KEGG   PATHWAY: mtun03030
Entry
mtun03030                   Pathway                                
Name
DNA replication - Methylocella tundrae
Description
A complex network of interacting proteins and enzymes is required for DNA replication. Generally, DNA replication follows a multistep enzymatic pathway. At the DNA replication fork, a DNA helicase (DnaB or MCM complex) precedes the DNA synthetic machinery and unwinds the duplex parental DNA in cooperation with the SSB or RPA. On the leading strand, replication occurs continuously in a 5 to 3 direction, whereas on the lagging strand, DNA replication occurs discontinuously by synthesis and joining of short Okazaki fragments. In prokaryotes, the leading strand replication apparatus consists of a DNA polymerase (pol III core), a sliding clamp (beta), and a clamp loader (gamma delta complex). The DNA primase (DnaG) is needed to form RNA primers. Normally, during replication of the lagging-strand DNA template, an RNA primer is removed either by an RNase H or by the 5 to 3 exonuclease activity of DNA pol I, and the DNA ligase joins the Okazaki fragments. In eukaryotes, three DNA polymerases (alpha, delta, and epsilon) have been identified. DNA primase forms a permanent complex with DNA polymerase alpha. PCNA and RFC function as a clamp and a clamp loader. FEN 1 and RNase H1 remove the RNA from the Okazaki fragments and DNA ligase I joins the DNA.
Class
Genetic Information Processing; Replication and repair
Pathway map
mtun03030  DNA replication
mtun03030

Organism
Methylocella tundrae [GN:mtun]
Gene
MTUNDRAET4_0108.1  Exonuclease RNase T and DNA polymerase III [KO:K02342] [EC:2.7.7.7]
MTUNDRAET4_3027  dnaQ; DNA polymerase III subunit epsilon [KO:K02342] [EC:2.7.7.7]
MTUNDRAET4_3424  dnaE; DNA polymerase III subunit alpha [KO:K02337] [EC:2.7.7.7]
MTUNDRAET4_0411  dnaX; DNA polymerase III subunit gamma/tau [KO:K02343] [EC:2.7.7.7]
MTUNDRAET4_1133  AAA ATPase [KO:K02341] [EC:2.7.7.7]
MTUNDRAET4_0477  DNA polymerase III, delta subunit (modular protein) [KO:K02340] [EC:2.7.7.7]
MTUNDRAET4_3856  DNA polymerase III chi subunit HolC [KO:K02339] [EC:2.7.7.7]
MTUNDRAET4_2244  dnaN; Beta sliding clamp [KO:K02338] [EC:2.7.7.7]
MTUNDRAET4_0853  dnaB; Replicative DNA helicase [KO:K02314] [EC:5.6.2.3]
MTUNDRAET4_3504  dnaG; DNA primase [KO:K02316] [EC:2.7.7.101]
MTUNDRAET4_0104.2  Single-strand binding protein family protein [KO:K03111]
MTUNDRAET4_3839  ssb; Single-stranded DNA-binding protein [KO:K03111]
MTUNDRAET4_0992  ssb; Single-stranded DNA-binding protein [KO:K03111]
MTUNDRAET4_0533  rnhA; ribonuclease HI, degrades RNA of DNA-RNA hybrids [KO:K03469] [EC:3.1.26.4]
MTUNDRAET4_3551  rnhB; ribonuclease HII, degrades RNA of DNA-RNA hybrids [KO:K03470] [EC:3.1.26.4]
MTUNDRAET4_2137  polA; DNA polymerase I [KO:K02335] [EC:2.7.7.7]
MTUNDRAET4_1802  ligA; DNA ligase, NAD(+)-dependent [KO:K01972] [EC:6.5.1.2]
Reference
PMID:8087839
  Authors
Stillman B.
  Title
Smart machines at the DNA replication fork.
  Journal
Cell 78:725-8 (1994)
DOI:10.1016/S0092-8674(94)90362-X
Reference
PMID:9759502
  Authors
Waga S, Stillman B.
  Title
The DNA replication fork in eukaryotic cells.
  Journal
Annu Rev Biochem 67:721-51 (1998)
DOI:10.1146/annurev.biochem.67.1.721
KO pathway
ko03030   
LinkDB

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