KEGG   PATHWAY: cfi03030
Entry
cfi03030                    Pathway                                
Name
DNA replication - Cellulomonas fimi
Description
A complex network of interacting proteins and enzymes is required for DNA replication. Generally, DNA replication follows a multistep enzymatic pathway. At the DNA replication fork, a DNA helicase (DnaB or MCM complex) precedes the DNA synthetic machinery and unwinds the duplex parental DNA in cooperation with the SSB or RPA. On the leading strand, replication occurs continuously in a 5 to 3 direction, whereas on the lagging strand, DNA replication occurs discontinuously by synthesis and joining of short Okazaki fragments. In prokaryotes, the leading strand replication apparatus consists of a DNA polymerase (pol III core), a sliding clamp (beta), and a clamp loader (gamma delta complex). The DNA primase (DnaG) is needed to form RNA primers. Normally, during replication of the lagging-strand DNA template, an RNA primer is removed either by an RNase H or by the 5 to 3 exonuclease activity of DNA pol I, and the DNA ligase joins the Okazaki fragments. In eukaryotes, three DNA polymerases (alpha, delta, and epsilon) have been identified. DNA primase forms a permanent complex with DNA polymerase alpha. PCNA and RFC function as a clamp and a clamp loader. FEN 1 and RNase H1 remove the RNA from the Okazaki fragments and DNA ligase I joins the DNA.
Class
Genetic Information Processing; Replication and repair
Pathway map
cfi03030  DNA replication
cfi03030

Organism
Cellulomonas fimi [GN:cfi]
Gene
Celf_1074  Exonuclease RNase T and DNA polymerase III [KO:K02342] [EC:2.7.7.7]
Celf_2178  DNA polymerase III, epsilon subunit [KO:K02342] [EC:2.7.7.7]
Celf_1627  DNA polymerase III, alpha subunit [KO:K02337] [EC:2.7.7.7]
Celf_3419  DNA polymerase III, subunits gamma and tau [KO:K02343] [EC:2.7.7.7]
Celf_0483  AAA ATPase [KO:K02341] [EC:2.7.7.7]
Celf_2369  DNA polymerase III, delta subunit [KO:K02340] [EC:2.7.7.7]
Celf_0002  DNA polymerase III, beta subunit [KO:K02338] [EC:2.7.7.7]
Celf_0053  replicative DNA helicase [KO:K02314] [EC:5.6.2.3]
Celf_2304  DNA primase [KO:K02316] [EC:2.7.7.101]
Celf_1383  single-strand binding protein [KO:K03111]
Celf_3783  single-strand binding protein [KO:K03111]
Celf_0537  ribonuclease H [KO:K03469] [EC:3.1.26.4]
Celf_2257  Phosphoglycerate mutase [KO:K22316] [EC:3.1.26.4 3.1.3.73]
Celf_1507  Ribonuclease H [KO:K03470] [EC:3.1.26.4]
Celf_1839  DNA polymerase I [KO:K02335] [EC:2.7.7.7]
Celf_2751  DNA ligase, NAD-dependent [KO:K01972] [EC:6.5.1.2]
Reference
PMID:8087839
  Authors
Stillman B.
  Title
Smart machines at the DNA replication fork.
  Journal
Cell 78:725-8 (1994)
DOI:10.1016/S0092-8674(94)90362-X
Reference
PMID:9759502
  Authors
Waga S, Stillman B.
  Title
The DNA replication fork in eukaryotic cells.
  Journal
Annu Rev Biochem 67:721-51 (1998)
DOI:10.1146/annurev.biochem.67.1.721
KO pathway
ko03030   
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