Entry
Name
Biofilm formation - Vibrio cholerae
Description
Surface colonization and subsequent biofilm formation and development provide numerous advantages to microorganisms. On the other hand, biofilm formation is an energetically costly process and therefore must be tightly regulated and plastic, enabling biofilm bacteria to be responsive to the various environmental cues. The quorum sensing (QS) pathway of Vibrio cholerae activates the expression of VpsR, VpsT and AphA (the main activators of biofilm formation) at low cell density and HapR (the main repressor) at high cell density. At low cell density, biofilm genes, including Vibrio polysaccharide (VPS) biosynthesis genes and the major extracellular matrix genes RbmA, RbmC, and Bap1, are expressed. The QS pathway also leads to induction of virulence factors such as toxin coregulated pilus (TCP) and cholera toxin (CTX), essential for colonization of the host and enterotoxicity, respectively. The VarS/VarA signaling system responds to an unknown environmental cue and represses biofilm production by process which involves the regulatory sRNAs CsrB, CsrC and CsrD and the RNA-binding protein CsrA, interfering with LuxO of the QS pathway. The second messenger cAMP acts as a repressor of biofilm formation. cAMP is generated by adenylate cyclase CyaA, and in complex with cAMP receptor protein (CRP), this nucleotide has been shown to upregulate HapR production through the positive regulation of the CAI-I synthase CqsA and the negative regulation of Fis. The second messenger c-di-GMP is a key signalling molecule controlling motility and biofilm matrix production. High cellular levels of c-di-GMP promote enhanced transcription of genes involved in biofilm formation, possibly by promoting VpsT-mediated transcriptional activation of vps genes.
Class
Cellular Processes; Cellular community - prokaryotes
BRITE hierarchy
Pathway map
ko05111 Biofilm formation - Vibrio cholerae
Ortholog table
Module
M00852 Vibrio cholerae pathogenicity signature, toxin coregulated pilus [PATH:ko05111 ]
Orthology
K10909 two-component system, autoinducer 2 sensor kinase/phosphatase LuxQ [EC:2.7.13.3 3.1.3.-]
K10910 autoinducer 2-binding periplasmic protein LuxP
K10915 CAI-1 autoinducer synthase [EC:2.3.-.-]
K10916 two-component system, CAI-1 autoinducer sensor kinase/phosphatase CqsS [EC:2.7.13.3 3.1.3.-]
K10911 two-component system, phosphorelay protein LuxU
K10912 two-component system, repressor protein LuxO
K15851 quorum regulatory RNA Qrr
K10917 PadR family transcriptional regulator, regulatory protein AphA
K10918 LysR family transcriptional regulator, transcriptional activator AphB
K20918 LuxR family transcriptional regulator, positive regulator of biofilm formation
K20919 sigma-54 dependent transcriptional regulator, positive regulator of biofilm formation
K01791 UDP-N-acetylglucosamine 2-epimerase (non-hydrolysing) [EC:5.1.3.14 ]
K20921 polysaccharide biosynthesis protein VpsD
K20946 polysaccharide biosynthesis protein VpsE
K20947 polysaccharide biosynthesis protein VpsF
K20922 polysaccharide biosynthesis protein VpsI
K20948 polysaccharide biosynthesis protein VpsJ
K25886 polysaccharide biosynthesis protein VpsK
K03606 undecaprenyl-phosphate glucose phosphotransferase [EC:2.7.8.31 ]
K20920 polysaccharide biosynthesis protein VpsM
K20988 polysaccharide biosynthesis/export protein VpsN
K20949 polysaccharide biosynthesis protein VpsP
K20950 polysaccharide biosynthesis protein VpsQ
K02452 general secretion pathway protein C
K02453 general secretion pathway protein D
K02455 general secretion pathway protein F
K02456 general secretion pathway protein G
K02457 general secretion pathway protein H
K02458 general secretion pathway protein I
K02459 general secretion pathway protein J
K02460 general secretion pathway protein K
K02461 general secretion pathway protein L
K02462 general secretion pathway protein M
K02463 general secretion pathway protein N
K20951 extracellular matrix protein
K20952 rugosity and biofilm structure modulator C
K20953 rugosity and biofilm structure modulator A
K10920 toxin coregulated pilus biosynthesis protein P
K10919 toxin coregulated pilus biosynthesis protein H
K10921 cholera toxin transcriptional activator
K10922 transmembrane regulatory protein ToxS
K10923 AraC family transcriptional regulator, TCP pilus virulence regulatory protein
K10940 outer membrane protein OmpT
K08720 outer membrane protein OmpU
K10930 toxin coregulated pilin
K10931 toxin coregulated pilus biosynthesis protein B
K10932 toxin coregulated pilus biosynthesis outer membrane protein C
K10933 toxin coregulated pilus biosynthesis protein D
K10934 toxin coregulated pilus biosynthesis protein E
K10935 toxin coregulated pilus biosynthesis protein F
K10961 toxin coregulated pilus biosynthesis protein I
K10962 toxin coregulated pilus biosynthesis protein Q
K10963 toxin coregulated pilus biosynthesis protein R
K10964 toxin coregulated pilus biosynthesis protein S
K10965 toxin coregulated pilus biosynthesis protein T
K10936 accessory colonization factor AcfA
K10937 accessory colonization factor AcfB
K10938 accessory colonization factor AcfC
K10939 accessory colonization factor AcfD
K10929 cholera enterotoxin subunit B
K10913 TetR/AcrR family transcriptional regulator, hemagglutinin/protease regulatory protein
K07678 two-component system, NarL family, sensor histidine kinase BarA [EC:2.7.13.3 ]
K07689 two-component system, NarL family, invasion response regulator UvrY
K18515 small regulatory RNA CsrB
K03563 carbon storage regulator
K02777 sugar PTS system EIIA component [EC:2.7.1.-]
K10914 CRP/FNR family transcriptional regulator, cyclic AMP receptor protein
K03557 Fis family transcriptional regulator, factor for inversion stimulation protein
K12276 MSHA biogenesis protein MshE
K10924 MSHA pilin protein MshA
K10925 MSHA pilin protein MshB
K10926 MSHA pilin protein MshC
K10927 MSHA pilin protein MshD
K10941 sigma-54 dependent transcriptional regulator, flagellar regulatory protein
K03092 RNA polymerase sigma-54 factor
K03087 RNA polymerase nonessential primary-like sigma factor
K10942 two-component system, sensor histidine kinase FlrB [EC:2.7.13.3 ]
K10943 two-component system, response regulator FlrC
K02405 RNA polymerase sigma factor FliA
Compound
C18076 5'-Phosphoguanylyl(3'->5')guanosine
Reference
Authors
Teschler JK, Zamorano-Sanchez D, Utada AS, Warner CJ, Wong GC, Linington RG, Yildiz FH
Title
Living in the matrix: assembly and control of Vibrio cholerae biofilms.
Journal
Reference
Authors
Dang H, Lovell CR
Title
Microbial Surface Colonization and Biofilm Development in Marine Environments.
Journal
Reference
Authors
Hengge R
Title
Principles of c-di-GMP signalling in bacteria.
Journal
Reference
Authors
Liang ZX
Title
The expanding roles of c-di-GMP in the biosynthesis of exopolysaccharides and secondary metabolites.
Journal
Reference
Authors
Sandkvist M, Michel LO, Hough LP, Morales VM, Bagdasarian M, Koomey M, DiRita VJ, Bagdasarian M
Title
General secretion pathway (eps) genes required for toxin secretion and outer membrane biogenesis in Vibrio cholerae.
Journal
Reference
Authors
Lenz DH, Miller MB, Zhu J, Kulkarni RV, Bassler BL
Title
CsrA and three redundant small RNAs regulate quorum sensing in Vibrio cholerae.
Journal
Reference
Authors
Houot L, Watnick PI
Title
A novel role for enzyme I of the Vibrio cholerae phosphoenolpyruvate phosphotransferase system in regulation of growth in a biofilm.
Journal
Reference
Authors
Liang W, Silva AJ, Benitez JA
Title
The cyclic AMP receptor protein modulates colonial morphology in Vibrio cholerae.
Journal
Reference
Authors
Lenz DH, Bassler BL
Title
The small nucleoid protein Fis is involved in Vibrio cholerae quorum sensing.
Journal
Related pathway
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