PATHWAY: plu00541 Help
Entry
Name
O-Antigen nucleotide sugar biosynthesis - Photorhabdus laumondii subsp. laumondii TTO1
Description
The O-antigen is a repetitive glycan polymer with a repeat unit of two to six sugar residues, and comprises the outermost and immunogenic domain of the lipopolysaccharide (
map00540 ). Most of the sugar residues are derived from nucleotide sugars and the genes for their biosynthesis seem to be well conserved among Gram-negative bacterial species. They include UDP-sugars synthesized from Glc-1P via UDP-Glc or from GlcNAc-1P via UDP-GlcNAc, GDP-sugars synthesized from Fru-6P via GDP-Man, and dTDP-sugars and CDP-sugars synthesized from Glc-1P via dTDP-Glc and CDP-Glc, respectively. CMP-sugars are also synthesized from UDP-GlcNAc. The O-antigen nucleotide sugar biosynthesis genes often form gene clusters with glycosyltransferase genes and other processing genes such as for translocation and polymerization.
Class
Metabolism; Glycan biosynthesis and metabolism
BRITE hierarchy
Pathway map
plu00541 O-Antigen nucleotide sugar biosynthesis
Ortholog table
Organism
Photorhabdus laumondii subsp. laumondii TTO1 [GN:
plu ]
Gene
plu2501 galU; UTP--glucose-1-phosphate uridylyltransferase (UDP-glucose pyrophosphorylase) (UDPGP) [KO:K00963 ] [EC:2.7.7.9 ]
plu4831 galE; UDP-glucose 4-epimerase (Galactowaldenase) (UDP-galactose 4-epimerase) [KO:K01784 ] [EC:5.1.3.2 ]
plu2500 unnamed protein product; Highly similar to probable nucleotide sugar dehydrogenase and UDP-glucose dehydrogenase [KO:K00012 ] [EC:1.1.1.22 ]
plu2499 unnamed protein product; Similar to unknown protein WbnF of Escherichia coli and to nucleotide sugar epimerase [KO:K08679 ] [EC:5.1.3.6 ]
plu4657 rffH; glucose-1-phosphate thymidylyltransferase (dTDP-glucose synthase) (dTDP-glucose pyrophosphorylase) [KO:K00973 ] [EC:2.7.7.24 ]
plu2360 manA; mannose-6-phosphate isomerase (phosphomannose isomerase) (phosphohexomutase) (PMI) [KO:K01809 ] [EC:5.3.1.8 ]
plu2404 unnamed protein product; Some similarities with aspartate aminotransferase and perosamine synthetase. Two candidate membrane-spanning segments [KO:K13010 ] [EC:2.6.1.102 ]
plu4659 wecC; UDP-N-acetyl-D-mannosaminuronic acid dehydrogenase (UDP-ManNAca dehydrogenase) [KO:K02472 ] [EC:1.1.1.336 ]
Compound
C00043 UDP-N-acetyl-alpha-D-glucosamine
C00128 CMP-N-acetylneuraminate
C00203 UDP-N-acetyl-D-galactosamine
C00688 dTDP-4-dehydro-beta-L-rhamnose
C01170 UDP-N-acetyl-D-mannosamine
C01219 CDP-4-dehydro-6-deoxy-D-glucose
C01222 GDP-4-dehydro-6-deoxy-D-mannose
C03187 dTDP-6-deoxy-beta-L-talose
C03598 CDP-3,6-dideoxy-D-glucose
C03599 CDP-3,6-dideoxy-D-mannose
C03733 UDP-alpha-D-galactofuranose
C04268 dTDP-4-amino-4,6-dideoxy-D-glucose
C04297 CDP-4-dehydro-3,6-dideoxy-D-glucose
C04346 dTDP-4-amino-4,6-dideoxy-D-galactose
C04501 N-Acetyl-alpha-D-glucosamine 1-phosphate
C04573 UDP-N-acetyl-2-amino-2-deoxy-D-glucuronate
C04613 UDP-2-acetamido-4-dehydro-2,6-dideoxyglucose
C04630 UDP-2-acetamido-4-amino-2,4,6-trideoxy-alpha-D-glucose
C06018 dTDP-4-acetamido-4,6-dideoxy-D-glucose
C06240 UDP-N-acetyl-D-mannosaminouronate
C11907 dTDP-4-oxo-6-deoxy-D-glucose
C11908 3,6-Dideoxy-3-oxo-dTDP-D-glucose
C11925 dTDP-3-amino-3,6-dideoxy-D-glucose
C13952 UDP-N-acetyl-D-galactosaminuronic acid
C17326 CDP-4-dehydro-3,6-dideoxy-D-glucose epimer
C19725 UDP-2,3-diacetamido-2,3-dideoxy-alpha-D-glucuronate
C19745 UDP-2,3-diacetamido-2,3-dideoxy-alpha-D-mannuronate
C19823 UDP-2-acetamido-2,6-dideoxy-beta-L-arabino-hexos-4-ulose
C19947 dTDP-3-amino-3,6-dideoxy-alpha-D-galactopyranose
C19960 dTDP-3-dehydro-6-deoxy-alpha-D-galactopyranose
C19961 UDP-4-amino-4,6-dideoxy-N-acetyl-beta-L-altrosamine
C19971 UDP-2,4-bis(acetamido)-2,4,6-trideoxy-beta-L-altropyranose
C19972 2,4-Bis(acetamido)-2,4,6-trideoxy-beta-L-altropyranose
C20156 dTDP-3-acetamido-3,6-dideoxy-alpha-D-galactopyranose
C20359 UDP-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucuronate
C20395 UDP-2-acetamido-2-deoxy-alpha-D-ribo-hex-3-uluronate
C20415 dTDP-4-acetamido-4,6-dideoxy-alpha-D-galactose
C20612 GDP-4-dehydro-3,6-dideoxy-alpha-D-mannose
C20638 GDP-4-amino-4,6-dideoxy-alpha-D-mannose
C20672 GDP-4-acetamido-4,6-dideoxy-alpha-D-mannose
C20682 dTDP-alpha-D-fucofuranose
C20768 UDP-2-acetamido-2,6-dideoxy-beta-L-talose
C20769 UDP-2-acetamido-2,6-dideoxy-beta-L-lyxo-4-hexulose
C20774 UDP-N-acetyl-beta-L-fucosamine
C22245 UDP-2-acetamido-2-deoxy-alpha-D-galacturonamide
C22246 UDP-2,3-dideoxy-2-acetamido-3-acetamidino-alpha-D-mannuronic acid
C22247 UDP-2-acetamido-4-(D-alanylamino)-2,4,6-trideoxy-alpha-D-glucopyranose
C22248 UDP-2-acetamido-4-(D-alanylamino)-2,4,6-trideoxy-alpha-D-mannopyranose
C22249 2-Acetamido-4-(D-alanylamino)-2,4,6-trideoxy-D-mannopyranose
C22250 5-N-Acetyl-7-N-(D-alanyl)-legionaminic acid
C22251 CMP-5-N-acetyl-7-N-(D-alanyl)-legionaminic acid
C22252 UDP-2,4-diacetamido-2,4,6-trideoxy-beta-L-idose
C22253 UDP-2,4-diacetamido-2,4,6-trideoxy-beta-L-gulose
C22254 2,4-Diacetamido-2,4,6-trideoxy-beta-L-gulose
C22255 5,7-Diacetamido-3,5,7,9-tetradeoxy-L-glycero-D-galacto-non-2-ulosonic acid
C22256 CMP-5,7-diacetamido-3,5,7,9-tetradeoxy-L-glycero-D-galacto-non-2-ulosonic acid
C22257 dTDP-3-acetamido-3,6-dideoxy-alpha-D-glucose
C22259 UDP-2-acetamido-2,6-dideoxy-beta-L-mannose
C22260 UDP-2-acetamido-2,6-dideoxy-beta-L-glucose
C22261 UDP-2-acetamido-2,6-dideoxy-alpha-D-galactose
C22262 UDP-2-acetamido-2,6-dideoxy-alpha-D-glucose
C22270 UDP-2,6-dideoxy-2-acetamidino-beta-L-galactose
C22276 UDP-4-amino-4,6-dideoxy-N-acetyl-beta-L-idosamine
C22397 GDP-N-formyl-alpha-D-perosamine
C22575 UDP-2-acetamido-4-amino-2,4,6-trideoxy-D-galactose
C22576 UDP-2-acetamido-2-deoxy-L-altruronic acid
Reference
Authors
Samuel G, Reeves P
Title
Biosynthesis of O-antigens: genes and pathways involved in nucleotide sugar precursor synthesis and O-antigen assembly.
Journal
Reference
Authors
Burrows LL, Charter DF, Lam JS
Title
Molecular characterization of the Pseudomonas aeruginosa serotype O5 (PAO1) B-band lipopolysaccharide gene cluster.
Journal
Reference
Authors
Belanger M, Burrows LL, Lam JS
Title
Functional analysis of genes responsible for the synthesis of the B-band O antigen of Pseudomonas aeruginosa serotype O6 lipopolysaccharide.
Journal
Reference
Authors
Dean CR, Goldberg JB
Title
The wbpM gene in Pseudomonas aeruginosa serogroup O17 resides on a cryptic copy of the serogroup O11 O antigen gene locus.
Journal
Reference
Authors
Feng L, Senchenkova SN, Tao J, Shashkov AS, Liu B, Shevelev SD, Reeves PR, Xu J, Knirel YA, Wang L
Title
Structural and genetic characterization of enterohemorrhagic Escherichia coli O145 O antigen and development of an O145 serogroup-specific PCR assay.
Journal
Reference
Authors
Westman EL, Preston A, Field RA, Lam JS
Title
Biosynthesis of a rare di-N-acetylated sugar in the lipopolysaccharides of both Pseudomonas aeruginosa and Bordetella pertussis occurs via an identical scheme despite different gene clusters.
Journal
Reference
Authors
King JD, Kocincova D, Westman EL, Lam JS
Title
Review: Lipopolysaccharide biosynthesis in Pseudomonas aeruginosa.
Journal
Reference
Authors
Perepelov AV, Liu B, Senchenkova SN, Shashkov AS, Shevelev SD, Feng L, Wang L, Knirel YA
Title
Structure of the O-antigen and characterization of the O-antigen gene cluster of Escherichia coli O108 containing 5,7-diacetamido-3,5,7,9-tetradeoxy-L-glycero-D-galacto-non-2-ulosonic (8-epilegionaminic) acid.
Journal
Reference
Authors
Hwang S, Aronov A, Bar-Peled M
Title
The Biosynthesis of UDP-D-QuiNAc in Bacillus cereus ATCC 14579.
Journal
Reference
Authors
McDonald ND, DeMeester KE, Lewis AL, Grimes CL, Boyd EF
Title
Structural and functional characterization of a modified legionaminic acid involved in glycosylation of a bacterial lipopolysaccharide.
Journal
Related pathway
plu00520 Amino sugar and nucleotide sugar metabolism
KO pathway
LinkDB
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