KEGG   ORTHOLOGY: K03146
Entry
K03146                      KO                                     
Symbol
THI4, THI1
Name
cysteine-dependent adenosine diphosphate thiazole synthase [EC:2.4.2.60]
Pathway
map00730  Thiamine metabolism
map01100  Metabolic pathways
map01240  Biosynthesis of cofactors
Module
M00897  Thiamine biosynthesis, plants, AIR (+ NAD+) => TMP/thiamine/TPP
M00898  Thiamine biosynthesis, pyridoxal-5P => TMP/thiamine/TPP
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09108 Metabolism of cofactors and vitamins
   00730 Thiamine metabolism
    K03146  THI4, THI1; cysteine-dependent adenosine diphosphate thiazole synthase
Enzymes [BR:ko01000]
 2. Transferases
  2.4  Glycosyltransferases
   2.4.2  Pentosyltransferases
    2.4.2.60  cysteine-dependent adenosine diphosphate thiazole synthase
     K03146  THI4, THI1; cysteine-dependent adenosine diphosphate thiazole synthase
Other DBs
RN: R10685
COG: COG1635
Genes
ATH: AT5G54770(THI1)
ALY: 9300430
CRB: 17876896
CSAT: 104726000 104731059 104763460
EUS: EUTSA_v10013978mg
BRP: 103844930 103851941 103857026
BNA: 106346007 106347311 106349644 106366413 106371241 106420919 106420920 106439989
BOE: 106318832 106327201 106335239
RSZ: 108820570 108836615 108837050 108844151
THJ: 104817652
CPAP: 110810965
PVY: 116123427
EGR: 104450213
APRC: 113872364
LJA: Lj3g3v1010900.1(Lj3g3v1010900.1) Lj5g3v2060670.1(Lj5g3v2060670.1)
PPER: 18780719
PMUM: 103342510
PDUL: 117623858
ZJU: 107420469
MNT: 21395127
CSV: 101211553
CMO: 103501605
BHJ: 120091480
RCU: 8280736
JCU: 105632894
HBR: 110647144
VVI: 100260728(THI1-1) 100265774(THI1-2)
SLY: 101257192
SPEN: 107025837
SOT: 102598996
CANN: 107856341
NTA: 107797059(ThiI) 107798781
NSY: 104224143
NTO: 104087540
NAU: 109232136
SOE: 110795721
NNU: 104604883
TSS: 122640918
NCOL: 116249223
OSA: 4343443
DOSA: Os07t0529600-01(Os07g0529600)
OBR: 102709384
BDI: 100841709
ATS: 109771029
PHAI: 112889419
DCT: 110108175
PEQ: 110031149
ATR: 18435056
APRO: F751_1708
SCE: YGR144W(THI4)
ERC: Ecym_6012
KMX: KLMA_40625(THI4)
NCS: NCAS_0D01750(NCAS0D01750)
NDI: NDAI_0C02860(NDAI0C02860)
TPF: TPHA_0A04790(TPHA0A04790)
TBL: TBLA_0B09390(TBLA0B09390) TBLA_0H00760(TBLA0H00760)
TDL: TDEL_0H00200(TDEL0H00200)
CAL: CAALFM_C305130CA(THI4)
SLB: AWJ20_4223(THI4)
BNN: FOA43_001955(STI35)
BBRX: BRETT_001261(THI4)
NCR: NCU06110
NTE: NEUTE1DRAFT95662(NEUTE1DRAFT_95662)
MGR: MGG_03098
SSCK: SPSK_07738
TRE: TRIREDRAFT_68608(thi4)
MAW: MAC_01186
MAJ: MAA_05549
CMT: CCM_01639
BFU: BCIN_07g06010(Bcthi4)
MBE: MBM_01288
ANI: AN3928.2
ANG: ANI_1_228094(An11g01630)
ALUC: AKAW2_80142S(THI4)
ACHE: ACHE_80625S(THI4)
APUU: APUU_40431S(THI4)
ABE: ARB_07940
TVE: TRV_04098
PTE: PTT_08482
SPO: SPBC26H8.01(thi2)
ABP: AGABI1DRAFT40074(AGABI1DRAFT_40074)
ABV: AGABI2DRAFT72787(AGABI2DRAFT_72787)
WSE: WALSEDRAFT_32181(CYPBP37)
MGL: MGL_1314
SMIN: v1.2.003820.t1(symbB.v1.2.003820.t1)
SPAR: SPRG_04526
 » show all
Reference
  Authors
Chatterjee A, Abeydeera ND, Bale S, Pai PJ, Dorrestein PC, Russell DH, Ealick SE, Begley TP
  Title
Saccharomyces cerevisiae THI4p is a suicide thiamine thiazole synthase.
  Journal
Nature 478:542-6 (2011)
DOI:10.1038/nature10503
  Sequence
[sce:YGR144W]
LinkDB

KEGG   ORTHOLOGY: K22699
Entry
K22699                      KO                                     
Symbol
thi4
Name
sulfide-dependent adenosine diphosphate thiazole synthase [EC:2.4.2.59]
Pathway
map00730  Thiamine metabolism
map01100  Metabolic pathways
map01240  Biosynthesis of cofactors
Module
M00896  Thiamine biosynthesis, archaea, AIR (+ NAD+) => TMP/TPP
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09108 Metabolism of cofactors and vitamins
   00730 Thiamine metabolism
    K22699  thi4; sulfide-dependent adenosine diphosphate thiazole synthase
Enzymes [BR:ko01000]
 2. Transferases
  2.4  Glycosyltransferases
   2.4.2  Pentosyltransferases
    2.4.2.59  sulfide-dependent adenosine diphosphate thiazole synthase
     K22699  thi4; sulfide-dependent adenosine diphosphate thiazole synthase
Other DBs
RN: R12026
COG: COG1635
Genes
DSD: GD606_19515
DMA: DMR_31420
DCB: C3Y92_06440
DGG: DGI_1133
DMS: E8L03_06730
DAF: Desaf_0994
DSX: GD604_13095
DOL: Dole_2426
DAL: Dalk_2394
DOV: DSCO28_45120
DLI: dnl_00920(thiG)
DMM: dnm_073520(thiG)
DBR: Deba_1981
MALU: KU6B_16750
CSQ: CSCA_1812
DAU: Daud_0859
TACI: TDSAC_0747
MBX: BCGT_0584
MPHL: MPHLCCUG_05062(ksdD_3)
TAI: Taci_1358
TLI: Tlie_1851
PDI: BDI_1609
MBAS: ALGA_3764
PAA: Paes_1007
TTK: TST_0727(thi1)
TMA: TM0787
TMW: THMA_0806
TMQ: THMB_0806
TMX: THMC_0806
TPT: Tpet_0142
TRQ: TRQ2_0140
TNA: CTN_1791
TNP: Tnap_0143
TLE: Tlet_1750
TME: Tmel_1590
TAF: THA_1798
THER: Y592_08765
FNO: Fnod_1422
NDE: NIDE1062
NJA: NSJP_2079
CTHI: THC_0653
MJA: MJ_0601
MESG: MLAUSG7_1377(thi)
MESA: MLASG1_0803(thi)
MMP: MMP1352
MMAD: MMJJ_14870
MAE: Maeo_0798
MVO: Mvol_0738
MTH: MTH_1620
METC: MTCT_1479
MWO: MWSIV6_0166(Thi4)
METE: tca_01569
MEB: Abm4_1461
MFV: Mfer_0175
MKA: MK0430
AFU: AF_0702
FPL: Ferp_0628
GAC: GACE_0145
GAH: GAH_01007
PFU: PF1530
PFI: PFC_06880
PHO: PH1357(PH1357)
PAB: PAB0536(thi1)
PYS: Py04_1231
TKO: TK0434
TGA: TGAM_1270
THE: GQS_02015
THM: CL1_0036
TLT: OCC_06556
THS: TES1_1982
TNU: BD01_0513
TEU: TEU_09505
TCQ: TIRI35C_0994(thi)
PPAC: PAP_08770
MBAR: MSBR2_2375
MBAK: MSBR3_2431
MAC: MA_2851
MMA: MM_0222
MMAC: MSMAC_2441
METM: MSMTP_1119
MTHR: MSTHT_0054
MTHE: MSTHC_0676
MHOR: MSHOH_1299
MBU: Mbur_2109
MMH: Mmah_0298
MTP: Mthe_1121
MCJ: MCON_3229
MHI: Mhar_2378
MHU: Mhun_1935
MLA: Mlab_0277
MBG: BN140_1460(thi1)
MEMA: MMAB1_1869
MPI: Mpet_0116
MEZ: Mtc_1604(thi4-1) Mtc_2398(thi4-2)
HAL: VNG_2604G(thi1)
HSL: OE_4651F(thi4)
HHB: Hhub_1122(thi4)
HARA: AArcS_1966(thi4)
HMA: rrnAC1782(thi1)
HHI: HAH_2313(thi1)
NPH: NP_5174A(thi4)
NMO: Nmlp_3882(thi4)
HUT: Huta_2718
HTI: HTIA_2465
HMU: Hmuk_0002
HALL: LC1Hm_2563(thi4)
HDS: HSR122_2321(thi4)
HWA: HQ_1276A(thi4)
HWC: Hqrw_1306(thi4)
HVO: HVO_0665(thi4)
HME: HFX_0634
HLA: Hlac_2980
HDF: AArcSl_1355(thi4)
HTU: Htur_1462
NMG: Nmag_2419(thi4)
NAT: NJ7G_3680
SALI: L593_09560
MELU: MTLP_07010
ABI: Aboo_0963
ACJ: ACAM_1344
IHO: Igni_0560
IIS: EYM_05510
DKA: DKAM_0713
IAG: Igag_0295
HBU: Hbut_0253
STO: STK_03610
SSO: SSO0436
SOL: Ssol_1411
SSOA: SULA_1456
SSOL: SULB_1457
SSOF: SULC_1455
SCAS: SACC_05940
SAI: Saci_0854(thi)
SID: M164_1717
SII: LD85_1927
SIH: SiH_1645
SIR: SiRe_1566
SIC: SiL_1558
MSE: Msed_2221
MCN: Mcup_0086
AHO: Ahos_0692
ACIH: HS5_20660
CSTY: KN1_20130
STEP: IC006_1529
SAHS: HS7_05390
PAI: PAE0175
PIS: Pisl_1998
PCL: Pcal_1555
PAS: Pars_1498
PYR: P186_0936
POG: Pogu_0708
TNE: Tneu_1052
CMA: Cmaq_1420
TUZ: TUZN_0079
VDI: Vdis_2373
VMO: VMUT_0723
NMR: Nmar_0678
NCT: NMSP_1025
NEV: NTE_02257
NFN: NFRAN_0414(thi)
NCV: NCAV_0396(thi)
NBV: T478_0555
NDV: NDEV_0758
CCAI: NAS2_0821
KCR: Kcr_0795
BARB: AOA66_0043
 » show all
Reference
  Authors
Eser BE, Zhang X, Chanani PK, Begley TP, Ealick SE
  Title
From Suicide Enzyme to Catalyst: The Iron-Dependent Sulfide Transfer in Methanococcus jannaschii Thiamin Thiazole Biosynthesis.
  Journal
J Am Chem Soc 138:3639-42 (2016)
DOI:10.1021/jacs.6b00445
  Sequence
[mja:MJ_0601]
Reference
  Authors
Hwang S, Cordova B, Chavarria N, Elbanna D, McHugh S, Rojas J, Pfeiffer F, Maupin-Furlow JA
  Title
Conserved active site cysteine residue of archaeal THI4 homolog is essential for thiamine biosynthesis in Haloferax volcanii.
  Journal
BMC Microbiol 14:260 (2014)
DOI:10.1186/s12866-014-0260-0
  Sequence
[hvo:HVO_0665]
LinkDB

KEGG   ENZYME: 2.4.2.59
Entry
EC 2.4.2.59                 Enzyme                                 
Name
sulfide-dependent adenosine diphosphate thiazole synthase;
Thi4 (ambiguous)
Class
Transferases;
Glycosyltransferases;
Pentosyltransferases
Sysname
NAD+:glycine ADP-D-ribosyltransferase (sulfide-adding)
Reaction(IUBMB)
NAD+ + glycine + sulfide = nicotinamide + ADP-5-ethyl-4-methylthiazole-2-carboxylate + 3 H2O [RN:R12026]
Reaction(KEGG)
R12026
Substrate
NAD+ [CPD:C00003];
glycine [CPD:C00037];
sulfide [CPD:C00283]
Product
nicotinamide [CPD:C00153];
ADP-5-ethyl-4-methylthiazole-2-carboxylate [CPD:C20784];
H2O [CPD:C00001]
Comment
This iron dependent enzyme, found in most archaea, is involved in the biosynthesis of thiamine phosphate. The homologous enzyme from plants and fungi (EC 2.4.2.60, cysteine-dependent adenosine diphosphate thiazole synthase) uses an intrinsic cysteine as sulfur donor and, unlike the archaeal enzyme, is a single turn-over enzyme.
History
EC 2.4.2.59 created 2018
Pathway
ec00730  Thiamine metabolism
ec01100  Metabolic pathways
Orthology
K22699  sulfide-dependent adenosine diphosphate thiazole synthase
Genes
DSDGD606_19515
DMADMR_31420
DCBC3Y92_06440
DGGDGI_1133
DMSE8L03_06730
DAFDesaf_0994
DSXGD604_13095
DOLDole_2426
DALDalk_2394
DOVDSCO28_45120
DLIdnl_00920(thiG)
DMMdnm_073520(thiG)
DAODesac_0998
DAXFDQ92_03460
DBRDeba_1981
HMRHipma_0421
DAVDESACE_08810
DHRLGS26_09060
BBARRHAL1_03078
CAULKCG34_24290
MALUKU6B_16750
LDLLBU_0926 LBU_0941
CLJCLJU_c30580
CSQCSCA_1812
CDRKB9W14_10240
CFERD4Z93_11890
CLOSDMR38_15480
DKUDesku_1659
DAUDaud_0859
TJRTherJR_1442
ADGAdeg_0421 Adeg_1609
GEKkuro4_04070
TNRThena_0778
TACITDSAC_0747
MBXBCGT_0584
MPHLMPHLCCUG_05062(ksdD_3)
SLKSLUN_34370
HBSIPV69_04150
VAIBU251_06310 BU251_06360
TAITaci_1358
TLITlie_1851
AMOAnamo_0225
BCELBcellWH2_01838
BISDXK01_001245
PDIBDI_1609
PARCCI960_06610
PMERINE87_00229
PGOLK6V26_06880
PJOLK449_05640
PMZHMPREF0659_A5800
PITPIN17_A0608
PNIJ5A59_03980
PHERprwr041_11060
RBCBN938_1539
TTZFHG85_07515
MBASALGA_3764
PAAPaes_1007
PROCPtc2401_01068
PRSB9H02_06355
PROSCHL67_06345
TTKTST_0727(thi1)
TAMTheam_0077
DTEDester_1473
TMATM0787
TMMTmari_0788
TMITHEMA_00715
TMWTHMA_0806
TMQTHMB_0806
TMXTHMC_0806
TPTTpet_0142
TRQTRQ2_0140
TNACTN_1791
TNPTnap_0143
THQT2812B_00715
THZCELL2_00740
THRTRQ7_00715
TLETlet_1750
TTATheth_1374
TMETmel_1590
TAFTHA_1798
THPBG95_08660
THERY592_08765
FNOFnod_1422
FPEFerpe_0640
FIANA23_03490
NDENIDE1062
NMVNITMOv2_0947
NIONITINOP_1921
NJANSJP_2079
NKFNkreftii_003088
TIDThein_1804
TOPTOPB45_0915
TCMHL41_01820
THETF1847_03705
CTHITHC_0653
TAVG4V39_01630
TMAIFVE67_08730
MJAMJ_0601
MFEMefer_1385
MVUMetvu_1585
MFSMFS40622_1580
MIFMetin_1281
MJHJH146_0825
MESGMLAUSG7_1377(thi)
MESAMLASG1_0803(thi)
MIGMetig_0735 Metig_1112
MMPMMP1352
MMQMmarC5_0226
MMXMmarC6_1321
MMZMmarC7_0597
MMAKMMKA1_15750
MMAOMMOS7_14560
MMADMMJJ_14870
MAEMaeo_0798
MVNMevan_0663
MVOMvol_0738
MOKMetok_0500
METFCFE53_00030
MTHMTH_1620
MMGMTBMA_c02060
METCMTCT_1479
MWOMWSIV6_0166(Thi4)
METEtca_01569
METZMETMT2_1460
METKFVF72_03520
MTHMFZP57_00720
METJFZP68_05735
MEBAbm4_1461
MELMetbo_0536 Metbo_1265
MEWMSWAN_0974 MSWAN_1853
METHMBMB1_1137 MBMB1_1486
MFCBRM9_0713 BRM9_1347
MFIDSM1535_0665 DSM1535_2031
MCUBMCBB_1020 MCBB_1725
MSUBBK009_06380
METNBK008_01970
METTCIT01_06780
METOCIT02_02510 CIT02_10600
MEMEHYG87_01485 HYG87_07505
MFVMfer_0175
MKAMK0430
AFUAF_0702
AFGAFULGI_00007840
APOArcpr_1423
AVEArcve_1405
ASTAsulf_00980
FPLFerp_0628
GACGACE_0145
GAHGAH_01007
PFUPF1530
PFIPFC_06880
PHOPH1357(PH1357)
PABPAB0536(thi1)
PYNPNA2_1583 PNA2_1941
PYAPYCH_06240 PYCH_08650
PYSPy04_1231
PYCTQ32_10505
TKOTK0434
TGATGAM_1270
THEGQS_02015
THMCL1_0036
TLTOCC_06556
THSTES1_1982
TNUBD01_0513
TEUTEU_09505
TGYX802_10050
THVADU37_CDS17350 ADU37_CDS22280
TCHCHITON_1554
TPEPA0127_02260
TPIEA7C91_09070
TCEA3L02_01985
TTDA3L14_05500
TPRFA3L09_06395
TPAFA3L08_06895
THYA3L12_03095
TICFH039_06460
TCQTIRI35C_0994(thi)
THEMFPV09_02920
PPACPAP_08770
MBAMbar_A2100
MBYMSBRM_2409
MBWMSBRW_2643
MBARMSBR2_2375
MBAKMSBR3_2431
MACMA_2851
MMAMM_0222
MMAZMmTuc01_0227
MMJMSMAS_1078
MMACMSMAC_2441
MVCMSVAZ_1117
MEKMSKOL_1072
MLSMSLAZ_1169
METMMSMTP_1119
MEFMSWH1_1762
MEQMSWHS_1554
MSJMSSAC_2840
MSZMSSIH_2497
MSWMSSIT_2540
MTHRMSTHT_0054
MTHEMSTHC_0676
MHORMSHOH_1299
MFZAOB57_002245
MBUMbur_2109
MMETMCMEM_0306 MCMEM_0840
MELOJ7W08_02430 J7W08_11625
MMHMmah_0298
MHAZBHR79_02030
MEVMetev_0188
MZHMzhil_1372
MZIHWN40_08730
MTPMthe_1121
MCJMCON_3229
MHIMhar_2378
MHUMhun_1935
MRTJKHC33_14740
MLAMlab_0277
MEMMemar_2251
MBGBN140_1460(thi1)
MEMAMMAB1_1869
MCHKMchiMG62_25690
MPIMpet_0116
MFOMetfor_0238
MEZMtc_1604(thi4-1) Mtc_2398(thi4-2)
HALVNG_2604G(thi1)
HSLOE_4651F(thi4)
HHBHhub_1122(thi4)
HAGSJT689_11965
HABOJRZ79_10305
HNOLT974_00705
HLULT972_12875
HANRLJ422_10530
HJEHacjB3_12970
HSUHLASF_0331
HSFHLASA_0330
SALRFQU85_03530
HALREFA46_011875
HARAAArcS_1966(thi4)
HAADMW046_08650
HALXM0R89_10525
HMArrnAC1782(thi1)
HHIHAH_2313(thi1)
HHNHISP_11780
HABSG26_04110
HTABVU17_11255
HALJG9465_07010
HSINKDQ40_01865
NPHNP_5174A(thi4)
NMONmlp_3882(thi4)
NHOHWV23_12010
NSALHWV07_14560
HUTHuta_2718
HTIHTIA_2465
HALAHrd1104_02040
HMUHmuk_0002
HALZE5139_03335
HALLLC1Hm_2563(thi4)
HAZPGBQ70_03335
HALIBV210_05585
HSNDV733_09670
HDSHSR122_2321(thi4)
HRRHZS55_08655
HPELHZS54_09780
HLTI7X12_05325
HARCHARCEL1_09615
HDOMUK72_07875
HWAHQ_1276A(thi4)
HWCHqrw_1306(thi4)
HVOHVO_0665(thi4)
HMEHFX_0634
HGIABY42_03175
HALEG3A49_03390
HBOHbor_25460
HAQDU484_06390
HAJDU500_06660
HAERDU502_04335
HRAEI982_09275
HLMDV707_12630
HLAHlac_2980
HALBEKH57_08560
HEZZEO776_03960
HALQHrr1229_002890
HSSJ7656_12905
HSAIHPS36_03250
HAZZKI388_01415
SRUBC2R22_02145
HAEhalTADL_1093
HALNB4589_006725
HALGHUG10_03970
HALUHUG12_12960
HMPK6T50_02080
HREK6T36_01900
HRMK6T25_14540
HACBHbl1158_08970
HDFAArcSl_1355(thi4)
HAHHalar_3704
HTUHtur_1462
HDABB347_01930
HJTDVR14_04265
HALYHYG82_19015
HSALJMJ58_01385
HLOJ0X27_03870
HAKZJ0X25_14155
NMGNmag_2419(thi4)
HXAHalxa_2863
NATNJ7G_3680
NPENatpe_2830
NVRFEJ81_13195
NPLFGF80_13125
NAYHYG81_16885
NGENatgr_0926
HRUHalru_1150
NOUNatoc_0532
SALIL593_09560
SAIMK0C01_03160
HLRHALLA_10200
HLCCHINAEXTREME16945
NAJB1756_03455
NAGAArcMg_1121
NANAArc1_2501
NBGDV706_12525
NASGCU68_13250
MELUMTLP_07010
MEAMMU439_01690
ABIAboo_0963
ACFAciM339_1016
APEAPE_2149.1
ACJACAM_1344
SMRSmar_0052 Smar_0180
SHCShell_0764
IHOIgni_0560
IISEYM_05510
DKADKAM_0713
DFDDesfe_0795
IAGIgag_0295
THGTCELL_1023
HBUHbut_0253
PFMPyrfu_0366
PDLPyrde_0220 Pyrde_0347
STOSTK_03610
SOHD1869_01525
SSOSSO0436
SOLSsol_1411
SSOASULA_1456
SSOLSULB_1457
SSOFSULC_1455
SSHIJ5U23_03002
SCASSACC_05940
SAISaci_0854(thi)
SACNSacN8_04145
SACRSacRon12I_04135
SACSSUSAZ_03885
SISLS215_1798
SIAM1425_1670
SIMM1627_1786
SIDM164_1717
SIYYG5714_1786
SINYN1551_1140
SIILD85_1927
SIHSiH_1645
SIRSiRe_1566
SICSiL_1558
SULABFU36_03640
SULEGFS03_09395
SULOGFS33_01210
SULLEWF20_02995
MSEMsed_2221
MCNMcup_0086
MHKDFR87_07430
MPRUDFR88_07115
MTENGWK48_04735
MJNMjAS7_0513
AHOAhos_0692
AMANB6F84_06895
ABRIDFR85_08045
ASULDFR86_05955
AAMBD1866_11245
ACIHHS5_20660
SACDHS1genome_2409
SAZOD1868_02625
CSTYKN1_20130
STEPIC006_1529
SAHSHS7_05390
PAIPAE0175
PISPisl_1998
PCLPcal_1555
PASPars_1498
PYRP186_0936
POGPogu_0708
TNETneu_1052
PYWPYWP30_02101
CMACmaq_1420
TUZTUZN_0079
VDIVdis_2373
VMOVMUT_0723
FFOFFONT_0392
NMRNmar_0678
NIRNSED_03435
NKRNKOR_03495
NIDNPIRD3C_1584
NINNADRNF5_1474
NIWNisw_01510
NCLC5F47_05695
NOXC5F49_05585
NUEC5F50_04895
NIPNsoK4_05025
NCTNMSP_1025
NICDSQ20_03585
CSYCENSYa_0361
NGANgar_c21660
NVNNVIE_008130
NEVNTE_02257
TAANMY3_02935
NFNNFRAN_0414(thi)
NCVNCAV_0396(thi)
NBVT478_0555
TAHSU86_002805
NIUDSQ19_03795
NDVNDEV_0758
CCAINAS2_0821
KCRKcr_0795
BARBAOA66_0043
FLTSv326_1000
 » show all
Reference
1  [PMID:26919468]
  Authors
Zhang X, Eser BE, Chanani PK, Begley TP, Ealick SE
  Title
Structural Basis for Iron-Mediated Sulfur Transfer in Archael and Yeast Thiazole  Synthases.
  Journal
Biochemistry 55:1826-38 (2016)
DOI:10.1021/acs.biochem.6b00030
  Sequence
[mja:MJ_0601] [mig:Metig_0735]
Reference
2  [PMID:26928142]
  Authors
Eser BE, Zhang X, Chanani PK, Begley TP, Ealick SE
  Title
From Suicide Enzyme to Catalyst: The Iron-Dependent Sulfide Transfer in Methanococcus jannaschii Thiamin Thiazole Biosynthesis.
  Journal
J Am Chem Soc 138:3639-42 (2016)
DOI:10.1021/jacs.6b00445
  Sequence
[mja:MJ_0601]
Other DBs
ExplorEnz - The Enzyme Database: 2.4.2.59
IUBMB Enzyme Nomenclature: 2.4.2.59
ExPASy - ENZYME nomenclature database: 2.4.2.59
BRENDA, the Enzyme Database: 2.4.2.59
LinkDB

KEGG   ENZYME: 2.4.2.60
Entry
EC 2.4.2.60                 Enzyme                                 
Name
cysteine-dependent adenosine diphosphate thiazole synthase;
THI4 (gene name) (ambiguous);
THI1 (gene name);
ADP-thiazole synthase
Class
Transferases;
Glycosyltransferases;
Pentosyltransferases
Sysname
NAD+:glycine ADP-D-ribosyltransferase (dehydroalanine-producing)
Reaction(IUBMB)
NAD+ + glycine + [ADP-thiazole synthase]-L-cysteine = nicotinamide + ADP-5-ethyl-4-methylthiazole-2-carboxylate + [ADP-thiazole synthase]-dehydroalanine + 3 H2O [RN:R10685]
Reaction(KEGG)
R10685
Substrate
NAD+ [CPD:C00003];
glycine [CPD:C00037];
[ADP-thiazole synthase]-L-cysteine
Product
nicotinamide [CPD:C00153];
ADP-5-ethyl-4-methylthiazole-2-carboxylate [CPD:C20784];
[ADP-thiazole synthase]-dehydroalanine;
H2O [CPD:C00001]
Comment
This iron dependent enzyme, found in fungi, plants, and some archaea, is involved in the thiamine phosphate biosynthesis pathway. It is a single turn-over enzyme since the cysteine residue is not regenerated in vivo [3]. The homologous enzyme in archaea (EC 2.4.2.59, sulfide-dependent adenosine diphosphate thiazole synthase) uses sulfide as sulfur donor.
History
EC 2.4.2.60 created 2018
Pathway
ec00730  Thiamine metabolism
ec01100  Metabolic pathways
Orthology
K03146  cysteine-dependent adenosine diphosphate thiazole synthase
Genes
ATHAT5G54770(THI1)
ALY9300430
CRB17876896
CSAT104726000 104731059 104763460
EUSEUTSA_v10013978mg
BRP103844930 103851941 103857026
BNA106346007 106347311 106349644 106366413 106371241 106420919 106420920 106439989
BOE106318832 106327201 106335239
RSZ108820570 108836615 108837050 108844151
THJ104817652
CPAP110810965
CIT102626594 102629135
CICCICLE_v10008765mg CICLE_v10015727mg CICLE_v10024231mg
PVY116123427
MINC123202328 123225908
TCC18593644 18597097
GRA105769824 105773933 105780320
GHI107901923 107917921 107928645 107958248 107961959 121217764
GAB108453783 108482152 108486471
DZI111280819 111301627 111318798
EGR104450213
GMX100775614 100788953 100794288 547461(SC-04) 547462(SC-03)
GSJ114372051 114380540 114403613 114404912 114417056
PVUPHAVU_007G052900g PHAVU_011G139100g
VRA106754377 106755182
VAR108325373 108339541
VUN114168645 114192234
CCAJ109795024 109808173
APRC113872364
MTRMTR_1g105480 MTR_4g081130
CAM101491901 101507074 101511321 101512227
LJALj3g3v1010900.1(Lj3g3v1010900.1) Lj5g3v2060670.1(Lj5g3v2060670.1)
ADU107477997 107488645
AIP107630145 107643278
AHF112737808 112749911 112789941 112801227
LANG109331169 109332649 109348532 109351883
FVE101298688 101299651
RCN112168087 112201505 112201899
PPER18780719
PMUM103342510
PDUL117623858
MDM103429668 103448581
PXB103948770 103967326
ZJU107420469
MNT21395127
CSV101211553
CMO103501605
BHJ120091480
CMAX111483293 111487533
CMOS111438464 111448035
CPEP111783834 111785935 111788519
RCU8280736
JCU105632894
HBR110647144
MESC110601845 110611614
POP7470933 7479083 7485534
PEU105108187 105130169 105134848
PALZ118035853 118047445 118059587
JRE108983682 108993518 109022013
QSU111989411 111993711 112014551 112016278
QLO115956034 115956545 115972735 115988494
TWL119988758 119988861
VVI100260728(THI1-1) 100265774(THI1-2)
VRI117909200 117923467
SLY101257192
SPEN107025837
SOT102598996
CANN107856341
NTA107797059(ThiI) 107798781
NSY104224143
NTO104087540
NAU109232136
INI109167290 109187908
ITR116005701 116028414
SIND105167970 105175375
OEU111383503 111389379
EGT105948772 105962248
SSPL121740783 121742722 121743409 121757713 121775363 121800880
HAN110890327 110912864
ECAD122591292 122611091
LSV111877285 111914386
CCAV112507403 112514948
DCR108199579 108204812 108211831
CSIN114275070 114297269
BVG104887771 104887775
SOE110795721
CQI110721030 110723750
NNU104604883
MING122078253 122091022
TSS122640918
PSOM113279575 113284440 113287867 113288375
NCOL116249223
OSA4343443
DOSAOs07t0529600-01(Os07g0529600)
OBR102709384
BDI100841709
ATS109771029
TDC119281869 119330895 119338169
TAES123066078 123148177 123160697 123169109
SBI8063504 8078187
ZMA542714 542715
SITA101752840 101774400
SVS117842602 117853273
PVIR120671321 120704075
PHAI112889419
PDA103716822 103721429 120107672
EGU105046898 105051965
MUS103975582 103991030
DCT110108175
PEQ110031149
AOF109827745 109834756
ATR18435056
SMOSELMODRAFT_163429 SELMODRAFT_271002
PPP112273035 112273481 112275550 112276032 112276579
CRECHLRE_04g214150v5
VCNVOLCADRAFT_74899 VOLCADRAFT_74900
MNGMNEG_15292 MNEG_7985
CSLCOCSUDRAFT_14398
CVRCHLNCDRAFT_22703
APROF751_1708
SCEYGR144W(THI4)
AGOAGOS_AAL137W
ERCEcym_6012
KLAKLLA0_A00198g
KMXKLMA_40625(THI4)
LTHKLTH0B03916g
VPOKpol_1050p31
ZROZYRO0G11726g
CGRCAGL0M01166g
NCSNCAS_0D01750(NCAS0D01750)
NDINDAI_0C02860(NDAI0C02860)
TPFTPHA_0A04790(TPHA0A04790)
TBLTBLA_0B09390(TBLA0B09390) TBLA_0H00760(TBLA0H00760)
TDLTDEL_0H00200(TDEL0H00200)
TGBHG536_0A04825 HG536_0F00230
ZMKHG535_0B00510
PPAPAS_chr3_0648
DHADEHA2F16654g
PICPICST_50942
PGUPGUG_04211
SPAASPAPADRAFT_51398
LELLELG_02871
CALCAALFM_C305130CA(THI4)
CTPCTRG_02525
COTCORT_0C04770
CDUCD36_85100
YLIYALI0A09768g
CLUCLUG_04547
CLUSA9F13_11g02431
CAURCJI97_003172
SLBAWJ20_4223(THI4)
PKZC5L36_0C00170
BNNFOA43_001955(STI35)
BBRXBRETT_001261(THI4)
NCRNCU06110
NTENEUTE1DRAFT95662(NEUTE1DRAFT_95662)
SMPSMAC_06409
PANPODANSg1687
TTTTHITE_2117402
MTMMYCTH_2315910
CTHRCTHT_0017160
MGRMGG_03098
TMNUCRPA7_2510
SSCKSPSK_07738
FGRFGSG_02469
FPUFPSE_01936
FVRFVEG_09077
FOXFOXG_10428
NHENECHADRAFT_61890
TRETRIREDRAFT_68608(thi4)
TRRM419DRAFT_89757
MAWMAC_01186
MAJMAA_05549
CMTCCM_01639
PLJVFPFJ_09336
VALVDBG_03908
VDAVDAG_01137
CFJCFIO01_04759
SAPOSAPIO_CDS2560
ELAUCREL1_6105
PFYPFICI_09188
SSLSS1G_09727
BFUBCIN_07g06010(Bcthi4)
MBEMBM_01288
PSCOLY89DRAFT_683526
GLZGLAREA_02091
ANIAN3928.2
AFMAFUA_6G08360
ACTACLA_082310
NFINFIA_054010
AORAO090003000090
ANGANI_1_228094(An11g01630)
AFVAFLA_001605
ALUCAKAW2_80142S(THI4)
ACHEACHE_80625S(THI4)
APUUAPUU_40431S(THI4)
PCSPc22g07960
PDPPDIP_32520
TMFPMAA_072920
TRGTRUGW13939_06486
CIMCIMG_01115
CPWCPC735_052570
UREUREG_01058
PBLPAAG_01125 PAAG_03817
PBNPADG_03708
ABEARB_07940
TVETRV_04098
AJEHCAG_05915
BGHBDBG_03342
PNOSNOG_05965
PTEPTT_08482
BZECOCCADRAFT_27023
BSCCOCSADRAFT_38262
BORCOCMIDRAFT_30031
AALTCC77DRAFT_1021441
ZTRMYCGRDRAFT_44262
PFJMYCFIDRAFT_211863
BCOMBAUCODRAFT_62660
NPAUCRNP2_7340
SPOSPBC26H8.01(thi2)
TVSTRAVEDRAFT_71517
DSQDICSQDRAFT_134894
PCOPHACADRAFT_177254
SHSSTEHIDRAFT_111603
HIRHETIRDRAFT_440039
PSQPUNSTDRAFT_42416
ADLAURDEDRAFT_139075
FMEFOMMEDRAFT_23696
GTRGLOTRDRAFT_77212
MRRMoror_11852
CCICC1G_04976
SCMSCHCODRAFT_84820
ABPAGABI1DRAFT40074(AGABI1DRAFT_40074)
ABVAGABI2DRAFT72787(AGABI2DRAFT_72787)
CPUTCONPUDRAFT_104209
SLASERLADRAFT_470126
WSEWALSEDRAFT_32181(CYPBP37)
WICJ056_001533
UMAUMAG_11400
PFPPFL1_06351
MGLMGL_1314
MSYMMSY001_3139
PGRPGTG_01304
MLRMELLADRAFT_53832
SMINv1.2.003820.t1(symbB.v1.2.003820.t1)
SPARSPRG_04526
GTTGUITHDRAFT_93981
 » show all
Reference
1  [PMID:16912043]
  Authors
Godoi PH, Galhardo RS, Luche DD, Van Sluys MA, Menck CF, Oliva G
  Title
Structure of the thiazole biosynthetic enzyme THI1 from Arabidopsis thaliana.
  Journal
J Biol Chem 281:30957-66 (2006)
DOI:10.1074/jbc.M604469200
  Sequence
[ath:AT5G54770]
Reference
2  [PMID:22031445]
  Authors
Chatterjee A, Abeydeera ND, Bale S, Pai PJ, Dorrestein PC, Russell DH, Ealick SE, Begley TP
  Title
Saccharomyces cerevisiae THI4p is a suicide thiamine thiazole synthase.
  Journal
Nature 478:542-6 (2011)
DOI:10.1038/nature10503
  Sequence
[sce:YGR144W]
Reference
3  [PMID:26919468]
  Authors
Zhang X, Eser BE, Chanani PK, Begley TP, Ealick SE
  Title
Structural Basis for Iron-Mediated Sulfur Transfer in Archael and Yeast Thiazole  Synthases.
  Journal
Biochemistry 55:1826-38 (2016)
DOI:10.1021/acs.biochem.6b00030
  Sequence
[sce:YGR144W]
Reference
4  [PMID:25348237]
  Authors
Hwang S, Cordova B, Chavarria N, Elbanna D, McHugh S, Rojas J, Pfeiffer F, Maupin-Furlow JA
  Title
Conserved active site cysteine residue of archaeal THI4 homolog is essential for thiamine biosynthesis in Haloferax volcanii.
  Journal
BMC Microbiol 14:260 (2014)
DOI:10.1186/s12866-014-0260-0
Other DBs
ExplorEnz - The Enzyme Database: 2.4.2.60
IUBMB Enzyme Nomenclature: 2.4.2.60
ExPASy - ENZYME nomenclature database: 2.4.2.60
BRENDA, the Enzyme Database: 2.4.2.60
LinkDB

KEGG   REACTION: R10685
Entry
R10685                      Reaction                               
Name
NAD+:glycine ADP-D-ribosyltransferase (dehydroalanine-producing)
Definition
NAD+ + Glycine + [Protein]-L-cysteine <=> ADP-5-ethyl-4-methylthiazole-2-carboxylate + Nicotinamide + [Protein]-dehydroalanine + 3 H2O
Equation
Comment
thiamine thiazole synthase
multi-step reaction
Sulfur donor is enzyme itself (one cystein residue looses H2S to form dehydroalanie residue)
Reaction class
RC00033  C00003_C00153
RC03253  C00003_C20784
RC03254  C00037_C20784
Enzyme
Pathway
rn00730  Thiamine metabolism
rn01100  Metabolic pathways
rn01240  Biosynthesis of cofactors
Module
M00897  Thiamine biosynthesis, plants, AIR (+ NAD+) => TMP/thiamine/TPP
M00898  Thiamine biosynthesis, pyridoxal-5P => TMP/thiamine/TPP
Orthology
K03146  cysteine-dependent adenosine diphosphate thiazole synthase [EC:2.4.2.60]
Other DBs
RHEA: 55711
LinkDB

KEGG   REACTION: R12026
Entry
R12026                      Reaction                               
Name
NAD+:glycine ADP-D-ribosyltransferase (sulfide-adding)
Definition
NAD+ + Glycine + Hydrogen sulfide <=> Nicotinamide + ADP-5-ethyl-4-methylthiazole-2-carboxylate + 3 H2O
Equation
Reaction class
RC00033  C00003_C00153
RC03253  C00003_C20784
RC03254  C00037_C20784
Enzyme
Pathway
rn00730  Thiamine metabolism
rn01100  Metabolic pathways
rn01240  Biosynthesis of cofactors
Module
M00896  Thiamine biosynthesis, archaea, AIR (+ NAD+) => TMP/TPP
Orthology
K22699  sulfide-dependent adenosine diphosphate thiazole synthase [EC:2.4.2.59]
Other DBs
RHEA: 55707
LinkDB

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