Entry
Name
endoplasmic reticulum Man9GlcNAc2 1,2-alpha-mannosidase;
MAN1B1 (gene name);
MNS1 (gene name);
MNS3 (gene name)
Class
Hydrolases;
Glycosylases;
Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
BRITE hierarchy
Sysname
Man9GlcNAc2-[protein]2-alpha-mannohydrolase (configuration-inverting)
Reaction(IUBMB)
Man9GlcNAc2-[protein] + H2O = Man8GlcNAc2-[protein] (isomer 8A1,2,3B1,3) + D-mannopyranose [RN:
R12477 ]
Reaction(KEGG)
Substrate
Man9GlcNAc2-[protein];
H2O [CPD:
C00001 ]
Product
Man8GlcNAc2-[protein] (isomer 8A1,2,3B1,3);
D-mannopyranose [CPD:
C00159 ]
Comment
The enzyme, located in the endoplasmic reticulum, primarily trims a single alpha-1,2-linked mannose residue from Man9GlcNAc2 to produce Man8GlcNAc2 isomer 8A1,2,3B1,3 (the names of the isomers listed here are based on a nomenclature system proposed by Prien et al [7]). The removal of the single mannosyl residue occurs in all eukaryotes as part of the processing of N-glycosylated proteins, and is absolutely essential for further elongation of the outer chain of properly-folded N-glycosylated proteins in yeast. In addition, the enzyme is involved in glycoprotein quality control at the ER quality control compartment (ERQC), helping to target misfolded glycoproteins for degradation. When present at very high concentrations in the ERQC, the enzyme can trim the carbohydrate chain further to Man(5-6)GlcNAc2.
History
EC 3.2.1.209 created 2019
Pathway
ec00513 Various types of N-glycan biosynthesis
Orthology
K23741 endoplasmic reticulum Man9GlcNAc2 1,2-alpha-mannosidase
Genes
NGI : 103732606 103741172(Man1b1)
UAH : 113248412 113248447 113248587 113248656 113250061
UAR : 123796283 123796320(MAN1B1)
ORO : 101377733 101380090 101380385
PYU : 121020233(MAN1B1) 121024619
AJU : 106989099(MAN1B1) 113597246
HHV : 120222776 120226452 120227604
BTA : 100336900(MAN1B1) 107131137(MAN1B1)
BBUB : 102392446 102401107
CHX : 102182048(MAN1B1) 106501835
OAS : 105605821 105605822(MAN1B1)
CCAD : 122442403 122442546(MAN1B1)
CDK : 105106772(MAN1B1) 105106783
BACU : 103003282 103008754(MAN1B1)
PCAD : 102987847 112065486
ECB : 100147103(MAN1B1) 100147680
PDIC : 114507914(MAN1B1) 118499400
LAV : 100664627(MAN1B1) 100670902
BGAR : 122919491 122946129
DRE : 556237(man1b1b) 562592(man1b1a)
SRX : 107709694 107736907 107739703 107757969
SANH : 107661470 107681241 107690671 107692283
SGH : 107548773 107560236 107587425 107595674
CCAR : 109045768 109052759 109081650
CAUA : 113038798 113049299 113064119 113074139
IPU : 108255760(man1b1b) 108279088(man1b1a)
PHYP : 113535490 113536854
SMEO : 124380546(man1b1b) 124398172(man1b1a)
AMEX : 103022471(man1b1) 103024141
EEE : 113573453 113577930(man1b1)
TNG : GSTEN00004960G001 GSTEN00009295G001
LCO : 104923806 104930877(man1b1)
CGOB : 115013303(man1b1) 115016675
ELY : 117251930(man1b1b) 117270132(man1b1a)
PLEP : 121944325 121959882(man1b1a)
SLUC : 116034794(man1b1a) 116050665(man1b1b)
ECRA : 117944690(man1b1b) 117959386(man1b1a)
PFLV : 114555183(man1b1) 114571584
GAT : 120830398(man1b1b) 120831937(man1b1a)
MSAM : 119883771 119892352 119914215 119917792
CUD : 121509526 121524878(man1b1a)
OAU : 116319660(man1b1a) 116332782(man1b1b)
OLA : 101156826(man1b1) 101168136
OML : 112137701(man1b1b) 112162853(man1b1a)
XMA : 102232900(man1b1) 102236792
PRET : 103469620 103473953
PFOR : 103141937 103155541
PLAI : 106936359 106951546
PMEI : 106904614 106906201
GAF : 122819517(man1b1a) 122827851(man1b1b)
CTUL : 119771995(man1b1a) 119789284(man1b1b)
GMU : 124872048(man1b1a) 124878115(man1b1b)
KMR : 108241416(man1b1a) 108245625(man1b1b)
ALIM : 106529420 106535448 106535552
AOCE : 111578422 111582694
CSEM : 103389019 103397364
POV : 109639093(man1b1) 109641232
SSEN : 122758578(man1b1a) 122769261
HHIP : 117767827(man1b1b) 117772246(man1b1a)
LCF : 108878392(man1b1) 108889011
SDU : 111221245 111226918(man1b1)
SLAL : 111647914(man1b1) 111650117
XGL : 120805701(man1b1b) 120806846(man1b1a)
BPEC : 110154656(man1b1) 110164288
MALB : 109959672 109968690
SASA : 106563131 106579997 106585427
OTW : 112232797 112234451(man1b1a) 112263106
OMY : 110509710(man1b1a) 110522854 110525472(man1b1b) 110535854
OGO : 124016952(man1b1a) 124033369 124036136
ONE : 115111439 115139862 115147146
SALP : 111960863 111963948 111974854(man1b1) 111974855
SNH : 120021738(man1b1a) 120025509 120054209 120063123
SFM : 108922690 108938629(man1b1)
PKI : 111854001 111857531(man1b1)
AANG : 118213031(man1b1a) 118237521(man1b1b)
ARUT : 117397023 117962293
DME : Dmel_CG11874(alpha-Man-Ib)
DSI : Dsimw501_GD17996(Dsim_GD17996)
HAME : 121860164 121875029
CSCU : 111620023 111631894
CEL : CELE_T03G11.4(mans-3)
BMY : BM_BM5524(Bma-mans-3)
HRO : HELRODRAFT_113976 HELRODRAFT_178867 HELRODRAFT_193110
PDAM : 113665639 113673540
CSAT : 104741591 104757343 104777030
BNA : 106361602 106367368 106375950 106425028 111212331
MINC : 123192943 123218754
GHI : 107905885 107958416 121230865
LJA : Lj6g3v1934090.1(Lj6g3v1934090.1)
CMAX : 111478371 111496964
CMOS : 111433252 111433756
CPEP : 111781525 111804180
MESC : 110601605 110605198
QSU : 111984426 112002631 112014039 112015529 112021377
PSOM : 113289725 113299707
DOSA : Os05t0209100-01(Os05g0209100)
TAES : 123041929 123116155 123180779
PVIR : 120663968 120697123
SMO : SELMODRAFT_168485 SELMODRAFT_176646
VCN : VOLCADRAFT_119746(amd1)
CCP : CHC_T00008598001 CHC_T00009079001
NCS : NCAS_0J02160(NCAS0J02160)
NDI : NDAI_0J02870(NDAI0J02870)
TPF : TPHA_0L00320(TPHA0L00320)
TBL : TBLA_0B06140(TBLA0B06140)
TDL : TDEL_0D00600(TDEL0D00600)
KAF : KAFR_0E04290(KAFR0E04290)
SPAA : SPAPADRAFT_57837(MNS1.1)
CAL : CAALFM_C103730CA(MNS1)
CTEN : CANTEDRAFT_110685(MNS1)
NTE : NEUTE1DRAFT74512(NEUTE1DRAFT_74512)
TTT : THITE_2057638 THITE_2085147
TMN : UCRPA7_1844 UCRPA7_7249
FPU : FPSE_04518 FPSE_04589
CFJ : CFIO01_01386 CFIO01_11192
ELA : UCREL1_10905 UCREL1_115
BFU : BCIN_01g06120(Bcmns1)
ANG : ANI_1_1456164(An18g06220)
AJE : HCAG_04182 HCAG_08449
BGH : BDBG_01802 BDBG_03796
ZTR : MYCGRDRAFT_45066(MgAMN9)
PFJ : MYCFIDRAFT_204581 MYCFIDRAFT_88410
PPL : POSPLDRAFT_111281 POSPLDRAFT_130143 POSPLDRAFT_49494 POSPLDRAFT_99932
TVS : TRAVEDRAFT_123722 TRAVEDRAFT_54985
PCO : PHACADRAFT_248627 PHACADRAFT_28202
SHS : STEHIDRAFT_63527 STEHIDRAFT_68181
HIR : HETIRDRAFT_30389 HETIRDRAFT_64121
PSQ : PUNSTDRAFT_113060 PUNSTDRAFT_140588
ADL : AURDEDRAFT_110124 AURDEDRAFT_112600
GTR : GLOTRDRAFT_105477 GLOTRDRAFT_134936
LBC : LACBIDRAFT_228999 LACBIDRAFT_255188 LACBIDRAFT_305436
MPR : MPER_07540 MPER_09462 MPER_10851 MPER_11974
MRR : Moror_11662 Moror_1765 Moror_9085
CCI : CC1G_00625 CC1G_12305
ABP : AGABI1DRAFT117691(AGABI1DRAFT_117691) AGABI1DRAFT130713(AGABI1DRAFT_130713) AGABI1DRAFT54540(AGABI1DRAFT_54540) AGABI1DRAFT70315(AGABI1DRAFT_70315)
ABV : AGABI2DRAFT176302(AGABI2DRAFT_176302) AGABI2DRAFT181939(AGABI2DRAFT_181939) AGABI2DRAFT206040(AGABI2DRAFT_206040) AGABI2DRAFT222683(AGABI2DRAFT_222683)
SMIN : v1.2.009441.t1(symbB.v1.2.009441.t1) v1.2.009441.t2(symbB.v1.2.009441.t2) v1.2.025226.t1(symbB.v1.2.025226.t1) v1.2.032603.t1(symbB.v1.2.032603.t1)
FCY : FRACYDRAFT_261302(MNS2_1)
NGD : NGA_2009400(MAN1) NGA_2024900
AAF : AURANDRAFT_10610 AURANDRAFT_71340
PIF : PITG_01855 PITG_10149
PSOJ : PHYSODRAFT_313654 PHYSODRAFT_557352
SPAR : SPRG_01621 SPRG_08812
EHX : EMIHUDRAFT_455715 EMIHUDRAFT_464075 EMIHUDRAFT_64898 EMIHUDRAFT_76998
TVA : TVAG_104870 TVAG_245880 TVAG_379870 TVAG_382860 TVAG_402460 TVAG_447400
» show all
Taxonomy
Reference
Authors
Jelinek-Kelly S, Herscovics A
Title
Glycoprotein biosynthesis in Saccharomyces cerevisiae. Purification of the alpha-mannosidase which removes one specific mannose residue from Man9GlcNAc.
Journal
J Biol Chem 263:14757-63 (1988)
Reference
Authors
Ziegler FD, Trimble RB
Title
Glycoprotein biosynthesis in yeast: purification and characterization of the endoplasmic reticulum Man9 processing alpha-mannosidase.
Journal
Reference
Authors
Gonzalez DS, Karaveg K, Vandersall-Nairn AS, Lal A, Moremen KW
Title
Identification, expression, and characterization of a cDNA encoding human endoplasmic reticulum mannosidase I, the enzyme that catalyzes the first mannose trimming step in mammalian Asn-linked oligosaccharide biosynthesis.
Journal
Sequence
Reference
Authors
Herscovics A, Romero PA, Tremblay LO
Title
The specificity of the yeast and human class I ER alpha 1,2-mannosidases involved in ER quality control is not as strict previously reported.
Journal
Glycobiology 12:14G-15G (2002)
Reference
Authors
Avezov E, Frenkel Z, Ehrlich M, Herscovics A, Lederkremer GZ
Title
Endoplasmic reticulum (ER) mannosidase I is compartmentalized and required for N-glycan trimming to Man5-6GlcNAc2 in glycoprotein ER-associated degradation.
Journal
Sequence
Reference
Authors
Liebminger E, Huttner S, Vavra U, Fischl R, Schoberer J, Grass J, Blaukopf C, Seifert GJ, Altmann F, Mach L, Strasser R
Title
Class I alpha-mannosidases are required for N-glycan processing and root development in Arabidopsis thaliana.
Journal
Sequence
Reference
Authors
Prien JM, Ashline DJ, Lapadula AJ, Zhang H, Reinhold VN
Title
The high mannose glycans from bovine ribonuclease B isomer characterization by ion trap MS.
Journal
Other DBs
ExPASy - ENZYME nomenclature database: 3.2.1.209
LinkDB
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