KEGG   ORTHOLOGY: K20519
Entry
K20519            Tight     KO                                     
Symbol
SS
Name
sclareol synthase [EC:4.2.3.141]
Pathway
map00904  Diterpenoid biosynthesis
map01110  Biosynthesis of secondary metabolites
Reaction
R10241  (13E)-8alpha-hydroxylabd-13-en-15-yl-diphosphate diphosphate-lyase (sclareol forming)
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09109 Metabolism of terpenoids and polyketides
   00904 Diterpenoid biosynthesis
    K20519  SS; sclareol synthase
Enzymes [BR:ko01000]
 4. Lyases
  4.2  Carbon-oxygen lyases
   4.2.3  Acting on phosphates
    4.2.3.141  sclareol synthase
     K20519  SS; sclareol synthase
Genes
AG: AFU61898(SS)
Reference
PMID:22834731
  Authors
Caniard A, Zerbe P, Legrand S, Cohade A, Valot N, Magnard JL, Bohlmann J, Legendre L
  Title
Discovery and functional characterization of two diterpene synthases for sclareol biosynthesis in Salvia sclarea (L.) and their relevance for perfume manufacture.
  Journal
BMC Plant Biol 12:119 (2012)
DOI:10.1186/1471-2229-12-119
  Sequence
[ag:AFU61898]
LinkDB

KEGG   ENZYME: 4.2.3.141
Entry
EC 4.2.3.141                Enzyme                                 
Name
sclareol synthase;
SS
Class
Lyases;
Carbon-oxygen lyases;
Acting on phosphates
Sysname
(13E)-8alpha-hydroxylabd-13-en-15-yl-diphosphate-lyase (sclareol-forming)
Reaction(IUBMB)
(13E)-8alpha-hydroxylabd-13-en-15-yl diphosphate + H2O = sclareol + diphosphate [RN:R10241]
Reaction(KEGG)
R10241
Substrate
(13E)-8alpha-hydroxylabd-13-en-15-yl diphosphate [CPD:C20270];
H2O [CPD:C00001]
Product
sclareol [CPD:C09183];
diphosphate [CPD:C00013]
Comment
Isolated from the plant Salvia sclarea (clary sage). Originally thought to be synthesized in one step from geranylgeranyl diphosphate it is now known to require two enzymes, EC 4.2.1.133, copal-8-ol diphosphate synthase and EC 4.2.3.141, sclareol synthase. Sclareol is used in perfumery.
History
EC 4.2.3.141 created 2013, modified 2017
Pathway
ec00904  Diterpenoid biosynthesis
ec01110  Biosynthesis of secondary metabolites
Orthology
K20519  sclareol synthase
Reference
1  [PMID:22834731]
  Authors
Caniard A, Zerbe P, Legrand S, Cohade A, Valot N, Magnard JL, Bohlmann J, Legendre L
  Title
Discovery and functional characterization of two diterpene synthases for sclareol biosynthesis in Salvia sclarea (L.) and their relevance for perfume manufacture.
  Journal
BMC Plant Biol 12:119 (2012)
DOI:10.1186/1471-2229-12-119
  Sequence
[ag:AFU61898]
Other DBs
ExplorEnz - The Enzyme Database: 4.2.3.141
IUBMB Enzyme Nomenclature: 4.2.3.141
ExPASy - ENZYME nomenclature database: 4.2.3.141
BRENDA, the Enzyme Database: 4.2.3.141
LinkDB

KEGG   REACTION: R10241
Entry
R10241                      Reaction                               
Name
(13E)-8alpha-hydroxylabd-13-en-15-yl-diphosphate diphosphate-lyase (sclareol forming);
copal-8-ol-diphosphate diphosphate-lyase (sclareol-forming)
Definition
Copal-8-ol diphosphate + H2O <=> Sclareol + Diphosphate
Equation
C20270 + C00001 <=> C09183 + C00013
Reaction class
RC01821  C09183_C20270
Enzyme
4.2.3.141
Pathway
rn00904  Diterpenoid biosynthesis
rn01110  Biosynthesis of secondary metabolites
Brite
Enzymatic reactions [BR:br08201]
 4. Lyase reactions
  4.2  Carbon-oxygen lyases
   4.2.3  Acting on phosphates
    4.2.3.141
     R10241  Copal-8-ol diphosphate + H2O <=> Sclareol + Diphosphate
Orthology
K20519  sclareol synthase [EC:4.2.3.141]
Other DBs
RHEA: 34462
LinkDB

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