KEGG   ORTHOLOGY: K00264
Entry
K00264                      KO                                     
Symbol
GLT1
Name
glutamate synthase (NADH) [EC:1.4.1.14]
Pathway
map00250  Alanine, aspartate and glutamate metabolism
map00910  Nitrogen metabolism
map01100  Metabolic pathways
map01110  Biosynthesis of secondary metabolites
map01120  Microbial metabolism in diverse environments
map01230  Biosynthesis of amino acids
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09102 Energy metabolism
   00910 Nitrogen metabolism
    K00264  GLT1; glutamate synthase (NADH)
  09105 Amino acid metabolism
   00250 Alanine, aspartate and glutamate metabolism
    K00264  GLT1; glutamate synthase (NADH)
Enzymes [BR:ko01000]
 1. Oxidoreductases
  1.4  Acting on the CH-NH2 group of donors
   1.4.1  With NAD+ or NADP+ as acceptor
    1.4.1.14  glutamate synthase (NADH)
     K00264  GLT1; glutamate synthase (NADH)
Other DBs
RN: R00093
COG: COG0067 COG0069 COG0070 COG0493
GO: 0016040
Genes
PTEH: 111534102 113223760 113223761
BFO: 118423229
BBEL: 109473320
SPU: 754163
DME: Dmel_CG9674(CG9674)
DER: 6543630
DSE: 6606013
DSI: Dsimw501_GD12441(Dsim_GD12441)
DYA: Dyak_GE22188
DAN: 6493372
DSR: 110190291
DPE: 6601003
DMN: 108152363
DWI: 6645095
DGR: 6557958
DAZ: 108613420
DNV: 108650790
DHE: 111605684
DVI: 6622079
CCAT: 101448290
BOD: 106620726
MDE: 101893373
SCAC: 106092120
ACOZ: 120951886
AARA: 120895665
AAG: 5565246
AALB: 109410517
CPII: 120430753
CNS: 116345940
AME: 413372
ACER: 108004089
ALAB: 122714169
BIM: 100743936
BBIF: 117208172
BVK: 117235398
BVAN: 117154250
BTER: 100648506
BPYO: 122565625
CCAL: 108629149
OBB: 114879469
MGEN: 117218009
NMEA: 116432027
CGIG: 122398534
SOC: 105197574
MPHA: 105838501
AEC: 105149802
ACEP: 105627138
PBAR: 105424147
VEM: 105559493
HST: 105181045
DQU: 106749864
CFO: 105259420
FEX: 115245214
LHU: 105677426
PGC: 109859877
OBO: 105286430
PCF: 106785427
PFUC: 122514263
VPS: 122634919
NVI: 100122109
CSOL: 105368803
TPRE: 106656707
MDL: 103570096
CGLO: 123272834
FAS: 105270599
DAM: 107043748
AGIF: 122853354
CCIN: 107270167
TCA: 658584
DPA: 109543624
SOY: 115886449
ATD: 109601527
CSET: 123312571
AGB: 108917077
LDC: 111508893
APLN: 108732952
PPYR: 116171129
OTU: 111413251
MSEX: 115452078
BANY: 112047329
PPOT: 106100510
PXU: 106125288
PRAP: 110993503
ZCE: 119831402
OFU: 114358173
API: 100158883
DNX: 107164646
AGS: 114126871
RMD: 113555018
BTAB: 109029618
DCI: 103524615
CLEC: 106667559
HHAL: 106690746
NLU: 111052344
FOC: 113206005
ZNE: 110836732
CSEC: 111865708
DMK: 116920522
PVM: 113799962
PJA: 122255183
PCHN: 125045218
HAME: 121879794
PCLA: 123755771
PTRU: 123520672
HAZT: 108678918
EAF: 111704323
DSV: 119461673
RSAN: 119395866
RMP: 119174150
VDE: 111247835
VJA: 111272506
DFR: 124499735
CEL: CELE_W07E11.1(W07E11.1)
CBR: CBG_01975
BGT: 106056462
GAE: 121371892
HRF: 124116337
HRJ: 124282667
CRG: 105337796
PMAX: 117327627
MMER: 123552518
OSN: 115223129
XEN: 124443157
ATH: AT5G53460(GLT1)
ALY: 9300320
CRB: 17876423
BRP: 103857079
BOE: 106332590
RSZ: 108845736
THJ: 104811925
CPAP: 110823143
CIT: 102620225
PVY: 116133280
TCC: 18605743
GRA: 105765457
GAB: 108479092
EGR: 104437859
GMX: 100815931
GSJ: 114410308
VRA: 106764633
VAR: 108331410
VUN: 114194130
CCAJ: 109814577
APRC: 113850812
CAM: 101509615
ADU: 107495014
AIP: 107605257
LANG: 109326556
FVE: 101309397
RCN: 112183464
PPER: 18785174
PMUM: 103333633
PAVI: 110767133
PDUL: 117619118
MDM: 103443818
ZJU: 107414598
MNT: 21390250
CSV: 101213224
CMO: 103485701
BHJ: 120081937
MCHA: 111011404
CMAX: 111482791
CMOS: 111430634
CPEP: 111810135
RCU: 8274353
JCU: 105648339
QLO: 115959755
VVI: 100246868
VRI: 117933639
SLY: 101254281
SPEN: 107013700
SOT: 102580516
CANN: 107855963
NSY: 104244568
NAU: 109213942
INI: 109183410
ITR: 116012569
SIND: 105160371
OEU: 111405628
EGT: 105971643
ECAD: 122586618
LSV: 111918005
BVG: 104888263
SOE: 110783090
NCOL: 116266917
DOSA: Os05t0555600-01(Os05g0555600)
ATS: 109760356
SBI: 8068026
SITA: 101785947
SVS: 117857834
PHAI: 112894413
DCT: 110110468
PEQ: 110020245
AOF: 109840747
ATR: 18442390
PPP: 112283025
APRO: F751_6615
MIS: MICPUN_105473(GLT)
SCE: YDL171C(GLT1)
ERC: Ecym_4713
KMX: KLMA_50304(GLT1)
NCS: NCAS_0A14190(NCAS0A14190)
NDI: NDAI_0A01860(NDAI0A01860)
TPF: TPHA_0F03070(TPHA0F03070)
TDL: TDEL_0C01870(TDEL0C01870)
PIC: PICST_87428(GLT1)
SPAA: SPAPADRAFT_141734(GLT1)
CAL: CAALFM_C106550WA(GLT1)
BNN: FOA43_002177(GLT1)
NCR: NCU01744(en(am)-2)
NTE: NEUTE1DRAFT65142(NEUTE1DRAFT_65142)
MGR: MGG_07187
SSCK: SPSK_08269
TRE: TRIREDRAFT_122287(glt1)
MAW: MAC_00032
MAJ: MAA_01819
CMT: CCM_03495
BFU: BCIN_15g02460(Bcglt1)
MBE: MBM_04374
ANI: AN5134.2
ANG: ANI_1_1272064(An07g09920)
ALUC: AKAW2_11294S(GLT1)
APUU: APUU_41225A(GLT1)
PBL: PAAG_12483(PAAG_07998)
ABE: ARB_04215
TVE: TRV_01094
PTE: PTT_08692
SPO: SPAPB1E7.07(glt1)
CNE: CNJ02910
CNB: CNBJ0620
TASA: A1Q1_06977
ABP: AGABI1DRAFT104661(AGABI1DRAFT_104661)
ABV: AGABI2DRAFT176670(AGABI2DRAFT_176670)
SLA: SERLADRAFT_357441(NADH-GOGAT)
MGL: MGL_2017
MRT: MRET_1549
PTI: PHATRDRAFT_51214(GltX)
 » show all
Reference
  Authors
Lancien M, Martin M, Hsieh MH, Leustek T, Goodman H, Coruzzi GM
  Title
Arabidopsis glt1-T mutant defines a role for NADH-GOGAT in the non-photorespiratory ammonium assimilatory pathway.
  Journal
Plant J 29:347-58 (2002)
DOI:10.1046/j.1365-313X.2002.01218.x
  Sequence
[ath:AT5G53460]
LinkDB

KEGG   ENZYME: 1.4.1.14
Entry
EC 1.4.1.14                 Enzyme                                 
Name
glutamate synthase (NADH);
glutamate (reduced nicotinamide adenine dinucleotide) synthase;
NADH: GOGAT;
L-glutamate synthase (NADH);
L-glutamate synthetase;
NADH-glutamate synthase;
NADH-dependent glutamate synthase
Class
Oxidoreductases;
Acting on the CH-NH2 group of donors;
With NAD+ or NADP+ as acceptor
Sysname
L-glutamate:NAD+ oxidoreductase (transaminating)
Reaction(IUBMB)
2 L-glutamate + NAD+ = L-glutamine + 2-oxoglutarate + NADH + H+ [RN:R00093];
(1a) L-glutamate + NH3 = L-glutamine + H2O [RN:R00256];
(1b) L-glutamate + NAD+ + H2O = NH3 + 2-oxoglutarate + NADH + H+ [RN:R00243]
Reaction(KEGG)
Substrate
L-glutamate [CPD:C00025];
NAD+ [CPD:C00003];
NH3 [CPD:C00014];
H2O [CPD:C00001]
Product
L-glutamine [CPD:C00064];
2-oxoglutarate [CPD:C00026];
NADH [CPD:C00004];
H+ [CPD:C00080];
H2O [CPD:C00001];
NH3 [CPD:C00014]
Comment
A flavoprotein (FMN). The reaction takes place in the direction of L-glutamate production. The protein is composed of two domains, one hydrolysing L-glutamine to NH3 and L-glutamate (cf. EC 3.5.1.2, glutaminase), the other combining the produced NH3 with 2-oxoglutarate to produce a second molecule of L-glutamate (cf. EC 1.4.1.2, glutamate dehydrogenase).
History
EC 1.4.1.14 created 1978, modified 2019
Pathway
ec00250  Alanine, aspartate and glutamate metabolism
ec00910  Nitrogen metabolism
ec01100  Metabolic pathways
ec01110  Biosynthesis of secondary metabolites
ec01120  Microbial metabolism in diverse environments
Orthology
K00264  glutamate synthase (NADH)
Genes
PTEH111534102 113223760 113223761
BFO118423229
BBEL109473320
SPU754163
DMEDmel_CG9674(CG9674)
DER6543630
DSE6606013
DSIDsimw501_GD12441(Dsim_GD12441)
DYADyak_GE22188
DAN6493372
DSR110190291
DPODpse_GA21956
DPE6601003
DMN108152363
DWI6645095
DGR6557958
DMODmoj_GI11930
DAZ108613420
DNV108650790
DHE111605684
DVI6622079
CCAT101448290
BOD106620726
MDE101893373
SCAC106092120
AGAAgaP_AGAP006360
ACOZ120951886
AARA120895665
AAG5565246
AALB109410517
CQUCpipJ_CPIJ007345
CPII120430753
CNS116345940
AME413372
ACER108004089
ALAB122714169
BIM100743936
BBIF117208172
BVK117235398
BVAN117154250
BTER100648506
BPYO122565625
CCAL108629149
OBB114879469
MGEN117218009
NMEA116432027
CGIG122398534
SOC105197574
MPHA105838501
AEC105149802
ACEP105627138
PBAR105424147
VEM105559493
HST105181045
DQU106749864
CFO105259420
FEX115245214
LHU105677426
PGC109859877
OBO105286430
PCF106785427
PFUC122514263
VPS122634919
NVI100122109
CSOL105368803
TPRE106656707
MDL103570096
CGLO123272834
FAS105270599
DAM107043748
AGIF122853354
CCIN107270167
TCA658584
DPA109543624
SOY115886449
ATD109601527
CSET123312571
AGB108917077
LDC111508893
APLN108732952
PPYR116171129
OTU111413251
MSEX115452078
BANY112047329
PPOT106100510
PXU106125288
PRAP110993503
ZCE119831402
OFU114358173
API100158883
DNX107164646
AGS114126871
RMD113555018
BTAB109029618
DCI103524615
CLEC106667559
HHAL106690746
NLU111052344
FOC113206005
ZNE110836732
CSEC111865708
DPXDAPPUDRAFT_110162
DMK116920522
PVM113799962
PJA122255183
PCHN125045218
HAME121879794
PCLA123755771
PTRU123520672
HAZT108678918
EAF111704323
DSV119461673
RSAN119395866
RMP119174150
VDE111247835
VJA111272506
DFR124499735
SDM118187282 118187991
CELCELE_W07E11.1(W07E11.1)
CBRCBG_01975
BGT106056462
GAE121371892
HRF124116337
HRJ124282667
CRG105337796
PMAX117327627
MMER123552518
OSN115223129
XEN124443157
ATHAT5G53460(GLT1)
ALY9300320
CRB17876423
CSAT104725863 104728554 104761363
EUSEUTSA_v10012414mg
BRP103857079
BNA106387482 106437523 111213076
BOE106332590
RSZ108845736
THJ104811925
CPAP110823143
CIT102620225
PVY116133280
MINC123198124 123206665 123226207
TCC18605743
GRA105765457
GHI107929529 107947250
GAB108479092
DZI111280486 111289287 111309227
EGR104437859
GMX100815931
GSJ114410308
PVUPHAVU_001G076400g
VRA106764633
VAR108331410
VUN114194130
CCAJ109814577
APRC113850812
MTRMTR_1g027020
CAM101509615
ADU107495014
AIP107605257
AHF112697424 112705922 112755343 112765389
LANG109326556
FVE101309397
RCN112183464
PPER18785174
PMUM103333633
PAVI110767133
PDUL117619118
MDM103443818
PXB103941488 103943433
ZJU107414598
MNT21390250
CSV101213224
CMO103485701
BHJ120081937
MCHA111011404
CMAX111482791
CMOS111430634
CPEP111810135
RCU8274353
JCU105648339
HBR110665757 110666168
MESC110617349 110630652
POP18103637 7453835
PEU105109026 105110510
PALZ118033501 118057296
JRE109004582 109013214
QSU112017959 112018592
QLO115959755
TWL119984320 120003889
VVI100246868
VRI117933639
SLY101254281
SPEN107013700
SOT102580516
CANN107855963
NSY104244568
NAU109213942
INI109183410
ITR116012569
SIND105160371
OEU111405628
EGT105971643
SSPL121747965 121752173 121779747 121782410
HAN110878172 110912724
ECAD122586618
LSV111918005
CCAV112508668 112515985
DCR108214500 108226948
CSIN114285508 114310758
BVG104888263
SOE110783090
CQI110696098 110728845
NNU104600364 104604004
MING122062882 122081042
TSS122640469 122666202
PSOM113299164 113358466
NCOL116266917
OSA4324398 4339561
DOSAOs05t0555600-01(Os05g0555600)
OBR102713634 102721892
BDI100832857 100844360
ATS109760356
TDC119268802 119279609
TAES123061864 123070558 123078967
SBI8068026
ZMA103636185 103651348
SITA101785947
SVS117857834
PVIR120664696 120675270 120697894 120707627
PHAI112894413
PDA103708325 103713334
EGU105039187 105041491
MUS103980032 103992734 103996064
DCT110110468
PEQ110020245
AOF109840747
ATR18442390
SMOSELMODRAFT_446658
PPP112283025
CRECHLRE_13g592200v5
VCNVOLCADRAFT_108038
APROF751_6615
MISMICPUN_105473(GLT)
MPPMICPUCDRAFT_57115(GLT)
SCEYDL171C(GLT1)
AGOAGOS_ADR290W
ERCEcym_4713
KLAKLLA0_E19625g
KMXKLMA_50304(GLT1)
LTHKLTH0B04708g
VPOKpol_530p21
ZROZYRO0B02596g
CGRCAGL0L01089g
NCSNCAS_0A14190(NCAS0A14190)
NDINDAI_0A01860(NDAI0A01860)
TPFTPHA_0F03070(TPHA0F03070)
TDLTDEL_0C01870(TDEL0C01870)
TGBHG536_0B05360
ZMKHG535_0B04610
DHADEHA2G15642g
PICPICST_87428(GLT1)
SPAASPAPADRAFT_141734(GLT1)
CALCAALFM_C106550WA(GLT1)
CTPCTRG_04234
COTCORT_0A06160
CDUCD36_06130
CTENCANTEDRAFT_101139
YLIYALI0B19998g
CLUCLUG_03565
CLUSA9F13_01g06028
CAURCJI97_000615
BNNFOA43_002177(GLT1)
BBRXBRETT_003814
NCRNCU01744(en(am)-2)
NTENEUTE1DRAFT65142(NEUTE1DRAFT_65142)
SMPSMAC_02431
PANPODANSg6535
TTTTHITE_2124366
MTMMYCTH_2312662
CTHRCTHT_0008270
MGRMGG_07187
TMNUCRPA7_4478
SSCKSPSK_08269
FGRFGSG_01433
FPUFPSE_08520
FVRFVEG_09511
FOXFOXG_11281
NHENECHADRAFT_57063
TRETRIREDRAFT_122287(glt1)
TRRM419DRAFT_24904
MAWMAC_00032
MAJMAA_01819
CMTCCM_03495
PLJVFPFJ_07201
CFJCFIO01_07667
SAPOSAPIO_CDS0981
ELAUCREL1_4299
PFYPFICI_11366
SSLSS1G_07849
BFUBCIN_15g02460(Bcglt1)
MBEMBM_04374
PSCOLY89DRAFT_691010
GLZGLAREA_03052
ANIAN5134.2
AFMAFUA_1G07380
ACTACLA_027210
NFINFIA_017350
AORAO090012000988
ANGANI_1_1272064(An07g09920)
AFVAFLA_002716
ALUCAKAW2_11294S(GLT1)
APUUAPUU_41225A(GLT1)
PCSPc22g11310
PDPPDIP_26070
TMFPMAA_097140
TRGTRUGW13939_11475
CIMCIMG_02486
CPWCPC735_041070
UREUREG_03225
PBLPAAG_12483(PAAG_07998)
PBNPADG_02101
ABEARB_04215
TVETRV_01094
BGHBDBG_06065
PTEPTT_08692
BZECOCCADRAFT_4567
BSCCOCSADRAFT_185254
BORCOCMIDRAFT_10233
AALTCC77DRAFT_261749
ZTRMYCGRDRAFT_110982
PFJMYCFIDRAFT_52899
BCOMBAUCODRAFT_475239
NPAUCRNP2_1000
TMLGSTUM_00002450001
SPOSPAPB1E7.07(glt1)
CNECNJ02910
CNBCNBJ0620
CGICGB_I4060C
TMSTREMEDRAFT_42578
TASAA1Q1_06977
TVSTRAVEDRAFT_27775
DSQDICSQDRAFT_177406
PCOPHACADRAFT_127541
SHSSTEHIDRAFT_74778
HIRHETIRDRAFT_433732
PSQPUNSTDRAFT_144302
ADLAURDEDRAFT_114408
FMEFOMMEDRAFT_166121
GTRGLOTRDRAFT_137109
LBCLACBIDRAFT_183838
MRRMoror_14828
CCICC1G_13933
SCMSCHCODRAFT_74235
ABPAGABI1DRAFT104661(AGABI1DRAFT_104661)
ABVAGABI2DRAFT176670(AGABI2DRAFT_176670)
CPUTCONPUDRAFT_80026
SLASERLADRAFT_357441(NADH-GOGAT)
UMAUMAG_03850
PFPPFL1_06431
MGLMGL_2017
MRTMRET_1549
MSYMMSY001_1099
MBRMONBRDRAFT_15256
PFAPF3D7_1435300
PFDPFDG_02810
PFHPFHG_00264
PREIPRSY57_1434600
PBEPBANKA_1009500
PVVPVVCY_0801000
PKNPKNH_0421900
PVXPVX_084770
PCYPCYB_132360
PTIPHATRDRAFT_51214(GltX)
TPSTHAPSDRAFT_29861
 » show all
Reference
1  [PMID:4362465]
  Authors
Roon RJ, Even HL, Larimore F
  Title
Glutamate synthase: properties of the reduced nicotinamide adenine dinucleotide-dependent enzyme from Saccharomyces cerevisiae.
  Journal
J Bacteriol 118:89-95 (1974)
DOI:10.1128/JB.118.1.89-95.1974
Reference
2  [PMID:21790]
  Authors
Boland MJ, Benny AG.
  Title
Enzymes of nitrogen metabolism in legume nodules. Purification and properties of NADH-dependent glutamate synthase from lupin nodules.
  Journal
Eur J Biochem 79:355-62 (1977)
DOI:10.1111/j.1432-1033.1977.tb11816.x
Reference
3  [PMID:7047525]
  Authors
Masters DS Jr, Meister A
  Title
Inhibition of homocysteine sulfonamide of glutamate synthase purified from Saccharomyces cerevisiae.
  Journal
J Biol Chem 257:8711-5 (1982)
Reference
4  [PMID:16666762]
  Authors
Anderson MP, Vance CP, Heichel GH, Miller SS
  Title
Purification and Characterization of NADH-Glutamate Synthase from Alfalfa Root Nodules.
  Journal
Plant Physiol 90:351-8 (1989)
DOI:10.1104/pp.90.1.351
Reference
5  [PMID:18182018]
  Authors
Blanco L, Reddy PM, Silvente S, Bucciarelli B, Khandual S, Alvarado-Affantranger X, Sanchez F, Miller S, Vance C, Lara-Flores M
  Title
Molecular cloning, characterization and regulation of two different NADH-glutamate synthase cDNAs in bean nodules.
  Journal
Plant Cell Environ 31:454-72 (2008)
DOI:10.1111/j.1365-3040.2008.01774.x
Other DBs
ExplorEnz - The Enzyme Database: 1.4.1.14
IUBMB Enzyme Nomenclature: 1.4.1.14
ExPASy - ENZYME nomenclature database: 1.4.1.14
BRENDA, the Enzyme Database: 1.4.1.14
CAS: 65589-88-0
LinkDB

KEGG   REACTION: R00093
Entry
R00093                      Reaction                               
Name
L-glutamate:NAD+ oxidoreductase (transaminating)
Definition
2 L-Glutamate + NAD+ <=> L-Glutamine + 2-Oxoglutarate + NADH + H+
Equation
Reaction class
RC00001  C00003_C00004
RC00006  C00025_C00026
RC00010  C00025_C00064
Enzyme
Pathway
rn00250  Alanine, aspartate and glutamate metabolism
rn00910  Nitrogen metabolism
rn01100  Metabolic pathways
rn01110  Biosynthesis of secondary metabolites
rn01120  Microbial metabolism in diverse environments
Orthology
K00264  glutamate synthase (NADH) [EC:1.4.1.14]
Other DBs
RHEA: 13756
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