KEGG   ENZYME: 1.1.1.357
Entry
EC 1.1.1.357                Enzyme                                 

Name
3alpha-hydroxysteroid 3-dehydrogenase;
3alpha-hydroxysteroid dehydrogenase;
AKR1C4 (gene name);
AKR1C2 (gene name);
hsdA (gene name)
Class
Oxidoreductases;
Acting on the CH-OH group of donors;
With NAD+ or NADP+ as acceptor
Sysname
3alpha-hydroxysteroid:NAD(P)+ 3-oxidoreductase
Reaction(IUBMB)
a 3alpha-hydroxysteroid + NAD(P)+ = a 3-oxosteroid + NAD(P)H + H+ [RN:R10422 R10423]
Reaction(KEGG)
R10422 R10423
Substrate
3alpha-hydroxysteroid [CPD:C03072];
NAD+ [CPD:C00003];
NADP+ [CPD:C00006]
Product
3-oxosteroid [CPD:C01876];
NADH [CPD:C00004];
NADPH [CPD:C00005];
H+ [CPD:C00080]
Comment
The enzyme acts on multiple 3alpha-hydroxysteroids, such as androsterone and 5 alpha-dihydrotestosterone. The mammalian enzymes are involved in inactivation of steroid hormones, while the bacterial enzymes are involved in steroid degradation. This entry stands for enzymes whose stereo-specificity with respect to NAD+ or NADP+ is not known. [cf. EC 1.1.1.50, 3alpha-hydroxysteroid 3-dehydrogenase (Si-specific) and EC 1.1.1.213, 3alpha-hydroxysteroid 3-dehydrogenase (Re-specific)].
History
EC 1.1.1.357 created 2013
Orthology
K00037  3alpha-hydroxysteroid 3-dehydrogenase / chlordecone reductase
K00089  3alpha-hydroxysteroid 3-dehydrogenase
K00212  20alpha/3alpha-hydroxysteroid dehydrogenase / dihydrodiol dehydrogenase
K04119  aldo-keto reductase family 1 member C3
K19811  3alpha-hydroxysteroid 3-dehydrogenase
Genes
HSA: 1109(AKR1C4) 1645(AKR1C1) 1646(AKR1C2) 8644(AKR1C3)
PTR: 100607996 100612191(AKR1C2) 100612718(AKR1C3) 100612887 450274(AKR1C4) 746545
PPS: 100971300 100986388(AKR1C3) 100986729(AKR1C1) 100987632 100987989 100988315 103783188
GGO: 101144878 101146324 101147265 101148343 101152195 115936027
PON: 100171658(AKR1C1) 100174617(AKR1C3) 100189776 100432652 100434557(AKR1C4) 100461633 100462259
NLE: 100593325 100603603 100604844 100605385 100605934 100606512
MCC: 100430336 711198(AKR1C1) 711440 722485 722490(AKR1C4) 722496
MCF: 101865536(AKR1C1) 102116465(AKR1C2) 102117205(AKR1C4) 102142023(HSD17B5) 102143072 102144231
CSAB: 103237807 103237812 103237813 103237814 103237816
RRO: 104674747 104674748 104678924 104678925
RNO: 307092(Akr1c1) 361267(Akr1cl)
OCU: 100348607(PGER1) 100352716
CFA: 497070(AKR1C3)
VVP: 112933725
AML: 100483758
UMR: 103676234
UAH: 113241124
ELK: 111158636
FCA: 100510801(AKR1C3)
PTG: 102963188
PPAD: 109273789
AJU: 106988139
BTA: 100139386(20ALPHA-HSD) 112441538 112449293 282138(AKR1C4) 506594 527068 538060 617917(AKR1C3) 782884
SSC: 654406(AKR1CL1) 733634(AKR1C1) 733635 733694(AKR1C1)
LVE: 103069218
PCAD: 102978533
HAI: 109378613
CMY: 102946053
GJA: 107108026
XLA: 100049750(LOC100490956.L) 108712557(akr1c4.S) 443571(akr1c3.L)
CTES: O987_07590
 » show all
Reference
1  [PMID:8172617]
  Authors
Deyashiki Y, Ogasawara A, Nakayama T, Nakanishi M, Miyabe Y, Sato K, Hara A
  Title
Molecular cloning of two human liver 3 alpha-hydroxysteroid/dihydrodiol dehydrogenase isoenzymes that are identical with chlordecone reductase and bile-acid binder.
  Journal
Biochem J 299 ( Pt 2):545-52 (1994)
DOI:10.1042/bj2990545
  Sequence
[hsa:1645 1109]
Reference
2  [PMID:7650035]
  Authors
Khanna M, Qin KN, Wang RW, Cheng KC
  Title
Substrate specificity, gene structure, and tissue-specific distribution of multiple human 3 alpha-hydroxysteroid dehydrogenases.
  Journal
J Biol Chem 270:20162-8 (1995)
DOI:10.1074/jbc.270.34.20162
  Sequence
[hsa:8644]
Reference
3  [PMID:8944761]
  Authors
Oppermann UC, Maser E
  Title
Characterization of a 3 alpha-hydroxysteroid dehydrogenase/carbonyl reductase from the gram-negative bacterium Comamonas testosteroni.
  Journal
Eur J Biochem 241:744-9 (1996)
DOI:10.1111/j.1432-1033.1996.00744.x
  Sequence
Reference
4  [PMID:9812981]
  Authors
Mobus E, Maser E
  Title
Molecular cloning, overexpression, and characterization of steroid-inducible 3alpha-hydroxysteroid dehydrogenase/carbonyl reductase from Comamonas testosteroni. A novel member of the short-chain dehydrogenase/reductase superfamily.
  Journal
J Biol Chem 273:30888-96 (1998)
DOI:10.1074/jbc.273.47.30888
  Sequence
Reference
5  [PMID:11514561]
  Authors
Nahoum V, Gangloff A, Legrand P, Zhu DW, Cantin L, Zhorov BS, Luu-The V, Labrie F, Breton R, Lin SX
  Title
Structure of the human 3alpha-hydroxysteroid dehydrogenase type 3 in complex with testosterone and NADP at 1.25-A resolution.
  Journal
J Biol Chem 276:42091-8 (2001)
DOI:10.1074/jbc.M105610200
  Sequence
[hsa:1646]
Other DBs
ExplorEnz - The Enzyme Database: 1.1.1.357
IUBMB Enzyme Nomenclature: 1.1.1.357
ExPASy - ENZYME nomenclature database: 1.1.1.357
BRENDA, the Enzyme Database: 1.1.1.357
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