KEGG   ENZYME: 4.2.3.141
Entry
EC 4.2.3.141                Enzyme                                 
Name
sclareol synthase;
SS
Class
Lyases;
Carbon-oxygen lyases;
Acting on phosphates
Sysname
(13E)-8alpha-hydroxylabd-13-en-15-yl-diphosphate-lyase (sclareol-forming)
Reaction(IUBMB)
(13E)-8alpha-hydroxylabd-13-en-15-yl diphosphate + H2O = sclareol + diphosphate [RN:R10241]
Reaction(KEGG)
R10241
Substrate
(13E)-8alpha-hydroxylabd-13-en-15-yl diphosphate [CPD:C20270];
H2O [CPD:C00001]
Product
sclareol [CPD:C09183];
diphosphate [CPD:C00013]
Comment
Isolated from the plant Salvia sclarea (clary sage). Originally thought to be synthesized in one step from geranylgeranyl diphosphate it is now known to require two enzymes, EC 4.2.1.133, copal-8-ol diphosphate synthase and EC 4.2.3.141, sclareol synthase. Sclareol is used in perfumery.
History
EC 4.2.3.141 created 2013, modified 2017
Pathway
ec00904  Diterpenoid biosynthesis
ec01110  Biosynthesis of secondary metabolites
Orthology
K20519  sclareol synthase
Reference
1  [PMID:22834731]
  Authors
Caniard A, Zerbe P, Legrand S, Cohade A, Valot N, Magnard JL, Bohlmann J, Legendre L
  Title
Discovery and functional characterization of two diterpene synthases for sclareol biosynthesis in Salvia sclarea (L.) and their relevance for perfume manufacture.
  Journal
BMC Plant Biol 12:119 (2012)
DOI:10.1186/1471-2229-12-119
  Sequence
[ag:AFU61898]
Other DBs
ExplorEnz - The Enzyme Database: 4.2.3.141
IUBMB Enzyme Nomenclature: 4.2.3.141
ExPASy - ENZYME nomenclature database: 4.2.3.141
BRENDA, the Enzyme Database: 4.2.3.141
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