KEGG   ORTHOLOGY: K04126
Entry
K04126                      KO                                     
Symbol
PCBC
Name
isopenicillin-N synthase [EC:1.21.3.1]
Pathway
map00311  Penicillin and cephalosporin biosynthesis
map01100  Metabolic pathways
map01110  Biosynthesis of secondary metabolites
Module
M00672  Penicillin biosynthesis, aminoadipate + cycteine + valine => penicillin
M00673  Cephamycin C biosynthesis, aminoadipate + cycteine + valine => cephamycin C
Reaction
R04872  
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09110 Biosynthesis of other secondary metabolites
   00311 Penicillin and cephalosporin biosynthesis
    K04126  PCBC; isopenicillin-N synthase
 09180 Brite Hierarchies
  09181 Protein families: metabolism
   01008 Polyketide biosynthesis proteins
    K04126  PCBC; isopenicillin-N synthase
Enzymes [BR:ko01000]
 1. Oxidoreductases
  1.21  Catalysing the reaction X-H + Y-H = X-Y
   1.21.3  With oxygen as acceptor
    1.21.3.1  isopenicillin-N synthase
     K04126  PCBC; isopenicillin-N synthase
Polyketide biosynthesis proteins [BR:ko01008]
 Nonribosomal peptide tailoring proteins
  Others
   K04126  PCBC; isopenicillin-N synthase
Other DBs
GO: 0016216
Genes
BCOO: 119077147
FCD: 110843583
PMEO: 129593743
PCHM: VFPPC_08656
ANI: ANIA_02622
AOR: AO090038000544
AFV: AFLA_009030
APUU: APUU_12363A
PCS: N7525_006405
ABE: ARB_02225
TVE: TRV_00552
XRY: QN244_11745
SCLF: BB341_07795
STIR: DDW44_23090
SCT: SCAT_5683(pcbC)
SCY: SCATT_56830
AAB: A4R43_03225
AMYY: YIM_39180(cefE1)
PSUU: Psuf_059690
 » show all
Reference
PMID:7791906
  Authors
Roach PL, Clifton IJ, Fulop V, Harlos K, Barton GJ, Hajdu J, Andersson I, Schofield CJ, Baldwin JE
  Title
Crystal structure of isopenicillin N synthase is the first from a new structural family of enzymes.
  Journal
Nature 375:700-4 (1995)
DOI:10.1038/375700a0
  Sequence
[ani:ANIA_02622]
LinkDB

KEGG   ENZYME: 1.21.3.1
Entry
EC 1.21.3.1                 Enzyme                                 
Name
isopenicillin-N synthase;
isopenicillin N synthetase
Class
Oxidoreductases;
Catalysing the reaction X-H + Y-H = X-Y;
With oxygen as acceptor
Sysname
N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine:oxygen oxidoreductase (cyclizing)
Reaction(IUBMB)
N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine + O2 = isopenicillin N + 2 H2O
Reaction(KEGG)
(other) R04872
Substrate
N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine;
O2 [CPD:C00007]
Product
isopenicillin N [CPD:C05557];
H2O [CPD:C00001]
Comment
Forms part of the penicillin biosynthesis pathway (for pathway, click here).
History
EC 1.21.3.1 created 2002
Pathway
ec00311  Penicillin and cephalosporin biosynthesis
ec01100  Metabolic pathways
ec01110  Biosynthesis of secondary metabolites
Orthology
K04126  isopenicillin-N synthase
Genes
BCOO: 119077147
FCD: 110843583
PMEO: 129593743
PCHM: VFPPC_08656
ANI: ANIA_02622
AOR: AO090038000544
AFV: AFLA_009030
APUU: APUU_12363A
PCS: N7525_006405
ABE: ARB_02225
TVE: TRV_00552
XRY: QN244_11745
SCLF: BB341_07795
STIR: DDW44_23090
SCT: SCAT_5683(pcbC)
SCY: SCATT_56830
AAB: A4R43_03225
AMYY: YIM_39180(cefE1)
PSUU: Psuf_059690
 » show all
Reference
1  [PMID:1588566]
  Authors
Huffman GW, Gesellchen PD, Turner JR, Rothenberger RB, Osborne HE, Miller FD, Chapman JL, Queener SW.
  Title
Substrate specificity of isopenicillin N synthase.
  Journal
J Med Chem 35:1897-914 (1992)
DOI:10.1021/jm00088a028
Reference
2  [PMID:7791906]
  Authors
Roach PL, Clifton IJ, Fulop V, Harlos K, Barton GJ, Hajdu J, Andersson I, Schofield CJ, Baldwin JE
  Title
Crystal structure of isopenicillin N synthase is the first from a new structural family of enzymes.
  Journal
Nature 375:700-4 (1995)
DOI:10.1038/375700a0
  Sequence
[ani:ANIA_02622]
Other DBs
ExplorEnz - The Enzyme Database: 1.21.3.1
IUBMB Enzyme Nomenclature: 1.21.3.1
ExPASy - ENZYME nomenclature database: 1.21.3.1
BRENDA, the Enzyme Database: 1.21.3.1
CAS: 78642-31-6
LinkDB

KEGG   REACTION: R04872
Entry
R04872                      Reaction                               
Definition
delta-(L-2-Aminoadipyl)-L-cysteinyl-D-valine + Oxygen <=> Isopenicillin N + 2 H2O
Equation
C05556 + C00007 <=> C05557 + 2 C00001
Comment
multi-step reaction
Reaction class
RC01229  C05556_C05557
Enzyme
1.21.3.1
Pathway
rn00311  Penicillin and cephalosporin biosynthesis
rn01100  Metabolic pathways
rn01110  Biosynthesis of secondary metabolites
Module
M00672  Penicillin biosynthesis, aminoadipate + cycteine + valine => penicillin
M00673  Cephamycin C biosynthesis, aminoadipate + cycteine + valine => cephamycin C
Brite
Enzymatic reactions [BR:br08201]
 1. Oxidoreductase reactions
  1.21  Catalysing the reaction X-H + Y-H = X-Y
   1.21.3  With oxygen as acceptor
    1.21.3.1
     R04872  delta-(L-2-Aminoadipyl)-L-cysteinyl-D-valine + Oxygen <=> Isopenicillin N + 2 H2O
Orthology
K04126  isopenicillin-N synthase [EC:1.21.3.1]
Other DBs
RHEA: 22431
LinkDB

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