KEGG   ENZYME: 3.2.2.5
Entry
EC 3.2.2.5                  Enzyme                                 
Name
NAD+ glycohydrolase;
NAD glycohydrolase;
nicotinamide adenine dinucleotide glycohydrolase;
beta-NAD+ glycohydrolase;
DPNase (ambiguous);
NAD hydrolase (ambiguous);
diphosphopyridine nucleosidase (ambiguous);
nicotinamide adenine dinucleotide nucleosidase (ambiguous);
NAD nucleosidase (ambiguous);
DPN hydrolase (ambiguous);
NADase (ambiguous);
nga (gene name);
NAD+ nucleosidase
Class
Hydrolases;
Glycosylases;
Hydrolysing N-glycosyl compounds
Sysname
NAD+ glycohydrolase
Reaction(IUBMB)
NAD+ + H2O = ADP-D-ribose + nicotinamide [RN:R00102]
Reaction(KEGG)
R00102
Substrate
NAD+ [CPD:C00003];
H2O [CPD:C00001]
Product
ADP-D-ribose [CPD:C00301];
nicotinamide [CPD:C00153]
Comment
This enzyme catalyses the hydrolysis of NAD+, without associated ADP-ribosyl cyclase activity (unlike the metazoan enzyme EC 3.2.2.6, bifunctional ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase). The enzyme from Group A streptococci has been implicated in the pathogenesis of diseases such as streptococcal toxic shock-like syndrome (STSS) and necrotizing fasciitis. The enzyme from the venom of the snake Agkistrodon acutus also catalyses EC 3.6.1.5, apyrase [3].
History
EC 3.2.2.5 created 1961, modified 2013
Pathway
ec00760  Nicotinate and nicotinamide metabolism
ec01100  Metabolic pathways
Orthology
K18153  NAD+ glycohydrolase
K19980  ADP-ribosyltransferase 2 / NAD+ glycohydrolase
Genes
MMU: 11871(Art2a) 11872(Art2b)
MCAL: 110298812 110298813
MPAH: 110336159
RNO: 293152(Art2b)
MCOC: 116100665 116101301
MUN: 110550800
CGE: 100750708 100751294 100771938 100772218 100772803
MAUA: 101824175 101839372 121134115 121144298
PLEU: 114698648 114698743 114698744 114706661 119086245
NGI: 103748863 103749849
HGL: 106007650
CPOC: 100714269 100717061
CCAN: 109678990
NCAR: 124958806
OPI: 101536559
TUP: 102487179
BACU: 103000152
MYB: 102252898
MYD: 102773420
MMYO: 118664685 118664687
MLF: 102425244 102438022 102438936
MNA: 107533657
DRO: 112315853
SHON: 119002660
AJM: 119042110
PDIC: 114498648 114500760
PHAS: 123805524 123816978
MMF: 118627508
RFQ: 117031024
PALE: 102895427
PGIG: 120590522
PVP: 105303689
SARA: 101541382
DNM: 111761064
FCD: 110841647
SPY: SPy_0165(nga)
SPZ: M5005_Spy0139(nga)
SPYM: M1GAS476_0178(nga)
SPYA: A20_0190(nga)
SPM: spyM18_0163(nga)
SPG: SpyM3_0128(nga)
SPS: SPs0130
SPH: MGAS10270_Spy0141(nga)
SPI: MGAS10750_Spy0145(nga)
SPJ: MGAS2096_Spy0146(nga)
SPK: MGAS9429_Spy0141(nga)
SPF: SpyM50134(nga)
SPA: M6_Spy0186
SPB: M28_Spy0137(nga)
STG: MGAS15252_0175(nga)
STX: MGAS1882_0175(nga)
SOZ: Spy49_0144(nga)
STZ: SPYALAB49_000178
SPYH: L897_00945
SPYO: STAB901_00975
SEU: SEQ_0311
SDS: SDEG_2029(nga)
SDG: SDE12394_10265
SDA: GGS_1869(nga)
SDC: SDSE_2125(nga)
SDQ: SDSE167_2131(nga)
SCAI: NCTC12191_01452
 » show all
Reference
1  [PMID:4322210]
  Authors
Fehrenbach FJ
  Title
[Purification and crystallization of NAD glycohydrolase of C streptococci].
  Journal
Eur J Biochem 18:94-102 (1971)
DOI:10.1111/j.1432-1033.1971.tb01219.x
Reference
2  [PMID:236282]
  Authors
Grushoff PS, Shany S, Bernheimer AW
  Title
Purification and properties of streptococcal nicotinamide adenine dinucleotide glycohydrolase.
  Journal
J Bacteriol 122:599-605 (1975)
DOI:10.1128/JB.122.2.599-605.1975
Reference
3  [PMID:18952139]
  Authors
Zhang L, Xu X, Luo Z, Shen D, Wu H
  Title
Identification of an unusual AT(D)Pase-like activity in multifunctional NAD glycohydrolase from the venom of Agkistrodon acutus.
  Journal
Biochimie 91:240-51 (2009)
DOI:10.1016/j.biochi.2008.09.003
Reference
4  [PMID:20018886]
  Authors
Ghosh J, Anderson PJ, Chandrasekaran S, Caparon MG
  Title
Characterization of Streptococcus pyogenes beta-NAD+ glycohydrolase: re-evaluation of enzymatic properties associated with pathogenesis.
  Journal
J Biol Chem 285:5683-94 (2010)
DOI:10.1074/jbc.M109.070300
Reference
5  [PMID:21300288]
  Authors
Smith CL, Ghosh J, Elam JS, Pinkner JS, Hultgren SJ, Caparon MG, Ellenberger T
  Title
Structural basis of Streptococcus pyogenes immunity to its NAD+ glycohydrolase toxin.
  Journal
Structure 19:192-202 (2011)
DOI:10.1016/j.str.2010.12.013
Other DBs
ExplorEnz - The Enzyme Database: 3.2.2.5
IUBMB Enzyme Nomenclature: 3.2.2.5
ExPASy - ENZYME nomenclature database: 3.2.2.5
BRENDA, the Enzyme Database: 3.2.2.5
CAS: 9025-46-1
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