Entry
Name
3beta-hydroxy-Delta5-steroid dehydrogenase;
progesterone reductase;
Delta5-3beta-hydroxysteroid dehydrogenase;
3beta-hydroxy-5-ene steroid dehydrogenase;
3beta-hydroxy steroid dehydrogenase/isomerase;
3beta-hydroxy-Delta5-C27-steroid dehydrogenase/isomerase;
3beta-hydroxy-Delta5-C27-steroid oxidoreductase;
3beta-hydroxy-5-ene-steroid oxidoreductase;
steroid-Delta5-3beta-ol dehydrogenase;
3beta-HSDH;
5-ene-3-beta-hydroxysteroid dehydrogenase;
3beta-hydroxy-5-ene-steroid dehydrogenase
Class
Oxidoreductases;
Acting on the CH-OH group of donors;
With NAD+ or NADP+ as acceptor
BRITE hierarchy
Sysname
3beta-hydroxy-Delta5-steroid:NAD+ 3-oxidoreductase
Reaction(IUBMB)
a 3beta-hydroxy-Delta5-steroid + NAD+ = a 3-oxo-Delta5-steroid + NADH + H+ [RN:
R03176 ]
Reaction(KEGG)
Substrate
3beta-hydroxy-Delta5-steroid [CPD:
C03836 ];
NAD+ [CPD:
C00003 ]
Product
Comment
This activity is found in several bifunctional enzymes that catalyse the oxidative conversion of Delta5-3-hydroxy steroids to a Delta4-3-oxo configuration. This conversion is carried out in two separate, sequential reactions; in the first reaction, which requires NAD+, the enzyme catalyses the dehydrogenation of the 3beta-hydroxy steroid to a 3-oxo intermediate. In the second reaction the reduced cosubstrate, which remains attached to the enzyme, activates the isomerization of the Delta5 form to a Delta4 form (cf. EC
5.3.3.1 , steroid Delta-isomerase). Substrates include dehydroepiandrosterone (which is converted into androst-5-ene-3,17-dione), pregnenolone (converted to progesterone) and cholest-5-en-3-one, an intermediate of cholesterol degradation.
History
EC 1.1.1.145 created 1972
Pathway
ec00140 Steroid hormone biosynthesis
ec01120 Microbial metabolism in diverse environments
Orthology
K00070 3beta-hydroxy-Delta5-steroid dehydrogenase / steroid Delta-isomerase
K16045 3beta-hydroxy-Delta5-steroid dehydrogenase / steroid Delta-isomerase
K26407 Poxviridae/Iridoviridae 3beta-hydroxy-Delta5-steroid dehydrogenase / steroid Delta-isomerase
Genes
HSA : 3283(HSD3B1) 3284(HSD3B2)
PTR : 457165(HSD3B2) 469446
PPS : 100982464(HSD3B2) 100983009 100990187
GGO : 101141070 101141801 101146563(HSD3B2)
PON : 100451655 100452394 100455460
PPYG : 129015890 129015909 129015918
NLE : 100596087 100605995(HSD3B2) 100606563 100607955
HMH : 116469522 116469530(HSD3B2)
SSYN : 134733865 134733875 134734675(HSD3B1)
MCC : 712686(HSD3B2) 713091 713272 715337
MCF : 102133592 102134747 102135110
MTHB : 126931035 126931104 126931154 126951788
MNI : 105479071 105479074 105479078 105479082 105479084(HSD3B2)
CSAB : 103224104 103224109(HSD3B2)
CATY : 105599590 105599597(HSD3B2)
PANU : 100999145 101018740 101019096(HSD3B2)
TGE : 112624233 112634262(HSD3B2)
MLEU : 105541296(HSD3B2) 105541330
RRO : 104654599 104654602 104654605
RBB : 108528160 108528161 108528164
PTEH : 111549589 111549591
CANG : 105505264 105505267
CJC : 100387484 100389654 100400416 100405879
CIMI : 108297130 108301104
ANAN : 105721680(HSD3B2) 105721706 110567017
MMU : 100043456(Hsd3b8) 100043461(Hsd3b9) 15492(Hsd3b1) 15493(Hsd3b2) 15494(Hsd3b3) 15495(Hsd3b4) 15496(Hsd3b5) 15497(Hsd3b6)
MCAL : 110288898 110290813 110290978 110291292 110291371 110291888
MPAH : 110315378 110319834 110319836 110320478 110320490
RNO : 24470(Hsd3b5) 29632(Hsd3b2) 360348(Hsd3b1) 682974(Hsd3b3)
MCOC : 116093252 116094199 116094200 116094201
ANU : 117707219 117707246 117707247 117707273 117707523
MUN : 110542496 110543775 110544885 110544899 110563733 110563735 132656956 132656957 132656958 132656961 132656962 132656967 132657118
CGE : 100770922 113830678 113833179 113833198
MAUA : 101837099 101842851(Hao2) 121138738 121142382
PROB : 127232674 127232675
PLEU : 114688199 114688619 114688620 114688621 114688750 114688751 114688752 114698547
MORG : 121437803 121437911 121438015 121438017
MFOT : 126490105 126510847
AAMP : 119801286 119801287 119801288 119801289
NGI : 103728305 103732007 103738837
HGL : 101707488(Hsd3b1) 101707858
CPOC : 100379573(Hsd3b1) 100725531 100725801
CCAN : 109702728 109702729
DORD : 105991916 105991917
PLOP : 125344738 125345248 125345249 125345254 125345257 125345259 125345261 125355478 125355479 125355483
NCAR : 124964015 124990189 124992819
MMMA : 107156205(Hao2) 107156207 107156209
SARA : 101555226 101555490
TPAI : 128076148 128090414
DRE : 373131(hsd3b2) 553532(hsd3b1)
CAUA : 113108554 113108799
CGIB : 127965123 128019700
CHAR : 105913236 105913280 105913281
PLEP : 121949401(hsd3b1) 121963657
MSAM : 119884914 119904488(hsd3b1)
HHIP : 117755029 117755030
AANG : 118214002 118214003
ARUT : 117406697 117406856
CMK : 103180179(hsd3b1) 121852133
BBEL : 109470289 109481615
DSI : Dsimw501_GD14693(Dsim_GD14693)
AALB : 109407756 109418935
RSAN : 119377584 119390488
CANU : 128186867 128188117 128188594
CVN : 111136775 111137023 111137166
OED : 125654276 125659193 125659727
PMAX : 117333059 117333060 117333062 117333069
MMER : 123525098 123534278 123534660 123535125 123535257 123535266 123552007
RPHI : 132742228 132742229 132743368 132753356 132759419 132759420
DPOL : 127839711 127856352 127861538
MAEA : 128229618 128232487 128232575
LLON : 135485103 135502377
» show all
Taxonomy
Reference
Authors
Cheatum SG, Watten JC.
Title
Purification and properties of 3-beta-hydroxysteroid dehydrogenase and delta-5-3-ketosteroid isomerase from bovine corpora lutea.
Journal
Reference
Authors
KORITZ SB.
Title
THE CONVERSION OF PREGNENOLONE TO PROGESTERONE BY SMALL PARTICLES FROM RAT ADRENAL.
Journal
Reference
3
Authors
Neville AM, Orr JC, Engel LL.
Title
Delta5-3beta-Hydroxy steroid dehydrogenase activities of bovine adrenal cortex.
Journal
Biochem J 107:20 (1968)
Other DBs
ExPASy - ENZYME nomenclature database: 1.1.1.145
LinkDB
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