| Entry |
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| Name |
aminopyrimidine aminohydrolase;
thiaminase (ambiguous);
thiaminase II;
tenA (gene name)
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| Class |
Hydrolases;
Acting on carbon-nitrogen bonds, other than peptide bonds;
In other compounds
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| Sysname |
4-amino-5-aminomethyl-2-methylpyrimidine aminohydrolase
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| Reaction(IUBMB) |
(1) 4-amino-5-aminomethyl-2-methylpyrimidine + H2O = 4-amino-5-hydroxymethyl-2-methylpyrimidine + NH3 [RN: R09993];
(2) thiamine + H2O = 4-amino-5-hydroxymethyl-2-methylpyrimidine + 5-(2-hydroxyethyl)-4-methylthiazole [RN: R02133]
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| Reaction(KEGG) |
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| Substrate |
4-amino-5-aminomethyl-2-methylpyrimidine [CPD: C20267];
H2O [CPD: C00001];
thiamine [CPD: C00378]
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| Product |
4-amino-5-hydroxymethyl-2-methylpyrimidine [CPD: C01279];
NH3 [CPD: C00014];
5-(2-hydroxyethyl)-4-methylthiazole [CPD: C04294]
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| Comment |
Previously known as thiaminase II, this enzyme is involved in the regeneration of the thiamine pyrimidine from degraded products, rather than in thiamine degradation, and participates in thiamine salvage pathways.
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| History |
EC 3.5.99.2 created 1961, modified 2011
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| Pathway |
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| Orthology |
| K00877 | hydroxymethylpyrimidine/phosphomethylpyrimidine kinase / thiaminase |
| K03707 | thiaminase (transcriptional activator TenA) |
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| Genes |
» show all
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| Reference |
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| Authors |
FUJITA A, NOSE Y, KURATANIK. |
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| Title |
The second type of bacterial thiaminase. |
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| Journal |
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| Reference |
2 |
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| Authors |
Ikehata, H. |
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| Title |
Purification of thiaminase II. |
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| Journal |
J Gen Appl Microbiol 6:30-39 (1960) |
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| Reference |
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| Authors |
Toms AV, Haas AL, Park JH, Begley TP, Ealick SE |
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| Title |
Structural characterization of the regulatory proteins TenA and TenI from Bacillus subtilis and identification of TenA as a thiaminase II. |
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| Journal |
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| Sequence |
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| Reference |
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| Authors |
Benach J, Edstrom WC, Lee I, Das K, Cooper B, Xiao R, Liu J, Rost B, Acton TB, Montelione GT, Hunt JF |
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| Title |
The 2.35 A structure of the TenA homolog from Pyrococcus furiosus supports an enzymatic function in thiamine metabolism. |
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| Journal |
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| Reference |
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| Authors |
Jenkins AH, Schyns G, Potot S, Sun G, Begley TP |
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| Title |
A new thiamin salvage pathway. |
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| Journal |
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| Reference |
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| Authors |
Jenkins AL, Zhang Y, Ealick SE, Begley TP |
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| Title |
Mutagenesis studies on TenA: a thiamin salvage enzyme from Bacillus subtilis. |
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| Journal |
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| Sequence |
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| Reference |
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| Authors |
French JB, Begley TP, Ealick SE |
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| Title |
Structure of trifunctional THI20 from yeast. |
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| Journal |
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| Sequence |
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| Other DBs |
| ExplorEnz - The Enzyme Database: | 3.5.99.2 |
| ExPASy - ENZYME nomenclature database: | 3.5.99.2 |
| UM-BBD (Biocatalysis/Biodegradation Database): | 3.5.99.2 |
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| LinkDB |
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