KEGG ENZYME: 3.2.2.5

ENZYME: 3.2.2.5

Entry

EC 3.2.2.5                  Enzyme

Name

NAD+ glycohydrolase;
NAD glycohydrolase;
nicotinamide adenine dinucleotide glycohydrolase;
beta-NAD+ glycohydrolase;
DPNase (ambiguous);
NAD hydrolase (ambiguous);
diphosphopyridine nucleosidase (ambiguous);
nicotinamide adenine dinucleotide nucleosidase (ambiguous);
NAD nucleosidase (ambiguous);
DPN hydrolase (ambiguous);
NADase (ambiguous);
nga (gene name);
NAD+ nucleosidase

Class

Hydrolases;
Glycosylases;
Hydrolysing N-glycosyl compounds

Sysname

NAD+ glycohydrolase

Reaction(IUBMB)

NAD+ + H2O = ADP-D-ribose + nicotinamide [RN:R00102]

Reaction(KEGG)

R00102

Substrate

NAD+ [CPD:C00003];
H2O [CPD:C00001]

Product

ADP-D-ribose [CPD:C00301];
nicotinamide [CPD:C00153]

Comment

This enzyme catalyses the hydrolysis of NAD+, without associated ADP-ribosyl cyclase activity (unlike the metazoan enzyme EC 3.2.2.6, bifunctional ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase). The enzyme from Group A streptococci has been implicated in the pathogenesis of diseases such as streptococcal toxic shock-like syndrome (STSS) and necrotizing fasciitis. The enzyme from the venom of the snake Agkistrodon acutus also catalyses EC 3.6.1.5, apyrase [3].

History

EC 3.2.2.5 created 1961, modified 2013

Pathway

ec00760

Nicotinate and nicotinamide metabolism

ec01100

Metabolic pathways

Orthology

K18153

NAD+ glycohydrolase

K19980

ADP-ribosyltransferase 2 / NAD+ glycohydrolase

K28299

NAD(P)(+) glycohydrolase toxin Tse6

Genes

MMU:	11871(Art2a) 11872(Art2b)

MCAL:

110298813

MPAH:

110336159

RNO:	293152(Art2b)

MCOC:

116100665 116101301

ANU:	117693579 117695831

ASYL:

127664680 127664858 127664865

MUN:

110550800

CGE:	100750708 100751294 100771938 100772218 100772803

MAUA:

101824175 101839372 121134115 121144298

PROB:

127227991 127227992

PLEU:

114698648 114698743 114698744 114706661 119086245

MFOT:

126488848

NGI:	103748863 103749849

HGL:

106007650

CPOC:

100714269 100717061

CCAN:

109678990

NCAR:

124958806

MMMA:

107135550

ITI:	101967560 101976814

OPI:

101536559

TUP:

102487179

GVR:

103607714

BACU:

103000152

BMUS:

118899688

MYB:

102252898

MYD:

102773420

MMYO:

118664685 118664687

MLF:	102425244 102438022 102438936

MDT:	132240834 132242078

MYUM:

139017312 139017313 139018192

EFUS:

103293570

MNA:

107533657

DRO:

112315853

SHON:

119002660

AJM:

119042110

PDIC:

114498648 114500760

PHAS:

123805524 123816978

MMF:

118627508

PPAM:

129072723

RFQ:

117031024

PALE:

102895427

PGIG:

120590522

PVP:

105303689

RAY:

107517326

SARA:

101541382

ETF:

123522055

DNM:

111761064

CPLA:

122545933

FCD:

110841647

ECLG:

EC036_25880

ECLI:

ECNIH5_12395

ECLZ:

LI64_12510

ECLA:

ECNIH3_12450

ECLC:

ECR091_12385

EEC:	EcWSU1_02673

ELG:	BH714_09110

EHU:	D5067_0009945

EQU:	OM418_12830

EDY:	F0320_12590

KOR:	AWR26_10875

KOT:	EH164_09985

KOB:	HF650_12920

KIN:	AB182_15040

MPHG:

MPHASIOC01_001702

EBC:	C2U52_25545

YPS:

YPTB3795

YPO:

BZ17_2790

YPI:	YpsIP31758_4019

YPB:

YPTS_3994

YPQ:

DJ40_2600

YPU:

BZ21_3132

YPR:

BZ20_2299

YPC:

BZ23_3420

YPF:

BZ19_3283

YEE:	YE5303_15111

YAL:

AT01_3152

SPE:

Spro_3017

SOF:	NCTC11214_03782

ERJ:	EJP617_B010

PAM:

PANA_2924

PEY:	EE896_11375 EE896_19530

PDIS:

D8B20_02745 D8B20_19815

PALF:

K6R05_21685

PPHO:

CTZ24_16115

PAMT:

IAI47_11530

PGZ:	C2E15_01190 C2E15_03645

MINT:

C7M51_00772(rhsA_2)

POJ:	PtoMrB4_30350

PAE:

PA0093

PAEV:

N297_99

PAEI:

N296_99

PAU:	PA14_01140

PAG:	PLES_00941

PAF:	PAM18_0095

PNC:	NCGM2_0098

PAEB:

NCGM1900_0088

PDK:	PADK2_00485

PSG:	G655_00480

PRP:	M062_00480

PAEP:

PA1S_00505

PAER:

PA1R_gp0491 PA1R_gp3607

PAEM:

U769_00495

PAES:

SCV20265_0095

PAEU:

BN889_00141

PAEC:

M802_99

PAEO:

M801_99

SECH:

B18_07420

PAP:	PSPA7_0167

PPAA:

B7D75_00515

PPU:

PP_3103

PPG:	PputGB1_2758

PPT:

PPS_2829

PPUN:

PP4_24620 PP4_24640

PPUD:

DW66_3085

PMON:

X969_13520

PMOT:

X970_13165

PPJ:	RK21_01838 RK21_01841

PALD:

LU682_015075

PSYR:

N018_23610 N018_25180 N018_25200

PSP:	PSPPH_2282 PSPPH_2819 PSPPH_5068

PSAV:

PSA3335_01220 PSA3335_24770

PAMG:

BKM19_028855

PFUV:

NLK61_13865

PFL:

PFL_6209

PPRC:

PFLCHA0_c61700

PPRO:

PPC_6163

PCG:	AXG94_14815

PMED:

E3Z27_27855

PSOS:

POS17_6171

PVK:	EPZ47_29230

PEZ:	HWQ56_04915

PBZ:	GN234_18820 GN234_18830

PSEP:

C4K39_0174 C4K39_3563

PPII:

QL104_00930 QL104_19260

PVW:	HU752_000430

PALV:

KSS97_00365

PTW:	TUM18999_29980

PASI:

LG197_19740

PSOA:

PSm6_52880

PTAI:

ICN73_20160

PSJY:

AA098_13375

PSKU:

KUIN1_23370

PSIV:

LZ023_33890

MMAI:

sS8_2269 sS8_3581

RSO:

RSc3426

RSE:

F504_3474

RSC:	RCFBP_10026

RSL:	RPSI07_0020

RSN:	RSPO_c00018

BTEI:

WS51_08385

BSTL:

BBJ41_25115

BARI:

NLX30_14130

BGF:	BC1003_5766

PMEG:

FNZ07_27120

TCAR:

U0034_07425 U0034_10385

CARE:

LT85_3876

CPRA:

CPter91_2340

MASS:

CR152_05325

MASY:

DPH57_04905 DPH57_04925

MANC:

IV454_05125 IV454_11395

MASZ:

C9I28_17075

MPLI:

E1742_04375

TCT:	PX653_05700

TMJ:	P0M04_28865

EHB:	E7V67_014940

POQ:	KZX46_03685 KZX46_18530

PMEA:

KTC28_14930 KTC28_19450

SPY:	SPy_0165(nga)

SPZ:	M5005_Spy0139(nga)

SPYM:

M1GAS476_0178(nga)

SPYA:

A20_0190(nga)

SPM:	spyM18_0163(nga)

SPG:	SpyM3_0128(nga)

SPS:

SPs0130

SPH:	MGAS10270_Spy0141(nga)

SPI:	MGAS10750_Spy0145(nga)

SPJ:	MGAS2096_Spy0146(nga)

SPK:	MGAS9429_Spy0141(nga)

SPF:	SpyM50134(nga)

SPA:	M6_Spy0186

SPB:	M28_Spy0137(nga)

STG:	MGAS15252_0175(nga)

STX:	MGAS1882_0175(nga)

SOZ:	Spy49_0144(nga)

STZ:	SPYALAB49_000178

SPYH:

L897_00945

SPYO:

STAB901_00975

SEU:

SEQ_0311

SDS:	SDEG_2029(nga)

SDG:	SDE12394_10265

SDA:	GGS_1869(nga)

SDC:	SDSE_2125(nga)

SDQ:	SDSE167_2131(nga)

SCAI:

NCTC12191_01452

» show all

Reference

1 [PMID:4322210]

Authors

Fehrenbach FJ

Title

[Purification and crystallization of NAD glycohydrolase of C streptococci].

Journal

Eur J Biochem 18:94-102 (1971)
DOI:10.1111/j.1432-1033.1971.tb01219.x

Reference

2 [PMID:236282]

Authors

Grushoff PS, Shany S, Bernheimer AW

Title

Purification and properties of streptococcal nicotinamide adenine dinucleotide glycohydrolase.

Journal

J Bacteriol 122:599-605 (1975)
DOI:10.1128/JB.122.2.599-605.1975

Reference

3 [PMID:18952139]

Authors

Zhang L, Xu X, Luo Z, Shen D, Wu H

Title

Identification of an unusual AT(D)Pase-like activity in multifunctional NAD glycohydrolase from the venom of Agkistrodon acutus.

Journal

Biochimie 91:240-51 (2009)
DOI:10.1016/j.biochi.2008.09.003

Reference

4 [PMID:20018886]

Authors

Ghosh J, Anderson PJ, Chandrasekaran S, Caparon MG

Title

Characterization of Streptococcus pyogenes beta-NAD+ glycohydrolase: re-evaluation of enzymatic properties associated with pathogenesis.

Journal

J Biol Chem 285:5683-94 (2010)
DOI:10.1074/jbc.M109.070300

Reference

5 [PMID:21300288]

Authors

Smith CL, Ghosh J, Elam JS, Pinkner JS, Hultgren SJ, Caparon MG, Ellenberger T

Title

Structural basis of Streptococcus pyogenes immunity to its NAD+ glycohydrolase toxin.

Journal

Structure 19:192-202 (2011)
DOI:10.1016/j.str.2010.12.013

Other DBs

ExplorEnz - The Enzyme Database:

3.2.2.5

IUBMB Enzyme Nomenclature:

3.2.2.5

ExPASy - ENZYME nomenclature database:

3.2.2.5

BRENDA, the Enzyme Database:

3.2.2.5

CAS:

9025-46-1

LinkDB

DBGET integrated database retrieval system