KEGG   ORTHOLOGY: K23558
Entry
K23558                      KO                                     
Symbol
3BETAHSDD
Name
plant 3beta-hydroxysteroid-4alpha-carboxylate 3-dehydrogenase [EC:1.1.1.418]
Pathway
map00100  Steroid biosynthesis
map01100  Metabolic pathways
map01110  Biosynthesis of secondary metabolites
Module
M00917  Phytosterol biosynthesis, squalene 2,3-epoxide => campesterol/sitosterol
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09103 Lipid metabolism
   00100 Steroid biosynthesis
    K23558  3BETAHSDD; plant 3beta-hydroxysteroid-4alpha-carboxylate 3-dehydrogenase
Enzymes [BR:ko01000]
 1. Oxidoreductases
  1.1  Acting on the CH-OH group of donors
   1.1.1  With NAD+ or NADP+ as acceptor
    1.1.1.418  plant 3beta-hydroxysteroid-4alpha-carboxylate 3-dehydrogenase (decarboxylating)
     K23558  3BETAHSDD; plant 3beta-hydroxysteroid-4alpha-carboxylate 3-dehydrogenase
Other DBs
RN: R12371 R12375 R12376
Genes
MMER: 123543975
ATH: AT1G47290(3BETAHSD/D1) AT2G26260(3BETAHSD/D2) AT2G43420
ALY: 9315018 9317969 9327409
CRB: 17888910 17889700 17898596
CSAT: 104703154 104714089 104752257 104758028 104777665 104782858 104785013 104793273 109124732
EUS: EUTSA_v10001381mg EUTSA_v10001949mg EUTSA_v10011495mg
BRP: 103842157 103843901 103866715
BNA: 106368540 106371310 106390851 106400657 106402151 106414952 106421095
BOE: 106296378 106307642 106308495 106341839
RSZ: 108811832 108818777 108856331 108862277 108862423
LJA: Lj0g3v0192789.1(Lj0g3v0192789.1) Lj2g3v0856600.1(Lj2g3v0856600.1) Lj3g3v3325980.1(Lj3g3v3325980.1)
FVE: 101292864
SLY: 101249150 101254144(HSD1) 101256443(HSD2)
DOSA: Os03t0404800-01(Os03g0404800) Os03t0405000-01(Os03g0405000) Os09t0516500-00(Os09g0516500)
PPP: 112282407
 » show all
Reference
  Authors
Rahier A, Darnet S, Bouvier F, Camara B, Bard M
  Title
Molecular and enzymatic characterizations of novel bifunctional 3beta-hydroxysteroid dehydrogenases/C-4 decarboxylases from Arabidopsis thaliana.
  Journal
J Biol Chem 281:27264-77 (2006)
DOI:10.1074/jbc.M604431200
  Sequence
Reference
  Authors
Rahier A, Bergdoll M, Genot G, Bouvier F, Camara B
  Title
Homology modeling and site-directed mutagenesis reveal catalytic key amino acids of 3beta-hydroxysteroid-dehydrogenase/C4-decarboxylase from Arabidopsis.
  Journal
Plant Physiol 149:1872-86 (2009)
DOI:10.1104/pp.108.132282
  Sequence
[ath:AT1G47290]
LinkDB

KEGG   ENZYME: 1.1.1.418
Entry
EC 1.1.1.418                Enzyme                                 
Name
plant 3beta-hydroxysteroid-4alpha-carboxylate 3-dehydrogenase (decarboxylating);
3beta-HSD/D1 (gene name);
3beta-HSD/D2 (gene name);
3beta-hydroxysteroid dehydrogenases/C-4 decarboxylase (ambiguous)
Class
Oxidoreductases;
Acting on the CH-OH group of donors;
With NAD+ or NADP+ as acceptor
Sysname
3beta-hydroxysteroid-4alpha-carboxylate:NAD+ 3-oxidoreductase (decarboxylating)
Reaction(IUBMB)
a 3beta-hydroxysteroid-4alpha-carboxylate + NAD+ = a 3-oxosteroid + CO2 + NADH [RN:R12366]
Reaction(KEGG)
Substrate
3beta-hydroxysteroid-4alpha-carboxylate [CPD:C22111];
NAD+ [CPD:C00003]
Product
3-oxosteroid [CPD:C01876];
CO2 [CPD:C00011];
NADH [CPD:C00004]
Comment
The enzyme, found in plants, catalyses multiple reactions during plant sterol biosynthesis. Unlike the fungal/animal enzyme EC 1.1.1.170, 3beta-hydroxysteroid-4alpha-carboxylate 3-dehydrogenase (decarboxylating), the plant enzyme is specific for NAD+.
History
EC 1.1.1.418 created 2019
Pathway
ec00100  Steroid biosynthesis
ec01100  Metabolic pathways
ec01110  Biosynthesis of secondary metabolites
Orthology
K23558  plant 3beta-hydroxysteroid-4alpha-carboxylate 3-dehydrogenase
Genes
MMER123543975
ATHAT1G47290(3BETAHSD/D1) AT2G26260(3BETAHSD/D2) AT2G43420
ALY9315018 9317969 9327409
CRB17888910 17889700 17898596
CSAT104703154 104714089 104752257 104758028 104777665 104782858 104785013 104793273 109124732
EUSEUTSA_v10001381mg EUTSA_v10001949mg EUTSA_v10011495mg
BRP103842157 103843901 103866715
BNA106368540 106371310 106390851 106400657 106402151 106414952 106421095
BOE106296378 106307642 106308495 106341839
RSZ108811832 108818777 108856331 108862277 108862423
THJ104802316 104803571 104804450 104819910 104819911
CPAP110812016 110821405
CIT102607843 102626005
CICCICLE_v10007884mg CICLE_v10019433mg
PVY116117123 116132027 116145720
MINC123194436 123206420 123221140
TCC18588175 18602449
GRA105766177 105773475 105778202 105796377
GHI107896916 107901017 107903885 107914644 107927476 107938719 107957226 107961633
GAB108476304 108483574 108488634
DZI111279431 111285384 111303015 111307734
EGR104435786 104436360
GMX100794808 100799363 100800800 100809319 100814147 100815785 100816854
GSJ114374173 114379749 114405678 114410541 114410545 114414874 114418722
PVUPHAVU_009G084200g PHAVU_011G053200g
VRA106756699 106760856 106776089
VAR108330047 108340625
VUN114164232 114170010
CCAJ109791368 109801541
APRC113864705 113865991
MTR11412484 11426731
CAM101494810 101500996
LJALj0g3v0192789.1(Lj0g3v0192789.1) Lj2g3v0856600.1(Lj2g3v0856600.1) Lj3g3v3325980.1(Lj3g3v3325980.1)
ADU107470664 107470953 107476756
AIP107620898 107623291 107628972
AHF112716044 112716215 112736795 112783352 112783971 114927374
LANG109325732 109328420 109341751 109343798
FVE101292864
RCN112171861 112199072
PPER18791941 18793760
PMUM103320667 103322301
PAVI110749482 110773108
PDUL117614277 117614519
MDM103419184 103440612
PXB103933227 103949660 103953014
ZJU107405093 107420046 107422105
MNT21384233 21399197 21401592
CSV101205275 101212494
CMO103483967 103488530
BHJ120075097 120085819
MCHA111004984 111009273
CMAX111470875 111493953
CMOS111439089 111450770
CPEP111801021 111805666
RCU8258610 8287636
JCU105628414 105638060
HBR110639226 110662259
MESC110604012 110613045
POP18100844 7492281
PEU105113088 105116056 105122098
PALZ118030630 118032037
JRE108985075 108990435
QSU111989557 112029405
QLO115952975 115963676 115991809
TWL119988085 119997351
VVI100245662 100257631
VRI117913150 117915011
SLY101249150 101254144(HSD1) 101256443(HSD2)
SPEN107005180 107011682 107011815
SOT102587792 102596460 102598251
SSTN125848854 125849035 125863127
CANN107857986 107859693 107863753
NTA107780439 107795088 107795373 107814161 107817215 107818175
NSY104216879 104239655 104245967
NTO104084496 104092502 104108740
NAU109210739 109213017 109227010
INI109161741 109184677
ITR116017169 116025787
SIND105164848 105165847 105172010
OEU111371187 111377242 111377243 111393378
EGT105953922 105967180 105975202
SSPL121744496 121745522 121775340 121776174 121778525 121780910 121789729 121790191 121811147
HAN110899855 110908422 110917497 110920605
ECAD122587238 122600031 122606208 122608089
LSV111875990 111883224 111899990 111919546
CCAV112503045 112508871 112511529 112522090
DCR108197059 108197988 108203699 108217750 108223476
CSIN114279462 114310008 114313733 114320505
BVG104888851 104904629
SOE110787809 110802274
CQI110682749 110696539 110717644 110738779
NNU104588912 104605277
MING122089108 122093861
TSS122640370 122656788
PSOM113297813 113300464 113301315 113328380 113348744
NCOL116246422 116248785 116267192
OSA4333070 4333071 4347580
DOSAOs03t0404800-01(Os03g0404800) Os03t0405000-01(Os03g0405000) Os09t0516500-00(Os09g0516500)
OBR102699988 102711055 102711335
BDI100832384 100838723 100841751
ATS109751859 109759047 109759249 109776475 109780969 109782739
TDC119285843 119296052 119296770 119299834 119304981 119311150 119324550 119332326 119339590
TAES123085918 123091921 123097331 123104417 123106270 123112715 123119045 123122193 123124996 123141346 123149698 123158639 123161387
TUA125529639 125550654
SBI110431777 110431804 8063889 8077068 8077069 8077070 8077071 8077107 8077112
ZMA100283692 100383338 103647586
SITA101762396 101776881 101778928 101779594 101779992
SVS117835670 117836785 117836879 117836880 117836994 117838718 117846541
PVIR120652105 120652106 120661244 120684071 120690955
PHAI112877458 112881953
PDA103702195 103706039 120103893
EGU105035203 105056704
MUS103968507 103992669
DCT110109494 110113291
PEQ110038378 110039408
AOF109829223 109838803 109844846
ATR18431743 18433647 18448731
SMOSELMODRAFT_129445 SELMODRAFT_84229
PPP112282407
 » show all
Reference
1  [PMID:10356290]
  Authors
Rondet S, Taton M, Rahier A
  Title
Identification, characterization, and partial purification of 4 alpha-carboxysterol-C3-dehydrogenase/ C4-decarboxylase from Zea mays.
  Journal
Arch Biochem Biophys 366:249-60 (1999)
DOI:10.1006/abbi.1999.1218
Reference
2  [PMID:16835224]
  Authors
Rahier A, Darnet S, Bouvier F, Camara B, Bard M
  Title
Molecular and enzymatic characterizations of novel bifunctional 3beta-hydroxysteroid dehydrogenases/C-4 decarboxylases from Arabidopsis thaliana.
  Journal
J Biol Chem 281:27264-77 (2006)
DOI:10.1074/jbc.M604431200
  Sequence
Reference
3  [PMID:19218365]
  Authors
Rahier A, Bergdoll M, Genot G, Bouvier F, Camara B
  Title
Homology modeling and site-directed mutagenesis reveal catalytic key amino acids of 3beta-hydroxysteroid-dehydrogenase/C4-decarboxylase from Arabidopsis.
  Journal
Plant Physiol 149:1872-86 (2009)
DOI:10.1104/pp.108.132282
  Sequence
[ath:AT1G47290]
Other DBs
ExplorEnz - The Enzyme Database: 1.1.1.418
IUBMB Enzyme Nomenclature: 1.1.1.418
ExPASy - ENZYME nomenclature database: 1.1.1.418
BRENDA, the Enzyme Database: 1.1.1.418
CAS: 71822-23-6
LinkDB

KEGG   REACTION: R12376
Entry
R12376                      Reaction                               
Name
4alpha-carboxy-stigmasta-7,24(24(1))-dien-3beta-ol:NAD+ 3-oxidoreductase (decarboxylating)
Definition
4alpha-Carboxy-stigmasta-7,24(24(1))-dien-3beta-ol + NAD+ <=> Avenastenone + NADH + CO2 + H+
Equation
Reaction class
RC03536  C22120_C22123
Enzyme
Pathway
rn00100  Steroid biosynthesis
rn01100  Metabolic pathways
rn01110  Biosynthesis of secondary metabolites
Module
M00917  Phytosterol biosynthesis, squalene 2,3-epoxide => campesterol/sitosterol
Orthology
K23558  plant 3beta-hydroxysteroid-4alpha-carboxylate 3-dehydrogenase [EC:1.1.1.418]
LinkDB

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