KEGG   ORTHOLOGY: K00143
Entry
K00143                      KO                                     
Symbol
LYS2
Name
L-2-aminoadipate reductase [EC:1.2.1.95]
Pathway
map00300  Lysine biosynthesis
map01100  Metabolic pathways
map01110  Biosynthesis of secondary metabolites
map01230  Biosynthesis of amino acids
Module
M00030  Lysine biosynthesis, AAA pathway, 2-oxoglutarate => 2-aminoadipate => lysine
Reaction
R03098  L-2-aminoadipate-6-semialdehyde:NADP+ 6-oxidoreductase
R04390  L-2-aminoadipate-6-semialdehyde:NADP+ 6-oxidoreductase
R04863  L-2-aminoadipate-6-semialdehyde:NADP+ 6-oxidoreductase
R11679  (S)-2-amino-6-oxohexanoate:NADP+ oxidoreductase (ATP-forming)
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09105 Amino acid metabolism
   00300 Lysine biosynthesis
    K00143  LYS2; L-2-aminoadipate reductase
Enzymes [BR:ko01000]
 1. Oxidoreductases
  1.2  Acting on the aldehyde or oxo group of donors
   1.2.1  With NAD+ or NADP+ as acceptor
    1.2.1.95  L-2-aminoadipate reductase
     K00143  LYS2; L-2-aminoadipate reductase
Other DBs
COG: COG1020 COG3320
Genes
SCE: YBR115C(LYS2)
SEUB: DI49_1147
SPAO: SPAR_B02160
AGO: AGOS_ADL346W
ERC: Ecym_3457
KLA: KLLA0_B09218g
KMX: KLMA_80299(LYS2)
LTH: KLTH0F10384g
VPO: Kpol_1006p6
ZRO: ZYRO0C16566g
CGR: 2890361(GVI51_K07667)
NCS: NCAS_0I01040(NCAS0I01040)
NDI: NDAI_0A07970(NDAI0A07970)
TPF: TPHA_0A03520(TPHA0A03520)
TBL: TBLA_0H01500(TBLA0H01500)
TGB: HG536_0B02090
TDL: TDEL_0C05110(TDEL0C05110)
KAF: KAFR_0D02630(KAFR0D02630)
KNG: KNAG_0B01430(KNAG0B01430)
ZMK: HG535_0A02150
SLUD: SCDLUD_000584
DHA: DEHA2D07964g
PIC: PICST_68020(LYS2)
PGU: PGUG_04759
SPAA: SPAPADRAFT_145301(LYS2)
LEL: PVL30_001487(LYS2)
LBG: 92208092(LODBEIA_P28960)
CAL: CAALFM_C102820WA(LYS2)
CTP: CTRG_04682
COT: CORT_0B09490
CDU: CD36_02620
CTEN: 18249543(LYS2)
CLU: CLUG_04446
CLUS: A9F13_04g00583
CAUR: CJI96_0001808
ASAU: 88172377(PUMCH_001311)
PPA: PAS_chr1-4_0153
PKZ: C5L36_0B12020
BNN: FOA43_003074
BBRX: BRETT_005327
OPA: HPODL_02426
YLI: 2912394(YALI2_E00144g)
SLB: AWJ20_3018(LYS2)
NCR: NCU03010(lys-3)
NTE: NEUTE1DRAFT126732(NEUTE1DRAFT_126732)
SMP: 10810014(SMAC4_01210)
PAN: PODANSg09765
PBEL: QC761_105110(LYS2)
PPSD: QC762_105110(LYS2)
PPSP: QC763_105110(LYS2)
PPSA: QC764_105110(LYS2)
TTT: THITE_2122221
MTM: MYCTH_2295392
CTHR: CTHT_0055440
RTHE: 98128728(VTJ83DRAFT_728)
MGR: MGG_02611
PPEI: PpBr36_09833
PGRI: PgNI_10816
TMN: UCRPA7_7828
SSCK: SPSK_02958
FGR: FGSG_06041
FPU: FPSE_02931
FPOA: FPOAC1_009008
FVN: FVRRES_09626
FVR: FVEG_08245
FOX: FOXG_01867 FOXG_11115
NHE: NECHADRAFT_76435(NhLYS2) NECHADRAFT_81329
FFC: NCS54_00258900
FKR: NCS57_00254400 NCS57_00831400
FMU: J7337_002716
TRE: TRIREDRAFT_4117
TRR: M419DRAFT_67857
TATV: 25775215(TrAtP1_008831) 25780019(TrAtP1_009880)
TASP: 36607608(TrAFT101_002608) 36617108(TrAFT101_011006)
MAW: 19253986(LYS2_8)
MAJ: MAA_03758
MBRN: 26245502(lys2)
PCHM: VFPPC_00985
CMT: CCM_03390
AMUS: LMH87_002803
PLJ: 28891341(PLICBS_010574)
PTKZ: JDV02_004246(LYS2)
VAL: VDBG_05463
VDA: VDAG_07112
CFJ: CFIO01_05721
CLUP: CLUP02_04765
CHIG: CH63R_10366
CDET: 87943470(CDEST_06967)
SAPO: SAPIO_CDS6728 SAPIO_CDS9122
ELA: UCREL1_11578
PFY: PFICI_11756
SSL: SS1G_08185
BFU: BCIN_04g00140(Bclys2)
MBE: MBM_08979
PSCO: LY89DRAFT_784917
GLZ: GLAREA_03383
ANI: ANIA_05610
AFM: AFUA_4G11240
ACT: ACLA_050330
NFI: NFIA_105160
AOR: AO090003001097
ANG: An04g05420
AFV: AFLA_000572
ALUC: AKAW2_51512A(LYS2)
ACHE: ACHE_40446S(LYS2_2)
APUU: APUU_40975S(LYS2)
PCS: N7525_005598
PDP: PDIP_33720
POU: POX_a01534
TMF: EYB26_000537
TRG: TRUGW13939_04324
CIM: CIMG_01491
CPW: 9691197(LYS2)
URE: UREG_05106
ABE: ARB_02279
TVE: TRV_04974
PBL: PAAG_08023
PBN: PADG_08092
AJE: HCAG_09107
BGH: BDBG_06043
PNO: SNOG_07784
PTE: PTT_09387
PTRR: 6343897(PtrM4_108130)
BZE: COCCADRAFT_321
BSC: COCSADRAFT_105593(AAR)
BOR: COCMIDRAFT_912
AALT: CC77DRAFT_1016196
ADAC: 96089590(ACET3X_009268)
ARAB: EKO05_0006667
ZTR: MYCGRDRAFT_99148(LYS2)
PFJ: MYCFIDRAFT_46827
FFU: CLAFUR5_09844
CBET: CB0940_06181
BCOM: BAUCODRAFT_263156
NPA: UCRNP2_5911
TML: GSTUM_00010502001
SPO: 2542079(lys1)
SOM: SOMG_03047(lys1)
CNE: CNG00170
CNB: CNBG4570
CNG: CNAG_03588
CGI: CGB_G6500C
CDEU: CNBG_2041
CDEC: 89992202(IAS62_005432)
CTEG: 91991342(I308_104486)
CBAS: 91984750(I312_104705)
CDEP: 91085490(L203_101277)
KMG: 30168218(I203_105058)
KNE: 92180493(IAR55_003235)
KDJ: 28969947(I303_106229)
KBI: 30211391(I302_107272)
KPIN: 30172276(I206_105184)
KSH: 87957850(IL334_005720)
KER: 91098860(V865_000056)
TMS: TREMEDRAFT_44814
TASA: A1Q1_01219
CCAC: CcaHIS019_0110290(LYS2)
TVS: TRAVEDRAFT_171224
DSQ: DICSQDRAFT_101950
PCO: PHACADRAFT_118411
SHS: STEHIDRAFT_85460
HIR: HETIRDRAFT_66316(lys2)
PSQ: PUNSTDRAFT_102428
ADL: AURDEDRAFT_110234
FME: FOMMEDRAFT_118412
GTR: GLOTRDRAFT_78681
RSX: RhiXN_07144
LBC: LACBIDRAFT_184376(LbLYS1)
CCI: CC1G_15694
ABP: AGABI1DRAFT79561(AGABI1DRAFT_79561)
ABV: AGABI2DRAFT208996(AGABI2DRAFT_208996)
PCUB: JR316_0010008
MRR: Moror_9060
MORE: E1B28_002534
SCM: SCHCO_02637370(SCHCODRAFT_02637370)
CPUT: CONPUDRAFT_168209
SLA: SERLADRAFT_359475
WSE: WALSEDRAFT_22743
WIC: J056_002659
UMA: UMAG_01697
PFP: PFL1_03572
SGRA: EX895_003775
MGL: MGL_0235
MRT: MRET_1079
MSYM: MSY001_3374
PGR: PGTG_07683
PTRC: PtA15_4A375
PSTR: Pst134EA_024066
MLR: MELLADRAFT_54227
RIE: OCT59_014513
SRE: PTSG_11594
ACAN: ACA1_063720
NGR: NAEGRDRAFT_77825
 » show all
Reference
PMID:10320345
  Authors
Ehmann DE, Gehring AM, Walsh CT
  Title
Lysine biosynthesis in Saccharomyces cerevisiae: mechanism of alpha-aminoadipate reductase (Lys2) involves posttranslational phosphopantetheinylation by Lys5.
  Journal
Biochemistry 38:6171-7 (1999)
DOI:10.1021/bi9829940
  Sequence
[sce:YBR115C]
LinkDB

KEGG   ENZYME: 1.2.1.95
Entry
EC 1.2.1.95                 Enzyme                                 
Name
L-2-aminoadipate reductase;
LYS2;
alpha-aminoadipate reductase
Class
Oxidoreductases;
Acting on the aldehyde or oxo group of donors;
With NAD+ or NADP+ as acceptor
Sysname
(S)-2-amino-6-oxohexanoate:NADP+ oxidoreductase (ATP-forming)
Reaction(IUBMB)
(S)-2-amino-6-oxohexanoate + NADP+ + AMP + diphosphate = L-2-aminoadipate + NADPH + H+ + ATP (overall reaction) [RN:R11679];
(1a) L-2-aminoadipyl-[LYS2 peptidyl-carrier-protein] + AMP + diphosphate = L-2-aminoadipate + holo-[LYS2 peptidyl-carrier-protein] + ATP;
(1b) (S)-2-amino-6-oxohexanoate + holo-[LYS2 peptidyl-carrier-protein] + NADP+ = L-2-aminoadipyl-[LYS2 peptidyl-carrier-protein] + NADPH + H+
Reaction(KEGG)
R11679;
(other) R03098 R04390 R04863
Substrate
(S)-2-amino-6-oxohexanoate [CPD:C04076];
NADP+ [CPD:C00006];
AMP [CPD:C00020];
diphosphate [CPD:C00013];
L-2-aminoadipyl-[LYS2 peptidyl-carrier-protein];
holo-[LYS2 peptidyl-carrier-protein]
Product
L-2-aminoadipate [CPD:C00956];
NADPH [CPD:C00005];
H+ [CPD:C00080];
ATP [CPD:C00002];
holo-[LYS2 peptidyl-carrier-protein];
L-2-aminoadipyl-[LYS2 peptidyl-carrier-protein]
Comment
This enzyme, characterized from the yeast Saccharomyces cerevisiae, catalyses the reduction of L-2-aminoadipate to (S)-2-amino-6-oxohexanoate during L-lysine biosynthesis. An adenylation domain activates the substrate at the expense of ATP hydrolysis, and forms L-2-aminoadipate adenylate, which is attached to a peptidyl-carrier protein (PCP) domain. Binding of NADPH results in reductive cleavage of the acyl-S-enzyme intermediate, releasing (S)-2-amino-6-oxohexanoate. Different from EC 1.2.1.31, L-aminoadipate-semialdehyde dehydrogenase, which catalyses a similar transformation in the opposite direction without ATP hydrolysis.
History
EC 1.2.1.95 created 2015
Pathway
ec00300  Lysine biosynthesis
ec01100  Metabolic pathways
ec01110  Biosynthesis of secondary metabolites
Orthology
K00143  L-2-aminoadipate reductase
Genes
SCE: YBR115C(LYS2)
SEUB: DI49_1147
SPAO: SPAR_B02160
AGO: AGOS_ADL346W
ERC: Ecym_3457
KLA: KLLA0_B09218g
KMX: KLMA_80299(LYS2)
LTH: KLTH0F10384g
VPO: Kpol_1006p6
ZRO: ZYRO0C16566g
CGR: 2890361(GVI51_K07667)
NCS: NCAS_0I01040(NCAS0I01040)
NDI: NDAI_0A07970(NDAI0A07970)
TPF: TPHA_0A03520(TPHA0A03520)
TBL: TBLA_0H01500(TBLA0H01500)
TGB: HG536_0B02090
TDL: TDEL_0C05110(TDEL0C05110)
KAF: KAFR_0D02630(KAFR0D02630)
KNG: KNAG_0B01430(KNAG0B01430)
ZMK: HG535_0A02150
SLUD: SCDLUD_000584
DHA: DEHA2D07964g
PIC: PICST_68020(LYS2)
PGU: PGUG_04759
SPAA: SPAPADRAFT_145301(LYS2)
LEL: PVL30_001487(LYS2)
LBG: 92208092(LODBEIA_P28960)
CAL: CAALFM_C102820WA(LYS2)
CTP: CTRG_04682
COT: CORT_0B09490
CDU: CD36_02620
CTEN: 18249543(LYS2)
CLU: CLUG_04446
CLUS: A9F13_04g00583
CAUR: CJI96_0001808
ASAU: 88172377(PUMCH_001311)
PPA: PAS_chr1-4_0153
PKZ: C5L36_0B12020
BNN: FOA43_003074
BBRX: BRETT_005327
OPA: HPODL_02426
YLI: 2912394(YALI2_E00144g)
SLB: AWJ20_3018(LYS2)
NCR: NCU03010(lys-3)
NTE: NEUTE1DRAFT126732(NEUTE1DRAFT_126732)
SMP: 10810014(SMAC4_01210)
PAN: PODANSg09765
PBEL: QC761_105110(LYS2)
PPSD: QC762_105110(LYS2)
PPSP: QC763_105110(LYS2)
PPSA: QC764_105110(LYS2)
TTT: THITE_2122221
MTM: MYCTH_2295392
CTHR: CTHT_0055440
RTHE: 98128728(VTJ83DRAFT_728)
MGR: MGG_02611
PPEI: PpBr36_09833
PGRI: PgNI_10816
TMN: UCRPA7_7828
SSCK: SPSK_02958
FGR: FGSG_06041
FPU: FPSE_02931
FPOA: FPOAC1_009008
FVN: FVRRES_09626
FVR: FVEG_08245
FOX: FOXG_01867 FOXG_11115
NHE: NECHADRAFT_76435(NhLYS2) NECHADRAFT_81329
FFC: NCS54_00258900
FKR: NCS57_00254400 NCS57_00831400
FMU: J7337_002716
TRE: TRIREDRAFT_4117
TRR: M419DRAFT_67857
TATV: 25775215(TrAtP1_008831) 25780019(TrAtP1_009880)
TASP: 36607608(TrAFT101_002608) 36617108(TrAFT101_011006)
MAW: 19253986(LYS2_8)
MAJ: MAA_03758
MBRN: 26245502(lys2)
PCHM: VFPPC_00985
CMT: CCM_03390
AMUS: LMH87_002803
PLJ: 28891341(PLICBS_010574)
PTKZ: JDV02_004246(LYS2)
VAL: VDBG_05463
VDA: VDAG_07112
CFJ: CFIO01_05721
CLUP: CLUP02_04765
CHIG: CH63R_10366
CDET: 87943470(CDEST_06967)
SAPO: SAPIO_CDS6728 SAPIO_CDS9122
ELA: UCREL1_11578
PFY: PFICI_11756
SSL: SS1G_08185
BFU: BCIN_04g00140(Bclys2)
MBE: MBM_08979
PSCO: LY89DRAFT_784917
GLZ: GLAREA_03383
ANI: ANIA_05610
AFM: AFUA_4G11240
ACT: ACLA_050330
NFI: NFIA_105160
AOR: AO090003001097
ANG: An04g05420
AFV: AFLA_000572
ALUC: AKAW2_51512A(LYS2)
ACHE: ACHE_40446S(LYS2_2)
APUU: APUU_40975S(LYS2)
PCS: N7525_005598
PDP: PDIP_33720
POU: POX_a01534
TMF: EYB26_000537
TRG: TRUGW13939_04324
CIM: CIMG_01491
CPW: 9691197(LYS2)
URE: UREG_05106
ABE: ARB_02279
TVE: TRV_04974
PBL: PAAG_08023
PBN: PADG_08092
AJE: HCAG_09107
BGH: BDBG_06043
PNO: SNOG_07784
PTE: PTT_09387
PTRR: 6343897(PtrM4_108130)
BZE: COCCADRAFT_321
BSC: COCSADRAFT_105593(AAR)
BOR: COCMIDRAFT_912
AALT: CC77DRAFT_1016196
ADAC: 96089590(ACET3X_009268)
ARAB: EKO05_0006667
ZTR: MYCGRDRAFT_99148(LYS2)
PFJ: MYCFIDRAFT_46827
FFU: CLAFUR5_09844
CBET: CB0940_06181
BCOM: BAUCODRAFT_263156
NPA: UCRNP2_5911
TML: GSTUM_00010502001
SPO: 2542079(lys1)
SOM: SOMG_03047(lys1)
CNE: CNG00170
CNB: CNBG4570
CNG: CNAG_03588
CGI: CGB_G6500C
CDEU: CNBG_2041
CDEC: 89992202(IAS62_005432)
CTEG: 91991342(I308_104486)
CBAS: 91984750(I312_104705)
CDEP: 91085490(L203_101277)
KMG: 30168218(I203_105058)
KNE: 92180493(IAR55_003235)
KDJ: 28969947(I303_106229)
KBI: 30211391(I302_107272)
KPIN: 30172276(I206_105184)
KSH: 87957850(IL334_005720)
KER: 91098860(V865_000056)
TMS: TREMEDRAFT_44814
TASA: A1Q1_01219
CCAC: CcaHIS019_0110290(LYS2)
TVS: TRAVEDRAFT_171224
DSQ: DICSQDRAFT_101950
PCO: PHACADRAFT_118411
SHS: STEHIDRAFT_85460
HIR: HETIRDRAFT_66316(lys2)
PSQ: PUNSTDRAFT_102428
ADL: AURDEDRAFT_110234
FME: FOMMEDRAFT_118412
GTR: GLOTRDRAFT_78681
RSX: RhiXN_07144
LBC: LACBIDRAFT_184376(LbLYS1)
CCI: CC1G_15694
ABP: AGABI1DRAFT79561(AGABI1DRAFT_79561)
ABV: AGABI2DRAFT208996(AGABI2DRAFT_208996)
PCUB: JR316_0010008
MRR: Moror_9060
MORE: E1B28_002534
SCM: SCHCO_02637370(SCHCODRAFT_02637370)
CPUT: CONPUDRAFT_168209
SLA: SERLADRAFT_359475
WSE: WALSEDRAFT_22743
WIC: J056_002659
UMA: UMAG_01697
PFP: PFL1_03572
SGRA: EX895_003775
MGL: MGL_0235
MRT: MRET_1079
MSYM: MSY001_3374
PGR: PGTG_07683
PTRC: PtA15_4A375
PSTR: Pst134EA_024066
MLR: MELLADRAFT_54227
RIE: OCT59_014513
SRE: PTSG_11594
ACAN: ACA1_063720
NGR: NAEGRDRAFT_77825
 » show all
Reference
1  [PMID:10320345]
  Authors
Ehmann DE, Gehring AM, Walsh CT
  Title
Lysine biosynthesis in Saccharomyces cerevisiae: mechanism of alpha-aminoadipate reductase (Lys2) involves posttranslational phosphopantetheinylation by Lys5.
  Journal
Biochemistry 38:6171-7 (1999)
DOI:10.1021/bi9829940
  Sequence
[sce:YBR115C]
Other DBs
ExplorEnz - The Enzyme Database: 1.2.1.95
IUBMB Enzyme Nomenclature: 1.2.1.95
ExPASy - ENZYME nomenclature database: 1.2.1.95
BRENDA, the Enzyme Database: 1.2.1.95
LinkDB

KEGG   REACTION: R04863
Entry
R04863                      Reaction                               
Name
L-2-aminoadipate-6-semialdehyde:NADP+ 6-oxidoreductase
Definition
L-2-Aminoadipate adenylate + Holo-Lys2 <=> alpha-Aminoadipoyl-S-acyl enzyme + AMP
Equation
C05560 + C11482 <=> C05535 + C00020
Comment
the second step of three-step reaction (see R03098+R04863+R04390)
for reverse direction, one-step reaction (see R03103)
Reaction class
RC00039  C05535_C11482
RC00070  C00020_C05560
RC02816  C05535_C05560
Enzyme
1.2.1.95
Pathway
rn00300  Lysine biosynthesis
rn01100  Metabolic pathways
rn01110  Biosynthesis of secondary metabolites
Module
M00030  Lysine biosynthesis, AAA pathway, 2-oxoglutarate => 2-aminoadipate => lysine
Brite
Enzymatic reactions [BR:br08201]
 1. Oxidoreductase reactions
  1.2  Acting on the aldehyde or oxo group of donors
   1.2.1  With NAD+ or NADP+ as acceptor
    1.2.1.95
     R04863  L-2-Aminoadipate adenylate + Holo-Lys2 <=> alpha-Aminoadipoyl-S-acyl enzyme + AMP
Orthology
K00143  L-2-aminoadipate reductase [EC:1.2.1.95]
LinkDB

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