KEGG   ENZYME: 2.4.2.60
Entry
EC 2.4.2.60                 Enzyme                                 
Name
cysteine-dependent adenosine diphosphate thiazole synthase;
THI4 (gene name) (ambiguous);
THI1 (gene name);
ADP-thiazole synthase
Class
Transferases;
Glycosyltransferases;
Pentosyltransferases
Sysname
NAD+:glycine ADP-D-ribosyltransferase (dehydroalanine-producing)
Reaction(IUBMB)
NAD+ + glycine + [ADP-thiazole synthase]-L-cysteine = nicotinamide + ADP-5-ethyl-4-methylthiazole-2-carboxylate + [ADP-thiazole synthase]-dehydroalanine + 3 H2O [RN:R10685]
Reaction(KEGG)
R10685
Substrate
NAD+ [CPD:C00003];
glycine [CPD:C00037];
[ADP-thiazole synthase]-L-cysteine
Product
nicotinamide [CPD:C00153];
ADP-5-ethyl-4-methylthiazole-2-carboxylate [CPD:C20784];
[ADP-thiazole synthase]-dehydroalanine;
H2O [CPD:C00001]
Comment
This iron dependent enzyme, found in fungi, plants, and some archaea, is involved in the thiamine phosphate biosynthesis pathway. It is a single turn-over enzyme since the cysteine residue is not regenerated in vivo [3]. The homologous enzyme in archaea (EC 2.4.2.59, sulfide-dependent adenosine diphosphate thiazole synthase) uses sulfide as sulfur donor.
History
EC 2.4.2.60 created 2018
Pathway
ec00730  Thiamine metabolism
ec01100  Metabolic pathways
Orthology
K03146  cysteine-dependent adenosine diphosphate thiazole synthase
Genes
ATHAT5G54770(THI1)
ALY9300430
CRB17876896
CSAT104726000 104731059 104763460
EUSEUTSA_v10013978mg
BRP103844930 103851941 103857026
BNA106346007 106347311 106349644 106366413 106371241 106420919 106420920 106439989
BOE106318832 106327201 106335239
RSZ108820570 108836615 108837050 108844151
THJ104817652
CPAP110810965
CIT102626594 102629135
CICCICLE_v10008765mg CICLE_v10015727mg CICLE_v10024231mg
PVY116123427
MINC123202328 123225908
TCC18593644 18597097
GRA105769824 105773933 105780320
GHI107901923 107917921 107928645 107958248 107961959 121217764
GAB108453783 108482152 108486471
DZI111280819 111301627 111318798
EGR104450213
GMX100775614 100788953 100794288 547461(SC-04) 547462(SC-03)
GSJ114372051 114380540 114403613 114404912 114417056
PVUPHAVU_007G052900g PHAVU_011G139100g
VRA106754377 106755182
VAR108325373 108339541
VUN114168645 114192234
CCAJ109795024 109808173
APRC113872364
MTRMTR_1g105480 MTR_4g081130
CAM101491901 101507074 101511321 101512227
LJALj3g3v1010900.1(Lj3g3v1010900.1) Lj5g3v2060670.1(Lj5g3v2060670.1)
ADU107477997 107488645
AIP107630145 107643278
AHF112737808 112749911 112789941 112801227
LANG109331169 109332649 109348532 109351883
FVE101298688 101299651
RCN112168087 112201505 112201899
PPER18780719
PMUM103342510
PDUL117623858
MDM103429668 103448581
PXB103948770 103967326
ZJU107420469
MNT21395127
CSV101211553
CMO103501605
BHJ120091480
CMAX111483293 111487533
CMOS111438464 111448035
CPEP111783834 111785935 111788519
RCU8280736
JCU105632894
HBR110647144
MESC110601845 110611614
POP7470933 7479083 7485534
PEU105108187 105130169 105134848
PALZ118035853 118047445 118059587
JRE108983682 108993518 109022013
QSU111989411 111993711 112014551 112016278
QLO115956034 115956545 115972735 115988494
TWL119988758 119988861
VVI100260728(THI1-1) 100265774(THI1-2)
VRI117909200 117923467
SLY101257192
SPEN107025837
SOT102598996
CANN107856341
NTA107797059(ThiI) 107798781
NSY104224143
NTO104087540
NAU109232136
INI109167290 109187908
ITR116005701 116028414
SIND105167970 105175375
OEU111383503 111389379
EGT105948772 105962248
SSPL121740783 121742722 121743409 121757713 121775363 121800880
HAN110890327 110912864
ECAD122591292 122611091
LSV111877285 111914386
CCAV112507403 112514948
DCR108199579 108204812 108211831
CSIN114275070 114297269
BVG104887771 104887775
SOE110795721
CQI110721030 110723750
NNU104604883
MING122078253 122091022
TSS122640918
PSOM113279575 113284440 113287867 113288375
NCOL116249223
OSA4343443
DOSAOs07t0529600-01(Os07g0529600)
OBR102709384
BDI100841709
ATS109771029
TDC119281869 119330895 119338169
TAES123066078 123148177 123160697 123169109
SBI8063504 8078187
ZMA542714 542715
SITA101752840 101774400
SVS117842602 117853273
PVIR120671321 120704075
PHAI112889419
PDA103716822 103721429 120107672
EGU105046898 105051965
MUS103975582 103991030
DCT110108175
PEQ110031149
AOF109827745 109834756
ATR18435056
SMOSELMODRAFT_163429 SELMODRAFT_271002
PPP112273035 112273481 112275550 112276032 112276579
CRECHLRE_04g214150v5
VCNVOLCADRAFT_74899 VOLCADRAFT_74900
MNGMNEG_15292 MNEG_7985
CSLCOCSUDRAFT_14398
CVRCHLNCDRAFT_22703
APROF751_1708
SCEYGR144W(THI4)
SEUBDI49_2107
SPAOSPAR_G03600
AGOAGOS_AAL137W
ERCEcym_6012
KLAKLLA0_A00198g
KMXKLMA_40625(THI4)
LTHKLTH0B03916g
VPOKpol_1050p31
ZROZYRO0G11726g
CGRCAGL0M01166g
NCSNCAS_0D01750(NCAS0D01750)
NDINDAI_0C02860(NDAI0C02860)
TPFTPHA_0A04790(TPHA0A04790)
TBLTBLA_0B09390(TBLA0B09390) TBLA_0H00760(TBLA0H00760)
TDLTDEL_0H00200(TDEL0H00200)
TGBHG536_0A04825 HG536_0F00230
KNGKNAG_0E03150(KNAG0E03150)
ZMKHG535_0B00510
PPAPAS_chr3_0648
DHADEHA2F16654g
PICPICST_50942
PGUPGUG_04211
SPAASPAPADRAFT_51398
LELLELG_02871
CALCAALFM_C305130CA(THI4)
CTPCTRG_02525
COTCORT_0C04770
CDUCD36_85100
YLIYALI0A09768g
CLUCLUG_04547
CLUSA9F13_11g02431
CAURCJI97_003172
SLBAWJ20_4223(THI4)
PKZC5L36_0C00170
BNNFOA43_001955(STI35)
BBRXBRETT_001261(THI4)
OPAHPODL_03615
NCRNCU06110
NTENEUTE1DRAFT95662(NEUTE1DRAFT_95662)
SMPSMAC_06409
PANPODANSg1687
TTTTHITE_2117402
MTMMYCTH_2315910
CTHRCTHT_0017160
MGRMGG_03098
PPEIPpBr36_09505
TMNUCRPA7_2510
SSCKSPSK_07738
FGRFGSG_02469
FPUFPSE_01936
FVRFVEG_09077
FOXFOXG_10428
NHENECHADRAFT_61890
TRETRIREDRAFT_68608(thi4)
TRRM419DRAFT_89757
MAWMAC_01186
MAJMAA_05549
CMTCCM_01639
PLJVFPFJ_09336
VALVDBG_03908
VDAVDAG_01137
CFJCFIO01_04759
CHIGCH63R_12560
SAPOSAPIO_CDS2560
ELAUCREL1_6105
PFYPFICI_09188
SSLSS1G_09727
BFUBCIN_07g06010(Bcthi4)
MBEMBM_01288
PSCOLY89DRAFT_683526
GLZGLAREA_02091
ANIAN3928.2
AFMAFUA_6G08360
ACTACLA_082310
NFINFIA_054010
AORAO090003000090
ANGANI_1_228094(An11g01630)
AFVAFLA_001605
ALUCAKAW2_80142S(THI4)
ACHEACHE_80625S(THI4)
APUUAPUU_40431S(THI4)
PCSPc22g07960
PDPPDIP_32520
TMFPMAA_072920
TRGTRUGW13939_06486
CIMCIMG_01115
CPWCPC735_052570
UREUREG_01058
PBLPAAG_01125 PAAG_03817
PBNPADG_03708
ABEARB_07940
TVETRV_04098
AJEHCAG_05915
BGHBDBG_03342
PNOSNOG_05965
PTEPTT_08482
BZECOCCADRAFT_27023
BSCCOCSADRAFT_38262
BORCOCMIDRAFT_30031
AALTCC77DRAFT_1021441
ZTRMYCGRDRAFT_44262
PFJMYCFIDRAFT_211863
FFUCLAFUR5_09695
BCOMBAUCODRAFT_62660
NPAUCRNP2_7340
SPOSPBC26H8.01(thi2)
TVSTRAVEDRAFT_71517
DSQDICSQDRAFT_134894
PCOPHACADRAFT_177254
SHSSTEHIDRAFT_111603
HIRHETIRDRAFT_440039
PSQPUNSTDRAFT_42416
ADLAURDEDRAFT_139075
FMEFOMMEDRAFT_23696
GTRGLOTRDRAFT_77212
MRRMoror_11852
MOREE1B28_012388
CCICC1G_04976
SCMSCHCODRAFT_84820
ABPAGABI1DRAFT40074(AGABI1DRAFT_40074)
ABVAGABI2DRAFT72787(AGABI2DRAFT_72787)
CPUTCONPUDRAFT_104209
SLASERLADRAFT_470126
WSEWALSEDRAFT_32181(CYPBP37)
WICJ056_001533
UMAUMAG_11400
PFPPFL1_06351
MGLMGL_1314
MSYMMSY001_3139
PGRPGTG_01304
MLRMELLADRAFT_53832
SMINv1.2.003820.t1(symbB.v1.2.003820.t1)
SPARSPRG_04526
GTTGUITHDRAFT_93981
 » show all
Reference
1  [PMID:16912043]
  Authors
Godoi PH, Galhardo RS, Luche DD, Van Sluys MA, Menck CF, Oliva G
  Title
Structure of the thiazole biosynthetic enzyme THI1 from Arabidopsis thaliana.
  Journal
J Biol Chem 281:30957-66 (2006)
DOI:10.1074/jbc.M604469200
  Sequence
[ath:AT5G54770]
Reference
2  [PMID:22031445]
  Authors
Chatterjee A, Abeydeera ND, Bale S, Pai PJ, Dorrestein PC, Russell DH, Ealick SE, Begley TP
  Title
Saccharomyces cerevisiae THI4p is a suicide thiamine thiazole synthase.
  Journal
Nature 478:542-6 (2011)
DOI:10.1038/nature10503
  Sequence
[sce:YGR144W]
Reference
3  [PMID:26919468]
  Authors
Zhang X, Eser BE, Chanani PK, Begley TP, Ealick SE
  Title
Structural Basis for Iron-Mediated Sulfur Transfer in Archael and Yeast Thiazole  Synthases.
  Journal
Biochemistry 55:1826-38 (2016)
DOI:10.1021/acs.biochem.6b00030
  Sequence
[sce:YGR144W]
Reference
4  [PMID:25348237]
  Authors
Hwang S, Cordova B, Chavarria N, Elbanna D, McHugh S, Rojas J, Pfeiffer F, Maupin-Furlow JA
  Title
Conserved active site cysteine residue of archaeal THI4 homolog is essential for thiamine biosynthesis in Haloferax volcanii.
  Journal
BMC Microbiol 14:260 (2014)
DOI:10.1186/s12866-014-0260-0
Other DBs
ExplorEnz - The Enzyme Database: 2.4.2.60
IUBMB Enzyme Nomenclature: 2.4.2.60
ExPASy - ENZYME nomenclature database: 2.4.2.60
BRENDA, the Enzyme Database: 2.4.2.60
LinkDB

DBGET integrated database retrieval system