KEGG   ENZYME: 6.1.1.27
Entry
EC 6.1.1.27                 Enzyme                                 
Name
O-phospho-L-serine---tRNA ligase;
O-phosphoseryl-tRNA ligase;
non-canonical O-phosphoseryl-tRNA synthetase;
SepRS
Class
Ligases;
Forming carbon-oxygen bonds;
Ligases forming aminoacyl-tRNA and related compounds
Sysname
O-phospho-L-serine:tRNACys ligase (AMP-forming)
Reaction(IUBMB)
ATP + O-phospho-L-serine + tRNACys = AMP + diphosphate + O-phospho-L-seryl-tRNACys [RN:R08576]
Reaction(KEGG)
R08576
Substrate
ATP [CPD:C00002];
O-phospho-L-serine [CPD:C01005];
tRNA(Cys) [CPD:C01639]
Product
AMP [CPD:C00020];
diphosphate [CPD:C00013];
O-phospho-L-seryl-tRNACys
Comment
In organisms like Archaeoglobus fulgidus lacking EC 6.1.1.16 (cysteine---tRNA ligase) for the direct Cys-tRNACys formation, Cys-tRNACys is produced by an indirect pathway, in which EC 6.1.1.27 (O-phosphoseryl-tRNA ligase) ligates O-phosphoserine to tRNACys, and EC 2.5.1.73 (O-phospho-L-seryl-tRNA: Cys-tRNA synthase) converts the produced O-phospho-L-seryl-tRNACys to Cys-tRNACys. The SepRS/SepCysS pathway is the sole route for cysteine biosynthesis in the organism [1]. Methanosarcina mazei can use both pathways, the direct route using EC 6.1.1.16 (cysteine---tRNA ligase) and the indirect pathway with EC 6.1.1.27 and EC 2.5.1.73 (O-phospho-L-seryl-tRNA: Cys-tRNA synthase) [2].
History
EC 6.1.1.27 created 2009
Pathway
ec00970  Aminoacyl-tRNA biosynthesis
Orthology
K07587  O-phosphoseryl-tRNA synthetase
Genes
MJAMJ_1660
MFEMefer_0735
MVUMetvu_1155
MFSMFS40622_0592
MIFMetin_0235
MJHJH146_1468
MESGMLAUSG7_1103(sepS)
MESAMLASG1_0675(sepS)
MIGMetig_1346
MMPMMP0688
MMQMmarC5_0888
MMXMmarC6_0196
MMZMmarC7_1715
MMDGYY_02815
MMAKMMKA1_07400(sepS)
MMAOMMOS7_07340(sepS)
MMADMMJJ_02890(sepS)
MAEMaeo_1060
MVNMevan_1570
MVOMvol_0433
MOKMetok_0981
METFCFE53_05100(sepS)
MTHMTH_1501
MMGMTBMA_c00860(pheS1)
MWOMWSIV6_0042(sepS)
MTEEMTTB_00760
METCMTCT_1365
METEtca_01448(pheS_2)
METZMETMT2_1346
METKFVF72_04105(sepS)
MTHMFZP57_00155(sepS)
MAROMarbSA_11170
MELMetbo_0044
MEWMSWAN_0057
METHMBMB1_0036(sepS)
MFCBRM9_0091(sepS)
MFIDSM1535_1409(sepS)
MCUBMCBB_0052(sepS)
MSUBBK009_09900
METNBK008_05560
METTCIT01_05855(sepS)
METOCIT02_06885(sepS)
MEMEHYG87_06790(sepS)
MFVMfer_0342
MKAMK0439
AFUAF_0110
AFGAFULGI_00001070
APOArcpr_1311
AVEArcve_1107
ASTAsulf_01133
FPLFerp_0952
GACGACE_0149
GAHGAH_00998
MBAMbar_A1204
MBYMSBRM_3441
MBWMSBRW_3520
MBARMSBR2_3396
MBAKMSBR3_3408
MACMA_0090(pheRS)
MMAMM_1383
MMAZMmTuc01_1435
MMJMSMAS_3279
MMACMSMAC_3298
MVCMSVAZ_3488
MEKMSKOL_3456
MLSMSLAZ_3154
METMMSMTP_3173
MEFMSWH1_3131
MEQMSWHS_3343
MSJMSSAC_0069
MSZMSSIH_0062
MSWMSSIT_0062
MTHRMSTHT_1883
MTHEMSTHC_1401
MHORMSHOH_4028
MFZAOB57_008705
MBUMbur_0923
MMETMCMEM_1431
MELOJ7W08_05865(sepS)
MMHMmah_0685
MHAZBHR79_10180
MEVMetev_1721
MZHMzhil_0247
MPYMpsy_1453
MZIHWN40_01550
MHZMetho_0248
MTPMthe_0363
MCJMCON_3135(sepS)
MHIMhar_0928
MHUMhun_2839
MRTJKHC33_02760(sepS)
MLAMlab_0537
MEMMemar_2215
MBGBN140_1447
MEMAMMAB1_1853(sepS)
MCHKMchiMG62_25320
MSUMOH143_05355(sepS)
MPIMpet_0614
MENDL6E24_05485(sepS)
MAQERJ40_06815
MOUOU421_07565(sepS)
MBNMboo_0273
MFOMetfor_2843
MPLMpal_0290
MPDMCP_1457
MEZMtc_1161
RCIRRC273
MELUMTLP_06560
BARBAOA66_0046(sepS)
 » show all
Reference
1  [PMID:17351629]
  Authors
Fukunaga R, Yokoyama S
  Title
Structural insights into the first step of RNA-dependent cysteine biosynthesis in archaea.
  Journal
Nat Struct Mol Biol 14:272-9 (2007)
DOI:10.1038/nsmb1219
  Sequence
[mja:MJ_1660] [afu:AF_0110]
Reference
2  [PMID:18559341]
  Authors
Hauenstein SI, Perona JJ
  Title
Redundant synthesis of cysteinyl-tRNACys in Methanosarcina mazei.
  Journal
J Biol Chem 283:22007-17 (2008)
DOI:10.1074/jbc.M801839200
Other DBs
ExplorEnz - The Enzyme Database: 6.1.1.27
IUBMB Enzyme Nomenclature: 6.1.1.27
ExPASy - ENZYME nomenclature database: 6.1.1.27
BRENDA, the Enzyme Database: 6.1.1.27
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