KEGG   ORTHOLOGY: K00143
Entry
K00143                      KO                                     

Name
LYS2
Definition
L-2-aminoadipate reductase [EC:1.2.1.95]
Pathway
ko00300  Lysine biosynthesis
ko01100  Metabolic pathways
ko01110  Biosynthesis of secondary metabolites
ko01230  Biosynthesis of amino acids
Module
M00030  Lysine biosynthesis, AAA pathway, 2-oxoglutarate => 2-aminoadipate => lysine
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09105 Amino acid metabolism
   00300 Lysine biosynthesis
    K00143  LYS2; L-2-aminoadipate reductase
Enzymes [BR:ko01000]
 1. Oxidoreductases
  1.2  Acting on the aldehyde or oxo group of donors
   1.2.1  With NAD+ or NADP+ as acceptor
    1.2.1.95  L-2-aminoadipate reductase
     K00143  LYS2; L-2-aminoadipate reductase
Other DBs
RN: R03098 R04390 R04863 R11679
COG: COG1020 COG3320
Genes
QSU: 112018202 112040294
SCE: YBR115C(LYS2)
AGO: AGOS_ADL346W
ERC: Ecym_3457
KLA: KLLA0_B09218g
KMX: KLMA_80299(LYS2)
LTH: KLTH0F10384g
VPO: Kpol_1006p6
ZRO: ZYRO0C16566g
CGR: CAGL0K07788g
NCS: NCAS_0I01040(NCAS0I01040)
NDI: NDAI_0A07970(NDAI0A07970)
TPF: TPHA_0A03520(TPHA0A03520)
TBL: TBLA_0H01500(TBLA0H01500)
TDL: TDEL_0C05110(TDEL0C05110)
KAF: KAFR_0D02630(KAFR0D02630)
PPA: PAS_chr1-4_0153
DHA: DEHA2D07964g
PIC: PICST_68020(LYS2)
PGU: PGUG_04759
SPAA: SPAPADRAFT_145301(LYS2)
LEL: LELG_01517
CAL: CAALFM_C102820WA(LYS2)
CTP: CTRG_04682
COT: CORT_0B09490
CDU: CD36_02620
CTEN: CANTEDRAFT_131638
YLI: YALI0E06457g
CLU: CLUG_04446
CLUS: A9F13_04g00583
CAUR: CJI97_003346
SLB: AWJ20_3018(LYS2)
PKZ: C5L36_0B12020
NCR: NCU03010(lys-3)
NTE: NEUTE1DRAFT126732(NEUTE1DRAFT_126732)
SMP: SMAC_01210
PAN: PODANSg09765
TTT: THITE_2122221
MTM: MYCTH_2295392
CTHR: CTHT_0055440
MGR: MGG_02611
TMN: UCRPA7_7828
SSCK: SPSK_02958
FGR: FGSG_06041
FPU: FPSE_02931
FVR: FVEG_08245
FOX: FOXG_01867 FOXG_11115
NHE: NECHADRAFT_76435(NhLYS2) NECHADRAFT_81329
TRE: TRIREDRAFT_4117
TRR: M419DRAFT_67857
MAW: MAC_09675
MAJ: MAA_03758
CMT: CCM_03390
PLJ: VFPFJ_09219
VAL: VDBG_05463
VDA: VDAG_07112
CFJ: CFIO01_05721
SAPO: SAPIO_CDS6728 SAPIO_CDS9122
ELA: UCREL1_11578
PFY: PFICI_11756
SSL: SS1G_08185
BFU: BCIN_04g00140(Bclys2)
MBE: MBM_08979
PSCO: LY89DRAFT_784917
GLZ: GLAREA_03383
ANI: AN5610.2
AFM: AFUA_4G11240
ACT: ACLA_050330
NFI: NFIA_105160
AOR: AO090003001097
ANG: ANI_1_1902184(An04g05420)
AFV: AFLA_027230
PCS: Pc22g06310(Lys2)
PDP: PDIP_33720
TMF: PMAA_020860
TRG: TRUGW13939_04324
CIM: CIMG_01491
CPW: CPC735_049520
PBL: PAAG_08023
PBN: PADG_08092
URE: UREG_05106
ABE: ARB_02279
TVE: TRV_04974
AJE: HCAG_09107
BGH: BDBG_06043
PNO: SNOG_07784
PTE: PTT_09387
BZE: COCCADRAFT_321
BSC: COCSADRAFT_105593(AAR)
BOR: COCMIDRAFT_912
AALT: CC77DRAFT_1016196
ZTR: MYCGRDRAFT_99148(LYS2)
PFJ: MYCFIDRAFT_46827
BCOM: BAUCODRAFT_263156
NPA: UCRNP2_5911
TML: GSTUM_00010502001
SPO: SPAP7G5.04c(lys1)
CNE: CNG00170
CNB: CNBG4570
CGI: CGB_G6500C
TMS: TREMEDRAFT_44814
TASA: A1Q1_01219
TVS: TRAVEDRAFT_171224
DSQ: DICSQDRAFT_101950
PCO: PHACADRAFT_118411
SHS: STEHIDRAFT_85460
HIR: HETIRDRAFT_66316(lys2)
PSQ: PUNSTDRAFT_102428
ADL: AURDEDRAFT_110234
FME: FOMMEDRAFT_118412
GTR: GLOTRDRAFT_78681
LBC: LACBIDRAFT_184376(LbLYS1)
MRR: Moror_9060
CCI: CC1G_15694
SCM: SCHCODRAFT_59997
ABP: AGABI1DRAFT79561(AGABI1DRAFT_79561)
ABV: AGABI2DRAFT208996(AGABI2DRAFT_208996)
CPUT: CONPUDRAFT_168209
SLA: SERLADRAFT_359475
WSE: WALSEDRAFT_22743
WIC: J056_002659
UMA: UMAG_01697
PFP: PFL1_03572
MGL: MGL_0235
MRT: MRET_1079
MSYM: MSY001_3374
PGR: PGTG_07683
MLR: MELLADRAFT_54227
SRE: PTSG_11594
ACAN: ACA1_063720
NGR: NAEGRDRAFT_77825
 » show all
Reference
  Authors
Ehmann DE, Gehring AM, Walsh CT
  Title
Lysine biosynthesis in Saccharomyces cerevisiae: mechanism of alpha-aminoadipate reductase (Lys2) involves posttranslational phosphopantetheinylation by Lys5.
  Journal
Biochemistry 38:6171-7 (1999)
DOI:10.1021/bi9829940
  Sequence
[sce:YBR115C]
LinkDB

KEGG   ENZYME: 1.2.1.95
Entry
EC 1.2.1.95                 Enzyme                                 

Name
L-2-aminoadipate reductase;
LYS2;
alpha-aminoadipate reductase
Class
Oxidoreductases;
Acting on the aldehyde or oxo group of donors;
With NAD+ or NADP+ as acceptor
Sysname
(S)-2-amino-6-oxohexanoate:NADP+ oxidoreductase (ATP-forming)
Reaction(IUBMB)
(S)-2-amino-6-oxohexanoate + NADP+ + AMP + diphosphate = L-2-aminoadipate + NADPH + H+ + ATP (overall reaction) [RN:R11679];
(1a) L-2-aminoadipyl-[LYS2 peptidyl-carrier-protein] + AMP + diphosphate = L-2-aminoadipate + holo-[LYS2 peptidyl-carrier-protein] + ATP;
(1b) (S)-2-amino-6-oxohexanoate + holo-[LYS2 peptidyl-carrier-protein] + NADP+ = L-2-aminoadipyl-[LYS2 peptidyl-carrier-protein] + NADPH + H+
Reaction(KEGG)
R11679;
(other) R03098 R04390 R04863
Substrate
(S)-2-amino-6-oxohexanoate [CPD:C04076];
NADP+ [CPD:C00006];
AMP [CPD:C00020];
diphosphate [CPD:C00013];
L-2-aminoadipyl-[LYS2 peptidyl-carrier-protein];
holo-[LYS2 peptidyl-carrier-protein]
Product
L-2-aminoadipate [CPD:C00956];
NADPH [CPD:C00005];
H+ [CPD:C00080];
ATP [CPD:C00002];
holo-[LYS2 peptidyl-carrier-protein];
L-2-aminoadipyl-[LYS2 peptidyl-carrier-protein]
Comment
This enzyme, characterized from the yeast Saccharomyces cerevisiae, catalyses the reduction of L-2-aminoadipate to (S)-2-amino-6-oxohexanoate during L-lysine biosynthesis. An adenylation domain activates the substrate at the expense of ATP hydrolysis, and forms L-2-aminoadipate adenylate, which is attached to a peptidyl-carrier protein (PCP) domain. Binding of NADPH results in reductive cleavage of the acyl-S-enzyme intermediate, releasing (S)-2-amino-6-oxohexanoate. Different from EC 1.2.1.31, L-aminoadipate-semialdehyde dehydrogenase, which catalyses a similar transformation in the opposite direction without ATP hydrolysis.
History
EC 1.2.1.95 created 2015
Pathway
ec00300  Lysine biosynthesis
ec01100  Metabolic pathways
ec01110  Biosynthesis of secondary metabolites
Orthology
K00143  L-2-aminoadipate reductase
Genes
QSU: 112018202 112040294
SCE: YBR115C(LYS2)
AGO: AGOS_ADL346W
ERC: Ecym_3457
KLA: KLLA0_B09218g
KMX: KLMA_80299(LYS2)
LTH: KLTH0F10384g
VPO: Kpol_1006p6
ZRO: ZYRO0C16566g
CGR: CAGL0K07788g
NCS: NCAS_0I01040(NCAS0I01040)
NDI: NDAI_0A07970(NDAI0A07970)
TPF: TPHA_0A03520(TPHA0A03520)
TBL: TBLA_0H01500(TBLA0H01500)
TDL: TDEL_0C05110(TDEL0C05110)
KAF: KAFR_0D02630(KAFR0D02630)
PPA: PAS_chr1-4_0153
DHA: DEHA2D07964g
PIC: PICST_68020(LYS2)
PGU: PGUG_04759
SPAA: SPAPADRAFT_145301(LYS2)
LEL: LELG_01517
CAL: CAALFM_C102820WA(LYS2)
CTP: CTRG_04682
COT: CORT_0B09490
CDU: CD36_02620
CTEN: CANTEDRAFT_131638
YLI: YALI0E06457g
CLU: CLUG_04446
CLUS: A9F13_04g00583
CAUR: CJI97_003346
SLB: AWJ20_3018(LYS2)
PKZ: C5L36_0B12020
NCR: NCU03010(lys-3)
NTE: NEUTE1DRAFT126732(NEUTE1DRAFT_126732)
SMP: SMAC_01210
PAN: PODANSg09765
TTT: THITE_2122221
MTM: MYCTH_2295392
CTHR: CTHT_0055440
MGR: MGG_02611
TMN: UCRPA7_7828
SSCK: SPSK_02958
FGR: FGSG_06041
FPU: FPSE_02931
FVR: FVEG_08245
FOX: FOXG_01867 FOXG_11115
NHE: NECHADRAFT_76435(NhLYS2) NECHADRAFT_81329
TRE: TRIREDRAFT_4117
TRR: M419DRAFT_67857
MAW: MAC_09675
MAJ: MAA_03758
CMT: CCM_03390
PLJ: VFPFJ_09219
VAL: VDBG_05463
VDA: VDAG_07112
CFJ: CFIO01_05721
SAPO: SAPIO_CDS6728 SAPIO_CDS9122
ELA: UCREL1_11578
PFY: PFICI_11756
SSL: SS1G_08185
BFU: BCIN_04g00140(Bclys2)
MBE: MBM_08979
PSCO: LY89DRAFT_784917
GLZ: GLAREA_03383
ANI: AN5610.2
AFM: AFUA_4G11240
ACT: ACLA_050330
NFI: NFIA_105160
AOR: AO090003001097
ANG: ANI_1_1902184(An04g05420)
AFV: AFLA_027230
PCS: Pc22g06310(Lys2)
PDP: PDIP_33720
TMF: PMAA_020860
TRG: TRUGW13939_04324
CIM: CIMG_01491
CPW: CPC735_049520
PBL: PAAG_08023
PBN: PADG_08092
URE: UREG_05106
ABE: ARB_02279
TVE: TRV_04974
AJE: HCAG_09107
BGH: BDBG_06043
PNO: SNOG_07784
PTE: PTT_09387
BZE: COCCADRAFT_321
BSC: COCSADRAFT_105593(AAR)
BOR: COCMIDRAFT_912
AALT: CC77DRAFT_1016196
ZTR: MYCGRDRAFT_99148(LYS2)
PFJ: MYCFIDRAFT_46827
BCOM: BAUCODRAFT_263156
NPA: UCRNP2_5911
TML: GSTUM_00010502001
SPO: SPAP7G5.04c(lys1)
CNE: CNG00170
CNB: CNBG4570
CGI: CGB_G6500C
TMS: TREMEDRAFT_44814
TASA: A1Q1_01219
TVS: TRAVEDRAFT_171224
DSQ: DICSQDRAFT_101950
PCO: PHACADRAFT_118411
SHS: STEHIDRAFT_85460
HIR: HETIRDRAFT_66316(lys2)
PSQ: PUNSTDRAFT_102428
ADL: AURDEDRAFT_110234
FME: FOMMEDRAFT_118412
GTR: GLOTRDRAFT_78681
LBC: LACBIDRAFT_184376(LbLYS1)
MRR: Moror_9060
CCI: CC1G_15694
SCM: SCHCODRAFT_59997
ABP: AGABI1DRAFT79561(AGABI1DRAFT_79561)
ABV: AGABI2DRAFT208996(AGABI2DRAFT_208996)
CPUT: CONPUDRAFT_168209
SLA: SERLADRAFT_359475
WSE: WALSEDRAFT_22743
WIC: J056_002659
UMA: UMAG_01697
PFP: PFL1_03572
MGL: MGL_0235
MRT: MRET_1079
MSYM: MSY001_3374
PGR: PGTG_07683
MLR: MELLADRAFT_54227
SRE: PTSG_11594
ACAN: ACA1_063720
NGR: NAEGRDRAFT_77825
 » show all
Reference
1  [PMID:10320345]
  Authors
Ehmann DE, Gehring AM, Walsh CT
  Title
Lysine biosynthesis in Saccharomyces cerevisiae: mechanism of alpha-aminoadipate reductase (Lys2) involves posttranslational phosphopantetheinylation by Lys5.
  Journal
Biochemistry 38:6171-7 (1999)
DOI:10.1021/bi9829940
  Sequence
[sce:YBR115C]
Other DBs
ExplorEnz - The Enzyme Database: 1.2.1.95
IUBMB Enzyme Nomenclature: 1.2.1.95
ExPASy - ENZYME nomenclature database: 1.2.1.95
BRENDA, the Enzyme Database: 1.2.1.95
LinkDB

KEGG   REACTION: R04863
Entry
R04863                      Reaction                               

Name
L-2-aminoadipate-6-semialdehyde:NADP+ 6-oxidoreductase
Definition
L-2-Aminoadipate adenylate + Holo-Lys2 <=> alpha-Aminoadipoyl-S-acyl enzyme + AMP
Equation
Comment
The second step of three-step reaction (R03098 + R04863 + R04390)
For reverse direction, one-step reaction (see R03103)
Reaction class
RC00039  C05535_C11482
RC00070  C00020_C05560
RC02816  C05535_C05560
Enzyme
Pathway
rn00300  Lysine biosynthesis
rn01100  Metabolic pathways
rn01110  Biosynthesis of secondary metabolites
Module
M00030  Lysine biosynthesis, AAA pathway, 2-oxoglutarate => 2-aminoadipate => lysine
Orthology
K00143  L-2-aminoadipate reductase [EC:1.2.1.95]
LinkDB

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