KEGG   ORTHOLOGY: K18364
Entry
K18364                      KO                                     
Symbol
bphH, xylJ, tesE
Name
2-oxopent-4-enoate/cis-2-oxohex-4-enoate hydratase [EC:4.2.1.80 4.2.1.132]
Pathway
map00362  Benzoate degradation
map00621  Dioxin degradation
map00622  Xylene degradation
map01100  Metabolic pathways
map01120  Microbial metabolism in diverse environments
map01220  Degradation of aromatic compounds
Module
M00569  Catechol meta-cleavage, catechol => acetyl-CoA / 4-methylcatechol => propanoyl-CoA
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09111 Xenobiotics biodegradation and metabolism
   00362 Benzoate degradation
    K18364  bphH, xylJ, tesE; 2-oxopent-4-enoate/cis-2-oxohex-4-enoate hydratase
   00622 Xylene degradation
    K18364  bphH, xylJ, tesE; 2-oxopent-4-enoate/cis-2-oxohex-4-enoate hydratase
   00621 Dioxin degradation
    K18364  bphH, xylJ, tesE; 2-oxopent-4-enoate/cis-2-oxohex-4-enoate hydratase
Enzymes [BR:ko01000]
 4. Lyases
  4.2  Carbon-oxygen lyases
   4.2.1  Hydro-lyases
    4.2.1.80  2-oxopent-4-enoate hydratase
     K18364  bphH, xylJ, tesE; 2-oxopent-4-enoate/cis-2-oxohex-4-enoate hydratase
    4.2.1.132  2-hydroxyhexa-2,4-dienoate hydratase
     K18364  bphH, xylJ, tesE; 2-oxopent-4-enoate/cis-2-oxohex-4-enoate hydratase
Dioxygenases [br01602.html]
 Dioxygenases in aromatic ring cleavage
  K18364
Other DBs
RN: R02601 R05864
GO: 0008684 0034856
Genes
PGE: LG71_12240
PLUM: A4R40_11035
PAKH: B0X70_11350
PLUI: CE143_11265
PSD: DSC_09160 DSC_09365
RGL: CS053_01760
RTH: LRK53_10275(dmpE)
RBD: ALSL_1080
VAF: D1115_22450
PRE: PCA10_13820 PCA10_p0350(carD)
PPSE: BN5_3620(nahL)
PCQ: PcP3B5_25700(tesE_1)
PPI: ND086(nahL)
PPUT: L483_14065
PPUN: PP4_27260
PPUU: PputUW4_01660(mhpD)
PKC: PKB_4239(xylJ)
PSET: THL1_1433
PTAE: NCTC10697_02057(xylJ)
AVN: Avin_08670(xylJ) Avin_30610(lapE)
AVL: AvCA_08670(xylJ) AvCA_30610(lapE)
AVD: AvCA6_08670(xylJ) AvCA6_30610(lapE)
HPEG: EAO82_00555(dmpE) EAO82_09425(dmpE)
HAES: LO767_17670(dmpE)
MARJ: MARI_05440(xylJ)
SPL: Spea_2118
SHL: Shal_2090
SWD: Swoo_1392
PHA: PSHAa2141(xylJ)
PTN: PTRA_a2594(bphH)
PNG: PNIG_a2788(bphH)
CYQ: Q91_0508
CYY: CPC19_10515(dmpE)
BLEP: AL038_01300(dmpE)
AEH: Mlg_2460
APAC: S7S_06990
MGEO: CFI10_00425(dmpE) CFI10_04960(dmpE)
NCU: F0U83_03045(dmpE)
NJP: NEJAP_0671(mhpD)
NIK: F5I99_10105(dmpE) F5I99_10520(dmpE)
AJP: AMJAP_2999(mhpD)
ASIM: FE240_03500(dmpE)
TBN: TBH_C0928
GPB: HDN1F_27090(tesE)
NZO: SAMEA4504057_0450(xylJ)
NCI: NCTC10296_00911(xylJ_1)
NWD: H3L96_04745(dmpE)
NDU: LVJ88_00610(dmpE)
PTES: JQU52_04880(dmpE)
USU: LVJ78_03680(dmpE)
RPI: Rpic_4620
RIN: ACS15_4354(dmpE)
REH: H16_B0548(mhpD1) H16_B0597(bphH)
CNC: CNE_2c04940(mhpD) CNE_2c05420(xylJ2) CNE_BB1p11990(xylJ1) CNE_BB1p13280(xylJ2)
RME: Rmet_1321(xylJ)
CTI: RALTA_B0401(mhpD)
COX: E0W60_07775(dmpE) E0W60_08025(dmpE)
BCJ: BCAM1613
BCEN: DM39_3383(dmpE) DM39_4834(dmpE)
BCEO: I35_5473
BCED: DM42_4993(dmpE) DM42_6345(dmpE)
BMEC: WJ16_26790
BANN: JFN94_19125(dmpE) JFN94_25650(dmpE)
BURK: DM992_32625(dmpE)
BXE: Bxe_C1189(bphH)
BXB: DR64_8616(dmpE)
BFN: OI25_4589(dmpE)
PARB: CJU94_37870(dmpE) CJU94_38115(dmpE)
PMEG: FNZ07_21165(dmpE)
CABA: SBC2_49020(tesE)
LIMN: HKT17_03820(dmpE)
KGY: EHF36_01675(dmpE) EHF36_04885(dmpE)
PNA: Pnap_4144
AJS: Ajs_0222
AMON: H9L24_14185(dmpE)
VEI: Veis_2785
VBO: CKY39_28505(dmpE)
CTES: O987_07740
COF: FOZ74_08305(dmpE)
HPSE: HPF_01870(xylJ)
HYN: F9K07_02750(dmpE)
HTN: KI616_01965(dmpE)
DRG: H9K76_11645(dmpE)
SIMP: C6571_13615(dmpE)
MELA: C6568_07860(dmpE)
MJE: LVC68_01005(dmpE)
STHM: IS481_10530(dmpE)
METP: C1M51_06460(dmpE) C1M51_15400(dmpE)
JAG: GJA_236(xylJ)
JAJ: EKL02_01815(dmpE)
LCH: Lcho_3343
IDC: LRM40_19570(dmpE)
ABRE: pbN1_20560(mhpD)
DEY: HYN24_14130(dmpE) HYN24_14215(dmpE)
FLE: KI610_08575(dmpE) KI610_13420(dmpE) KI610_15465(dmpE)
AZO: azo1853(lapE) azo2434(nahL)
AZR: CJ010_12250(dmpE) CJ010_15220(dmpE)
THK: CCZ27_10770(dmpE) CCZ27_16275(dmpE)
AZQ: G3580_00535(dmpE) G3580_08270(dmpE)
ACIB: ACBT_0261(dmpE)
AFC: AFAEC_1846(dmpE)
AAQI: AAQM_0980(dmpE)
ACLO: ACLO_a0141(dmpE)
ADZ: ADFLV_2553(dmpE)
ALK: ALEK_0381(dmpE)
AANA: AANAER_1258(dmpE)
ARC: ABLL_2444
NAR: Saro_3689
SGI: SGRAN_1581(mhpD)
SPHM: G432_01985
SSAN: NX02_12710
SPKC: KC8_03560
SJP: SJA_C1-11870(mhpD)
SINB: SIDU_08330
SPHB: EP837_03394(bphH)
SFLA: SPHFLASMR4Y_02368(tesE)
HTL: HPTL_0050
CWO: Cwoe_3997
AMOB: HG15A2_23830(tesE)
AG: BAC67694(tesE)
 » show all
Reference
  Authors
Horinouchi M, Hayashi T, Koshino H, Kurita T, Kudo T
  Title
Identification of 9,17-dioxo-1,2,3,4,10,19-hexanorandrostan-5-oic acid, 4-hydroxy-2-oxohexanoic acid, and 2-hydroxyhexa-2,4-dienoic acid and related enzymes involved in testosterone degradation in Comamonas testosteroni TA441.
  Journal
Appl Environ Microbiol 71:5275-81 (2005)
DOI:10.1128/AEM.71.9.5275-5281.2005
  Sequence
LinkDB

KEGG   ENZYME: 4.2.1.132
Entry
EC 4.2.1.132                Enzyme                                 
Name
2-hydroxyhexa-2,4-dienoate hydratase;
tesE (gene name);
hsaE (gene name)
Class
Lyases;
Carbon-oxygen lyases;
Hydro-lyases
Sysname
4-hydroxy-2-oxohexanoate hydro-lyase [(2Z,4Z)-2-hydroxyhexa-2,4-dienoate-forming]
Reaction(IUBMB)
4-hydroxy-2-oxohexanoate = (2Z,4Z)-2-hydroxyhexa-2,4-dienoate + H2O [RN:R09960]
Reaction(KEGG)
R09960;
(other) R05864
Substrate
4-hydroxy-2-oxohexanoate [CPD:C06762]
Product
(2Z,4Z)-2-hydroxyhexa-2,4-dienoate [CPD:C20240];
H2O [CPD:C00001]
Comment
This enzyme catalyses a late step in the bacterial steroid degradation pathway. The product, 4-hydroxy-2-oxohexanoate, forms a 2-hydroxy-4-hex-2-enolactone under acidic conditions.
History
EC 4.2.1.132 created 2012
Pathway
ec00622  Xylene degradation
ec01100  Metabolic pathways
ec01120  Microbial metabolism in diverse environments
Orthology
K18364  2-oxopent-4-enoate/cis-2-oxohex-4-enoate hydratase
Genes
PGELG71_12240
PLUMA4R40_11035
PAKHB0X70_11350
PLUICE143_11265
PSDDSC_09160 DSC_09365
RGLCS053_01760
RTHLRK53_10275(dmpE)
RBDALSL_1080
VAFD1115_22450
PREPCA10_13820 PCA10_p0350(carD)
PPSEBN5_3620(nahL)
PFUWKF707C_12170 KF707C_15560(bzaF) KF707C_16070(salF) KF707C_16250(bphX1)
PCQPcP3B5_25700(tesE_1)
PPIND086(nahL)
PPUTL483_14065
PPUNPP4_27260
PPJRK21_04237
PVRPverR02_11595 PverR02_14690
PSCA458_06520
PSTUUIB01_22740
PBMCL52_14710
PPUUPputUW4_01660(mhpD)
PKCPKB_4239(xylJ)
PFZAV641_10535
PLQAA042_19765
PALKPSAKL28_05940
PPSYAOC04_07835
PFKPFAS1_28775
PSETTHL1_1433
PSILPMA3_11735 PMA3_18070
PKEDLD99_14750
PALLUYA_06720 UYA_06980 UYA_07060 UYA_20155
PUMHGP31_20760
PPSHG5J76_13490
PLALFXN65_06420
PAZEKSS91_19295
PALVKSS97_16725
PPHABVH74_06155 BVH74_16350 BVH74_17960
PZAHU749_013350
PTAENCTC10697_02057(xylJ)
AVNAvin_08670(xylJ) Avin_30610(lapE)
AVLAvCA_08670(xylJ) AvCA_30610(lapE)
AVDAvCA6_08670(xylJ) AvCA6_30610(lapE)
ACXAchr_32780
HPEGEAO82_00555(dmpE) EAO82_09425(dmpE)
HAESLO767_17670(dmpE)
MADHP15_1113 HP15_4034
MSRAU15_21080
MLQASQ50_09215
MSQBKP64_13850
MARJMARI_05440(xylJ)
SPLSpea_2118
SHLShal_2090
SWDSwoo_1392
COLWA3Q33_18575
CBERB5D82_11270
THTE2K93_11200
PHAPSHAa2141(xylJ)
PTNPTRA_a2594(bphH)
PNGPNIG_a2788(bphH)
SPOIIMCC21906_00389 IMCC21906_02710
SNANI6N98_10800
ZALAZF00_03890 AZF00_11430 AZF00_17820
HJABST95_09505
HALCEY643_13295
AFUSEYZ66_02605(dmpE) EYZ66_12965
CYQQ91_0508
CZACYCME_2103
CYYCPC19_10515(dmpE)
BLEPAL038_01300(dmpE)
THISHZT40_10605
AEHMlg_2460
APACS7S_06990
KAKKalk_07855
MGEOCFI10_00425(dmpE) CFI10_04960(dmpE)
MSECLN244_04775 LN244_11330(dmpE)
NCUF0U83_03045(dmpE)
NJPNEJAP_0671(mhpD)
NIKF5I99_10105(dmpE) F5I99_10520(dmpE)
AJPAMJAP_2999(mhpD)
ASIMFE240_03500(dmpE)
ZDFAN401_14585
SOKD0B54_06200
SINIGT972_13730(dmpE) GT972_14670
FCEJN531_005830
GBIPG2T_02230
TBNTBH_C0928
GPBHDN1F_27090(tesE)
NZOSAMEA4504057_0450(xylJ)
NCINCTC10296_00911(xylJ_1)
NWDH3L96_04745(dmpE)
NDULVJ88_00610(dmpE)
VFFVITFI_CDS1370
PTESJQU52_04880(dmpE)
USULVJ78_03680(dmpE)
CHROCXB49_03840
AQLBXU06_16875
RPIRpic_4620
RPFRpic12D_3544
RPJN234_08150 N234_12215
RINACS15_4354(dmpE)
REHH16_B0548(mhpD1) H16_B0597(bphH)
CNCCNE_2c04940(mhpD) CNE_2c05420(xylJ2) CNE_BB1p11990(xylJ1) CNE_BB1p13280(xylJ2)
CUHBJN34_30960
REUReut_A1510 Reut_B5691
RMERmet_1321(xylJ)
CTIRALTA_B0401(mhpD)
CBWRR42_s2313 RR42_s2484
CCUPBKK81_19910 BKK81_27090
CUPBKK80_21900 BKK80_29230
CUUBKK79_35180
COXE0W60_07775(dmpE) E0W60_08025(dmpE)
BVIBcep1808_7603
BURBcep18194_B1459 Bcep18194_B2961
BCJBCAM1613
BCENDM39_3383(dmpE) DM39_4834(dmpE)
BCEOI35_5473
BCTGEM_4111 GEM_5403
BCEDDM42_4993(dmpE) DM42_6345(dmpE)
BCEPAPZ15_31155
BPYRABD05_27820
BCONNL30_04140 NL30_24600
BDFWI26_15985 WI26_21145
BTEIWS51_00560 WS51_05695
BPSLWS57_06045 WS57_12165
BMECWJ16_26790
BSTLBBJ41_10510 BBJ41_21340
BANNJFN94_19125(dmpE) JFN94_25650(dmpE)
BUQAC233_11195
BURKDM992_32625(dmpE)
BXEBxe_C1189(bphH)
BXBDR64_8616(dmpE)
BUZAYM40_21220
BFNOI25_4589(dmpE)
PARBCJU94_37870(dmpE) CJU94_38115(dmpE)
PCAFDSC91_003291
PMEGFNZ07_21165(dmpE)
PPKU875_01900
PRBX636_07570
PVEUC34_07065
CABASBC2_49020(tesE)
LIMNHKT17_03820(dmpE)
BOJCBF45_01490 CBF45_04500 CBF45_06955
CDNBN940_01166 BN940_04316
KGYEHF36_01675(dmpE) EHF36_04885(dmpE)
PNAPnap_4144
AJSAjs_0222
ACIDCBP33_01105
ACIPCBP36_01200
ACINCBP34_01110
ACISCBP35_17750
AMONH9L24_14185(dmpE)
VEIVeis_2785
VBOCKY39_28505(dmpE)
CTTCtCNB1_1353 CtCNB1_3149
CTESO987_07740
COFFOZ74_08305(dmpE)
ADNAlide_0337
ADKAlide2_0282
HYLLPB072_18180
HPSEHPF_01870(xylJ)
HYNF9K07_02750(dmpE)
HTNKI616_01965(dmpE)
DIHG7047_01670(dmpE) G7047_09705
DRGH9K76_11645(dmpE)
SIMPC6571_13615(dmpE)
MELAC6568_07860(dmpE)
MJELVC68_01005(dmpE)
STHMIS481_10530(dmpE)
MPTMpe_A2273 Mpe_A3325
METPC1M51_06460(dmpE) C1M51_15400(dmpE)
JAGGJA_236(xylJ)
JAJEKL02_01815(dmpE)
MASWAM586_18650
LCHLcho_3343
SNNEWH46_19325
IDCLRM40_19570(dmpE)
RBUPG1C_14105
ABREpbN1_20560(mhpD)
DARDaro_2774 Daro_3785
DEYHYN24_14130(dmpE) HYN24_14215(dmpE)
FLEKI610_08575(dmpE) KI610_13420(dmpE) KI610_15465(dmpE)
AZOazo1853(lapE) azo2434(nahL)
AOAdqs_2006 dqs_2578
AZIAzCIB_1507 AzCIB_4411
AZDCDA09_09955 CDA09_10325(dmpE) CDA09_10440(dmpE) CDA09_21440(dmpE)
AZRCJ010_12250(dmpE) CJ010_15220(dmpE)
TMZTmz1t_2077 Tmz1t_3091
THUAC731_001810 AC731_008895
THKCCZ27_10770(dmpE) CCZ27_16275(dmpE)
AZQG3580_00535(dmpE) G3580_08270(dmpE)
ACIBACBT_0261(dmpE)
AFCAFAEC_1846(dmpE)
AAQIAAQM_0980(dmpE)
ACLOACLO_a0141(dmpE)
ADZADFLV_2553(dmpE)
ALKALEK_0381(dmpE)
AANAAANAER_1258(dmpE)
ARCABLL_2444
NTDEGO55_13680
TECAKL02_008255
NARSaro_3689
NPPPP1Y_AT32745 PP1Y_Mpl3103
NPNJI59_22525
NOVTQ38_027295
NOTC7W88_20925
SGISGRAN_1581(mhpD)
SPHMG432_01985
SSANNX02_12710
SPKCKC8_03560
SPHAD3Y57_15215
SLANGV829_01855
SJPSJA_C1-11870(mhpD)
SINBSIDU_08330
SYBTZ53_09010 TZ53_23455
SPHBEP837_03394(bphH)
SHYDCJD35_06490
SUFLFIL70_19005
SBARH5V43_20655
SFLASPHFLASMR4Y_02368(tesE)
SPHJBSL82_10455
PALGHFP57_16665
PHAOHF685_05520
TFVIDJ81_04970
PHZCHX26_05320
PEGE5R92_01705
HTLHPTL_0050
ASEDIRT44_17630
MHAPJFY71_09925
CWOCwoe_3997
AMOBHG15A2_23830(tesE)
NAYHYG81_22605
 » show all
Reference
1  [PMID:16151114]
  Authors
Horinouchi M, Hayashi T, Koshino H, Kurita T, Kudo T
  Title
Identification of 9,17-dioxo-1,2,3,4,10,19-hexanorandrostan-5-oic acid, 4-hydroxy-2-oxohexanoic acid, and 2-hydroxyhexa-2,4-dienoic acid and related enzymes involved in testosterone degradation in Comamonas testosteroni TA441.
  Journal
Appl Environ Microbiol 71:5275-81 (2005)
DOI:10.1128/AEM.71.9.5275-5281.2005
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 4.2.1.132
IUBMB Enzyme Nomenclature: 4.2.1.132
ExPASy - ENZYME nomenclature database: 4.2.1.132
UM-BBD (Biocatalysis/Biodegradation Database): 4.2.1.132
BRENDA, the Enzyme Database: 4.2.1.132
LinkDB

KEGG   REACTION: R05864
Entry
R05864                      Reaction                               
Name
4-Hydroxy-2-oxohexanoate hydro-lyase
Definition
2-Hydroxy-cis-hex-2,4-dienoate + H2O <=> 4-Hydroxy-2-oxohexanoic acid
Equation
Reaction class
RC02676  C06762_C11354
Enzyme
Pathway
rn00622  Xylene degradation
rn01100  Metabolic pathways
rn01120  Microbial metabolism in diverse environments
rn01220  Degradation of aromatic compounds
Module
M00569  Catechol meta-cleavage, catechol => acetyl-CoA / 4-methylcatechol => propanoyl-CoA
Orthology
K18364  2-oxopent-4-enoate/cis-2-oxohex-4-enoate hydratase [EC:4.2.1.80 4.2.1.132]
LinkDB

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