827745 109834756

NCOL:

116249223

ATR:

18435056

SMO:	SELMODRAFT_163429 SELMODRAFT_271002

PPP:	112273035 112273481 112275550 112276032 112276579

CRE:	CHLRE_04g214150v5

VCN:	VOLCADRAFT_74899 VOLCADRAFT_74900

MNG:	MNEG_15292 MNEG_7985

CSL:	COCSUDRAFT_14398

CVR:	CHLNCDRAFT_22703

APRO:

F751_1708

SCE:	YGR144W(THI4)

SEUB:

DI49_2107

SPAO:

SPAR_G03600

AGO:	AGOS_AAL137W

ERC:

Ecym_6012

KLA:	KLLA0_A00198g

KMX:	KLMA_40625(THI4)

LTH:	KLTH0B03916g

VPO:	Kpol_1050p31

ZRO:	ZYRO0G11726g

CGR:	CAGL0M01166g

NCS:	NCAS_0D01750(NCAS0D01750)

NDI:	NDAI_0C02860(NDAI0C02860)

TPF:	TPHA_0A04790(TPHA0A04790)

TBL:	TBLA_0B09390(TBLA0B09390) TBLA_0H00760(TBLA0H00760)

TGB:	HG536_0A04825 HG536_0F00230

TDL:	TDEL_0H00200(TDEL0H00200)

KNG:	KNAG_0E03150(KNAG0E03150)

ZMK:	HG535_0B00510

PPA:	PAS_chr3_0648

DHA:	DEHA2F16654g

PIC:	PICST_50942

PGU:	PGUG_04211

SPAA:

SPAPADRAFT_51398

LEL:	LELG_02871

CAL:	CAALFM_C305130CA(THI4)

CTP:	CTRG_02525

COT:	CORT_0C04770

CDU:	CD36_85100

YLI:	YALI0A09768g

CLU:	CLUG_04547

CLUS:

A9F13_11g02431

CAUR:

CJI96_0001634

SLB:	AWJ20_4223(THI4)

PKZ:	C5L36_0C00170

BNN:	FOA43_001955(STI35)

BBRX:

BRETT_001261(THI4)

OPA:	HPODL_03615

SLUD:

SCDLUD_003155

NCR:

NCU06110

NTE:	NEUTE1DRAFT95662(NEUTE1DRAFT_95662)

SMP:	SMAC_06409

PAN:	PODANSg1687

TTT:	THITE_2117402

MTM:	MYCTH_2315910

CTHR:

CTHT_0017160

MGR:

MGG_03098

PPEI:

PpBr36_09505

TMN:	UCRPA7_2510

SSCK:

SPSK_07738

FGR:	FGSG_02469

FPU:	FPSE_01936

FVR:	FVEG_09077

FOX:	FOXG_10428

NHE:	NECHADRAFT_61890

FFC:	NCS54_00810500

TRE:	TRIREDRAFT_68608(thi4)

TRR:	M419DRAFT_89757

MAW:

MAC_01186

MAJ:

MAA_05549

CMT:

CCM_01639

PLJ:	VFPFJ_09336

PTKZ:

JDV02_002359(THI4)

VAL:	VDBG_03908

VDA:	VDAG_01137

CFJ:	CFIO01_04759

CLUP:

CLUP02_16278

CHIG:

CH63R_12560

SAPO:

SAPIO_CDS2560

ELA:	UCREL1_6105

PFY:	PFICI_09188

SSL:	SS1G_09727

BFU:	BCIN_07g06010(Bcthi4)

MBE:

MBM_01288

PSCO:

LY89DRAFT_683526

GLZ:	GLAREA_02091

ANI:

AN3928.2

AFM:	AFUA_6G08360

ACT:	ACLA_082310

NFI:	NFIA_054010

AOR:	AO090003000090

ANG:	ANI_1_228094(An11g01630)

AFV:	AFLA_001605

ALUC:

AKAW2_80142S(THI4)

ACHE:

ACHE_80625S(THI4)

APUU:

APUU_40431S(THI4)

PCS:	Pc22g07960

PDP:	PDIP_32520

TMF:	EYB26_005937

TRG:	TRUGW13939_06486

CIM:	CIMG_01115

CPW:	CPC735_052570

URE:	UREG_01058

PBL:	PAAG_01125

PBN:	PADG_03708

ABE:

ARB_07940

TVE:

TRV_04098

AJE:	HCAG_05915

BGH:	BDBG_03342

PNO:	SNOG_05965

PTE:

PTT_08482

BZE:	COCCADRAFT_27023

BSC:	COCSADRAFT_38262

BOR:	COCMIDRAFT_30031

AALT:

CC77DRAFT_1021441

ZTR:	MYCGRDRAFT_44262

PFJ:	MYCFIDRAFT_211863

FFU:	CLAFUR5_09695

BCOM:

BAUCODRAFT_62660

NPA:	UCRNP2_7340

SPO:	SPBC26H8.01(thi2)

SOM:	SOMG_00517(thi2)

TVS:	TRAVEDRAFT_71517

DSQ:	DICSQDRAFT_134894

PCO:	PHACADRAFT_177254

SHS:	STEHIDRAFT_111603

HIR:	HETIRDRAFT_440039

PSQ:	PUNSTDRAFT_42416

ADL:	AURDEDRAFT_139075

FME:	FOMMEDRAFT_23696

GTR:	GLOTRDRAFT_77212

RSX:	RhiXN_04607

CCI:	CC1G_04976

ABP:	AGABI1DRAFT40074(AGABI1DRAFT_40074)

ABV:	AGABI2DRAFT72787(AGABI2DRAFT_72787)

PCUB:

JR316_0006998

MRR:	Moror_11852

E1B28_012388

SCM:	SCHCO_02490971(SCHCODRAFT_02490971)

CPUT:

CONPUDRAFT_104209

SLA:	SERLADRAFT_470126

WSE:	WALSEDRAFT_32181(CYPBP37)

WIC:	J056_001533

UMA:	UMAG_11400

PFP:	PFL1_06351

SGRA:

EX895_003350

MGL:

MGL_1314

MSYM:

MSY001_3139

PGR:	PGTG_01304

PTRC:

PtA15_7A55

MLR:	MELLADRAFT_53832

SMIN:

v1.2.003820.t1(symbB.v1.2.003820.t1)

SPAR:

SPRG_04526

GTT:	GUITHDRAFT_93981

» show all

Reference

1 [PMID:16912043]

Authors

Godoi PH, Galhardo RS, Luche DD, Van Sluys MA, Menck CF, Oliva G

Title

Structure of the thiazole biosynthetic enzyme THI1 from Arabidopsis thaliana.

Journal

J Biol Chem 281:30957-66 (2006)
DOI:10.1074/jbc.M604469200

Sequence

[ath:AT5G54770]

Reference

2 [PMID:22031445]

Authors

Chatterjee A, Abeydeera ND, Bale S, Pai PJ, Dorrestein PC, Russell DH, Ealick SE, Begley TP

Title

Saccharomyces cerevisiae THI4p is a suicide thiamine thiazole synthase.

Journal

Nature 478:542-6 (2011)
DOI:10.1038/nature10503

Sequence

[sce:YGR144W]

Reference

3 [PMID:26919468]

Authors

Zhang X, Eser BE, Chanani PK, Begley TP, Ealick SE

Title

Structural Basis for Iron-Mediated Sulfur Transfer in Archael and Yeast Thiazole Synthases.

Journal

Biochemistry 55:1826-38 (2016)
DOI:10.1021/acs.biochem.6b00030

Sequence

[sce:YGR144W]

Reference

4 [PMID:25348237]

Authors

Hwang S, Cordova B, Chavarria N, Elbanna D, McHugh S, Rojas J, Pfeiffer F, Maupin-Furlow JA

Title

Conserved active site cysteine residue of archaeal THI4 homolog is essential for thiamine biosynthesis in Haloferax volcanii.

Journal

BMC Microbiol 14:260 (2014)
DOI:10.1186/s12866-014-0260-0

Other DBs

ExplorEnz - The Enzyme Database:

2.4.2.60

IUBMB Enzyme Nomenclature:

2.4.2.60

ExPASy - ENZYME nomenclature database:

2.4.2.60

BRENDA, the Enzyme Database:

2.4.2.60

LinkDB

DBGET integrated database retrieval system