Entry
Name
methanogen homoaconitase;
methanogen HACN
Class
Lyases;
Carbon-oxygen lyases;
Hydro-lyases
BRITE hierarchy
Sysname
(R)-2-hydroxybutane-1,2,4-tricarboxylate hydro-lyase [(1R,2S)-1-hydroxybutane-1,2,4-tricarboxylate-forming]
Reaction(IUBMB)
(R)-2-hydroxybutane-1,2,4-tricarboxylate = (1R,2S)-1-hydroxybutane-1,2,4-tricarboxylate (overall reaction) [RN:
R09720 ];
(1a) (R)-2-hydroxybutane-1,2,4-tricarboxylate = (Z)-but-1-ene-1,2,4-tricarboxylate + H2O [RN:
R03444 ];
(1b) (Z)-but-1-ene-1,2,4-tricarboxylate + H2O = (1R,2S)-1-hydroxybutane-1,2,4-tricarboxylate [RN:
R04371 ]
Reaction(KEGG)
Substrate
(R)-2-hydroxybutane-1,2,4-tricarboxylate [CPD:
C01251 ];
(Z)-but-1-ene-1,2,4-tricarboxylate [CPD:
C04002 ];
H2O [CPD:
C00001 ]
Product
(1R,2S)-1-hydroxybutane-1,2,4-tricarboxylate [CPD:
C05662 ];
(Z)-but-1-ene-1,2,4-tricarboxylate [CPD:
C04002 ];
H2O [CPD:
C00001 ]
Comment
This enzyme catalyses several reactions in the pathway of coenzyme-B biosynthesis in methanogenic archaea. Requires a [4Fe-4S] cluster for activity. In contrast to EC
4.2.1.36 , homoaconitate hydratase, this enzyme can catalyse both the dehydration of (R)-homocitrate to form cis-homoaconitate and the subsequent hydration reaction that forms homoisocitrate. In addition to cis-homoaconitate, the enzyme can also catalyse the hydration of the physiological substrates dihomoaconitate and trihomoaconitate as well as the non-physiological substrate tetrahomoaconitate. cis-Aconitate and threo-DL-isocitrate cannot act as substrates, and (S)-homocitrate and trans-homoaconitate act as inhibitors of the enzyme.
History
EC 4.2.1.114 created 2009
Pathway
ec01110 Biosynthesis of secondary metabolites
ec01120 Microbial metabolism in diverse environments
Orthology
K16792 methanogen homoaconitase large subunit
K16793 methanogen homoaconitase small subunit
Genes
SACV : SPACI_025170(dmdB_1)
RRS : RoseRS_1053 RoseRS_1056
CHL : Chy400_0354 Chy400_0355
LEK : hrd7_27860(leuC_2) hrd7_27870(leuD_2)
DRA : DR_1610 DR_1614(leuD)
DDR : Deide_13460 Deide_13470
DMR : Deima_1353 Deima_1355
DGO : DGo_CA1306 DGo_CA1308
DPD : Deipe_2762 Deipe_2764
DSW : QR90_07810 QR90_09055
DCH : SY84_02050 SY84_02060
DPU : SU48_08705 SU48_08715
DEZ : DKM44_08505 DKM44_08520
DWU : DVJ83_05635 DVJ83_05640
DEIN : DAAJ005_09955 DAAJ005_11930
DGA : DEGR_14160(leuC1) DEGR_14180(leuD1)
DPH : EHF33_03450 EHF33_05185
DRB : N0D28_07410 N0D28_11260
DAH : DAETH_14730(leuC1) DAETH_14760(leuD1)
DAQU : M8445_12290 M8445_12300
DTAE : LAJ19_06385 LAJ19_06390
DMB : E5F05_13860 E5F05_13870
DSC : ABOD76_16820 ABOD76_16825
TTJ : TTHA1910(TTHA1910) TTHA1911(TTHA1911)
TTS : Ththe16_1910 Ththe16_1911
TTL : TtJL18_1992 TtJL18_1993
TSC : TSC_c01880(hacA) TSC_c01890(hacB)
THC : TCCBUS3UF1_21060 TCCBUS3UF1_21070
TOS : Theos_1936 Theos_1937
TPAR : AV541_01240 AV541_01245
TBC : A0O31_00873 A0O31_00874
TANT : KNN15_05110 KNN15_05115
MRE : K649_08895 K649_08900
MTAI : Mtai_v1c23260 Mtai_v1c23270
MSV : Mesil_0441 Mesil_0442
OPR : Ocepr_1780 Ocepr_1781
MHD : Marky_0671 Marky_0672
SCOR : J3U87_19130 J3U87_19135
MFE : Mefer_0048 Mefer_1231
MVU : Metvu_0677 Metvu_0864 Metvu_1303
MFS : MFS40622_0030 MFS40622_0660 MFS40622_1618
MIF : Metin_0516 Metin_1437
MJH : JH146_0454 JH146_0954
MESG : MLAUSG7_0025(hacB) MLAUSG7_0432(hacA)
MESA : MLASG1_0006(hacA) MLASG1_1205(hacB)
MIG : Metig_0050 Metig_0807 Metig_1570
MMQ : MmarC5_0098 MmarC5_1257
MMX : MmarC6_0529 MmarC6_1194
MMZ : MmarC7_0724 MmarC7_1379
MMAK : MMKA1_04000(leuD) MMKA1_17030(leuD)
MMAO : MMOS7_03940(leuD) MMOS7_15860(leuD)
MMAD : MMJJ_06580(dmdB_1) MMJJ_13590(dmdA_1)
MVN : Mevan_0789 Mevan_1368
MOK : Metok_0062 Metok_0567
METF : CFE53_04745 CFE53_05095
MMG : MTBMA_c02160(leuC1) MTBMA_c12270(leuD1)
MWO : MWSIV6_0177(hacA) MWSIV6_1206(hacB)
MTEE : MTTB_05450 MTTB_13810
METC : MTCT_0743 MTCT_1490
METE : tca_00796(DmdB_1) tca_01581(dmdA_2)
METZ : METMT2_0903 METMT2_1470
METK : FVF72_03470 FVF72_07395
MTHM : FZP57_00775 FZP57_05725
METJ : FZP68_00480 FZP68_05785
MST : Msp_0374(leuD2) Msp_1100(leuC2)
METB : AW729_05200 AW729_10315
MEIS : PXD04_02105(hacA) PXD04_05680(hacB)
MRU : mru_0384(aksD) mru_1689(aksE)
MEB : Abm4_0180(aksD) Abm4_0514(aksE)
MMIL : sm9_1773(aksE) sm9_2161(aksD)
MEYE : TL18_01065 TL18_07930
MARO : MarbSA_13800 MarbSA_18800
METV : K4897_04160 K4897_07245
MEL : Metbo_1944 Metbo_2070
MEW : MSWAN_0570 MSWAN_0676
METH : MBMB1_0289(leuC2) MBMB1_0696(leuD1)
MFC : BRM9_1499(aksE) BRM9_1986(aksD)
MFI : DSM1535_0178(leuC2) DSM1535_2193(leuD1)
MCUB : MCBB_0567(hacA) MCBB_0707(hacB)
MSUB : BK009_01700 BK009_11610
METN : BK008_07185 BK008_09225
METT : CIT01_01955 CIT01_06840
METO : CIT02_03290 CIT02_05440
MEME : HYG87_03480 HYG87_07560
MFEG : GCM10025860_11490 GCM10025860_17660
MEER : NKF70_09715(hacA) NKF70_10465(hacB)
MKA : MK1206(leuD_2) MK1208(leuC_2)
MBA : Mbar_A0244 Mbar_A1961 Mbar_A2270
MBY : MSBRM_0704 MSBRM_1355 MSBRM_2710
MBW : MSBRW_0751 MSBRW_2476 MSBRW_2776
MBAR : MSBR2_1054 MSBR2_1672 MSBR2_2666
MBAK : MSBR3_1018 MSBR3_1673 MSBR3_2760
MAC : MA_1223(leuC) MA_3085(leuC) MA_3751(leuD)
MMAZ : MmTuc01_0417 MmTuc01_0660
MMJ : MSMAS_0664 MSMAS_0894
MMAC : MSMAC_0666 MSMAC_0883
MVC : MSVAZ_0690 MSVAZ_1170 MSVAZ_2221
MEK : MSKOL_0668 MSKOL_1122 MSKOL_2220
MLS : MSLAZ_2100 MSLAZ_2434
METM : MSMTP_0731 MSMTP_2281
MEF : MSWH1_0826 MSWH1_0957
MEQ : MSWHS_0885 MSWHS_1027
MSJ : MSSAC_2956 MSSAC_3368 MSSAC_3559
MSZ : MSSIH_2594 MSSIH_2894 MSSIH_3101
MSW : MSSIT_2634 MSSIT_2938 MSSIT_3158
MTHR : MSTHT_2374 MSTHT_2523
MTHE : MSTHC_0762 MSTHC_0906
MHOR : MSHOH_0786 MSHOH_1131
MFZ : AOB57_002735 AOB57_003550
MBU : Mbur_1578 Mbur_2263(hacA)
MMET : MCMEM_0412 MCMEM_2089
MELO : J7W08_00085 J7W08_09410
MHAZ : BHR79_01390 BHR79_02815
MPOT : BKM01_05485 BKM01_07045
MEV : Metev_1312 Metev_1371
MZH : Mzhil_0725 Mzhil_1660
MZI : HWN40_08640 HWN40_10575
MMAV : RE476_01605 RE476_12705
MSEB : RE474_09445 RE474_11260
MHZ : Metho_0481 Metho_0694
MEHF : MmiHf6_03250(dmdB_1) MmiHf6_03300(leuC_1)
MEES : MmiEs2_06120(dmdB_1) MmiEs2_06150(leuC_1)
MCJ : MCON_0444(leuC1) MCON_1922(leuD1)
MRTJ : KHC33_05410 KHC33_05415
MEM : Memar_0634 Memar_0635
MBG : BN140_1716(leuD3) BN140_1717(leuC3)
MCHK : MchiMG62_07640 MchiMG62_07650
MSUM : OH143_09890 OH143_09895
MRC : R6Y96_02540 R6Y96_02545
MEFW : F1737_00605 F1737_00610
MAQE : RJ40_05975 RJ40_05980
MFK : E2N92_06105 E2N92_06110
MOU : OU421_03655 OU421_03660
MESB : L1S32_07255 L1S32_07260
MFO : Metfor_2591 Metfor_2592
MEZ : Mtc_1558(leuD-2) Mtc_1559(leuC-2)
RCI : RCIX2645(leuD-2) RCIX2646(leuC-2)
MEUZ : KRP56_01645 KRP56_01650 KRP56_05135
» show all
Taxonomy
Reference
Authors
Drevland RM, Jia Y, Palmer DR, Graham DE
Title
Methanogen homoaconitase catalyzes both hydrolyase reactions in coenzyme B biosynthesis.
Journal
Sequence
Other DBs
ExPASy - ENZYME nomenclature database: 4.2.1.114
LinkDB
All DBs