KEGG   ENZYME: 3.4.22.56
Entry
EC 3.4.22.56                Enzyme                                 
Name
caspase-3;
CPP32;
apopain;
yama protein
Class
Hydrolases;
Acting on peptide bonds (peptidases);
Cysteine endopeptidases
Reaction(IUBMB)
Strict requirement for an Asp residue at positions P1 and P4. It has a preferred cleavage sequence of Asp-Xaa-Xaa-Asp! with a hydrophobic amino-acid residue at P2 and a hydrophilic amino-acid residue at P3, although Val or Ala are also accepted at this position
Comment
Caspase-3 is an effector/executioner caspase, as are caspase-6 (EC 3.4.22.59) and caspase-7 (EC 3.4.22.60) [5]. These caspases are responsible for the proteolysis of the majority of cellular polypeptides [e.g. poly(ADP-ribose) polymerase (PARP)], which leads to the apoptotic phenotype [3,5]. Procaspase-3 can be activated by caspase-1 (EC 3.4.22.36), caspase-8 (EC 3.4.22.61), caspase-9 (EC 3.4.22.62) and caspase-10 (EC 3.4.22.63) as well as by the serine protease granzyme B [1]. Caspase-3 can activate procaspase-2 (EC 3.4.22.55) [2]. Activation occurs by inter-domain cleavage followed by removal of the N-terminal prodomain [6]. Although Asp-Glu-(Val/Ile)-Asp is thought to be the preferred cleavage sequence, the enzyme can accommodate different residues at P2 and P3 of the substrate [4]. Like caspase-2, a hydrophobic residue at P5 of caspase-3 leads to more efficient hydrolysis, e.g. (Val/Leu)-Asp-Val-Ala-Asp! is a better substrate than Asp-Val-Ala-Asp! . This is not the case for caspase-7 [4]. Belongs in peptidase family C14.
History
EC 3.4.22.56 created 2007
Orthology
K02187  caspase 3
Genes
HSA836(CASP3)
PTR461669(CASP3)
PPS100974934(CASP3)
GGO101126742(CASP3)
PON100435491(CASP3)
NLE100585064(CASP3)
HMH116466067(CASP3)
MCC694473(CASP3)
MCF102144182(CASP3)
MTHB126954341
MNI105466747(CASP3) 105492342
CSAB103236614(CASP3)
CATY105593734 105597738(CASP3)
PANU101023728(CASP3)
TGE112622580 112625066(CASP3)
MLEU105552669 105553959(CASP3)
RRO104666783 104673791(CASP3)
RBB108512689
TFN117075139 117083313 117086196(CASP3)
PTEH111548479(CASP3) 111551382
CANG105524344(CASP3)
CJC100387570(CASP3)
SBQ101029697(CASP3)
CIMI108288114(CASP3) 108310786
CSYR103257846
MMUR105880624 105882502(CASP3)
LCAT123638644(CASP3)
PCOQ105810111(CASP3)
OGA100961435(CASP3)
MMU12367(Casp3)
MCAL110299851(Casp3)
MPAH110336608(Casp3)
RNO25402(Casp3)
MCOC116069240(Casp3)
ANU117721984(Casp3)
MUN132653623(Casp3)
CGE100689061(Casp3)
MAUA101828960(Casp3)
PROB127220536(Casp3)
PLEU114693817(Casp3)
MORG121433874(Casp3)
MFOT126495899 126504830
AAMP119812861(Casp3)
NGI103744668 103750475(Casp3)
HGL101696390 101712867(Casp3) 101723884
CPOC100718309(Casp3)
CCAN109678444 109678454
DORD105992928(Casp3)
DSP122126104(Casp3)
PLOP125339355(Casp3)
NCAR124972492 124983898
MMMA107142678(Casp3) 107158031
OCU100008840(CASP3)
OPI101531640(CASP3)
TUP102472926(CASP3) 102487119
GVR103589530(CASP3)
CFA403567(CASP3)
CLUD112675231(CASP3)
VVP112934766(CASP3)
VLG121489913(CASP3)
NPO129503548(CASP3)
AML100469056(CASP3)
UMR103680991(CASP3)
UAH113262259(CASP3)
UAR123784520(CASP3)
ELK111144607
LLV125091274
MPUF101690858(CASP3)
MNP132006389(CASP3)
MLK131820467(CASP3)
NVS122889990(CASP3)
ORO101371336(CASP3)
EJU114206181(CASP3)
ZCA113924509(CASP3)
MLX118010074(CASP3)
NSU110584089(CASP3)
LWW102735000(CASP3)
FCA493932(CASP3)
PYU121039672(CASP3)
PCOO112870035(CASP3)
PBG122470647(CASP3)
PVIV125163566(CASP3)
LRUF124520277
PTG102954729(CASP3)
PPAD109251018(CASP3)
PUC125923904
AJU106979326
HHV120246375(CASP3)
BTA408016(CASP3)
BOM102287348(CASP3)
BIU109553316(CASP3)
BBUB102415768(CASP3)
BBIS104985538(CASP3)
CHX102177031(CASP3)
OAS443031(CASP3)
BTAX128068336(CASP3)
ODA120865721(CASP3)
CCAD122432443(CASP3)
MBEZ129549305(CASP3)
SSC397244(CASP3)
CFR102520133(CASP3)
CBAI105067685(CASP3)
CDK105084433(CASP3)
VPC102541258(CASP3)
BACU103019939(CASP3)
BMUS118887867(CASP3) 118889206
LVE103073819(CASP3)
OOR101289847(CASP3)
DLE111173401(CASP3)
PCAD102977692(CASP3)
PSIU116746660(CASP3)
NASI112409662(CASP3)
ECB100034083(CASP3)
EPZ103549132(CASP3)
EAI106830081(CASP3)
MYB102258599(CASP3)
MYD102760961(CASP3)
MMYO118650210(CASP3)
MLF102438419(CASP3)
PKL118729116(CASP3)
EFUS103295435(CASP3)
MNA107535740(CASP3)
DRO112306749(CASP3)
SHON118993073(CASP3)
AJM119036917(CASP3)
PDIC114508735(CASP3)
PHAS123807416(CASP3)
MMF118622705(CASP3)
PPAM129064891(CASP3)
HAI109395706(CASP3)
RFQ117021507(CASP3)
PALE102897289(CASP3)
PGIG120618435(CASP3)
PVP105294710(CASP3)
RAY107510456(CASP3)
MJV108389793(CASP3)
TOD119257947(CASP3)
SARA101539340(CASP3)
LAV100672256(CASP3)
TMU101354307
ETF101643856(CASP3)
DNM101441936(CASP3)
MDO654273(CASP3) 654275
GAS123232391 123251682(CASP3) 123251883 123252074 123255551
SHR100922500(CASP3) 100922757
AFZ127542048
PCW110222594(CASP3)
OAA100087105(CASP3)
GGA395476(CASP3)
PCOC116238124(CASP3)
MGP100548182(CASP3)
CJO107313280(CASP3)
TPAI128080681(CASP3)
LMUT125692760(CASP3)
NMEL110397291(CASP3)
APLA101805296(CASP3)
ACYG106035172(CASP3)
CATA118253463(CASP3)
AFUL116489142(CASP3)
TGU100230799(CASP3)
LSR110477516(CASP3)
SCAN103825669(CASP3)
PMOA120497893(CASP3)
OTC121348441(CASP3)
PRUF121360209(CASP3)
GFR102035830(CASP3)
FAB101808212(CASP3)
OMA130252292(CASP3)
PHI102104322(CASP3)
PMAJ107203069(CASP3)
CCAE111945572(CASP3)
CCW104690575(CASP3)
CBRC103622495(CASP3)
ETL114055579(CASP3)
ZAB102061136(CASP3)
ACHL103797011(CASP3)
SVG106861352(CASP3)
MMEA130575942(CASP3)
HRT120753533(CASP3)
FPG101913355(CASP3)
FCH102056397(CASP3)
CLV102089427(CASP3)
EGZ104129473(CASP3)
NNI104021286(CASP3)
PCRI104030021(CASP3)
PLET104617747(CASP3)
EHS104506672(CASP3)
PCAO104053240(CASP3)
ACUN113479376(CASP3)
TALA104365328(CASP3)
PADL103922530(CASP3)
AFOR103897361(CASP3)
ACHC115337302(CASP3)
HALD104321147(CASP3)
HLE104833382(CASP3)
AGEN126036926
GCL127015618
CCRI104160818(CASP3)
CSTI104549480
CMAC104485519(CASP3)
MUI104540444(CASP3)
BREG104641770(CASP3)
FGA104072785(CASP3)
GSTE104257206(CASP3)
LDI104353695(CASP3)
MNB103778241(CASP3)
OHA104328901(CASP3)
NNT104403122(CASP3)
SHAB115610967(CASP3)
DPUB104309548(CASP3)
PGUU104466977(CASP3)
ACAR104529212(CASP3)
CPEA104393005(CASP3)
AVIT104271283
CVF104292396(CASP3)
RTD128909768(CASP3)
CUCA104063486(CASP3)
TEO104382450(CASP3)
BRHI104488987(CASP3)
AAM106484082(CASP3)
AROW112974750(CASP3)
NPD112942609(CASP3)
TGT104568965
DNE112986139(CASP3)
SCAM104145229(CASP3)
ASN102383553(CASP3)
AMJ102572621(CASP3)
CPOO109309794(CASP3)
GGN109301036(CASP3)
PSS102463409
CMY102942062(CASP3)
CCAY125635802(CASP3)
DCC119854273(CASP3)
CPIC101948246(CASP3)
TST117877835(CASP3)
CABI116836650(CASP3)
MRV120406161(CASP3)
ACS100563931(casp3)
ASAO132776702(CASP3)
PVT110083865(CASP3)
SUND121930895(CASP3)
PBI103049711(CASP3)
PMUR107302670(CASP3)
CTIG120302417(CASP3)
TSR106539846(CASP3)
PGUT117654566(CASP3)
APRI131202823(CASP3)
PTEX113434641(CASP3)
NSS113411860(CASP3)
VKO123033290(CASP3)
PMUA114585959 114604250(CASP3)
PRAF128420708(CASP3)
ZVI118083396(CASP3)
HCG128326507(CASP3)
GJA107115654(CASP3)
STOW125439786(CASP3)
EMC129337174(CASP3)
XLA100036910(casp3.2.L) 397720(casp3.2.S)
XTR100151728(casp3.2) 100486222(casp3.1)
NPR108794589 108794597
RTEM120921481(CASP3) 120921507
BBUF120990143 120990145
BGAR122934399 122940788
MUO115461581(CASP3)
GSH117359902(CASP3)
DRE140621(casp3a) 566604(casp3b)
SRX107710482(casp3) 107716035 107716036 107746030 107754122
SANH107656460 107678640 107688004(casp3) 107702336
SGH107556843 107558201(casp3) 107562747 107589121
CCAR109068105 109068106 109071297 109089113 109093929 109096565
CAUA113044167 113074954 113074955 113105596 113113788 113113799
CGIB127949347 127966708 127970959 128027693
PTET122347890(casp3a) 122358134(casp3b)
LROH127164613(casp3a) 127176487(casp3b) 127176488
OMC131537993(casp3a) 131552912(casp3b)
PPRM120462979 120465362(casp3b) 120488777(casp3a)
RKG130078661(casp3b) 130089485(casp3a)
MAMB125266884(casp3b) 125272552(casp3a)
CIDE127494251 127517789
MASI127413339 127418172 127444133 127444697
TROS130552814(casp3a) 130563511(casp3b)
TDW130438496(casp3b) 130438623(casp3a)
MANU129422652 129422653 129444794(casp3a)
IPU100528811(casp3b) 108260860(casp3a)
IFU128604109(casp3a) 128611217(casp3b)
PHYP113529132 113547376
SMEO124381088(casp3a) 124386509(casp3b)
TFD113635419(casp3a) 113635854(casp3b)
TVC132841980(casp3a) 132855819(casp3b)
AMEX103025383 111189829
CMAO118806926 118806927 118817001
EEE113581517(casp3) 113586537
CHAR105892611(casp3a) 105894697
TRU446083(casp3)
TFS130527988(casp3a)
TNGGSTEN00013715G001
LCO104928227 104936945(caspase-3)
NCC104959742 104967108(casp3)
TBEN117475166 117482205(casp3a)
CGOB115010976(casp3) 115014922
PGEO117440899 117448400(casp3a)
GACU117550380 117556108(casp3a)
EMAC134859666 134864823(casp3a)
ELY117254954(casp3a) 117261186(casp3b)
EFO125886058(casp3a) 125894522(casp3b)
PLEP121942065(casp3a) 121948074(casp3b) 121963193
SLUC116034351(casp3a) 116036562 116036563 116036564
ECRA117951380 117960699(casp3a)
ESP116669567 116697073
PFLV114563090 114563091 114570899
GAT120818221 120818253 120824960(casp3a)
PPUG119211291(casp3b) 119217562(casp3a)
AFB129089954 129095256
CLUM117730679(casp3a) 117737645
PSWI130190550(casp3a) 130210015
MSAM119888653 119898808(casp3a)
SCHU122873701(casp3a) 122879777
CUD121506610 121508374(casp3a)
ALAT119007045 119010646(casp3a)
MZE101463771 101464077 101478576
ONL100534438(casp3a) 100695417 100695678
OAU116333961 116334002(casp3a) 120432681
OLA100049215(casp3) 100049265
OML112137236(casp3a) 112153020
CSAI133441481(casp3a)
XMA102224433 102227213
XCO114139499 114144355(casp3)
XHE116714723 116720798
PRET103461041 103471007
PFOR103136385(casp3) 103151710 103151718
PLAI106934607 106964569(casp3)
PMEI106906082 106931037
GAF122830167(casp3a) 122837161
PPRL129362835 129376856(casp3a)
CVG107090480 107094160(casp3)
CTUL119774386(casp3a) 119794097
GMU124860636 124868646(casp3a)
NFU107390752(casp3) 107397381
KMR108234461(casp3a) 108240012
ALIM106525841 106532390 106532391
NWH119408300(casp3b) 119422562(casp3a)
AOCE111577084 111580236 111580983
MCEP125004376(casp3a) 125007842
CSEM103383467 103391460(casp3)
POV109631395 109639599
SSEN122770713(casp3a) 122778867(casp3b)
HHIP117768955 117768956 117770486(casp3a)
HSP118112335 118112812 118125353(casp3a)
SMAU118312563(casp3a) 118319217
LCF108893087 108901489(casp3b)
SDU111216621 111226544
SLAL111663309 111667597
XGL120785392 120800586(casp3a)
HCQ109511417 109515795
SSCV125969100 125977455
SBIA133505663(casp3a) 133509169(casp3b)
PEE133403901(casp3a) 133407620(casp3b)
PTAO133476745(casp3a) 133484344(casp3b)
BPEC110157710 110157737 110157738 110157739 110160793 110167549
MALB109957512(casp3) 109972113
BSPL114858837(casp3a) 114864529(casp3b)
SJO128362971(casp3b) 128369792(casp3a)
SASA100194892(casp3) 106602722(CASP3) 106612018
STRU115164279 115192071 115205518
OTW112231666(casp3a) 112248666 112255992(casp3b) 112261439
OMY100653443(casp3b) 110497496 110525880 110534285(casp3a)
OGO124001817 124010499(casp3b) 124012367(casp3a) 124034151
ONE115111788 115129259 115137223(casp3) 115145810
OKI109882220 109894209 109901037 109905000
OKE118362899 118363298(casp3b) 118393409 118395316(casp3a)
SALP111967296 111974694 112068696
SNH120035095 120040359 120052764(casp3a) 120062476(casp3b)
CCLU121561955(casp3a) 121585038 121586839(casp3b)
ELS105006735(casp3) 105029385(casp3)
SFM108924572(casp3) 114912449
PKI111853593(casp3)
AANG118228056(casp3a) 118231090
LOC102691196
PSPA121297547 121320508
ARUT117409020 117420645 117420663
PSEX120527520
LCM106705928(CASP3)
CMK103187405 103190313
LERI129695247(casp3b)
SCLV120329950 120330440 120343279
SPU100888153 100891400 105440861 115924745 577169 590727 752445
LPIC129262862 129276743 129280887
APLC110975794
SKO102802674 102806314
DPO15382886
DPE113565090
DMN108158700
DHE111592184
RZE108355211
TDA119666956 119674301
SCAC106083170 106085300
LCQ111676405
ECOE129940714
AARA120900924
AMER121599888
AAG5567300
TPRE106649650
PPYR116180848
VCD124531149
PXU106127530
SLIU111352486
CCRN123291062
FOC113216828
DPXDAPPUDRAFT_45861
DMK116936557
PJA122244525 122252835 122266857
PCHN125025456 125027331 125038862 125045343
HAME121858242
PCLA123746535
CQD128692352
PTRU123499673
ESN127004372
HAZT108673778
LSM121116020 121116612
VDE111249074
VJA111272320
DFR124494679
CSCU111622357 111630059
ABRU129981352
SDM118179528 118199480
LPOL106473956
NAINECAME_11879
HROHELRODRAFT_111847
LGILOTGIDRAFT_134425 LOTGIDRAFT_154127
PVUL126810934 126825563
BGT106054357 106055843 106060604 106060606
GAE121371420 121373089 121373751 121379721 121380116 121380117
HRF124110711 124138235 124145235 124149347 124151146
HRJ124271978 124273441 124275872 124277155 124280186 124282813 124284013
CRG105325616 105343829 117684706
CVN111103742 111124840 111124890 111124931 111135767
CANU128157037 128177778
OED125678412
MYI110452312
PMAX117318643 117319579 117324924 117329263 117329267 117337756 117344515
MMER123540593 123564484
RPHI132712765 132712767 132719291 132720393 132744926 132752043 132752568
DPOL127842540 127866357 127875975
OBI106868738
OSN115211714 115211715 115212057 115212312 115213954 115221299 115222278 115226488 115226968 115227436 115227469 118761171
LAK106173180
SMMSmp_028500
OVIT265_01459
EGLEGR_04745
NVE5502486
EPA110247107
ATEN116289657 116305833
ADF107330835 107333405 107341447 107353606 107353617 107354064 107358411
AMIL114957900 114960829
PDAM113666028 113679245
SPIS111325011 111325026 111331296 111331297 111337613 111345079
DGT114519758 114524348 114529411 114532953
XEN124433995 124434117 124452401 124458257
HMG100202915 100208555 105844712
HSY130625346 130635864 130657493
TADTRIADDRAFT_16140
AQU105315042 105315216 105315492 105315880 105316185 109583632 109586426 109588926
 » show all
Reference
1  [PMID:11123933]
  Authors
Krebs JF, Srinivasan A, Wong AM, Tomaselli KJ, Fritz LC, Wu JC.
  Title
Heavy membrane-associated caspase 3: identification, isolation, and characterization.
  Journal
Biochemistry 39:16056-63 (2000)
DOI:10.1021/bi001007w
Reference
2  [PMID:9261102]
  Authors
Li H, Bergeron L, Cryns V, Pasternack MS, Zhu H, Shi L, Greenberg A, Yuan J.
  Title
Activation of caspase-2 in apoptosis.
  Journal
J Biol Chem 272:21010-7 (1997)
DOI:10.1074/jbc.272.34.21010
Reference
3
  Authors
Nicholson, D. and Thornberry, N.A.
  Title
Caspase-3 and caspase-7.
  Journal
In: Barrett, A.J., Rawlings, N.D. and Woessner, J.F. (Eds.), Handbook of Proteolytic Enzymes, 2nd ed., Elsevier, London, 2004, p. 1298-1302.
Reference
4  [PMID:16781734]
  Authors
Fang B, Boross PI, Tozser J, Weber IT.
  Title
Structural and kinetic analysis of caspase-3 reveals role for s5 binding site in substrate recognition.
  Journal
J Mol Biol 360:654-66 (2006)
DOI:10.1016/j.jmb.2006.05.041
Reference
5  [PMID:11104820]
  Authors
Chang HY, Yang X.
  Title
Proteases for cell suicide: functions and regulation of caspases.
  Journal
Microbiol Mol Biol Rev 64:821-46 (2000)
DOI:10.1128/mmbr.64.4.821-846.2000
Reference
6  [PMID:8665848]
  Authors
Martin SJ, Amarante-Mendes GP, Shi L, Chuang TH, Casiano CA, O'Brien GA, Fitzgerald P, Tan EM, Bokoch GM, Greenberg AH, Green DR.
  Title
The cytotoxic cell protease granzyme B initiates apoptosis in a cell-free system by proteolytic processing and activation of the ICE/CED-3 family protease, CPP32, via a novel two-step mechanism.
  Journal
EMBO J 15:2407-16 (1996)
DOI:10.1002/j.1460-2075.1996.tb00598.x
Other DBs
ExplorEnz - The Enzyme Database: 3.4.22.56
IUBMB Enzyme Nomenclature: 3.4.22.56
ExPASy - ENZYME nomenclature database: 3.4.22.56
BRENDA, the Enzyme Database: 3.4.22.56
CAS: 169592-56-7
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