KEGG   PATHWAY: bfai03030
Entry
bfai03030                   Pathway                                
Name
DNA replication - Candidatus Borrelia fainii
Description
A complex network of interacting proteins and enzymes is required for DNA replication. Generally, DNA replication follows a multistep enzymatic pathway. At the DNA replication fork, a DNA helicase (DnaB or MCM complex) precedes the DNA synthetic machinery and unwinds the duplex parental DNA in cooperation with the SSB or RPA. On the leading strand, replication occurs continuously in a 5 to 3 direction, whereas on the lagging strand, DNA replication occurs discontinuously by synthesis and joining of short Okazaki fragments. In prokaryotes, the leading strand replication apparatus consists of a DNA polymerase (pol III core), a sliding clamp (beta), and a clamp loader (gamma delta complex). The DNA primase (DnaG) is needed to form RNA primers. Normally, during replication of the lagging-strand DNA template, an RNA primer is removed either by an RNase H or by the 5 to 3 exonuclease activity of DNA pol I, and the DNA ligase joins the Okazaki fragments. In eukaryotes, three DNA polymerases (alpha, delta, and epsilon) have been identified. DNA primase forms a permanent complex with DNA polymerase alpha. PCNA and RFC function as a clamp and a clamp loader. FEN 1 and RNase H1 remove the RNA from the Okazaki fragments and DNA ligase I joins the DNA.
Class
Genetic Information Processing; Replication and repair
Pathway map
bfai03030  DNA replication
bfai03030

Organism
Candidatus Borrelia fainii [GN:bfai]
Gene
BOFE_02900  dnaE; DNA polymerase III subunit alpha [KO:K02337] [EC:2.7.7.7]
BOFE_01100  hypothetical protein [KO:K02343] [EC:2.7.7.7]
BOFE_04010  DNA polymerase III subunit gamma/tau [KO:K02343] [EC:2.7.7.7]
BOFE_04060  DNA polymerase III subunit delta [KO:K02340] [EC:2.7.7.7]
BOFE_04280  dnaN; DNA polymerase III subunit beta [KO:K02338] [EC:2.7.7.7]
BOFE_01060  hypothetical protein [KO:K02314] [EC:5.6.2.3]
BOFE_07450  dnaB; replicative DNA helicase [KO:K02314] [EC:5.6.2.3]
BOFE_01630  dnaG; DNA primase [KO:K02316] [EC:2.7.7.101]
BOFE_07420  ssb; single-stranded DNA-binding protein [KO:K03111]
BOFE_08050  rnhB; ribonuclease HII [KO:K03470] [EC:3.1.26.4]
BOFE_03180  polA; DNA polymerase I [KO:K02335] [EC:2.7.7.7]
BOFE_03140  ligA; DNA ligase [KO:K01972] [EC:6.5.1.2]
Reference
PMID:8087839
  Authors
Stillman B.
  Title
Smart machines at the DNA replication fork.
  Journal
Cell 78:725-8 (1994)
DOI:10.1016/S0092-8674(94)90362-X
Reference
PMID:9759502
  Authors
Waga S, Stillman B.
  Title
The DNA replication fork in eukaryotic cells.
  Journal
Annu Rev Biochem 67:721-51 (1998)
DOI:10.1146/annurev.biochem.67.1.721
KO pathway
ko03030   
LinkDB

DBGET integrated database retrieval system