Entry Name Cell cycle - yeast - Neurospora tetrasperma
Description Mitotic cell cycle progression is accomplished through a reproducible sequence of events, DNA replication (S phase) and mitosis (M phase) separated temporally by gaps (G1 and G2 phases). G1, S, and G2 phases are collectively known as interphase. In yeast Cdc28 is the catalytic subunit of the cyclin-dependent kinase (CDK). At G1 phase Cdc28 associates with G1-cyclins Cln1 to Cln3, while B-type cyclins Clb1 to Clb6 regulate Cdc28 during S, G2, and M phases. Cln3/Cdc28 activity is required for cells to pass through 'start', the commitment point in G1. When Cln3/Cdc28 accumulates more than a certain threshold, SBF (Swi4/Swi6) and MBF (Mbp1/Swi6) are activated, promoting transcription of Cln1, Cln2, and other genes required for S-phase progression. Cln1 and Cln2 interacting with Cdc28 promote activation of B-type cyclin associated CDK, which drives DNA replication and entry into mitosis. Specifically, Cdc28 association with Clb2 and Clb1 promotes entry into mitosis. Cells suffering from DNA damage, spindle misorientation, or spindle assembly defect do not undergo the metaphase-anaphase transition for chromosome segregation and fail to exit from mitosis. The spindle assembly checkpoint activates Mad2, which in turn prevents chromosome segregation by inhibiting degradation of the securin Pds1. Moreover, Pds1 is phosphorylated and stabilized in response to DNA damage in a Chk1-dependent manner. The spindle checkpoint is also involved in the Cdc14 release from the nucleolus. Cdc14 dephosphorylates Swi5, Sic1, and Cdh1, leading to inhibition of Cdc28 and degradation of cyclin required for mitotic exit.
Class Cellular Processes; Cell growth and deathBRITE hierarchy
Pathway map Other DBs Organism Neurospora tetrasperma [GN:
nte ]
Gene Compound Reference Authors Devault A, Vallen EA, Yuan T, Green S, Bensimon A, Schwob E
Title Identification of Tah11/Sid2 as the ortholog of the replication licensing factor Cdt1 in Saccharomyces cerevisiae.
Journal Reference Authors de Bruin RA, McDonald WH, Kalashnikova TI, Yates J 3rd, Wittenberg C
Title Cln3 activates G1-specific transcription via phosphorylation of the SBF bound repressor Whi5.
Journal Reference Authors Costanzo M, Nishikawa JL, Tang X, Millman JS, Schub O, Breitkreuz K, Dewar D, Rupes I, Andrews B, Tyers M
Title CDK activity antagonizes Whi5, an inhibitor of G1/S transcription in yeast.
Journal Reference Authors Pramila T, Miles S, GuhaThakurta D, Jemiolo D, Breeden LL
Title Conserved homeodomain proteins interact with MADS box protein Mcm1 to restrict ECB-dependent transcription to the M/G1 phase of the cell cycle.
Journal Reference Authors Schwickart M, Havlis J, Habermann B, Bogdanova A, Camasses A, Oelschlaegel T, Shevchenko A, Zachariae W
Title Swm1/Apc13 is an evolutionarily conserved subunit of the anaphase-promoting complex stabilizing the association of Cdc16 and Cdc27.
Journal Reference Authors Penkner AM, Prinz S, Ferscha S, Klein F
Title Mnd2, an essential antagonist of the anaphase-promoting complex during meiotic prophase.
Journal Reference Authors Pesin JA, Orr-Weaver TL
Title Regulation of APC/C activators in mitosis and meiosis.
Journal Reference Authors Dirick L, Bohm T, Nasmyth K
Title Roles and regulation of Cln-Cdc28 kinases at the start of the cell cycle of Saccharomyces cerevisiae.
Journal Reference Authors Bahler J
Title Cell-cycle control of gene expression in budding and fission yeast.
Journal Reference Authors Peter M, Herskowitz I
Title Direct inhibition of the yeast cyclin-dependent kinase Cdc28-Cln by Far1.
Journal Reference Authors Henchoz S, Chi Y, Catarin B, Herskowitz I, Deshaies RJ, Peter M
Title Phosphorylation- and ubiquitin-dependent degradation of the cyclin-dependent kinase inhibitor Far1p in budding yeast.
Journal Reference Authors Feldman RM, Correll CC, Kaplan KB, Deshaies RJ
Title A complex of Cdc4p, Skp1p, and Cdc53p/cullin catalyzes ubiquitination of the phosphorylated CDK inhibitor Sic1p.
Journal Reference Authors Schwob E, Bohm T, Mendenhall MD, Nasmyth K
Title The B-type cyclin kinase inhibitor p40SIC1 controls the G1 to S transition in S. cerevisiae.
Journal Reference Authors Mendenhall MD, Hodge AE
Title Regulation of Cdc28 cyclin-dependent protein kinase activity during the cell cycle of the yeast Saccharomyces cerevisiae.
Journal Microbiol Mol Biol Rev 62:1191-243 (1998)
Reference Authors Zachariae W, Nasmyth K
Title Whose end is destruction: cell division and the anaphase-promoting complex.
Journal Reference Authors Jares P, Donaldson A, Blow JJ
Title The Cdc7/Dbf4 protein kinase: target of the S phase checkpoint?
Journal Reference Authors Elsasser S, Chi Y, Yang P, Campbell JL
Title Phosphorylation controls timing of Cdc6p destruction: A biochemical analysis.
Journal Reference Authors Skowyra D, Koepp DM, Kamura T, Conrad MN, Conaway RC, Conaway JW, Elledge SJ, Harper JW
Title Reconstitution of G1 cyclin ubiquitination with complexes containing SCFGrr1 and Rbx1.
Journal Reference Authors Mouillon JM, Persson BL
Title New aspects on phosphate sensing and signalling in Saccharomyces cerevisiae.
Journal Reference Authors Bean JM, Siggia ED, Cross FR
Title High functional overlap between MluI cell-cycle box binding factor and Swi4/6 cell-cycle box binding factor in the G1/S transcriptional program in Saccharomyces cerevisiae.
Journal Reference Authors Deshaies RJ
Title Phosphorylation and proteolysis: partners in the regulation of cell division in budding yeast.
Journal Reference Authors Asano S, Park JE, Sakchaisri K, Yu LR, Song S, Supavilai P, Veenstra TD, Lee KS
Title Concerted mechanism of Swe1/Wee1 regulation by multiple kinases in budding yeast.
Journal Reference Authors Kaiser P, Sia RA, Bardes EG, Lew DJ, Reed SI
Title Cdc34 and the F-box protein Met30 are required for degradation of the Cdk-inhibitory kinase Swe1.
Journal Reference Authors Barral Y, Parra M, Bidlingmaier S, Snyder M
Title Nim1-related kinases coordinate cell cycle progression with the organization of the peripheral cytoskeleton in yeast.
Journal Reference Authors Taylor SS
Title Chromosome segregation: dual control ensures fidelity.
Journal Reference Authors Lew DJ, Burke DJ
Title The spindle assembly and spindle position checkpoints.
Journal Reference Authors Simanis V
Title Events at the end of mitosis in the budding and fission yeasts.
Journal Reference Authors Toyn JH, Johnston LH
Title The Dbf2 and Dbf20 protein kinases of budding yeast are activated after the metaphase to anaphase cell cycle transition.
Journal Reference Authors Amon A
Title Together until separin do us part.
Journal Reference Authors Ciosk R, Shirayama M, Shevchenko A, Tanaka T, Toth A, Shevchenko A, Nasmyth K
Title Cohesin's binding to chromosomes depends on a separate complex consisting of Scc2 and Scc4 proteins.
Journal Reference Authors Freeman L, Aragon-Alcaide L, Strunnikov A
Title The condensin complex governs chromosome condensation and mitotic transmission of rDNA.
Journal Reference Authors Fu Y, Pastushok L, Xiao W
Title DNA damage-induced gene expression in Saccharomyces cerevisiae.
Journal Reference Authors Friedel AM, Pike BL, Gasser SM
Title ATR/Mec1: coordinating fork stability and repair.
Journal Reference Authors Chen SH, Zhou H
Title Reconstitution of Rad53 activation by Mec1 through adaptor protein Mrc1.
Journal Reference Authors Amon A
Title A decade of Cdc14--a personal perspective. Delivered on 9 July 2007 at the 32nd FEBS Congress in Vienna, Austria.
Journal Related KO pathway
